Rice tungro bacilliform virus (isolate Philippines) (RTBV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Tungrovirus; Rice tungro bacilliform virus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P27502|POL_RTBVP Polyprotein P3 OS=Rice tungro bacilliform virus (isolate Philippines) OX=10655 PE=1 SV=1
MM1 pKa = 7.34NIEE4 pKa = 4.18YY5 pKa = 8.91PYY7 pKa = 10.17SIHH10 pKa = 8.09IIDD13 pKa = 4.04KK14 pKa = 10.13NKK16 pKa = 9.96VPIYY20 pKa = 10.09DD21 pKa = 3.47QGNLFHH27 pKa = 6.52TEE29 pKa = 3.4KK30 pKa = 10.56SSRR33 pKa = 11.84LSHH36 pKa = 6.28VSRR39 pKa = 11.84GLLDD43 pKa = 3.45HH44 pKa = 7.03LFTFSSDD51 pKa = 2.72NTEE54 pKa = 3.94RR55 pKa = 11.84VRR57 pKa = 11.84KK58 pKa = 9.12LHH60 pKa = 6.43ILADD64 pKa = 3.74YY65 pKa = 10.82LYY67 pKa = 9.83LLEE70 pKa = 4.98SEE72 pKa = 4.29RR73 pKa = 11.84EE74 pKa = 4.14SYY76 pKa = 10.09KK77 pKa = 10.97NEE79 pKa = 3.94WISLKK84 pKa = 10.84DD85 pKa = 3.63QVSLLQKK92 pKa = 10.48QNSEE96 pKa = 3.56LRR98 pKa = 11.84ARR100 pKa = 11.84IATNKK105 pKa = 10.05EE106 pKa = 3.64IIEE109 pKa = 4.15GLRR112 pKa = 11.84EE113 pKa = 3.97PVKK116 pKa = 10.83KK117 pKa = 10.14PIYY120 pKa = 6.32TTQDD124 pKa = 2.89KK125 pKa = 10.68EE126 pKa = 3.92RR127 pKa = 11.84LRR129 pKa = 11.84VFFCEE134 pKa = 4.12EE135 pKa = 3.35RR136 pKa = 11.84SMEE139 pKa = 4.06YY140 pKa = 9.57IYY142 pKa = 11.1YY143 pKa = 9.86HH144 pKa = 6.91IKK146 pKa = 10.55RR147 pKa = 11.84LAQQSYY153 pKa = 10.0YY154 pKa = 11.22SHH156 pKa = 7.29LNNLQKK162 pKa = 10.8DD163 pKa = 4.07CEE165 pKa = 4.34PFRR168 pKa = 11.84GVYY171 pKa = 9.44MSFLTNVKK179 pKa = 10.18FLVLCEE185 pKa = 3.89AGYY188 pKa = 8.13WTVPDD193 pKa = 3.8IEE195 pKa = 4.8TNTTEE200 pKa = 4.7SILSLSQKK208 pKa = 10.29KK209 pKa = 10.51GEE211 pKa = 4.48DD212 pKa = 3.48LLQKK216 pKa = 10.69GVVIFNEE223 pKa = 4.23LEE225 pKa = 3.86GGYY228 pKa = 9.33QLSPRR233 pKa = 11.84FIGDD237 pKa = 3.93LYY239 pKa = 11.01AHH241 pKa = 6.92GFIKK245 pKa = 10.42QINFTTKK252 pKa = 9.93VPEE255 pKa = 4.18GLPPIIAEE263 pKa = 4.21KK264 pKa = 10.02LQDD267 pKa = 3.65YY268 pKa = 9.46KK269 pKa = 11.37FPGSNTVLIEE279 pKa = 4.08RR280 pKa = 11.84EE281 pKa = 3.73IPRR284 pKa = 11.84WNFNEE289 pKa = 4.38MKK291 pKa = 10.28RR292 pKa = 11.84EE293 pKa = 3.88TQMRR297 pKa = 11.84TNLYY301 pKa = 8.54IFKK304 pKa = 10.31NYY306 pKa = 9.86RR307 pKa = 11.84CFYY310 pKa = 9.51GYY312 pKa = 10.71SPLRR316 pKa = 11.84PYY318 pKa = 10.91EE319 pKa = 4.46PITPEE324 pKa = 3.74EE325 pKa = 4.15FGFDD329 pKa = 3.61YY330 pKa = 11.02YY331 pKa = 11.27SWEE334 pKa = 3.98NMVDD338 pKa = 3.45EE339 pKa = 5.32DD340 pKa = 4.04EE341 pKa = 5.46GEE343 pKa = 4.06VVYY346 pKa = 10.58ISKK349 pKa = 8.2YY350 pKa = 7.64TKK352 pKa = 9.64IIKK355 pKa = 7.73VTKK358 pKa = 7.54EE359 pKa = 3.94HH360 pKa = 5.6AWAWPEE366 pKa = 3.77HH367 pKa = 6.98DD368 pKa = 5.06GDD370 pKa = 4.27TMSCTTSIEE379 pKa = 4.24DD380 pKa = 3.47EE381 pKa = 4.55WIHH384 pKa = 7.91RR385 pKa = 11.84MDD387 pKa = 3.61NAA389 pKa = 4.25

Molecular weight: 46.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P27500|P1_RTBVP Protein P1 OS=Rice tungro bacilliform virus (isolate Philippines) OX=10655 PE=4 SV=3
MM1 pKa = 7.17SADD4 pKa = 3.3YY5 pKa = 8.21PTFKK9 pKa = 10.54EE10 pKa = 3.97ALEE13 pKa = 4.08KK14 pKa = 10.77FKK16 pKa = 11.18NLEE19 pKa = 3.71SDD21 pKa = 3.44TAGKK25 pKa = 10.42DD26 pKa = 2.97KK27 pKa = 10.96FNWVFTLEE35 pKa = 4.38NIKK38 pKa = 10.61SAADD42 pKa = 3.19VNLASKK48 pKa = 10.95GLVQLYY54 pKa = 10.22ALQEE58 pKa = 3.72IDD60 pKa = 4.56KK61 pKa = 10.49KK62 pKa = 11.09INNLTTQVSKK72 pKa = 11.28LPTTSGSSSAGAIVPAGSNTQGQYY96 pKa = 10.41KK97 pKa = 10.25APPKK101 pKa = 10.32KK102 pKa = 10.34GIKK105 pKa = 9.46RR106 pKa = 11.84KK107 pKa = 10.13YY108 pKa = 8.2PAA110 pKa = 4.41

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2373

110

1675

593.3

68.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.425 ± 1.059

1.391 ± 0.392

5.942 ± 0.514

9.313 ± 0.812

3.961 ± 0.221

4.256 ± 0.85

2.023 ± 0.379

8.386 ± 0.763

10.619 ± 1.651

8.091 ± 1.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.812 ± 0.523

5.9 ± 0.272

3.456 ± 0.547

3.751 ± 0.754

4.34 ± 0.684

6.49 ± 0.611

5.9 ± 0.207

4.214 ± 0.199

1.264 ± 0.255

4.467 ± 0.781
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski