Leekyejoonella antrihumi
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4772 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563DXG4|A0A563DXG4_9MICO FtsX-like permease family protein OS=Leekyejoonella antrihumi OX=1660198 GN=FGL98_15515 PE=4 SV=1
MM1 pKa = 7.76 TDD3 pKa = 2.6 WQSAFNEE10 pKa = 4.28 FLSTDD15 pKa = 3.81 PTDD18 pKa = 3.75 VGCDD22 pKa = 2.81 EE23 pKa = 4.43 AMRR26 pKa = 11.84 VLEE29 pKa = 4.3 VYY31 pKa = 10.72 VDD33 pKa = 4.17 LVSTGLDD40 pKa = 3.09 AAEE43 pKa = 4.43 RR44 pKa = 11.84 MPGVAAHH51 pKa = 6.93 LKK53 pKa = 10.62 ACGPCQGDD61 pKa = 3.77 FTSLLDD67 pKa = 3.87 AVTDD71 pKa = 4.0 TPHH74 pKa = 6.39
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.884
IPC_protein 3.821
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A563E7U0|A0A563E7U0_9MICO Uncharacterized protein OS=Leekyejoonella antrihumi OX=1660198 GN=FGL98_02375 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLAGRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.88 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4772
0
4772
1527100
27
1888
320.0
34.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.492 ± 0.043
0.876 ± 0.009
6.086 ± 0.031
4.992 ± 0.037
2.802 ± 0.022
9.047 ± 0.03
2.523 ± 0.016
4.101 ± 0.025
2.124 ± 0.027
10.053 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.064 ± 0.014
1.967 ± 0.019
5.515 ± 0.025
3.351 ± 0.019
7.544 ± 0.04
5.828 ± 0.026
6.254 ± 0.032
8.775 ± 0.031
1.55 ± 0.015
2.057 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here