Amino acid dipepetide frequency for Mycoplasma subdolum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.276AlaAla: 3.276 ± 0.181
0.357AlaCys: 0.357 ± 0.043
2.465AlaAsp: 2.465 ± 0.122
3.424AlaGlu: 3.424 ± 0.142
2.919AlaPhe: 2.919 ± 0.142
2.786AlaGly: 2.786 ± 0.148
0.76AlaHis: 0.76 ± 0.065
6.006AlaIle: 6.006 ± 0.226
7.522AlaLys: 7.522 ± 0.28
6.098AlaLeu: 6.098 ± 0.244
1.194AlaMet: 1.194 ± 0.09
3.751AlaAsn: 3.751 ± 0.175
1.199AlaPro: 1.199 ± 0.087
1.857AlaGln: 1.857 ± 0.128
2.21AlaArg: 2.21 ± 0.117
3.705AlaSer: 3.705 ± 0.124
3.608AlaThr: 3.608 ± 0.163
2.847AlaVal: 2.847 ± 0.158
0.5AlaTrp: 0.5 ± 0.049
2.133AlaTyr: 2.133 ± 0.128
0.0AlaXaa: 0.0 ± 0.0
Cys
0.398CysAla: 0.398 ± 0.048
0.077CysCys: 0.077 ± 0.019
0.311CysAsp: 0.311 ± 0.048
0.536CysGlu: 0.536 ± 0.048
0.403CysPhe: 0.403 ± 0.042
0.373CysGly: 0.373 ± 0.048
0.122CysHis: 0.122 ± 0.026
0.393CysIle: 0.393 ± 0.054
0.572CysLys: 0.572 ± 0.056
0.663CysLeu: 0.663 ± 0.07
0.112CysMet: 0.112 ± 0.025
0.311CysAsn: 0.311 ± 0.039
0.209CysPro: 0.209 ± 0.037
0.204CysGln: 0.204 ± 0.033
0.174CysArg: 0.174 ± 0.033
0.296CysSer: 0.296 ± 0.04
0.209CysThr: 0.209 ± 0.035
0.342CysVal: 0.342 ± 0.045
0.041CysTrp: 0.041 ± 0.015
0.23CysTyr: 0.23 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
3.735AspAla: 3.735 ± 0.196
0.174AspCys: 0.174 ± 0.03
2.776AspAsp: 2.776 ± 0.145
5.134AspGlu: 5.134 ± 0.175
3.98AspPhe: 3.98 ± 0.159
2.781AspGly: 2.781 ± 0.127
0.653AspHis: 0.653 ± 0.068
4.353AspIle: 4.353 ± 0.155
6.119AspLys: 6.119 ± 0.209
5.654AspLeu: 5.654 ± 0.196
0.806AspMet: 0.806 ± 0.063
2.96AspAsn: 2.96 ± 0.14
1.567AspPro: 1.567 ± 0.077
1.388AspGln: 1.388 ± 0.075
1.281AspArg: 1.281 ± 0.077
2.995AspSer: 2.995 ± 0.126
2.077AspThr: 2.077 ± 0.115
3.179AspVal: 3.179 ± 0.121
0.541AspTrp: 0.541 ± 0.055
2.439AspTyr: 2.439 ± 0.128
0.0AspXaa: 0.0 ± 0.0
Glu
4.455GluAla: 4.455 ± 0.222
0.327GluCys: 0.327 ± 0.049
3.644GluAsp: 3.644 ± 0.137
6.583GluGlu: 6.583 ± 0.21
3.832GluPhe: 3.832 ± 0.153
3.098GluGly: 3.098 ± 0.139
1.005GluHis: 1.005 ± 0.076
8.047GluIle: 8.047 ± 0.214
9.349GluLys: 9.349 ± 0.243
6.726GluLeu: 6.726 ± 0.201
1.454GluMet: 1.454 ± 0.103
5.756GluAsn: 5.756 ± 0.211
1.419GluPro: 1.419 ± 0.097
2.062GluGln: 2.062 ± 0.114
2.281GluArg: 2.281 ± 0.138
3.215GluSer: 3.215 ± 0.123
3.822GluThr: 3.822 ± 0.137
4.475GluVal: 4.475 ± 0.169
0.597GluTrp: 0.597 ± 0.059
2.751GluTyr: 2.751 ± 0.11
0.0GluXaa: 0.0 ± 0.0
Phe
3.291PheAla: 3.291 ± 0.153
0.357PheCys: 0.357 ± 0.044
3.679PheAsp: 3.679 ± 0.143
4.006PheGlu: 4.006 ± 0.187
2.679PhePhe: 2.679 ± 0.155
2.883PheGly: 2.883 ± 0.142
0.704PheHis: 0.704 ± 0.062
4.787PheIle: 4.787 ± 0.207
5.654PheLys: 5.654 ± 0.214
4.822PheLeu: 4.822 ± 0.223
0.878PheMet: 0.878 ± 0.065
3.496PheAsn: 3.496 ± 0.164
1.245PhePro: 1.245 ± 0.089
1.352PheGln: 1.352 ± 0.091
1.48PheArg: 1.48 ± 0.083
3.883PheSer: 3.883 ± 0.169
2.531PheThr: 2.531 ± 0.112
3.215PheVal: 3.215 ± 0.146
0.577PheTrp: 0.577 ± 0.062
2.082PheTyr: 2.082 ± 0.112
0.0PheXaa: 0.0 ± 0.0
Gly
2.679GlyAla: 2.679 ± 0.136
0.189GlyCys: 0.189 ± 0.032
2.337GlyAsp: 2.337 ± 0.115
3.113GlyGlu: 3.113 ± 0.141
2.807GlyPhe: 2.807 ± 0.133
2.832GlyGly: 2.832 ± 0.168
1.0GlyHis: 1.0 ± 0.077
4.47GlyIle: 4.47 ± 0.189
5.669GlyLys: 5.669 ± 0.19
4.123GlyLeu: 4.123 ± 0.155
0.995GlyMet: 0.995 ± 0.085
2.143GlyAsn: 2.143 ± 0.116
1.051GlyPro: 1.051 ± 0.082
1.556GlyGln: 1.556 ± 0.094
1.832GlyArg: 1.832 ± 0.111
2.919GlySer: 2.919 ± 0.125
2.74GlyThr: 2.74 ± 0.142
2.771GlyVal: 2.771 ± 0.135
0.551GlyTrp: 0.551 ± 0.059
1.786GlyTyr: 1.786 ± 0.109
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.067
0.092HisCys: 0.092 ± 0.022
0.776HisAsp: 0.776 ± 0.061
1.005HisGlu: 1.005 ± 0.069
0.857HisPhe: 0.857 ± 0.072
0.929HisGly: 0.929 ± 0.071
0.24HisHis: 0.24 ± 0.036
1.087HisIle: 1.087 ± 0.08
1.357HisLys: 1.357 ± 0.069
1.245HisLeu: 1.245 ± 0.083
0.235HisMet: 0.235 ± 0.037
0.975HisAsn: 0.975 ± 0.072
0.674HisPro: 0.674 ± 0.068
0.459HisGln: 0.459 ± 0.048
0.434HisArg: 0.434 ± 0.05
0.822HisSer: 0.822 ± 0.072
0.714HisThr: 0.714 ± 0.059
0.801HisVal: 0.801 ± 0.071
0.179HisTrp: 0.179 ± 0.028
0.592HisTyr: 0.592 ± 0.063
0.0HisXaa: 0.0 ± 0.0
Ile
5.312IleAla: 5.312 ± 0.173
0.704IleCys: 0.704 ± 0.063
5.608IleAsp: 5.608 ± 0.209
6.751IleGlu: 6.751 ± 0.206
4.93IlePhe: 4.93 ± 0.206
4.225IleGly: 4.225 ± 0.18
1.204IleHis: 1.204 ± 0.068
8.139IleIle: 8.139 ± 0.253
9.941IleLys: 9.941 ± 0.261
8.343IleLeu: 8.343 ± 0.233
1.419IleMet: 1.419 ± 0.086
6.67IleAsn: 6.67 ± 0.239
2.608IlePro: 2.608 ± 0.129
2.689IleGln: 2.689 ± 0.128
2.802IleArg: 2.802 ± 0.123
6.695IleSer: 6.695 ± 0.21
4.297IleThr: 4.297 ± 0.145
5.72IleVal: 5.72 ± 0.2
0.745IleTrp: 0.745 ± 0.07
3.598IleTyr: 3.598 ± 0.148
0.0IleXaa: 0.0 ± 0.0
Lys
6.573LysAla: 6.573 ± 0.314
0.403LysCys: 0.403 ± 0.054
6.649LysAsp: 6.649 ± 0.23
9.303LysGlu: 9.303 ± 0.273
4.889LysPhe: 4.889 ± 0.185
4.082LysGly: 4.082 ± 0.166
1.48LysHis: 1.48 ± 0.084
11.068LysIle: 11.068 ± 0.258
12.074LysLys: 12.074 ± 0.336
10.374LysLeu: 10.374 ± 0.262
2.531LysMet: 2.531 ± 0.108
8.098LysAsn: 8.098 ± 0.231
3.194LysPro: 3.194 ± 0.141
3.97LysGln: 3.97 ± 0.167
3.215LysArg: 3.215 ± 0.145
5.782LysSer: 5.782 ± 0.173
6.629LysThr: 6.629 ± 0.207
6.517LysVal: 6.517 ± 0.218
0.954LysTrp: 0.954 ± 0.073
4.363LysTyr: 4.363 ± 0.147
0.0LysXaa: 0.0 ± 0.0
Leu
5.95LeuAla: 5.95 ± 0.202
0.638LeuCys: 0.638 ± 0.058
5.69LeuAsp: 5.69 ± 0.18
7.17LeuGlu: 7.17 ± 0.214
4.69LeuPhe: 4.69 ± 0.202
4.664LeuGly: 4.664 ± 0.176
1.215LeuHis: 1.215 ± 0.079
9.18LeuIle: 9.18 ± 0.256
10.262LeuLys: 10.262 ± 0.254
8.185LeuLeu: 8.185 ± 0.245
1.75LeuMet: 1.75 ± 0.101
7.057LeuAsn: 7.057 ± 0.309
2.99LeuPro: 2.99 ± 0.139
2.71LeuGln: 2.71 ± 0.12
2.802LeuArg: 2.802 ± 0.131
6.323LeuSer: 6.323 ± 0.211
5.613LeuThr: 5.613 ± 0.172
5.297LeuVal: 5.297 ± 0.164
0.612LeuTrp: 0.612 ± 0.055
2.73LeuTyr: 2.73 ± 0.121
0.0LeuXaa: 0.0 ± 0.0
Met
1.311MetAla: 1.311 ± 0.09
0.133MetCys: 0.133 ± 0.027
0.811MetAsp: 0.811 ± 0.066
1.082MetGlu: 1.082 ± 0.073
1.005MetPhe: 1.005 ± 0.08
0.668MetGly: 0.668 ± 0.06
0.306MetHis: 0.306 ± 0.04
1.669MetIle: 1.669 ± 0.105
2.164MetLys: 2.164 ± 0.099
1.766MetLeu: 1.766 ± 0.103
0.388MetMet: 0.388 ± 0.046
1.143MetAsn: 1.143 ± 0.083
0.745MetPro: 0.745 ± 0.068
0.704MetGln: 0.704 ± 0.059
0.612MetArg: 0.612 ± 0.05
1.189MetSer: 1.189 ± 0.071
0.913MetThr: 0.913 ± 0.061
0.888MetVal: 0.888 ± 0.064
0.122MetTrp: 0.122 ± 0.028
0.541MetTyr: 0.541 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
3.434AsnAla: 3.434 ± 0.174
0.413AsnCys: 0.413 ± 0.054
3.531AsnAsp: 3.531 ± 0.155
5.251AsnGlu: 5.251 ± 0.185
3.776AsnPhe: 3.776 ± 0.17
2.96AsnGly: 2.96 ± 0.12
0.878AsnHis: 0.878 ± 0.066
5.624AsnIle: 5.624 ± 0.194
7.563AsnLys: 7.563 ± 0.222
7.558AsnLeu: 7.558 ± 0.263
0.995AsnMet: 0.995 ± 0.081
4.021AsnAsn: 4.021 ± 0.176
2.363AsnPro: 2.363 ± 0.104
2.424AsnGln: 2.424 ± 0.128
1.521AsnArg: 1.521 ± 0.092
3.98AsnSer: 3.98 ± 0.131
2.383AsnThr: 2.383 ± 0.112
3.654AsnVal: 3.654 ± 0.123
0.75AsnTrp: 0.75 ± 0.068
2.904AsnTyr: 2.904 ± 0.16
0.0AsnXaa: 0.0 ± 0.0
Pro
1.572ProAla: 1.572 ± 0.106
0.158ProCys: 0.158 ± 0.03
1.388ProAsp: 1.388 ± 0.083
2.077ProGlu: 2.077 ± 0.108
1.526ProPhe: 1.526 ± 0.092
1.301ProGly: 1.301 ± 0.093
0.526ProHis: 0.526 ± 0.049
2.429ProIle: 2.429 ± 0.113
2.842ProLys: 2.842 ± 0.117
2.465ProLeu: 2.465 ± 0.085
0.403ProMet: 0.403 ± 0.05
1.888ProAsn: 1.888 ± 0.098
0.51ProPro: 0.51 ± 0.053
0.898ProGln: 0.898 ± 0.077
0.704ProArg: 0.704 ± 0.069
1.791ProSer: 1.791 ± 0.112
1.868ProThr: 1.868 ± 0.112
1.628ProVal: 1.628 ± 0.102
0.311ProTrp: 0.311 ± 0.044
1.23ProTyr: 1.23 ± 0.081
0.0ProXaa: 0.0 ± 0.0
Gln
2.23GlnAla: 2.23 ± 0.121
0.097GlnCys: 0.097 ± 0.024
1.786GlnAsp: 1.786 ± 0.114
2.511GlnGlu: 2.511 ± 0.122
1.215GlnPhe: 1.215 ± 0.088
1.169GlnGly: 1.169 ± 0.073
0.464GlnHis: 0.464 ± 0.046
3.128GlnIle: 3.128 ± 0.125
3.945GlnLys: 3.945 ± 0.167
2.332GlnLeu: 2.332 ± 0.115
0.566GlnMet: 0.566 ± 0.057
2.567GlnAsn: 2.567 ± 0.112
0.755GlnPro: 0.755 ± 0.067
0.903GlnGln: 0.903 ± 0.076
1.005GlnArg: 1.005 ± 0.077
1.521GlnSer: 1.521 ± 0.091
1.75GlnThr: 1.75 ± 0.091
1.929GlnVal: 1.929 ± 0.108
0.209GlnTrp: 0.209 ± 0.034
1.082GlnTyr: 1.082 ± 0.081
0.0GlnXaa: 0.0 ± 0.0
Arg
1.653ArgAla: 1.653 ± 0.095
0.199ArgCys: 0.199 ± 0.033
1.643ArgAsp: 1.643 ± 0.102
2.276ArgGlu: 2.276 ± 0.139
1.587ArgPhe: 1.587 ± 0.099
1.562ArgGly: 1.562 ± 0.105
0.48ArgHis: 0.48 ± 0.047
2.766ArgIle: 2.766 ± 0.128
3.297ArgLys: 3.297 ± 0.141
2.659ArgLeu: 2.659 ± 0.11
0.699ArgMet: 0.699 ± 0.06
1.98ArgAsn: 1.98 ± 0.109
0.837ArgPro: 0.837 ± 0.071
1.184ArgGln: 1.184 ± 0.069
1.128ArgArg: 1.128 ± 0.089
1.567ArgSer: 1.567 ± 0.092
1.398ArgThr: 1.398 ± 0.078
1.837ArgVal: 1.837 ± 0.102
0.27ArgTrp: 0.27 ± 0.039
1.118ArgTyr: 1.118 ± 0.085
0.0ArgXaa: 0.0 ± 0.0
Ser
3.113SerAla: 3.113 ± 0.145
0.556SerCys: 0.556 ± 0.051
3.046SerAsp: 3.046 ± 0.136
4.016SerGlu: 4.016 ± 0.139
3.781SerPhe: 3.781 ± 0.172
3.184SerGly: 3.184 ± 0.143
0.929SerHis: 0.929 ± 0.075
5.256SerIle: 5.256 ± 0.191
7.231SerLys: 7.231 ± 0.209
6.527SerLeu: 6.527 ± 0.201
1.061SerMet: 1.061 ± 0.075
3.562SerAsn: 3.562 ± 0.121
1.556SerPro: 1.556 ± 0.095
2.118SerGln: 2.118 ± 0.115
1.832SerArg: 1.832 ± 0.119
4.128SerSer: 4.128 ± 0.171
3.164SerThr: 3.164 ± 0.125
3.092SerVal: 3.092 ± 0.137
0.5SerTrp: 0.5 ± 0.061
2.419SerTyr: 2.419 ± 0.133
0.0SerXaa: 0.0 ± 0.0
Thr
2.654ThrAla: 2.654 ± 0.135
0.408ThrCys: 0.408 ± 0.049
2.215ThrAsp: 2.215 ± 0.12
2.669ThrGlu: 2.669 ± 0.121
3.2ThrPhe: 3.2 ± 0.156
2.699ThrGly: 2.699 ± 0.139
0.893ThrHis: 0.893 ± 0.066
4.955ThrIle: 4.955 ± 0.188
5.858ThrLys: 5.858 ± 0.21
5.695ThrLeu: 5.695 ± 0.183
0.694ThrMet: 0.694 ± 0.063
3.235ThrAsn: 3.235 ± 0.125
1.771ThrPro: 1.771 ± 0.108
1.669ThrGln: 1.669 ± 0.1
1.633ThrArg: 1.633 ± 0.092
3.654ThrSer: 3.654 ± 0.139
3.046ThrThr: 3.046 ± 0.146
2.679ThrVal: 2.679 ± 0.121
0.521ThrTrp: 0.521 ± 0.061
2.113ThrTyr: 2.113 ± 0.105
0.0ThrXaa: 0.0 ± 0.0
Val
3.654ValAla: 3.654 ± 0.158
0.439ValCys: 0.439 ± 0.051
3.536ValAsp: 3.536 ± 0.148
4.358ValGlu: 4.358 ± 0.168
2.71ValPhe: 2.71 ± 0.124
3.021ValGly: 3.021 ± 0.147
0.791ValHis: 0.791 ± 0.068
4.853ValIle: 4.853 ± 0.194
6.052ValLys: 6.052 ± 0.224
5.205ValLeu: 5.205 ± 0.193
1.036ValMet: 1.036 ± 0.07
3.557ValAsn: 3.557 ± 0.129
1.607ValPro: 1.607 ± 0.094
1.679ValGln: 1.679 ± 0.091
1.592ValArg: 1.592 ± 0.087
3.7ValSer: 3.7 ± 0.145
3.225ValThr: 3.225 ± 0.153
3.541ValVal: 3.541 ± 0.143
0.429ValTrp: 0.429 ± 0.049
1.985ValTyr: 1.985 ± 0.106
0.0ValXaa: 0.0 ± 0.0
Trp
0.418TrpAla: 0.418 ± 0.044
0.031TrpCys: 0.031 ± 0.015
0.469TrpAsp: 0.469 ± 0.048
0.689TrpGlu: 0.689 ± 0.057
0.469TrpPhe: 0.469 ± 0.044
0.398TrpGly: 0.398 ± 0.046
0.112TrpHis: 0.112 ± 0.027
0.883TrpIle: 0.883 ± 0.068
1.082TrpLys: 1.082 ± 0.07
0.832TrpLeu: 0.832 ± 0.067
0.265TrpMet: 0.265 ± 0.035
0.602TrpAsn: 0.602 ± 0.056
0.27TrpPro: 0.27 ± 0.035
0.281TrpGln: 0.281 ± 0.04
0.306TrpArg: 0.306 ± 0.042
0.475TrpSer: 0.475 ± 0.051
0.5TrpThr: 0.5 ± 0.047
0.413TrpVal: 0.413 ± 0.047
0.158TrpTrp: 0.158 ± 0.029
0.342TrpTyr: 0.342 ± 0.045
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.046TyrAla: 2.046 ± 0.108
0.225TyrCys: 0.225 ± 0.037
2.23TyrAsp: 2.23 ± 0.115
2.944TyrGlu: 2.944 ± 0.112
2.347TyrPhe: 2.347 ± 0.142
1.949TyrGly: 1.949 ± 0.108
0.572TyrHis: 0.572 ± 0.059
3.307TyrIle: 3.307 ± 0.132
3.603TyrLys: 3.603 ± 0.142
4.256TyrLeu: 4.256 ± 0.147
0.674TyrMet: 0.674 ± 0.065
2.21TyrAsn: 2.21 ± 0.113
0.944TyrPro: 0.944 ± 0.074
1.056TyrGln: 1.056 ± 0.066
1.296TyrArg: 1.296 ± 0.09
2.434TyrSer: 2.434 ± 0.127
1.771TyrThr: 1.771 ± 0.111
2.143TyrVal: 2.143 ± 0.108
0.429TyrTrp: 0.429 ± 0.047
1.643TyrTyr: 1.643 ± 0.107
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 548 proteins (195964 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski