Mycoplasma subdolum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 548 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L9ZXA8|A0A3L9ZXA8_9MOLU Pyrimidine-nucleoside phosphorylase OS=Mycoplasma subdolum OX=92407 GN=JN00_0463 PE=3 SV=1
MM1 pKa = 7.09YY2 pKa = 9.68RR3 pKa = 11.84DD4 pKa = 3.6KK5 pKa = 10.92KK6 pKa = 9.13TGRR9 pKa = 11.84LVWEE13 pKa = 3.99SAEE16 pKa = 3.9IEE18 pKa = 4.09EE19 pKa = 4.34AQTLKK24 pKa = 10.94SLNEE28 pKa = 3.93EE29 pKa = 4.17YY30 pKa = 10.28RR31 pKa = 11.84RR32 pKa = 11.84SPFYY36 pKa = 10.94KK37 pKa = 9.85KK38 pKa = 10.7GEE40 pKa = 4.22EE41 pKa = 4.28IISDD45 pKa = 3.58TSEE48 pKa = 3.64EE49 pKa = 4.07GLEE52 pKa = 4.2FFCNYY57 pKa = 10.62DD58 pKa = 3.19EE59 pKa = 4.74TKK61 pKa = 10.19IDD63 pKa = 5.19DD64 pKa = 4.75EE65 pKa = 5.83DD66 pKa = 6.51DD67 pKa = 3.85EE68 pKa = 7.27DD69 pKa = 6.28DD70 pKa = 6.33DD71 pKa = 4.8YY72 pKa = 12.23EE73 pKa = 4.36EE74 pKa = 4.66YY75 pKa = 11.23NN76 pKa = 3.6

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M0A1W5|A0A3M0A1W5_9MOLU Holliday junction ATP-dependent DNA helicase RuvB OS=Mycoplasma subdolum OX=92407 GN=ruvB PE=3 SV=1
MM1 pKa = 7.58AKK3 pKa = 10.05KK4 pKa = 10.46SLMVKK9 pKa = 10.38AEE11 pKa = 4.19RR12 pKa = 11.84KK13 pKa = 9.35PKK15 pKa = 10.28FKK17 pKa = 10.44ARR19 pKa = 11.84KK20 pKa = 6.08YY21 pKa = 8.42TRR23 pKa = 11.84CQICGRR29 pKa = 11.84VHH31 pKa = 6.76AVLRR35 pKa = 11.84KK36 pKa = 9.58YY37 pKa = 10.23KK38 pKa = 9.85ICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.88LAHH49 pKa = 6.54EE50 pKa = 4.41GKK52 pKa = 10.21IPGVKK57 pKa = 9.38KK58 pKa = 10.89ASWW61 pKa = 3.03

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

548

0

548

195964

37

2923

357.6

40.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.862 ± 0.102

0.598 ± 0.027

5.459 ± 0.086

7.49 ± 0.094

5.36 ± 0.094

4.756 ± 0.102

1.433 ± 0.038

9.24 ± 0.094

11.215 ± 0.129

9.553 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.047

6.598 ± 0.101

2.752 ± 0.057

3.116 ± 0.053

3.014 ± 0.063

6.107 ± 0.079

5.226 ± 0.074

5.697 ± 0.073

0.9 ± 0.033

3.808 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski