Amino acid dipepetide frequency for Moraxella phage Mcat20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.569AlaAla: 5.569 ± 1.166
1.392AlaCys: 1.392 ± 0.528
5.152AlaAsp: 5.152 ± 0.645
5.709AlaGlu: 5.709 ± 1.09
2.646AlaPhe: 2.646 ± 0.691
5.569AlaGly: 5.569 ± 0.873
1.949AlaHis: 1.949 ± 0.448
4.177AlaIle: 4.177 ± 0.67
7.38AlaLys: 7.38 ± 1.499
10.164AlaLeu: 10.164 ± 1.764
1.949AlaMet: 1.949 ± 0.634
5.152AlaAsn: 5.152 ± 0.798
1.532AlaPro: 1.532 ± 0.335
4.734AlaGln: 4.734 ± 0.872
4.038AlaArg: 4.038 ± 0.543
7.101AlaSer: 7.101 ± 0.88
7.24AlaThr: 7.24 ± 1.167
5.43AlaVal: 5.43 ± 0.802
0.696AlaTrp: 0.696 ± 0.376
1.949AlaTyr: 1.949 ± 0.636
0.0AlaXaa: 0.0 ± 0.0
Cys
0.278CysAla: 0.278 ± 0.229
0.139CysCys: 0.139 ± 0.115
0.696CysAsp: 0.696 ± 0.333
0.418CysGlu: 0.418 ± 0.251
0.278CysPhe: 0.278 ± 0.211
0.557CysGly: 0.557 ± 0.484
0.418CysHis: 0.418 ± 0.235
0.557CysIle: 0.557 ± 0.465
1.253CysLys: 1.253 ± 0.536
1.392CysLeu: 1.392 ± 0.479
0.278CysMet: 0.278 ± 0.231
0.557CysAsn: 0.557 ± 0.307
0.418CysPro: 0.418 ± 0.252
0.557CysGln: 0.557 ± 0.257
0.975CysArg: 0.975 ± 0.308
0.975CysSer: 0.975 ± 0.416
0.278CysThr: 0.278 ± 0.176
0.557CysVal: 0.557 ± 0.269
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.873AspAla: 4.873 ± 0.822
0.696AspCys: 0.696 ± 0.332
5.152AspAsp: 5.152 ± 1.227
5.709AspGlu: 5.709 ± 0.817
3.202AspPhe: 3.202 ± 0.715
6.405AspGly: 6.405 ± 0.872
0.975AspHis: 0.975 ± 0.396
4.038AspIle: 4.038 ± 0.608
4.038AspLys: 4.038 ± 0.705
4.038AspLeu: 4.038 ± 0.639
1.532AspMet: 1.532 ± 0.361
4.595AspAsn: 4.595 ± 0.882
1.532AspPro: 1.532 ± 0.597
1.532AspGln: 1.532 ± 0.602
2.089AspArg: 2.089 ± 0.564
2.785AspSer: 2.785 ± 0.605
2.367AspThr: 2.367 ± 0.502
3.202AspVal: 3.202 ± 0.477
1.253AspTrp: 1.253 ± 0.425
1.532AspTyr: 1.532 ± 0.407
0.0AspXaa: 0.0 ± 0.0
Glu
3.481GluAla: 3.481 ± 0.568
0.696GluCys: 0.696 ± 0.382
3.063GluAsp: 3.063 ± 0.768
3.063GluGlu: 3.063 ± 0.723
3.899GluPhe: 3.899 ± 0.636
1.81GluGly: 1.81 ± 0.422
2.367GluHis: 2.367 ± 0.506
3.759GluIle: 3.759 ± 0.694
4.734GluLys: 4.734 ± 0.758
7.24GluLeu: 7.24 ± 0.934
1.392GluMet: 1.392 ± 0.373
3.62GluAsn: 3.62 ± 0.619
2.367GluPro: 2.367 ± 0.733
4.456GluGln: 4.456 ± 0.676
3.481GluArg: 3.481 ± 0.615
2.367GluSer: 2.367 ± 0.466
2.228GluThr: 2.228 ± 0.54
3.481GluVal: 3.481 ± 0.785
0.696GluTrp: 0.696 ± 0.334
2.924GluTyr: 2.924 ± 0.565
0.0GluXaa: 0.0 ± 0.0
Phe
3.899PheAla: 3.899 ± 0.879
0.557PheCys: 0.557 ± 0.294
3.342PheAsp: 3.342 ± 0.643
3.063PheGlu: 3.063 ± 0.823
0.696PhePhe: 0.696 ± 0.267
3.481PheGly: 3.481 ± 0.658
0.418PheHis: 0.418 ± 0.284
2.367PheIle: 2.367 ± 0.478
2.228PheLys: 2.228 ± 0.577
2.089PheLeu: 2.089 ± 0.504
0.418PheMet: 0.418 ± 0.285
1.671PheAsn: 1.671 ± 0.449
0.418PhePro: 0.418 ± 0.254
0.139PheGln: 0.139 ± 0.123
1.114PheArg: 1.114 ± 0.337
1.81PheSer: 1.81 ± 0.493
2.924PheThr: 2.924 ± 0.513
3.202PheVal: 3.202 ± 0.642
0.696PheTrp: 0.696 ± 0.383
0.975PheTyr: 0.975 ± 0.366
0.0PheXaa: 0.0 ± 0.0
Gly
5.43GlyAla: 5.43 ± 0.882
0.278GlyCys: 0.278 ± 0.23
4.038GlyAsp: 4.038 ± 0.702
4.316GlyGlu: 4.316 ± 0.792
3.202GlyPhe: 3.202 ± 0.555
6.544GlyGly: 6.544 ± 1.516
0.696GlyHis: 0.696 ± 0.363
4.316GlyIle: 4.316 ± 0.597
6.962GlyLys: 6.962 ± 0.989
5.709GlyLeu: 5.709 ± 0.846
2.089GlyMet: 2.089 ± 0.629
2.785GlyAsn: 2.785 ± 0.569
0.0GlyPro: 0.0 ± 0.0
1.81GlyGln: 1.81 ± 0.455
3.481GlyArg: 3.481 ± 0.576
3.62GlySer: 3.62 ± 0.629
3.481GlyThr: 3.481 ± 0.793
4.316GlyVal: 4.316 ± 0.935
0.835GlyTrp: 0.835 ± 0.311
1.671GlyTyr: 1.671 ± 0.64
0.0GlyXaa: 0.0 ± 0.0
His
1.81HisAla: 1.81 ± 0.457
0.139HisCys: 0.139 ± 0.147
1.253HisAsp: 1.253 ± 0.403
0.975HisGlu: 0.975 ± 0.295
0.696HisPhe: 0.696 ± 0.251
1.532HisGly: 1.532 ± 0.465
0.418HisHis: 0.418 ± 0.265
1.114HisIle: 1.114 ± 0.43
1.253HisLys: 1.253 ± 0.534
2.506HisLeu: 2.506 ± 0.562
0.557HisMet: 0.557 ± 0.305
1.532HisAsn: 1.532 ± 0.583
0.975HisPro: 0.975 ± 0.335
0.557HisGln: 0.557 ± 0.195
0.557HisArg: 0.557 ± 0.25
0.418HisSer: 0.418 ± 0.212
1.532HisThr: 1.532 ± 0.39
0.139HisVal: 0.139 ± 0.115
0.696HisTrp: 0.696 ± 0.37
1.253HisTyr: 1.253 ± 0.355
0.0HisXaa: 0.0 ± 0.0
Ile
4.873IleAla: 4.873 ± 0.609
0.557IleCys: 0.557 ± 0.261
4.873IleAsp: 4.873 ± 0.839
3.202IleGlu: 3.202 ± 0.521
1.392IlePhe: 1.392 ± 0.463
4.177IleGly: 4.177 ± 0.756
1.114IleHis: 1.114 ± 0.492
4.873IleIle: 4.873 ± 0.746
5.43IleLys: 5.43 ± 0.982
4.038IleLeu: 4.038 ± 0.478
1.81IleMet: 1.81 ± 0.526
3.481IleAsn: 3.481 ± 0.62
1.253IlePro: 1.253 ± 0.483
2.506IleGln: 2.506 ± 0.93
1.532IleArg: 1.532 ± 0.396
4.038IleSer: 4.038 ± 0.793
5.013IleThr: 5.013 ± 0.91
2.506IleVal: 2.506 ± 0.372
0.278IleTrp: 0.278 ± 0.18
2.367IleTyr: 2.367 ± 0.516
0.0IleXaa: 0.0 ± 0.0
Lys
9.468LysAla: 9.468 ± 1.823
0.418LysCys: 0.418 ± 0.26
4.316LysAsp: 4.316 ± 0.866
3.342LysGlu: 3.342 ± 0.801
2.924LysPhe: 2.924 ± 0.707
3.899LysGly: 3.899 ± 0.695
1.253LysHis: 1.253 ± 0.461
4.456LysIle: 4.456 ± 0.748
7.38LysLys: 7.38 ± 1.371
6.962LysLeu: 6.962 ± 0.754
2.646LysMet: 2.646 ± 0.568
4.595LysAsn: 4.595 ± 0.89
3.063LysPro: 3.063 ± 0.767
2.785LysGln: 2.785 ± 0.493
3.899LysArg: 3.899 ± 0.845
6.126LysSer: 6.126 ± 1.048
5.013LysThr: 5.013 ± 1.038
3.202LysVal: 3.202 ± 0.772
1.114LysTrp: 1.114 ± 0.419
2.367LysTyr: 2.367 ± 0.629
0.0LysXaa: 0.0 ± 0.0
Leu
8.911LeuAla: 8.911 ± 1.169
0.835LeuCys: 0.835 ± 0.523
6.405LeuAsp: 6.405 ± 0.794
5.013LeuGlu: 5.013 ± 0.905
2.646LeuPhe: 2.646 ± 0.496
4.177LeuGly: 4.177 ± 0.935
1.253LeuHis: 1.253 ± 0.472
5.152LeuIle: 5.152 ± 0.708
9.329LeuLys: 9.329 ± 0.942
6.126LeuLeu: 6.126 ± 1.043
2.089LeuMet: 2.089 ± 0.509
5.291LeuAsn: 5.291 ± 0.812
3.481LeuPro: 3.481 ± 0.786
3.063LeuGln: 3.063 ± 0.606
4.177LeuArg: 4.177 ± 0.564
6.823LeuSer: 6.823 ± 0.933
5.709LeuThr: 5.709 ± 1.004
5.152LeuVal: 5.152 ± 0.721
1.392LeuTrp: 1.392 ± 0.274
2.506LeuTyr: 2.506 ± 0.601
0.0LeuXaa: 0.0 ± 0.0
Met
2.228MetAla: 2.228 ± 0.58
0.139MetCys: 0.139 ± 0.146
1.392MetAsp: 1.392 ± 0.459
0.975MetGlu: 0.975 ± 0.457
0.975MetPhe: 0.975 ± 0.315
2.089MetGly: 2.089 ± 0.4
0.557MetHis: 0.557 ± 0.218
0.975MetIle: 0.975 ± 0.31
2.228MetLys: 2.228 ± 0.483
2.228MetLeu: 2.228 ± 0.452
0.975MetMet: 0.975 ± 0.398
1.392MetAsn: 1.392 ± 0.518
1.253MetPro: 1.253 ± 0.271
1.253MetGln: 1.253 ± 0.487
0.835MetArg: 0.835 ± 0.406
2.506MetSer: 2.506 ± 0.532
2.228MetThr: 2.228 ± 0.512
2.646MetVal: 2.646 ± 0.697
0.418MetTrp: 0.418 ± 0.2
0.418MetTyr: 0.418 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
5.152AsnAla: 5.152 ± 0.901
0.278AsnCys: 0.278 ± 0.211
2.506AsnAsp: 2.506 ± 0.805
3.342AsnGlu: 3.342 ± 0.703
2.367AsnPhe: 2.367 ± 0.563
3.202AsnGly: 3.202 ± 0.758
0.975AsnHis: 0.975 ± 0.432
3.481AsnIle: 3.481 ± 0.608
3.62AsnLys: 3.62 ± 0.708
5.013AsnLeu: 5.013 ± 0.831
2.367AsnMet: 2.367 ± 0.639
2.924AsnAsn: 2.924 ± 0.835
2.924AsnPro: 2.924 ± 0.709
1.81AsnGln: 1.81 ± 0.583
2.506AsnArg: 2.506 ± 0.619
4.177AsnSer: 4.177 ± 0.764
2.506AsnThr: 2.506 ± 0.618
1.949AsnVal: 1.949 ± 0.485
0.835AsnTrp: 0.835 ± 0.32
2.089AsnTyr: 2.089 ± 0.57
0.0AsnXaa: 0.0 ± 0.0
Pro
1.392ProAla: 1.392 ± 0.569
0.557ProCys: 0.557 ± 0.264
1.81ProAsp: 1.81 ± 0.475
2.228ProGlu: 2.228 ± 0.516
1.532ProPhe: 1.532 ± 0.594
0.696ProGly: 0.696 ± 0.255
0.418ProHis: 0.418 ± 0.231
2.924ProIle: 2.924 ± 0.771
2.646ProLys: 2.646 ± 0.676
1.949ProLeu: 1.949 ± 0.701
1.114ProMet: 1.114 ± 0.325
2.089ProAsn: 2.089 ± 0.555
1.671ProPro: 1.671 ± 0.561
1.253ProGln: 1.253 ± 0.498
1.114ProArg: 1.114 ± 0.427
3.063ProSer: 3.063 ± 0.853
1.81ProThr: 1.81 ± 0.584
1.949ProVal: 1.949 ± 0.433
0.139ProTrp: 0.139 ± 0.115
0.696ProTyr: 0.696 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
5.43GlnAla: 5.43 ± 0.761
0.278GlnCys: 0.278 ± 0.184
2.367GlnAsp: 2.367 ± 0.754
2.924GlnGlu: 2.924 ± 0.6
0.835GlnPhe: 0.835 ± 0.296
2.367GlnGly: 2.367 ± 0.632
0.975GlnHis: 0.975 ± 0.371
3.481GlnIle: 3.481 ± 0.734
3.759GlnLys: 3.759 ± 0.53
3.899GlnLeu: 3.899 ± 0.807
0.975GlnMet: 0.975 ± 0.336
2.089GlnAsn: 2.089 ± 0.584
1.392GlnPro: 1.392 ± 0.413
1.949GlnGln: 1.949 ± 0.651
1.81GlnArg: 1.81 ± 0.481
2.367GlnSer: 2.367 ± 0.587
2.785GlnThr: 2.785 ± 0.631
1.671GlnVal: 1.671 ± 0.513
0.278GlnTrp: 0.278 ± 0.209
1.114GlnTyr: 1.114 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
4.873ArgAla: 4.873 ± 0.683
0.557ArgCys: 0.557 ± 0.292
2.228ArgAsp: 2.228 ± 0.413
2.646ArgGlu: 2.646 ± 0.562
2.228ArgPhe: 2.228 ± 0.611
3.063ArgGly: 3.063 ± 0.763
0.975ArgHis: 0.975 ± 0.327
1.81ArgIle: 1.81 ± 0.472
2.089ArgLys: 2.089 ± 0.447
5.848ArgLeu: 5.848 ± 1.249
0.696ArgMet: 0.696 ± 0.237
1.949ArgAsn: 1.949 ± 0.461
1.532ArgPro: 1.532 ± 0.407
2.228ArgGln: 2.228 ± 0.508
1.81ArgArg: 1.81 ± 0.63
2.367ArgSer: 2.367 ± 0.645
2.506ArgThr: 2.506 ± 0.506
2.506ArgVal: 2.506 ± 0.483
0.835ArgTrp: 0.835 ± 0.326
3.481ArgTyr: 3.481 ± 0.714
0.0ArgXaa: 0.0 ± 0.0
Ser
6.126SerAla: 6.126 ± 0.906
0.418SerCys: 0.418 ± 0.257
3.063SerAsp: 3.063 ± 0.76
5.013SerGlu: 5.013 ± 0.74
2.228SerPhe: 2.228 ± 0.607
3.342SerGly: 3.342 ± 0.585
2.228SerHis: 2.228 ± 0.433
3.481SerIle: 3.481 ± 1.084
4.734SerLys: 4.734 ± 0.666
5.43SerLeu: 5.43 ± 0.89
1.81SerMet: 1.81 ± 0.545
3.759SerAsn: 3.759 ± 0.604
1.949SerPro: 1.949 ± 0.77
3.342SerGln: 3.342 ± 0.796
4.177SerArg: 4.177 ± 0.704
2.506SerSer: 2.506 ± 0.572
2.924SerThr: 2.924 ± 0.575
5.152SerVal: 5.152 ± 0.88
0.696SerTrp: 0.696 ± 0.302
1.532SerTyr: 1.532 ± 0.417
0.0SerXaa: 0.0 ± 0.0
Thr
6.823ThrAla: 6.823 ± 1.542
0.139ThrCys: 0.139 ± 0.153
4.038ThrAsp: 4.038 ± 0.848
