Cotton leaf curl Multan virus-[Faisalabad3]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Cotton leaf curl Multan virus

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IWB7|Q9IWB7_9GEMI Protein V2 OS=Cotton leaf curl Multan virus-[Faisalabad3] OX=223256 GN=v2 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.84FPDD11 pKa = 4.03TVHH14 pKa = 7.02GFRR17 pKa = 11.84CMLSVKK23 pKa = 9.95YY24 pKa = 10.2LQLLSQDD31 pKa = 3.9YY32 pKa = 11.37SPDD35 pKa = 3.3TLGYY39 pKa = 10.31EE40 pKa = 5.22LIRR43 pKa = 11.84DD44 pKa = 4.54LIGILRR50 pKa = 11.84SRR52 pKa = 11.84NYY54 pKa = 10.01VEE56 pKa = 4.34ASCRR60 pKa = 11.84YY61 pKa = 7.34SHH63 pKa = 7.34FYY65 pKa = 11.09ARR67 pKa = 11.84VEE69 pKa = 4.1STPACEE75 pKa = 4.04LRR77 pKa = 11.84QPIHH81 pKa = 6.2QPCCCPHH88 pKa = 6.61CPRR91 pKa = 11.84HH92 pKa = 5.62KK93 pKa = 8.3TTGMDD98 pKa = 3.07KK99 pKa = 10.37QAYY102 pKa = 6.7EE103 pKa = 4.01QEE105 pKa = 4.27AQNVV109 pKa = 3.47

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9IWB7|Q9IWB7_9GEMI Protein V2 OS=Cotton leaf curl Multan virus-[Faisalabad3] OX=223256 GN=v2 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.21RR4 pKa = 11.84AADD7 pKa = 3.55IVISTPASKK16 pKa = 10.31VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84VNFGSPYY27 pKa = 8.31TSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 9.91QQAWTNRR47 pKa = 11.84LMNRR51 pKa = 11.84KK52 pKa = 8.06PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.57RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.05VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.53VTRR97 pKa = 11.84GVGLTHH103 pKa = 7.8RR104 pKa = 11.84IGKK107 pKa = 8.64RR108 pKa = 11.84FCVKK112 pKa = 10.07SDD114 pKa = 3.53YY115 pKa = 11.28VLGKK119 pKa = 9.6IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.57NSHH172 pKa = 6.59RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 9.17QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 4.14AGKK218 pKa = 9.79HH219 pKa = 4.59EE220 pKa = 4.18NHH222 pKa = 6.64TEE224 pKa = 3.89NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.28DD252 pKa = 3.55SVPNN256 pKa = 3.97

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1104

94

361

184.0

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.344 ± 0.798

2.355 ± 0.571

4.801 ± 0.51

5.072 ± 0.719

4.348 ± 0.384

4.982 ± 0.263

3.895 ± 0.67

4.891 ± 0.535

5.435 ± 0.916

7.065 ± 1.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.661

5.888 ± 0.416

5.525 ± 0.472

5.978 ± 0.802

7.156 ± 0.79

7.699 ± 1.175

6.25 ± 0.983

5.978 ± 1.37

1.359 ± 0.12

3.895 ± 0.535
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski