Philantomba monticola polyomavirus 1
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1CJI1|A0A2S1CJI1_9POLY Uncharacterized protein OS=Philantomba monticola polyomavirus 1 OX=2170411 PE=4 SV=1
MM1 pKa = 7.26 GAIISSLVEE10 pKa = 4.05 MIALATEE17 pKa = 4.41 ISAASGLTIEE27 pKa = 5.1 ALLTGEE33 pKa = 4.43 ALGALEE39 pKa = 5.17 AEE41 pKa = 4.86 VFSLMSIEE49 pKa = 4.04 GLSGIEE55 pKa = 4.56 ALAQLGWTAEE65 pKa = 4.13 QFSNLAFIASTFSDD79 pKa = 3.81 VVGYY83 pKa = 10.32 GVFFQTTSGLASLISVGLRR102 pKa = 11.84 LGLDD106 pKa = 3.35 VASVNRR112 pKa = 11.84 NQTEE116 pKa = 4.0 QTLLAIFGEE125 pKa = 4.56 VSKK128 pKa = 10.56 LIPINISYY136 pKa = 10.25 HH137 pKa = 5.93 LNPLKK142 pKa = 10.42 WEE144 pKa = 4.21 EE145 pKa = 4.11 SLKK148 pKa = 10.15 TNCPKK153 pKa = 10.67 DD154 pKa = 3.74 LEE156 pKa = 4.7 NIPIEE161 pKa = 3.95 MRR163 pKa = 11.84 HH164 pKa = 5.68 IIGVLMQNSRR174 pKa = 11.84 WVIQSSPTTDD184 pKa = 3.38 PQKK187 pKa = 10.61 EE188 pKa = 4.15 SGDD191 pKa = 3.22 IVEE194 pKa = 4.9 FQPPPGGANQPATPDD209 pKa = 2.83 WMLPLILRR217 pKa = 11.84 LNGASKK223 pKa = 10.47 EE224 pKa = 4.05 KK225 pKa = 10.87 SSLCSS230 pKa = 3.25
Molecular weight: 24.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.611
IPC2_protein 4.546
IPC_protein 4.431
Toseland 4.291
ProMoST 4.507
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.19
Rodwell 4.279
Grimsley 4.202
Solomon 4.355
Lehninger 4.304
Nozaki 4.469
DTASelect 4.546
Thurlkill 4.279
EMBOSS 4.215
Sillero 4.533
Patrickios 3.948
IPC_peptide 4.368
IPC2_peptide 4.533
IPC2.peptide.svr19 4.466
Protein with the highest isoelectric point:
>tr|A0A2S1CJK0|A0A2S1CJK0_9POLY VP1 OS=Philantomba monticola polyomavirus 1 OX=2170411 GN=VP1 PE=3 SV=1
MM1 pKa = 7.59 ARR3 pKa = 11.84 RR4 pKa = 11.84 PSEE7 pKa = 3.82 RR8 pKa = 11.84 DD9 pKa = 3.0 IEE11 pKa = 4.49 NGAPQYY17 pKa = 11.56 LPMKK21 pKa = 9.31 NLNSEE26 pKa = 4.37 EE27 pKa = 4.74 IYY29 pKa = 10.71 TVMSPLSPVKK39 pKa = 10.56 KK40 pKa = 10.08 KK41 pKa = 7.17 MHH43 pKa = 5.93 RR44 pKa = 11.84 NSRR47 pKa = 11.84 KK48 pKa = 9.92 SLDD51 pKa = 3.55 ITQHH55 pKa = 6.25 PSLLPFPTPPMNPGPPPPLPPKK77 pKa = 10.13 RR78 pKa = 11.84 IRR80 pKa = 11.84 RR81 pKa = 11.84 SVSLNPTPPPRR92 pKa = 11.84 VQLLEE97 pKa = 4.46 DD98 pKa = 3.55 EE99 pKa = 4.57 VRR101 pKa = 11.84 DD102 pKa = 4.69 LKK104 pKa = 11.29 EE105 pKa = 3.81 KK106 pKa = 9.95 LQKK109 pKa = 10.01 RR110 pKa = 11.84 DD111 pKa = 3.22 EE112 pKa = 4.8 DD113 pKa = 4.75 LLKK116 pKa = 10.04 TWMNLIQARR125 pKa = 11.84 KK126 pKa = 8.89 LALQAHH132 pKa = 5.59 RR133 pKa = 11.84 QKK135 pKa = 10.7 QKK137 pKa = 10.53 RR138 pKa = 11.84 QALTVLMIFLLVCLILSVMLYY159 pKa = 9.85 FLIRR163 pKa = 11.84 QQ164 pKa = 3.56
Molecular weight: 19.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.604
IPC_protein 9.97
Toseland 10.716
ProMoST 10.277
Dawson 10.789
Bjellqvist 10.423
Wikipedia 10.95
Rodwell 11.184
Grimsley 10.818
Solomon 10.877
Lehninger 10.862
Nozaki 10.687
DTASelect 10.423
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.921
IPC_peptide 10.891
IPC2_peptide 9.033
IPC2.peptide.svr19 8.753
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2841
92
776
355.1
39.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.054 ± 0.46
3.203 ± 0.478
4.646 ± 0.46
6.969 ± 0.467
5.174 ± 0.645
5.561 ± 0.787
2.006 ± 0.186
4.928 ± 0.469
7.251 ± 0.527
10.736 ± 0.632
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.006 ± 0.365
4.541 ± 0.108
5.843 ± 0.883
3.907 ± 0.366
4.189 ± 0.368
7.92 ± 0.73
5.421 ± 0.236
5.491 ± 0.297
1.338 ± 0.233
2.816 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here