3.62ThrGlu: 3.62 ± 0.769
1.392ThrPhe: 1.392 ± 0.429
5.013ThrGly: 5.013 ± 0.816
0.696ThrHis: 0.696 ± 0.363
3.063ThrIle: 3.063 ± 0.663
4.316ThrLys: 4.316 ± 0.892
5.013ThrLeu: 5.013 ± 0.843
1.671ThrMet: 1.671 ± 0.451
2.785ThrAsn: 2.785 ± 0.718
2.089ThrPro: 2.089 ± 0.694
2.646ThrGln: 2.646 ± 0.478
1.949ThrArg: 1.949 ± 0.591
2.924ThrSer: 2.924 ± 0.706
4.177ThrThr: 4.177 ± 0.94
4.456ThrVal: 4.456 ± 0.717
0.835ThrTrp: 0.835 ± 0.321
1.532ThrTyr: 1.532 ± 0.482
0.0ThrXaa: 0.0 ± 0.0
Val
5.013ValAla: 5.013 ± 1.083
1.392ValCys: 1.392 ± 0.518
2.785ValAsp: 2.785 ± 0.651
3.202ValGlu: 3.202 ± 0.514
1.392ValPhe: 1.392 ± 0.414
3.759ValGly: 3.759 ± 0.823
1.253ValHis: 1.253 ± 0.357
3.342ValIle: 3.342 ± 0.806
3.342ValLys: 3.342 ± 0.946
5.848ValLeu: 5.848 ± 0.627
1.532ValMet: 1.532 ± 0.379
2.506ValAsn: 2.506 ± 0.566
1.532ValPro: 1.532 ± 0.364
3.063ValGln: 3.063 ± 0.476
3.62ValArg: 3.62 ± 0.817
4.734ValSer: 4.734 ± 0.799
2.506ValThr: 2.506 ± 0.528
3.063ValVal: 3.063 ± 0.7
0.975ValTrp: 0.975 ± 0.407
2.089ValTyr: 2.089 ± 0.61
0.0ValXaa: 0.0 ± 0.0
Trp
1.253TrpAla: 1.253 ± 0.494
0.418TrpCys: 0.418 ± 0.292
0.696TrpAsp: 0.696 ± 0.247
0.696TrpGlu: 0.696 ± 0.243
0.0TrpPhe: 0.0 ± 0.0
0.835TrpGly: 0.835 ± 0.377
0.139TrpHis: 0.139 ± 0.099
0.418TrpIle: 0.418 ± 0.224
0.557TrpLys: 0.557 ± 0.343
1.114TrpLeu: 1.114 ± 0.477
0.278TrpMet: 0.278 ± 0.222
0.557TrpAsn: 0.557 ± 0.242
0.418TrpPro: 0.418 ± 0.322
1.949TrpGln: 1.949 ± 0.537
0.557TrpArg: 0.557 ± 0.318
0.835TrpSer: 0.835 ± 0.394
0.557TrpThr: 0.557 ± 0.278
0.696TrpVal: 0.696 ± 0.278
0.278TrpTrp: 0.278 ± 0.202
0.835TrpTyr: 0.835 ± 0.361
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.646TyrAla: 2.646 ± 0.866
0.696TyrCys: 0.696 ± 0.339
2.367TyrAsp: 2.367 ± 0.492
1.671TyrGlu: 1.671 ± 0.557
0.975TyrPhe: 0.975 ± 0.331
3.063TyrGly: 3.063 ± 0.542
0.835TyrHis: 0.835 ± 0.35
1.532TyrIle: 1.532 ± 0.48
1.949TyrLys: 1.949 ± 0.491
2.924TyrLeu: 2.924 ± 0.841
1.392TyrMet: 1.392 ± 0.545
0.696TyrAsn: 0.696 ± 0.316
1.253TyrPro: 1.253 ± 0.453
1.114TyrGln: 1.114 ± 0.358
2.506TyrArg: 2.506 ± 0.602
2.367TyrSer: 2.367 ± 0.608
1.532TyrThr: 1.532 ± 0.374
1.671TyrVal: 1.671 ± 0.496
0.139TyrTrp: 0.139 ± 0.147
1.949TyrTyr: 1.949 ± 0.554
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (7183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski