Streptococcus phage Javan348

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B479|A0A4D6B479_9CAUD Cell wall-associated murein hydrolase OS=Streptococcus phage Javan348 OX=2548118 GN=Javan348_0049 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.42LSDD5 pKa = 3.55FAVILEE11 pKa = 4.11QANLPVTYY19 pKa = 10.15RR20 pKa = 11.84AFKK23 pKa = 10.28IGNAPDD29 pKa = 3.85LPYY32 pKa = 10.75LVYY35 pKa = 10.87YY36 pKa = 10.0EE37 pKa = 4.6SSPAINAADD46 pKa = 3.39NTVNHH51 pKa = 6.28QIKK54 pKa = 10.48SVTVEE59 pKa = 3.76LAFEE63 pKa = 4.26QKK65 pKa = 10.82DD66 pKa = 3.4EE67 pKa = 4.3DD68 pKa = 3.79LEE70 pKa = 4.25EE71 pKa = 4.71RR72 pKa = 11.84LEE74 pKa = 4.24EE75 pKa = 3.82LWTTHH80 pKa = 6.24EE81 pKa = 4.66LFFDD85 pKa = 3.8VQEE88 pKa = 4.16EE89 pKa = 4.62TFIEE93 pKa = 4.21AEE95 pKa = 3.77RR96 pKa = 11.84LYY98 pKa = 10.53VKK100 pKa = 10.44SYY102 pKa = 9.52TVYY105 pKa = 10.87LYY107 pKa = 11.2

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B105|A0A4D6B105_9CAUD Uncharacterized protein OS=Streptococcus phage Javan348 OX=2548118 GN=Javan348_0051 PE=4 SV=1
MM1 pKa = 7.74FILKK5 pKa = 10.18HH6 pKa = 4.74GTKK9 pKa = 10.06EE10 pKa = 3.89EE11 pKa = 4.04KK12 pKa = 9.97PYY14 pKa = 11.29LMSAKK19 pKa = 10.03IGVTGIDD26 pKa = 2.8ISFSEE31 pKa = 4.21EE32 pKa = 3.18RR33 pKa = 11.84GAIRR37 pKa = 11.84FVSRR41 pKa = 11.84AVALQVAKK49 pKa = 10.72ALRR52 pKa = 11.84SFGNFYY58 pKa = 10.66VIQVKK63 pKa = 10.07GG64 pKa = 3.29

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

9381

40

1036

180.4

20.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.566 ± 0.538

0.544 ± 0.11

5.97 ± 0.296

8.325 ± 0.427

4.115 ± 0.303

5.831 ± 0.283

1.61 ± 0.175

7.579 ± 0.318

8.634 ± 0.411

8.336 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.21

5.714 ± 0.295

2.814 ± 0.235

4.317 ± 0.301

4.243 ± 0.272

5.82 ± 0.248

5.906 ± 0.293

5.991 ± 0.303

1.332 ± 0.156

3.656 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski