Paenibacillus psychroresistens
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6938 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B8RJZ1|A0A6B8RJZ1_9BACL Ferredoxin--NADP reductase OS=Paenibacillus psychroresistens OX=1778678 GN=EHS13_14015 PE=3 SV=1
MM1 pKa = 7.79 SIQNLIVNGGFEE13 pKa = 4.6 TGTLAPWIPANTTITSLFSHH33 pKa = 6.68 SGTFSARR40 pKa = 11.84 LQGGPVTSYY49 pKa = 9.85 IGQFVPVTQGDD60 pKa = 3.83 SFEE63 pKa = 4.5 ILISLAKK70 pKa = 10.64 VGFLPAAPVIIQVFYY85 pKa = 10.71 FDD87 pKa = 5.91 SIFNLLGQGLFTNVPIDD104 pKa = 5.04 RR105 pKa = 11.84 IPTADD110 pKa = 2.99 NHH112 pKa = 5.42 TWLEE116 pKa = 4.25 IYY118 pKa = 8.26 QTTTPAPLGSTQAFVLIDD136 pKa = 3.86 NLPQAGTADD145 pKa = 3.39 ILVDD149 pKa = 4.8 DD150 pKa = 4.7 VALLAAGGSGSTGPTGATGATGDD173 pKa = 3.71 TGAMGVTGATGAMGVAGVTGAMGVTGVTGAMGVTGVTGVTGATGDD218 pKa = 3.7 TGVTGATGDD227 pKa = 3.7 TGVTGATGDD236 pKa = 3.7 TGVTGATGDD245 pKa = 3.7 TGVTGATGDD254 pKa = 3.7 TGVTGATGDD263 pKa = 3.58 TGATGATGATGDD275 pKa = 3.96 TGAMGATGVTGVTGATGDD293 pKa = 3.58 TGATGATGTTGATGVTGSTGATGDD317 pKa = 3.39 TGATGTTGVTGATGAMGVTGATGTTGATGDD347 pKa = 3.54 TGATGTTGTTGTTGVTGATGTTGVTGATGAMGVTGATGTTGATGVTGATGDD398 pKa = 3.48 TGATGVTGTTGVTGTTGVTGVTGVTGVTGATGATGATGATGTTGATGATGATGATGVTGATGATGATGAMGVTGVTGTTGATGDD482 pKa = 3.73 TGATGATGATGVTGATGDD500 pKa = 3.58 TGATGATGATGVTGATGATGVTGATGATGATGATGATGATGATGATGATGAIGDD554 pKa = 4.16 TGVTGATGATGAVSSAYY571 pKa = 10.11 AYY573 pKa = 10.35 VFNLGVQGVGANVDD587 pKa = 3.87 VIFSNNGPLVGGISHH602 pKa = 7.61 IAGTAPVTILVAGNYY617 pKa = 8.76 HH618 pKa = 5.85 IQWSITVNQNIQTAYY633 pKa = 10.62 AIVLNGATLPQTLFGFGVNSSANSFFQTAGHH664 pKa = 6.5 SIITVPASATITLRR678 pKa = 11.84 NVGFADD684 pKa = 3.75 NLATSLDD691 pKa = 3.51 GQQIVNASLEE701 pKa = 4.41 IIKK704 pKa = 9.02 LTLL707 pKa = 3.39
Molecular weight: 64.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.592
IPC_protein 3.63
Toseland 3.389
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.656
Rodwell 3.452
Grimsley 3.3
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 4.101
Thurlkill 3.465
EMBOSS 3.656
Sillero 3.77
Patrickios 1.036
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A6B8RUQ9|A0A6B8RUQ9_9BACL N-acetyltransferase OS=Paenibacillus psychroresistens OX=1778678 GN=EHS13_33140 PE=4 SV=1
MM1 pKa = 7.67 EE2 pKa = 4.37 PTFRR6 pKa = 11.84 PNVRR10 pKa = 11.84 KK11 pKa = 9.86 RR12 pKa = 11.84 KK13 pKa = 8.61 KK14 pKa = 8.79 NHH16 pKa = 4.94 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MSTKK25 pKa = 9.13 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VIQARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.52 GRR39 pKa = 11.84 KK40 pKa = 9.07 KK41 pKa = 9.61 LTAA44 pKa = 4.2
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6938
0
6938
2366348
25
6114
341.1
38.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.501 ± 0.036
0.738 ± 0.009
5.109 ± 0.022
6.248 ± 0.037
4.471 ± 0.023
6.767 ± 0.038
2.041 ± 0.015
7.82 ± 0.032
5.988 ± 0.03
10.069 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.675 ± 0.016
4.674 ± 0.03
3.809 ± 0.018
3.976 ± 0.022
3.975 ± 0.024
6.668 ± 0.027
5.817 ± 0.046
6.611 ± 0.024
1.334 ± 0.012
3.709 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here