Streptococcus phage Javan444
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6B490|A0A4D6B490_9CAUD Antirepressor protein OS=Streptococcus phage Javan444 OX=2548170 GN=Javan444_0048 PE=4 SV=1
MM1 pKa = 8.07 EE2 pKa = 5.02 IRR4 pKa = 11.84 QVSDD8 pKa = 3.04 SVAIYY13 pKa = 10.84 SDD15 pKa = 3.68 GQRR18 pKa = 11.84 LQVIHH23 pKa = 6.56 NLGDD27 pKa = 3.62 EE28 pKa = 5.11 FILDD32 pKa = 3.57 FDD34 pKa = 4.21 FQIEE38 pKa = 4.31 EE39 pKa = 4.27 VVNIDD44 pKa = 4.18 DD45 pKa = 4.06 LTTHH49 pKa = 5.46 VVGDD53 pKa = 3.7 IVPIFKK59 pKa = 10.8 VSGYY63 pKa = 9.37 CSRR66 pKa = 11.84 SGEE69 pKa = 4.07 DD70 pKa = 3.13 MYY72 pKa = 11.32 RR73 pKa = 11.84 LKK75 pKa = 10.36 WAIRR79 pKa = 11.84 QFEE82 pKa = 4.23 IFEE85 pKa = 4.36 QYY87 pKa = 11.21 LNDD90 pKa = 3.72 YY91 pKa = 8.1 QTEE94 pKa = 4.14 LVEE97 pKa = 4.05 WLGNLGGEE105 pKa = 4.5 DD106 pKa = 3.45 VV107 pKa = 3.98
Molecular weight: 12.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.103
IPC2_protein 4.05
IPC_protein 3.999
Toseland 3.795
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.884
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.91
Nozaki 4.088
DTASelect 4.279
Thurlkill 3.846
EMBOSS 3.897
Sillero 4.113
Patrickios 3.198
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.016
Protein with the highest isoelectric point:
>tr|A0A4D6B7C7|A0A4D6B7C7_9CAUD Terminase large subunit OS=Streptococcus phage Javan444 OX=2548170 GN=Javan444_0025 PE=4 SV=1
MM1 pKa = 7.41 GATVSVKK8 pKa = 10.16 VDD10 pKa = 3.35 LKK12 pKa = 11.18 GIEE15 pKa = 4.36 KK16 pKa = 10.08 KK17 pKa = 10.87 VSSAALAKK25 pKa = 10.35 GKK27 pKa = 9.93 LAIASQMKK35 pKa = 9.59 MDD37 pKa = 4.22 MTPFIPRR44 pKa = 11.84 KK45 pKa = 9.65 SGEE48 pKa = 4.14 LSGSAQVSKK57 pKa = 11.26 DD58 pKa = 3.22 GIRR61 pKa = 11.84 YY62 pKa = 6.35 PGPYY66 pKa = 10.0 ARR68 pKa = 11.84 AQFYY72 pKa = 10.28 GSSYY76 pKa = 10.43 NKK78 pKa = 9.74 YY79 pKa = 10.36 RR80 pKa = 11.84 SFTYY84 pKa = 10.12 KK85 pKa = 10.47 RR86 pKa = 11.84 YY87 pKa = 5.44 TTPGTGKK94 pKa = 10.27 RR95 pKa = 11.84 WDD97 pKa = 3.89 LKK99 pKa = 11.18 ASALHH104 pKa = 5.99 IKK106 pKa = 10.19 DD107 pKa = 2.95 WGKK110 pKa = 10.65 VGLRR114 pKa = 11.84 AMGVKK119 pKa = 10.0 AA120 pKa = 4.47
Molecular weight: 13.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.838
IPC_protein 9.955
Toseland 10.511
ProMoST 10.087
Dawson 10.643
Bjellqvist 10.262
Wikipedia 10.774
Rodwell 11.257
Grimsley 10.687
Solomon 10.657
Lehninger 10.643
Nozaki 10.467
DTASelect 10.262
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.54
Patrickios 10.979
IPC_peptide 10.672
IPC2_peptide 8.799
IPC2.peptide.svr19 8.715
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
11566
43
1191
222.4
24.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.643 ± 0.854
0.372 ± 0.082
6.173 ± 0.439
6.865 ± 0.677
3.804 ± 0.194
7.219 ± 0.561
1.21 ± 0.153
6.64 ± 0.237
7.92 ± 0.576
7.807 ± 0.392
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.585 ± 0.221
5.732 ± 0.411
2.827 ± 0.156
4.116 ± 0.257
3.925 ± 0.258
6.934 ± 0.604
6.536 ± 0.441
6.389 ± 0.377
1.254 ± 0.144
4.046 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here