Amino acid dipepetide frequency for Rubrobacter taiwanensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.443AlaAla: 16.443 ± 0.203	0.862AlaCys: 0.862 ± 0.034	4.475AlaAsp: 4.475 ± 0.076	8.179AlaGlu: 8.179 ± 0.108	3.875AlaPhe: 3.875 ± 0.078	12.819AlaGly: 12.819 ± 0.153	1.697AlaHis: 1.697 ± 0.042	4.594AlaIle: 4.594 ± 0.075	2.016AlaLys: 2.016 ± 0.05	13.454AlaLeu: 13.454 ± 0.166	2.3AlaMet: 2.3 ± 0.048	1.991AlaAsn: 1.991 ± 0.042	4.644AlaPro: 4.644 ± 0.077	2.411AlaGln: 2.411 ± 0.057	9.991AlaArg: 9.991 ± 0.122	4.963AlaSer: 4.963 ± 0.066	4.465AlaThr: 4.465 ± 0.084	9.957AlaVal: 9.957 ± 0.126	1.118AlaTrp: 1.118 ± 0.038	2.797AlaTyr: 2.797 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.799CysAla: 0.799 ± 0.026	0.119CysCys: 0.119 ± 0.01	0.341CysAsp: 0.341 ± 0.019	0.56CysGlu: 0.56 ± 0.027	0.228CysPhe: 0.228 ± 0.016	1.054CysGly: 1.054 ± 0.032	0.162CysHis: 0.162 ± 0.014	0.322CysIle: 0.322 ± 0.019	0.14CysLys: 0.14 ± 0.012	0.64CysLeu: 0.64 ± 0.027	0.156CysMet: 0.156 ± 0.013	0.157CysAsn: 0.157 ± 0.012	0.47CysPro: 0.47 ± 0.024	0.133CysGln: 0.133 ± 0.013	0.646CysArg: 0.646 ± 0.028	0.397CysSer: 0.397 ± 0.021	0.346CysThr: 0.346 ± 0.02	0.572CysVal: 0.572 ± 0.03	0.081CysTrp: 0.081 ± 0.01	0.217CysTyr: 0.217 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.995AspAla: 4.995 ± 0.073	0.279AspCys: 0.279 ± 0.017	1.852AspAsp: 1.852 ± 0.053	4.004AspGlu: 4.004 ± 0.069	1.746AspPhe: 1.746 ± 0.043	4.333AspGly: 4.333 ± 0.08	0.877AspHis: 0.877 ± 0.029	2.076AspIle: 2.076 ± 0.046	0.705AspLys: 0.705 ± 0.028	5.387AspLeu: 5.387 ± 0.071	0.796AspMet: 0.796 ± 0.031	0.726AspAsn: 0.726 ± 0.026	3.634AspPro: 3.634 ± 0.072	0.818AspGln: 0.818 ± 0.03	4.206AspArg: 4.206 ± 0.071	1.894AspSer: 1.894 ± 0.05	1.877AspThr: 1.877 ± 0.041	3.705AspVal: 3.705 ± 0.07	0.685AspTrp: 0.685 ± 0.027	1.369AspTyr: 1.369 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	9.354GluAla: 9.354 ± 0.13	0.475GluCys: 0.475 ± 0.024	3.972GluAsp: 3.972 ± 0.062	8.624GluGlu: 8.624 ± 0.12	2.37GluPhe: 2.37 ± 0.057	6.548GluGly: 6.548 ± 0.085	1.681GluHis: 1.681 ± 0.042	4.398GluIle: 4.398 ± 0.077	2.296GluLys: 2.296 ± 0.062	9.256GluLeu: 9.256 ± 0.115	1.643GluMet: 1.643 ± 0.04	1.868GluAsn: 1.868 ± 0.042	3.983GluPro: 3.983 ± 0.076	2.06GluGln: 2.06 ± 0.054	9.141GluArg: 9.141 ± 0.114	3.599GluSer: 3.599 ± 0.065	3.197GluThr: 3.197 ± 0.064	7.013GluVal: 7.013 ± 0.103	0.978GluTrp: 0.978 ± 0.033	2.003GluTyr: 2.003 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	4.045PheAla: 4.045 ± 0.077	0.346PheCys: 0.346 ± 0.018	1.842PheAsp: 1.842 ± 0.045	2.589PheGlu: 2.589 ± 0.054	1.557PhePhe: 1.557 ± 0.049	3.829PheGly: 3.829 ± 0.075	0.612PheHis: 0.612 ± 0.025	1.507PheIle: 1.507 ± 0.044	0.701PheLys: 0.701 ± 0.024	3.612PheLeu: 3.612 ± 0.076	0.681PheMet: 0.681 ± 0.031	0.78PheAsn: 0.78 ± 0.027	1.627PhePro: 1.627 ± 0.044	0.704PheGln: 0.704 ± 0.029	2.59PheArg: 2.59 ± 0.055	1.816PheSer: 1.816 ± 0.046	1.763PheThr: 1.763 ± 0.048	2.994PheVal: 2.994 ± 0.068	0.535PheTrp: 0.535 ± 0.027	1.048PheTyr: 1.048 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	10.041GlyAla: 10.041 ± 0.135	0.905GlyCys: 0.905 ± 0.033	4.276GlyAsp: 4.276 ± 0.067	8.422GlyGlu: 8.422 ± 0.108	3.589GlyPhe: 3.589 ± 0.064	9.325GlyGly: 9.325 ± 0.119	1.69GlyHis: 1.69 ± 0.041	4.854GlyIle: 4.854 ± 0.067	2.39GlyLys: 2.39 ± 0.059	9.401GlyLeu: 9.401 ± 0.119	2.497GlyMet: 2.497 ± 0.044	1.719GlyAsn: 1.719 ± 0.039	3.978GlyPro: 3.978 ± 0.074	1.989GlyGln: 1.989 ± 0.045	8.45GlyArg: 8.45 ± 0.104	5.039GlySer: 5.039 ± 0.09	4.477GlyThr: 4.477 ± 0.069	8.261GlyVal: 8.261 ± 0.098	1.403GlyTrp: 1.403 ± 0.04	2.881GlyTyr: 2.881 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.849HisAla: 1.849 ± 0.046	0.18HisCys: 0.18 ± 0.013	0.788HisAsp: 0.788 ± 0.029	1.238HisGlu: 1.238 ± 0.035	0.607HisPhe: 0.607 ± 0.024	1.696HisGly: 1.696 ± 0.042	0.476HisHis: 0.476 ± 0.025	0.791HisIle: 0.791 ± 0.026	0.309HisLys: 0.309 ± 0.017	2.028HisLeu: 2.028 ± 0.048	0.309HisMet: 0.309 ± 0.019	0.432HisAsn: 0.432 ± 0.021	1.482HisPro: 1.482 ± 0.044	0.402HisGln: 0.402 ± 0.022	1.684HisArg: 1.684 ± 0.045	0.814HisSer: 0.814 ± 0.028	0.869HisThr: 0.869 ± 0.031	1.301HisVal: 1.301 ± 0.036	0.197HisTrp: 0.197 ± 0.014	0.568HisTyr: 0.568 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	4.741IleAla: 4.741 ± 0.088	0.382IleCys: 0.382 ± 0.019	1.911IleAsp: 1.911 ± 0.05	3.495IleGlu: 3.495 ± 0.065	1.77IlePhe: 1.77 ± 0.052	4.017IleGly: 4.017 ± 0.076	0.79IleHis: 0.79 ± 0.028	1.983IleIle: 1.983 ± 0.058	0.983IleLys: 0.983 ± 0.035	4.876IleLeu: 4.876 ± 0.076	0.788IleMet: 0.788 ± 0.029	1.018IleAsn: 1.018 ± 0.036	2.793IlePro: 2.793 ± 0.051	1.024IleGln: 1.024 ± 0.035	3.676IleArg: 3.676 ± 0.063	3.104IleSer: 3.104 ± 0.065	2.427IleThr: 2.427 ± 0.061	3.497IleVal: 3.497 ± 0.065	0.468IleTrp: 0.468 ± 0.024	1.221IleTyr: 1.221 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	2.14LysAla: 2.14 ± 0.045	0.121LysCys: 0.121 ± 0.01	1.05LysAsp: 1.05 ± 0.04	1.818LysGlu: 1.818 ± 0.04	0.599LysPhe: 0.599 ± 0.026	1.741LysGly: 1.741 ± 0.051	0.424LysHis: 0.424 ± 0.022	1.277LysIle: 1.277 ± 0.041	0.778LysLys: 0.778 ± 0.032	2.327LysLeu: 2.327 ± 0.055	0.556LysMet: 0.556 ± 0.026	0.607LysAsn: 0.607 ± 0.028	1.286LysPro: 1.286 ± 0.034	0.549LysGln: 0.549 ± 0.028	2.117LysArg: 2.117 ± 0.051	1.218LysSer: 1.218 ± 0.039	1.271LysThr: 1.271 ± 0.041	1.997LysVal: 1.997 ± 0.057	0.25LysTrp: 0.25 ± 0.016	0.555LysTyr: 0.555 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	13.59LeuAla: 13.59 ± 0.161	0.797LeuCys: 0.797 ± 0.029	5.145LeuAsp: 5.145 ± 0.072	8.855LeuGlu: 8.855 ± 0.115	3.744LeuPhe: 3.744 ± 0.083	9.999LeuGly: 9.999 ± 0.12	1.768LeuHis: 1.768 ± 0.042	4.54LeuIle: 4.54 ± 0.092	2.997LeuLys: 2.997 ± 0.062	11.506LeuLeu: 11.506 ± 0.168	1.982LeuMet: 1.982 ± 0.045	2.239LeuAsn: 2.239 ± 0.053	5.821LeuPro: 5.821 ± 0.088	2.485LeuGln: 2.485 ± 0.055	9.17LeuArg: 9.17 ± 0.11	6.077LeuSer: 6.077 ± 0.078	4.579LeuThr: 4.579 ± 0.08	8.336LeuVal: 8.336 ± 0.115	1.238LeuTrp: 1.238 ± 0.041	2.783LeuTyr: 2.783 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.083MetAla: 2.083 ± 0.05	0.12MetCys: 0.12 ± 0.011	0.93MetAsp: 0.93 ± 0.036	1.444MetGlu: 1.444 ± 0.044	0.543MetPhe: 0.543 ± 0.025	1.735MetGly: 1.735 ± 0.046	0.339MetHis: 0.339 ± 0.019	1.009MetIle: 1.009 ± 0.032	0.683MetLys: 0.683 ± 0.026	2.159MetLeu: 2.159 ± 0.05	0.465MetMet: 0.465 ± 0.022	0.595MetAsn: 0.595 ± 0.024	1.102MetPro: 1.102 ± 0.036	0.656MetGln: 0.656 ± 0.028	1.893MetArg: 1.893 ± 0.045	1.351MetSer: 1.351 ± 0.038	1.102MetThr: 1.102 ± 0.03	1.602MetVal: 1.602 ± 0.045	0.213MetTrp: 0.213 ± 0.015	0.376MetTyr: 0.376 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.205AsnAla: 2.205 ± 0.048	0.184AsnCys: 0.184 ± 0.014	0.782AsnAsp: 0.782 ± 0.027	1.191AsnGlu: 1.191 ± 0.034	0.805AsnPhe: 0.805 ± 0.029	1.714AsnGly: 1.714 ± 0.046	0.395AsnHis: 0.395 ± 0.021	1.048AsnIle: 1.048 ± 0.033	0.363AsnLys: 0.363 ± 0.021	2.524AsnLeu: 2.524 ± 0.049	0.398AsnMet: 0.398 ± 0.019	0.471AsnAsn: 0.471 ± 0.025	1.74AsnPro: 1.74 ± 0.044	0.476AsnGln: 0.476 ± 0.023	1.772AsnArg: 1.772 ± 0.046	0.924AsnSer: 0.924 ± 0.034	1.046AsnThr: 1.046 ± 0.036	1.825AsnVal: 1.825 ± 0.045	0.265AsnTrp: 0.265 ± 0.017	0.601AsnTyr: 0.601 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.898ProAla: 5.898 ± 0.091	0.289ProCys: 0.289 ± 0.019	3.452ProAsp: 3.452 ± 0.069	6.686ProGlu: 6.686 ± 0.081	1.784ProPhe: 1.784 ± 0.049	5.893ProGly: 5.893 ± 0.096	1.054ProHis: 1.054 ± 0.037	1.626ProIle: 1.626 ± 0.042	1.05ProLys: 1.05 ± 0.035	5.058ProLeu: 5.058 ± 0.077	0.871ProMet: 0.871 ± 0.031	1.062ProAsn: 1.062 ± 0.034	3.146ProPro: 3.146 ± 0.063	1.388ProGln: 1.388 ± 0.039	3.465ProArg: 3.465 ± 0.061	2.042ProSer: 2.042 ± 0.049	1.9ProThr: 1.9 ± 0.047	4.918ProVal: 4.918 ± 0.079	0.66ProTrp: 0.66 ± 0.029	1.414ProTyr: 1.414 ± 0.038	0.001ProXaa: 0.001 ± 0.001
Gln	2.526GlnAla: 2.526 ± 0.059	0.102GlnCys: 0.102 ± 0.01	1.073GlnAsp: 1.073 ± 0.031	2.048GlnGlu: 2.048 ± 0.05	0.664GlnPhe: 0.664 ± 0.026	1.709GlnGly: 1.709 ± 0.042	0.416GlnHis: 0.416 ± 0.022	1.322GlnIle: 1.322 ± 0.035	0.721GlnLys: 0.721 ± 0.028	2.274GlnLeu: 2.274 ± 0.051	0.547GlnMet: 0.547 ± 0.024	0.653GlnAsn: 0.653 ± 0.028	1.057GlnPro: 1.057 ± 0.04	0.84GlnGln: 0.84 ± 0.04	2.168GlnArg: 2.168 ± 0.054	1.055GlnSer: 1.055 ± 0.03	1.162GlnThr: 1.162 ± 0.035	1.892GlnVal: 1.892 ± 0.041	0.251GlnTrp: 0.251 ± 0.018	0.596GlnTyr: 0.596 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	9.199ArgAla: 9.199 ± 0.12	0.642ArgCys: 0.642 ± 0.027	4.05ArgAsp: 4.05 ± 0.063	8.947ArgGlu: 8.947 ± 0.114	3.108ArgPhe: 3.108 ± 0.049	7.005ArgGly: 7.005 ± 0.091	1.692ArgHis: 1.692 ± 0.044	4.046ArgIle: 4.046 ± 0.071	2.253ArgLys: 2.253 ± 0.05	9.399ArgLeu: 9.399 ± 0.124	2.076ArgMet: 2.076 ± 0.047	1.839ArgAsn: 1.839 ± 0.041	4.46ArgPro: 4.46 ± 0.068	2.308ArgGln: 2.308 ± 0.052	9.773ArgArg: 9.773 ± 0.146	4.517ArgSer: 4.517 ± 0.072	3.692ArgThr: 3.692 ± 0.064	7.164ArgVal: 7.164 ± 0.1	1.171ArgTrp: 1.171 ± 0.034	2.533ArgTyr: 2.533 ± 0.059	0.0ArgXaa: 0.0 ± 0.0
Ser	5.041SerAla: 5.041 ± 0.081	0.392SerCys: 0.392 ± 0.018	2.144SerAsp: 2.144 ± 0.05	3.674SerGlu: 3.674 ± 0.057	1.957SerPhe: 1.957 ± 0.052	6.504SerGly: 6.504 ± 0.082	0.879SerHis: 0.879 ± 0.033	2.101SerIle: 2.101 ± 0.056	0.96SerLys: 0.96 ± 0.034	4.994SerLeu: 4.994 ± 0.075	1.129SerMet: 1.129 ± 0.035	0.929SerAsn: 0.929 ± 0.032	3.051SerPro: 3.051 ± 0.061	1.064SerGln: 1.064 ± 0.031	4.403SerArg: 4.403 ± 0.07	2.577SerSer: 2.577 ± 0.068	2.305SerThr: 2.305 ± 0.048	4.0SerVal: 4.0 ± 0.064	0.726SerTrp: 0.726 ± 0.029	1.379SerTyr: 1.379 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	4.747ThrAla: 4.747 ± 0.071	0.283ThrCys: 0.283 ± 0.019	1.954ThrAsp: 1.954 ± 0.045	2.601ThrGlu: 2.601 ± 0.052	1.779ThrPhe: 1.779 ± 0.039	5.183ThrGly: 5.183 ± 0.067	0.817ThrHis: 0.817 ± 0.032	1.933ThrIle: 1.933 ± 0.045	0.802ThrLys: 0.802 ± 0.035	5.219ThrLeu: 5.219 ± 0.065	0.845ThrMet: 0.845 ± 0.035	0.955ThrAsn: 0.955 ± 0.034	2.897ThrPro: 2.897 ± 0.052	0.915ThrGln: 0.915 ± 0.032	3.244ThrArg: 3.244 ± 0.053	2.174ThrSer: 2.174 ± 0.047	2.091ThrThr: 2.091 ± 0.056	4.108ThrVal: 4.108 ± 0.082	0.441ThrTrp: 0.441 ± 0.022	1.223ThrTyr: 1.223 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	9.409ValAla: 9.409 ± 0.122	0.755ValCys: 0.755 ± 0.026	3.978ValAsp: 3.978 ± 0.069	7.088ValGlu: 7.088 ± 0.11	3.017ValPhe: 3.017 ± 0.065	6.857ValGly: 6.857 ± 0.115	1.44ValHis: 1.44 ± 0.04	3.901ValIle: 3.901 ± 0.069	1.761ValLys: 1.761 ± 0.05	9.198ValLeu: 9.198 ± 0.123	1.608ValMet: 1.608 ± 0.041	1.776ValAsn: 1.776 ± 0.045	4.452ValPro: 4.452 ± 0.076	1.88ValGln: 1.88 ± 0.052	7.656ValArg: 7.656 ± 0.103	4.58ValSer: 4.58 ± 0.081	3.722ValThr: 3.722 ± 0.059	7.551ValVal: 7.551 ± 0.103	1.0ValTrp: 1.0 ± 0.034	2.06ValTyr: 2.06 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	1.128TrpAla: 1.128 ± 0.032	0.101TrpCys: 0.101 ± 0.01	0.578TrpAsp: 0.578 ± 0.025	0.948TrpGlu: 0.948 ± 0.036	0.432TrpPhe: 0.432 ± 0.022	0.996TrpGly: 0.996 ± 0.035	0.263TrpHis: 0.263 ± 0.017	0.595TrpIle: 0.595 ± 0.025	0.337TrpLys: 0.337 ± 0.017	1.316TrpLeu: 1.316 ± 0.04	0.31TrpMet: 0.31 ± 0.017	0.349TrpAsn: 0.349 ± 0.02	0.565TrpPro: 0.565 ± 0.022	0.377TrpGln: 0.377 ± 0.022	1.168TrpArg: 1.168 ± 0.038	0.726TrpSer: 0.726 ± 0.03	0.593TrpThr: 0.593 ± 0.026	0.946TrpVal: 0.946 ± 0.039	0.248TrpTrp: 0.248 ± 0.017	0.307TrpTyr: 0.307 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.819TyrAla: 2.819 ± 0.056	0.207TyrCys: 0.207 ± 0.014	1.373TyrAsp: 1.373 ± 0.042	2.046TyrGlu: 2.046 ± 0.051	1.007TyrPhe: 1.007 ± 0.031	2.628TyrGly: 2.628 ± 0.049	0.548TyrHis: 0.548 ± 0.025	1.037TyrIle: 1.037 ± 0.036	0.438TyrLys: 0.438 ± 0.024	3.027TyrLeu: 3.027 ± 0.064	0.442TyrMet: 0.442 ± 0.024	0.563TyrAsn: 0.563 ± 0.025	1.47TyrPro: 1.47 ± 0.042	0.596TyrGln: 0.596 ± 0.027	2.662TyrArg: 2.662 ± 0.059	1.339TyrSer: 1.339 ± 0.04	1.326TyrThr: 1.326 ± 0.038	2.05TyrVal: 2.05 ± 0.046	0.354TyrTrp: 0.354 ± 0.02	0.832TyrTyr: 0.832 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3014 proteins (923775 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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