Amino acid dipepetide frequency for Streptococcus phage Javan348

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.945AlaAla: 3.945 ± 1.127
0.64AlaCys: 0.64 ± 0.227
5.117AlaAsp: 5.117 ± 0.53
5.65AlaGlu: 5.65 ± 0.669
2.132AlaPhe: 2.132 ± 0.407
3.731AlaGly: 3.731 ± 0.495
0.959AlaHis: 0.959 ± 0.328
6.93AlaIle: 6.93 ± 1.2
4.797AlaLys: 4.797 ± 0.709
6.397AlaLeu: 6.397 ± 0.932
1.812AlaMet: 1.812 ± 0.402
3.198AlaAsn: 3.198 ± 0.747
1.066AlaPro: 1.066 ± 0.258
3.305AlaGln: 3.305 ± 0.669
2.132AlaArg: 2.132 ± 0.482
3.198AlaSer: 3.198 ± 0.516
2.985AlaThr: 2.985 ± 0.5
4.051AlaVal: 4.051 ± 0.748
0.959AlaTrp: 0.959 ± 0.351
2.665AlaTyr: 2.665 ± 0.59
0.0AlaXaa: 0.0 ± 0.0
Cys
0.107CysAla: 0.107 ± 0.121
0.0CysCys: 0.0 ± 0.0
0.107CysAsp: 0.107 ± 0.094
0.64CysGlu: 0.64 ± 0.285
0.107CysPhe: 0.107 ± 0.114
0.32CysGly: 0.32 ± 0.201
0.32CysHis: 0.32 ± 0.213
0.32CysIle: 0.32 ± 0.219
0.213CysLys: 0.213 ± 0.145
0.746CysLeu: 0.746 ± 0.321
0.213CysMet: 0.213 ± 0.176
0.533CysAsn: 0.533 ± 0.266
0.0CysPro: 0.0 ± 0.0
0.426CysGln: 0.426 ± 0.204
0.107CysArg: 0.107 ± 0.123
0.213CysSer: 0.213 ± 0.156
0.32CysThr: 0.32 ± 0.231
0.107CysVal: 0.107 ± 0.091
0.213CysTrp: 0.213 ± 0.155
0.426CysTyr: 0.426 ± 0.172
0.0CysXaa: 0.0 ± 0.0
Asp
3.092AspAla: 3.092 ± 0.453
0.32AspCys: 0.32 ± 0.249
2.878AspAsp: 2.878 ± 0.696
4.584AspGlu: 4.584 ± 0.675
2.132AspPhe: 2.132 ± 0.365
4.904AspGly: 4.904 ± 0.957
0.853AspHis: 0.853 ± 0.277
6.61AspIle: 6.61 ± 0.74
4.478AspLys: 4.478 ± 0.548
5.544AspLeu: 5.544 ± 0.717
1.812AspMet: 1.812 ± 0.345
3.198AspAsn: 3.198 ± 0.488
2.132AspPro: 2.132 ± 0.443
1.493AspGln: 1.493 ± 0.336
2.345AspArg: 2.345 ± 0.553
3.305AspSer: 3.305 ± 0.663
2.985AspThr: 2.985 ± 0.572
3.412AspVal: 3.412 ± 0.573
0.64AspTrp: 0.64 ± 0.213
2.985AspTyr: 2.985 ± 0.613
0.0AspXaa: 0.0 ± 0.0
Glu
5.864GluAla: 5.864 ± 0.935
0.0GluCys: 0.0 ± 0.0
3.198GluAsp: 3.198 ± 0.762
7.036GluGlu: 7.036 ± 1.326
3.305GluPhe: 3.305 ± 0.758
3.198GluGly: 3.198 ± 0.537
1.173GluHis: 1.173 ± 0.36
7.036GluIle: 7.036 ± 0.817
7.889GluLys: 7.889 ± 1.072
10.235GluLeu: 10.235 ± 1.119
3.092GluMet: 3.092 ± 0.71
3.945GluAsn: 3.945 ± 0.623
1.386GluPro: 1.386 ± 0.597
4.371GluGln: 4.371 ± 0.736
3.198GluArg: 3.198 ± 0.459
4.051GluSer: 4.051 ± 0.614
5.224GluThr: 5.224 ± 0.683
5.117GluVal: 5.117 ± 0.686
1.279GluTrp: 1.279 ± 0.343
2.665GluTyr: 2.665 ± 0.469
0.0GluXaa: 0.0 ± 0.0
Phe
2.026PheAla: 2.026 ± 0.475
0.213PheCys: 0.213 ± 0.172
2.559PheAsp: 2.559 ± 0.468
2.665PheGlu: 2.665 ± 0.555
1.812PhePhe: 1.812 ± 0.55
3.092PheGly: 3.092 ± 0.553
0.426PheHis: 0.426 ± 0.221
4.158PheIle: 4.158 ± 0.567
4.584PheLys: 4.584 ± 0.563
2.985PheLeu: 2.985 ± 0.571
1.493PheMet: 1.493 ± 0.358
2.985PheAsn: 2.985 ± 0.426
0.959PhePro: 0.959 ± 0.347
1.279PheGln: 1.279 ± 0.344
1.493PheArg: 1.493 ± 0.303
2.026PheSer: 2.026 ± 0.37
1.599PheThr: 1.599 ± 0.409
2.665PheVal: 2.665 ± 0.743
0.426PheTrp: 0.426 ± 0.181
1.706PheTyr: 1.706 ± 0.412
0.0PheXaa: 0.0 ± 0.0
Gly
3.305GlyAla: 3.305 ± 0.839
0.533GlyCys: 0.533 ± 0.257
5.437GlyAsp: 5.437 ± 0.657
3.945GlyGlu: 3.945 ± 0.603
2.132GlyPhe: 2.132 ± 0.481
3.198GlyGly: 3.198 ± 0.487
0.64GlyHis: 0.64 ± 0.252
4.691GlyIle: 4.691 ± 0.677
4.691GlyLys: 4.691 ± 0.542
4.691GlyLeu: 4.691 ± 0.712
1.279GlyMet: 1.279 ± 0.341
3.412GlyAsn: 3.412 ± 0.743
0.746GlyPro: 0.746 ± 0.257
2.452GlyGln: 2.452 ± 0.512
2.132GlyArg: 2.132 ± 0.462
3.092GlySer: 3.092 ± 0.483
3.625GlyThr: 3.625 ± 0.485
4.158GlyVal: 4.158 ± 0.699
1.173GlyTrp: 1.173 ± 0.561
2.985GlyTyr: 2.985 ± 0.614
0.0GlyXaa: 0.0 ± 0.0
His
0.533HisAla: 0.533 ± 0.232
0.32HisCys: 0.32 ± 0.155
0.959HisAsp: 0.959 ± 0.305
1.173HisGlu: 1.173 ± 0.346
1.386HisPhe: 1.386 ± 0.314
0.533HisGly: 0.533 ± 0.237
0.426HisHis: 0.426 ± 0.207
2.026HisIle: 2.026 ± 0.471
1.066HisLys: 1.066 ± 0.343
0.959HisLeu: 0.959 ± 0.302
0.533HisMet: 0.533 ± 0.199
0.853HisAsn: 0.853 ± 0.322
0.533HisPro: 0.533 ± 0.246
0.64HisGln: 0.64 ± 0.254
0.426HisArg: 0.426 ± 0.194
0.959HisSer: 0.959 ± 0.334
1.279HisThr: 1.279 ± 0.414
0.959HisVal: 0.959 ± 0.296
0.107HisTrp: 0.107 ± 0.095
0.426HisTyr: 0.426 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
6.29IleAla: 6.29 ± 0.8
0.426IleCys: 0.426 ± 0.253
4.691IleAsp: 4.691 ± 0.692
5.224IleGlu: 5.224 ± 0.857
2.878IlePhe: 2.878 ± 0.777
3.945IleGly: 3.945 ± 0.558
1.066IleHis: 1.066 ± 0.281
5.117IleIle: 5.117 ± 0.702
7.783IleLys: 7.783 ± 0.83
5.437IleLeu: 5.437 ± 0.811
1.706IleMet: 1.706 ± 0.312
3.838IleAsn: 3.838 ± 0.708
2.985IlePro: 2.985 ± 0.478
4.051IleGln: 4.051 ± 0.447
3.305IleArg: 3.305 ± 0.549
5.97IleSer: 5.97 ± 0.975
4.158IleThr: 4.158 ± 0.651
4.691IleVal: 4.691 ± 0.818
0.426IleTrp: 0.426 ± 0.232
1.812IleTyr: 1.812 ± 0.428
0.0IleXaa: 0.0 ± 0.0
Lys
5.757LysAla: 5.757 ± 0.783
0.107LysCys: 0.107 ± 0.091
6.397LysAsp: 6.397 ± 0.784
9.168LysGlu: 9.168 ± 1.228
2.665LysPhe: 2.665 ± 0.466
4.691LysGly: 4.691 ± 0.803
1.493LysHis: 1.493 ± 0.429
6.61LysIle: 6.61 ± 0.933
8.316LysLys: 8.316 ± 1.115
5.544LysLeu: 5.544 ± 0.613
1.919LysMet: 1.919 ± 0.603
4.797LysAsn: 4.797 ± 0.684
2.772LysPro: 2.772 ± 0.397
3.625LysGln: 3.625 ± 0.685
4.371LysArg: 4.371 ± 1.009
5.011LysSer: 5.011 ± 0.857
5.864LysThr: 5.864 ± 0.86
4.371LysVal: 4.371 ± 0.74
0.853LysTrp: 0.853 ± 0.245
1.919LysTyr: 1.919 ± 0.39
0.0LysXaa: 0.0 ± 0.0
Leu
6.93LeuAla: 6.93 ± 0.929
0.533LeuCys: 0.533 ± 0.278
5.011LeuAsp: 5.011 ± 0.504
7.676LeuGlu: 7.676 ± 1.267
4.158LeuPhe: 4.158 ± 0.707
5.65LeuGly: 5.65 ± 0.664
1.706LeuHis: 1.706 ± 0.39
4.371LeuIle: 4.371 ± 0.531
8.422LeuLys: 8.422 ± 0.923
5.011LeuLeu: 5.011 ± 0.715
1.919LeuMet: 1.919 ± 0.592
4.584LeuAsn: 4.584 ± 0.551
2.132LeuPro: 2.132 ± 0.413
3.198LeuGln: 3.198 ± 0.853
3.945LeuArg: 3.945 ± 0.704
4.584LeuSer: 4.584 ± 0.702
6.077LeuThr: 6.077 ± 0.651
3.625LeuVal: 3.625 ± 0.464
0.853LeuTrp: 0.853 ± 0.239
2.985LeuTyr: 2.985 ± 0.604
0.0LeuXaa: 0.0 ± 0.0
Met
1.706MetAla: 1.706 ± 0.425
0.107MetCys: 0.107 ± 0.118
1.706MetAsp: 1.706 ± 0.363
2.026MetGlu: 2.026 ± 0.473
1.279MetPhe: 1.279 ± 0.427
0.746MetGly: 0.746 ± 0.233
0.32MetHis: 0.32 ± 0.158
1.066MetIle: 1.066 ± 0.37
2.772MetLys: 2.772 ± 0.557
1.919MetLeu: 1.919 ± 0.553
1.066MetMet: 1.066 ± 0.349
2.132MetAsn: 2.132 ± 0.51
1.066MetPro: 1.066 ± 0.403
1.066MetGln: 1.066 ± 0.291
1.493MetArg: 1.493 ± 0.444
1.919MetSer: 1.919 ± 0.482
2.452MetThr: 2.452 ± 0.572
1.493MetVal: 1.493 ± 0.391
0.32MetTrp: 0.32 ± 0.221
0.32MetTyr: 0.32 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
4.051AsnAla: 4.051 ± 0.901
0.32AsnCys: 0.32 ± 0.187
2.878AsnAsp: 2.878 ± 0.627
4.264AsnGlu: 4.264 ± 0.634
2.559AsnPhe: 2.559 ± 0.563
4.371AsnGly: 4.371 ± 0.646
1.493AsnHis: 1.493 ± 0.42
3.092AsnIle: 3.092 ± 0.546
5.117AsnLys: 5.117 ± 0.555
4.797AsnLeu: 4.797 ± 0.855
1.066AsnMet: 1.066 ± 0.447
2.878AsnAsn: 2.878 ± 0.71
2.772AsnPro: 2.772 ± 0.472
3.518AsnGln: 3.518 ± 0.643
1.706AsnArg: 1.706 ± 0.412
2.452AsnSer: 2.452 ± 0.482
3.092AsnThr: 3.092 ± 0.442
3.838AsnVal: 3.838 ± 0.874
0.853AsnTrp: 0.853 ± 0.303
2.026AsnTyr: 2.026 ± 0.621
0.0AsnXaa: 0.0 ± 0.0
Pro
1.812ProAla: 1.812 ± 0.575
0.32ProCys: 0.32 ± 0.177
2.026ProAsp: 2.026 ± 0.584
2.132ProGlu: 2.132 ± 0.482
0.959ProPhe: 0.959 ± 0.296
1.066ProGly: 1.066 ± 0.302
0.426ProHis: 0.426 ± 0.201
2.452ProIle: 2.452 ± 0.505
1.706ProLys: 1.706 ± 0.464
2.665ProLeu: 2.665 ± 0.503
0.32ProMet: 0.32 ± 0.175
0.959ProAsn: 0.959 ± 0.347
0.533ProPro: 0.533 ± 0.214
1.599ProGln: 1.599 ± 0.412
0.746ProArg: 0.746 ± 0.287
2.026ProSer: 2.026 ± 0.42
2.239ProThr: 2.239 ± 0.398
2.026ProVal: 2.026 ± 0.397
0.426ProTrp: 0.426 ± 0.195
1.706ProTyr: 1.706 ± 0.496
0.0ProXaa: 0.0 ± 0.0
Gln
5.33GlnAla: 5.33 ± 1.06
0.0GlnCys: 0.0 ± 0.0
1.599GlnAsp: 1.599 ± 0.407
3.625GlnGlu: 3.625 ± 0.54
1.919GlnPhe: 1.919 ± 0.49
1.812GlnGly: 1.812 ± 0.437
0.426GlnHis: 0.426 ± 0.232
3.305GlnIle: 3.305 ± 0.658
3.945GlnLys: 3.945 ± 0.598
2.878GlnLeu: 2.878 ± 0.665
1.706GlnMet: 1.706 ± 0.515
2.772GlnAsn: 2.772 ± 0.629
1.386GlnPro: 1.386 ± 0.401
1.919GlnGln: 1.919 ± 0.488
1.599GlnArg: 1.599 ± 0.471
2.878GlnSer: 2.878 ± 0.567
2.132GlnThr: 2.132 ± 0.369
2.452GlnVal: 2.452 ± 0.497
0.32GlnTrp: 0.32 ± 0.176
1.173GlnTyr: 1.173 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
2.239ArgAla: 2.239 ± 0.514
0.107ArgCys: 0.107 ± 0.096
1.706ArgAsp: 1.706 ± 0.533
3.945ArgGlu: 3.945 ± 0.676
1.173ArgPhe: 1.173 ± 0.349
2.026ArgGly: 2.026 ± 0.466
0.426ArgHis: 0.426 ± 0.211
2.452ArgIle: 2.452 ± 0.448
3.412ArgLys: 3.412 ± 0.678
4.158ArgLeu: 4.158 ± 0.549
1.066ArgMet: 1.066 ± 0.413
3.731ArgAsn: 3.731 ± 0.422
1.279ArgPro: 1.279 ± 0.414
1.493ArgGln: 1.493 ± 0.296
2.452ArgArg: 2.452 ± 0.58
2.132ArgSer: 2.132 ± 0.505
2.026ArgThr: 2.026 ± 0.603
2.132ArgVal: 2.132 ± 0.41
1.279ArgTrp: 1.279 ± 0.47
3.198ArgTyr: 3.198 ± 0.661
0.0ArgXaa: 0.0 ± 0.0
Ser
3.625SerAla: 3.625 ± 0.777
0.107SerCys: 0.107 ± 0.095
4.371SerAsp: 4.371 ± 0.77
4.904SerGlu: 4.904 ± 0.78
3.625SerPhe: 3.625 ± 0.657
4.691SerGly: 4.691 ± 0.819
0.746SerHis: 0.746 ± 0.321
3.305SerIle: 3.305 ± 0.45
3.945SerLys: 3.945 ± 0.7
5.117SerLeu: 5.117 ± 0.533
1.919SerMet: 1.919 ± 0.578
3.412SerAsn: 3.412 ± 0.714
1.493SerPro: 1.493 ± 0.413
2.132SerGln: 2.132 ± 0.539
2.878SerArg: 2.878 ± 0.428
2.878SerSer: 2.878 ± 0.531
2.878SerThr: 2.878 ± 0.473
2.772SerVal: 2.772 ± 0.464
0.853SerTrp: 0.853 ± 0.28
2.559SerTyr: 2.559 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
3.945ThrAla: 3.945 ± 0.645
0.426ThrCys: 0.426 ± 0.235
3.198ThrAsp: 3.198 ± 0.71
4.264ThrGlu: 4.264 ± 0.584
2.345ThrPhe: 2.345 ± 0.645
3.518ThrGly: 3.518 ± 0.692
1.279ThrHis: 1.279 ± 0.355
5.33ThrIle: 5.33 ± 0.993
4.158ThrLys: 4.158 ± 0.683
5.011ThrLeu: 5.011 ± 0.64
1.279ThrMet: 1.279 ± 0.355
3.412ThrAsn: 3.412 ± 0.541
1.173ThrPro: 1.173 ± 0.364
2.878ThrGln: 2.878 ± 0.75
3.092ThrArg: 3.092 ± 0.602
3.412ThrSer: 3.412 ± 0.534
3.518ThrThr: 3.518 ± 0.646
4.478ThrVal: 4.478 ± 0.886
0.746ThrTrp: 0.746 ± 0.276
1.599ThrTyr: 1.599 ± 0.41
0.0ThrXaa: 0.0 ± 0.0
Val
3.092ValAla: 3.092 ± 0.749
0.107ValCys: 0.107 ± 0.104
3.092ValAsp: 3.092 ± 0.592
5.65ValGlu: 5.65 ± 0.817
1.706ValPhe: 1.706 ± 0.408
4.371ValGly: 4.371 ± 0.662
1.173ValHis: 1.173 ± 0.393
4.051ValIle: 4.051 ± 0.616
5.117ValLys: 5.117 ± 0.691
4.584ValLeu: 4.584 ± 0.691
1.493ValMet: 1.493 ± 0.386
3.625ValAsn: 3.625 ± 0.596
1.386ValPro: 1.386 ± 0.407
1.599ValGln: 1.599 ± 0.403
2.132ValArg: 2.132 ± 0.616
5.117ValSer: 5.117 ± 0.834
5.011ValThr: 5.011 ± 0.784
4.904ValVal: 4.904 ± 0.664
0.746ValTrp: 0.746 ± 0.392
0.959ValTyr: 0.959 ± 0.359
0.0ValXaa: 0.0 ± 0.0
Trp
0.533TrpAla: 0.533 ± 0.232
0.107TrpCys: 0.107 ± 0.096
0.533TrpAsp: 0.533 ± 0.216
1.173TrpGlu: 1.173 ± 0.347
0.853TrpPhe: 0.853 ± 0.401
0.64TrpGly: 0.64 ± 0.216
0.213TrpHis: 0.213 ± 0.167
1.066TrpIle: 1.066 ± 0.382
1.066TrpLys: 1.066 ± 0.397
0.959TrpLeu: 0.959 ± 0.244
0.32TrpMet: 0.32 ± 0.152
1.386TrpAsn: 1.386 ± 0.312
0.426TrpPro: 0.426 ± 0.218
0.853TrpGln: 0.853 ± 0.276
0.64TrpArg: 0.64 ± 0.249
0.746TrpSer: 0.746 ± 0.259
0.426TrpThr: 0.426 ± 0.162
0.426TrpVal: 0.426 ± 0.197
0.32TrpTrp: 0.32 ± 0.232
0.64TrpTyr: 0.64 ± 0.445
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.493TyrAla: 1.493 ± 0.421
0.746TyrCys: 0.746 ± 0.335
2.239TyrAsp: 2.239 ± 0.362
3.518TyrGlu: 3.518 ± 0.728
2.026TyrPhe: 2.026 ± 0.594
1.812TyrGly: 1.812 ± 0.389
0.533TyrHis: 0.533 ± 0.199
1.812TyrIle: 1.812 ± 0.553
2.878TyrLys: 2.878 ± 0.621
3.731TyrLeu: 3.731 ± 0.521
0.853TyrMet: 0.853 ± 0.302
1.493TyrAsn: 1.493 ± 0.484
1.919TyrPro: 1.919 ± 0.452
1.279TyrGln: 1.279 ± 0.359
2.239TyrArg: 2.239 ± 0.422
2.239TyrSer: 2.239 ± 0.541
1.173TyrThr: 1.173 ± 0.33
2.239TyrVal: 2.239 ± 0.423
0.533TyrTrp: 0.533 ± 0.203
1.812TyrTyr: 1.812 ± 0.665
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (9381 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski