Moraxella phage Mcat20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R6PJC6|A0A0R6PJC6_9CAUD Uncharacterized protein OS=Moraxella phage Mcat20 OX=1647536 PE=4 SV=1
MM1 pKa = 7.26SASEE5 pKa = 3.94LSEE8 pKa = 3.38WLAYY12 pKa = 10.31DD13 pKa = 3.55RR14 pKa = 11.84LDD16 pKa = 4.36PIGGYY21 pKa = 10.06RR22 pKa = 11.84GDD24 pKa = 3.52IQAATIAASMGGGKK38 pKa = 10.32LSDD41 pKa = 3.84YY42 pKa = 10.47MIIDD46 pKa = 4.62PNPMTDD52 pKa = 3.19EE53 pKa = 3.76MRR55 pKa = 11.84EE56 pKa = 3.91EE57 pKa = 3.88YY58 pKa = 10.3EE59 pKa = 4.1YY60 pKa = 10.82YY61 pKa = 10.2QRR63 pKa = 11.84LAEE66 pKa = 5.02LEE68 pKa = 4.43DD69 pKa = 3.86EE70 pKa = 4.18QSLLIEE76 pKa = 4.6RR77 pKa = 11.84FNHH80 pKa = 6.79LEE82 pKa = 4.01DD83 pKa = 3.74VQQ85 pKa = 3.68

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R6PIW7|A0A0R6PIW7_9CAUD Portal protein OS=Moraxella phage Mcat20 OX=1647536 PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 10.48VSVKK6 pKa = 10.34IEE8 pKa = 3.9GLKK11 pKa = 10.69EE12 pKa = 3.69LDD14 pKa = 3.64EE15 pKa = 4.12QLGKK19 pKa = 9.65IDD21 pKa = 3.55KK22 pKa = 9.69EE23 pKa = 4.12FRR25 pKa = 11.84GKK27 pKa = 10.07SLYY30 pKa = 10.37EE31 pKa = 3.95SLNFASQPMLNAARR45 pKa = 11.84RR46 pKa = 11.84GARR49 pKa = 11.84VAEE52 pKa = 4.21KK53 pKa = 10.12RR54 pKa = 11.84YY55 pKa = 9.18RR56 pKa = 11.84WYY58 pKa = 9.76MSHH61 pKa = 6.18GQGEE65 pKa = 4.36ATYY68 pKa = 11.15VKK70 pKa = 9.44TKK72 pKa = 9.15NGKK75 pKa = 7.56WRR77 pKa = 11.84AGKK80 pKa = 9.56SKK82 pKa = 10.08RR83 pKa = 11.84AKK85 pKa = 9.61RR86 pKa = 11.84GEE88 pKa = 4.3GKK90 pKa = 10.53FEE92 pKa = 3.85WQEE95 pKa = 3.59PGLLKK100 pKa = 10.65KK101 pKa = 10.7AFADD105 pKa = 3.33AGSIKK110 pKa = 10.07KK111 pKa = 10.12VKK113 pKa = 10.32ISIEE117 pKa = 3.94PLSVSICTKK126 pKa = 8.68AQEE129 pKa = 4.13RR130 pKa = 11.84RR131 pKa = 11.84TDD133 pKa = 3.32QLIIGGLLSMVLKK146 pKa = 10.63KK147 pKa = 10.52CLPRR151 pKa = 11.84LLSVQLIIIMKK162 pKa = 9.58LKK164 pKa = 10.65RR165 pKa = 11.84LIDD168 pKa = 3.84LNRR171 pKa = 11.84AA172 pKa = 3.31

Molecular weight: 19.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

7183

37

1460

199.5

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.952 ± 0.935

1.058 ± 0.274

6.153 ± 0.334

6.112 ± 0.257

3.55 ± 0.182

6.557 ± 0.333

1.991 ± 0.223

5.931 ± 0.268

7.921 ± 0.401

8.952 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.198

5.402 ± 0.291

3.105 ± 0.435

4.274 ± 0.237

4.622 ± 0.257

6.39 ± 0.323

5.931 ± 0.32

5.694 ± 0.232

1.309 ± 0.209

3.327 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski