Sulfolobus virus Ragged Hills

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus; Sulfolobus spindle-shaped virus 8

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6TRW3|Q6TRW3_9VIRU ORF A83 OS=Sulfolobus virus Ragged Hills OX=256994 PE=4 SV=1
MM1 pKa = 7.22MSALGDD7 pKa = 3.49VIYY10 pKa = 10.44ILGILIPLLGLVVRR24 pKa = 11.84NYY26 pKa = 10.63LVEE29 pKa = 3.99LLGFVMGTIAFLAFVQNYY47 pKa = 8.54TDD49 pKa = 3.26ITFSASNFYY58 pKa = 10.7LALLPLAFGLMDD70 pKa = 3.9FAFFFNWLKK79 pKa = 10.53EE80 pKa = 3.93EE81 pKa = 4.61RR82 pKa = 11.84II83 pKa = 3.96

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6TRV3|Q6TRV3_9VIRU ORF E152 OS=Sulfolobus virus Ragged Hills OX=256994 PE=4 SV=1
MM1 pKa = 7.35LTQLYY6 pKa = 9.82FSEE9 pKa = 4.45SKK11 pKa = 11.19LMVNSVFKK19 pKa = 11.05LLTLRR24 pKa = 11.84QKK26 pKa = 10.69LPKK29 pKa = 10.21NPEE32 pKa = 3.75SQITLNSYY40 pKa = 10.84YY41 pKa = 11.04LLTFQKK47 pKa = 10.35SIPPQTLTKK56 pKa = 9.74IKK58 pKa = 10.57SVNTLLTLFPLILLTIPILFRR79 pKa = 11.84RR80 pKa = 11.84LSQFSVFTLFLTQLVLVKK98 pKa = 10.55VSEE101 pKa = 4.05VRR103 pKa = 11.84KK104 pKa = 9.71NGRR107 pKa = 11.84SFRR110 pKa = 3.89

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

5372

49

812

145.2

16.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.914 ± 0.262

0.633 ± 0.215

3.388 ± 0.49

6.515 ± 1.084

4.691 ± 0.321

5.715 ± 0.629

1.247 ± 0.257

8.004 ± 0.466

7.893 ± 1.086

11.616 ± 0.531

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.159 ± 0.31

5.156 ± 0.613

3.593 ± 0.352

3.071 ± 0.363

3.779 ± 0.614

6.794 ± 0.732

6.385 ± 0.759

7.204 ± 0.482

1.247 ± 0.199

5.994 ± 0.484
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski