Salvia hispanica RNA virus 1
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386RWS8|A0A386RWS8_9VIRU Coat protein OS=Salvia hispanica RNA virus 1 OX=2419805 PE=4 SV=1
MM1 pKa = 7.26 AAEE4 pKa = 4.23 APRR7 pKa = 11.84 RR8 pKa = 11.84 VLAVKK13 pKa = 8.75 TPPNYY18 pKa = 10.81 DD19 pKa = 3.25 EE20 pKa = 4.96 MLTNTLAPLAAEE32 pKa = 4.86 AFPVHH37 pKa = 5.42 TWTRR41 pKa = 11.84 AALLANYY48 pKa = 10.23 LDD50 pKa = 3.19 IKK52 pKa = 10.87 RR53 pKa = 11.84 FIDD56 pKa = 3.45 HH57 pKa = 6.32 VKK59 pKa = 10.56 VISGEE64 pKa = 3.83 NDD66 pKa = 3.24 PVIRR70 pKa = 11.84 TAMIRR75 pKa = 11.84 KK76 pKa = 9.24 GNTSAAWNTQGTCTASQMFKK96 pKa = 10.09 FCAWLKK102 pKa = 10.57 SPEE105 pKa = 4.07 GNKK108 pKa = 10.22 FITTEE113 pKa = 3.47 RR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 10.59 RR117 pKa = 11.84 NIEE120 pKa = 3.48 KK121 pKa = 10.38 RR122 pKa = 11.84 AFEE125 pKa = 4.27 GQQFTDD131 pKa = 3.35 SSANAAMEE139 pKa = 4.05 AQITEE144 pKa = 3.87 HH145 pKa = 6.6 AMLVKK150 pKa = 10.58 KK151 pKa = 10.66 SRR153 pKa = 11.84 ATTEE157 pKa = 3.73 EE158 pKa = 3.85 TLLQLRR164 pKa = 11.84 RR165 pKa = 11.84 EE166 pKa = 4.26 IARR169 pKa = 11.84 TQQRR173 pKa = 11.84 GEE175 pKa = 3.82 ADD177 pKa = 3.26 IKK179 pKa = 11.24 ALEE182 pKa = 4.3 ADD184 pKa = 4.72 FAPGSAYY191 pKa = 10.79 VPMDD195 pKa = 3.98 DD196 pKa = 4.01 QDD198 pKa = 3.91 LGRR201 pKa = 11.84 ACHH204 pKa = 5.85 QLYY207 pKa = 10.14 LAQCQRR213 pKa = 11.84 EE214 pKa = 4.17 DD215 pKa = 4.5 ADD217 pKa = 4.43 PEE219 pKa = 4.33 DD220 pKa = 3.81 LTEE223 pKa = 5.74 EE224 pKa = 3.69 IMADD228 pKa = 3.11 IKK230 pKa = 11.03 ATFGSEE236 pKa = 2.94 ATARR240 pKa = 11.84 HH241 pKa = 5.49 RR242 pKa = 11.84 AAFIAEE248 pKa = 3.83 GHH250 pKa = 6.09 RR251 pKa = 11.84 RR252 pKa = 11.84 QDD254 pKa = 3.4 LLAWTEE260 pKa = 3.9 AKK262 pKa = 10.12 VQQLAGLGDD271 pKa = 4.53 LKK273 pKa = 10.93 RR274 pKa = 11.84 ADD276 pKa = 3.64 TFRR279 pKa = 11.84 VLVEE283 pKa = 4.09 GMGGEE288 pKa = 4.75 LSAQEE293 pKa = 3.78 RR294 pKa = 11.84 AAAEE298 pKa = 4.03 ARR300 pKa = 11.84 AVAAGGLGPNRR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 RR314 pKa = 11.84 RR315 pKa = 11.84 ADD317 pKa = 3.07 VDD319 pKa = 3.39 PQNIIDD325 pKa = 3.62 HH326 pKa = 5.93 PRR328 pKa = 11.84 RR329 pKa = 11.84 RR330 pKa = 11.84 GNQASVLQVEE340 pKa = 5.07 APPPPVVQGGDD351 pKa = 3.0 AGSRR355 pKa = 11.84 AGSPSNPPTDD365 pKa = 3.07 IRR367 pKa = 11.84 IASPRR372 pKa = 11.84 NEE374 pKa = 4.27 GGGEE378 pKa = 3.96 NPDD381 pKa = 3.36 VQEE384 pKa = 4.01
Molecular weight: 42.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.2
IPC2_protein 6.097
IPC_protein 6.173
Toseland 6.478
ProMoST 6.326
Dawson 6.453
Bjellqvist 6.44
Wikipedia 6.465
Rodwell 6.44
Grimsley 6.605
Solomon 6.465
Lehninger 6.453
Nozaki 6.707
DTASelect 6.898
Thurlkill 6.912
EMBOSS 6.883
Sillero 6.825
Patrickios 4.838
IPC_peptide 6.478
IPC2_peptide 6.722
IPC2.peptide.svr19 6.738
Protein with the highest isoelectric point:
>tr|A0A386RWS8|A0A386RWS8_9VIRU Coat protein OS=Salvia hispanica RNA virus 1 OX=2419805 PE=4 SV=1
MM1 pKa = 7.26 AAEE4 pKa = 4.23 APRR7 pKa = 11.84 RR8 pKa = 11.84 VLAVKK13 pKa = 8.75 TPPNYY18 pKa = 10.81 DD19 pKa = 3.25 EE20 pKa = 4.96 MLTNTLAPLAAEE32 pKa = 4.86 AFPVHH37 pKa = 5.42 TWTRR41 pKa = 11.84 AALLANYY48 pKa = 10.23 LDD50 pKa = 3.19 IKK52 pKa = 10.87 RR53 pKa = 11.84 FIDD56 pKa = 3.45 HH57 pKa = 6.32 VKK59 pKa = 10.56 VISGEE64 pKa = 3.83 NDD66 pKa = 3.24 PVIRR70 pKa = 11.84 TAMIRR75 pKa = 11.84 KK76 pKa = 9.24 GNTSAAWNTQGTCTASQMFKK96 pKa = 10.09 FCAWLKK102 pKa = 10.57 SPEE105 pKa = 4.07 GNKK108 pKa = 10.22 FITTEE113 pKa = 3.47 RR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 10.59 RR117 pKa = 11.84 NIEE120 pKa = 3.48 KK121 pKa = 10.38 RR122 pKa = 11.84 AFEE125 pKa = 4.27 GQQFTDD131 pKa = 3.35 SSANAAMEE139 pKa = 4.05 AQITEE144 pKa = 3.87 HH145 pKa = 6.6 AMLVKK150 pKa = 10.58 KK151 pKa = 10.66 SRR153 pKa = 11.84 ATTEE157 pKa = 3.73 EE158 pKa = 3.85 TLLQLRR164 pKa = 11.84 RR165 pKa = 11.84 EE166 pKa = 4.26 IARR169 pKa = 11.84 TQQRR173 pKa = 11.84 GEE175 pKa = 3.82 ADD177 pKa = 3.26 IKK179 pKa = 11.24 ALEE182 pKa = 4.3 ADD184 pKa = 4.72 FAPGSAYY191 pKa = 10.79 VPMDD195 pKa = 3.98 DD196 pKa = 4.01 QDD198 pKa = 3.91 LGRR201 pKa = 11.84 ACHH204 pKa = 5.85 QLYY207 pKa = 10.14 LAQCQRR213 pKa = 11.84 EE214 pKa = 4.17 DD215 pKa = 4.5 ADD217 pKa = 4.43 PEE219 pKa = 4.33 DD220 pKa = 3.81 LTEE223 pKa = 5.74 EE224 pKa = 3.69 IMADD228 pKa = 3.11 IKK230 pKa = 11.03 ATFGSEE236 pKa = 2.94 ATARR240 pKa = 11.84 HH241 pKa = 5.49 RR242 pKa = 11.84 AAFIAEE248 pKa = 3.83 GHH250 pKa = 6.09 RR251 pKa = 11.84 RR252 pKa = 11.84 QDD254 pKa = 3.4 LLAWTEE260 pKa = 3.9 AKK262 pKa = 10.12 VQQLAGLGDD271 pKa = 4.53 LKK273 pKa = 11.02 RR274 pKa = 11.84 ADD276 pKa = 3.77 TFVSSWKK283 pKa = 9.5 EE284 pKa = 3.48 WAEE287 pKa = 3.92 SFLLKK292 pKa = 10.37 RR293 pKa = 11.84 EE294 pKa = 3.77 PRR296 pKa = 11.84 LRR298 pKa = 11.84 RR299 pKa = 11.84 EE300 pKa = 4.12 LLQQVVWGPIAAGGGLTSTHH320 pKa = 7.14 RR321 pKa = 11.84 ISSIIPADD329 pKa = 3.74 VVTKK333 pKa = 7.78 PQCSKK338 pKa = 10.74 SKK340 pKa = 10.24 RR341 pKa = 11.84 RR342 pKa = 11.84 PPLSSKK348 pKa = 10.89 VEE350 pKa = 4.16 TLDD353 pKa = 3.78 LAPGLLVTHH362 pKa = 6.05 QRR364 pKa = 11.84 TSVLRR369 pKa = 11.84 VPAMKK374 pKa = 10.52 GGGRR378 pKa = 11.84 IPTSRR383 pKa = 11.84 SKK385 pKa = 11.24 FEE387 pKa = 3.94 AKK389 pKa = 9.08 VRR391 pKa = 11.84 RR392 pKa = 11.84 VIGGGEE398 pKa = 3.7 MRR400 pKa = 11.84 NWAKK404 pKa = 10.74 DD405 pKa = 3.29 SNMYY409 pKa = 10.39 RR410 pKa = 11.84 GGGNYY415 pKa = 9.86 SDD417 pKa = 4.29 ALKK420 pKa = 10.95 LLATARR426 pKa = 11.84 TDD428 pKa = 3.09 IPGSFLHH435 pKa = 6.77 KK436 pKa = 10.32 HH437 pKa = 4.58 FTVNNARR444 pKa = 11.84 SFLKK448 pKa = 10.41 LPCGLPVPVGPEE460 pKa = 3.83 SVRR463 pKa = 11.84 MKK465 pKa = 10.82 NFNEE469 pKa = 4.09 EE470 pKa = 3.78 ATAGPSFRR478 pKa = 11.84 AFGIYY483 pKa = 9.49 RR484 pKa = 11.84 KK485 pKa = 9.9 KK486 pKa = 10.53 GLKK489 pKa = 10.11 AEE491 pKa = 4.48 LEE493 pKa = 4.16 ATVWKK498 pKa = 10.29 SLHH501 pKa = 6.57 AYY503 pKa = 10.31 AEE505 pKa = 4.55 GGDD508 pKa = 4.21 AEE510 pKa = 4.49 SCLPFIAARR519 pKa = 11.84 VGYY522 pKa = 9.64 RR523 pKa = 11.84 SKK525 pKa = 11.42 LLTLEE530 pKa = 4.19 KK531 pKa = 10.56 AFSKK535 pKa = 9.52 LTSGEE540 pKa = 4.11 CLGRR544 pKa = 11.84 CVMMLDD550 pKa = 4.28 AFEE553 pKa = 4.2 QTYY556 pKa = 11.16 SSALYY561 pKa = 10.28 NVISGITHH569 pKa = 7.21 RR570 pKa = 11.84 GRR572 pKa = 11.84 HH573 pKa = 5.11 NPGSSFRR580 pKa = 11.84 NTTVRR585 pKa = 11.84 ASSDD589 pKa = 2.37 WGMLFEE595 pKa = 4.36 EE596 pKa = 4.79 VKK598 pKa = 10.53 KK599 pKa = 10.82 ASAVIEE605 pKa = 4.2 LDD607 pKa = 2.83 WKK609 pKa = 11.04 KK610 pKa = 10.65 FDD612 pKa = 4.63 RR613 pKa = 11.84 DD614 pKa = 3.58 RR615 pKa = 11.84 PSDD618 pKa = 4.27 DD619 pKa = 3.18 IYY621 pKa = 11.37 FVIDD625 pKa = 4.45 IILSCFQPKK634 pKa = 9.97 NGYY637 pKa = 7.39 EE638 pKa = 3.82 ARR640 pKa = 11.84 LLEE643 pKa = 3.9 AHH645 pKa = 7.23 GIMLRR650 pKa = 11.84 RR651 pKa = 11.84 ALVEE655 pKa = 4.2 RR656 pKa = 11.84 PFITDD661 pKa = 3.19 DD662 pKa = 3.41 GGIFTIEE669 pKa = 3.92 GMVPSGSLWTGWLDD683 pKa = 3.45 TALNTLYY690 pKa = 10.31 IKK692 pKa = 10.71 AVLFHH697 pKa = 6.76 LGFRR701 pKa = 11.84 EE702 pKa = 3.93 SDD704 pKa = 3.46 ASPKK708 pKa = 10.43 CAGDD712 pKa = 4.07 DD713 pKa = 3.55 NLTLIFKK720 pKa = 9.43 EE721 pKa = 4.1 ATDD724 pKa = 3.75 MQLMEE729 pKa = 4.72 VKK731 pKa = 10.43 RR732 pKa = 11.84 LLNEE736 pKa = 4.15 WFRR739 pKa = 11.84 AGIDD743 pKa = 3.89 DD744 pKa = 4.2 EE745 pKa = 5.68 DD746 pKa = 5.36 FMVHH750 pKa = 6.31 HH751 pKa = 7.19 PPYY754 pKa = 10.05 HH755 pKa = 5.67 VEE757 pKa = 3.55 RR758 pKa = 11.84 RR759 pKa = 11.84 QAVFPPGTDD768 pKa = 3.28 LSKK771 pKa = 10.65 GTSKK775 pKa = 11.04 LLDD778 pKa = 3.68 LAQWVPFEE786 pKa = 5.12 DD787 pKa = 4.24 PMTIDD792 pKa = 3.27 EE793 pKa = 4.82 SKK795 pKa = 10.96 GLSHH799 pKa = 6.55 RR800 pKa = 11.84 WKK802 pKa = 10.84 YY803 pKa = 10.52 IFEE806 pKa = 4.46 NKK808 pKa = 9.54 PKK810 pKa = 10.38 FLSCYY815 pKa = 8.45 WDD817 pKa = 3.59 EE818 pKa = 4.44 NNNPIRR824 pKa = 11.84 PAYY827 pKa = 9.63 INLEE831 pKa = 3.91 KK832 pKa = 10.85 LLWPEE837 pKa = 5.22 GIHH840 pKa = 5.58 EE841 pKa = 4.5 TIEE844 pKa = 4.41 DD845 pKa = 3.93 YY846 pKa = 10.87 EE847 pKa = 4.18 AALIGMVVDD856 pKa = 4.66 NPFNHH861 pKa = 6.71 HH862 pKa = 5.73 NVNHH866 pKa = 6.29 LMHH869 pKa = 7.4 RR870 pKa = 11.84 YY871 pKa = 10.01 CIVQQVKK878 pKa = 10.09 RR879 pKa = 11.84 MMITGIKK886 pKa = 10.32 AEE888 pKa = 4.58 DD889 pKa = 3.45 VLDD892 pKa = 4.15 FCRR895 pKa = 11.84 FKK897 pKa = 10.99 DD898 pKa = 3.82 DD899 pKa = 3.82 KK900 pKa = 11.74 GEE902 pKa = 4.15 GVPFPMVAQWRR913 pKa = 11.84 RR914 pKa = 11.84 VNGWVDD920 pKa = 3.39 MEE922 pKa = 4.62 KK923 pKa = 10.88 LPFVDD928 pKa = 5.42 RR929 pKa = 11.84 YY930 pKa = 9.53 IAQFRR935 pKa = 11.84 DD936 pKa = 3.8 FVTGVTSLYY945 pKa = 10.58 SRR947 pKa = 11.84 SPTGGLDD954 pKa = 2.6 SWRR957 pKa = 11.84 FMDD960 pKa = 5.26 IIRR963 pKa = 11.84 GVGNLNDD970 pKa = 3.69 AQFGNDD976 pKa = 2.84 MVDD979 pKa = 2.16 WVTFLKK985 pKa = 10.56 GHH987 pKa = 7.31 PLTKK991 pKa = 10.16 YY992 pKa = 10.1 LKK994 pKa = 7.9 PTKK997 pKa = 10.16 GSRR1000 pKa = 11.84 KK1001 pKa = 9.32 RR1002 pKa = 11.84 KK1003 pKa = 8.52 LHH1005 pKa = 6.36 SDD1007 pKa = 3.64 PEE1009 pKa = 4.36 EE1010 pKa = 3.9 EE1011 pKa = 3.76 ALTRR1015 pKa = 11.84 FKK1017 pKa = 11.2 VLNHH1021 pKa = 6.07 YY1022 pKa = 10.52 LDD1024 pKa = 4.08 PTWKK1028 pKa = 10.42 KK1029 pKa = 11.38 KK1030 pKa = 10.17 MFDD1033 pKa = 4.05 DD1034 pKa = 3.81 VDD1036 pKa = 5.22 SYY1038 pKa = 12.15 ALWISDD1044 pKa = 3.69 LLRR1047 pKa = 11.84 NKK1049 pKa = 10.47 GPTSS1053 pKa = 3.34
Molecular weight: 119.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.518
IPC2_protein 7.892
IPC_protein 7.717
Toseland 7.629
ProMoST 8.317
Dawson 8.551
Bjellqvist 8.756
Wikipedia 8.507
Rodwell 8.609
Grimsley 7.688
Solomon 8.609
Lehninger 8.609
Nozaki 8.946
DTASelect 8.536
Thurlkill 8.639
EMBOSS 8.741
Sillero 8.902
Patrickios 4.774
IPC_peptide 8.595
IPC2_peptide 7.512
IPC2.peptide.svr19 7.757
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1437
384
1053
718.5
80.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.299 ± 2.001
1.253 ± 0.093
6.193 ± 0.09
6.75 ± 0.468
4.245 ± 0.608
6.611 ± 0.07
2.366 ± 0.239
4.662 ± 0.104
5.776 ± 0.823
8.49 ± 0.757
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.714 ± 0.163
3.688 ± 0.096
5.15 ± 0.255
3.479 ± 0.876
7.794 ± 0.696
5.289 ± 0.609
5.985 ± 0.117
5.289 ± 0.265
1.949 ± 0.399
2.018 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here