Frog adenovirus 1 (strain ATCC VR-896) (FrAdV-1)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9IIG5|Q9IIG5_ADEF1 ORF7 OS=Frog adenovirus 1 (strain ATCC VR-896) OX=114102 PE=4 SV=1
MM1 pKa = 6.31 SWYY4 pKa = 9.74 RR5 pKa = 11.84 VRR7 pKa = 11.84 YY8 pKa = 8.87 VDD10 pKa = 4.83 EE11 pKa = 4.78 NEE13 pKa = 4.0 EE14 pKa = 4.45 DD15 pKa = 3.85 PPSLHH20 pKa = 6.8 EE21 pKa = 5.16 LFDD24 pKa = 4.63 LEE26 pKa = 4.36 PTSPVQEE33 pKa = 4.2 NAVSSSQPFYY43 pKa = 11.07 SGRR46 pKa = 11.84 KK47 pKa = 8.6 YY48 pKa = 10.59 DD49 pKa = 4.18 IPPCMSEE56 pKa = 3.78 HH57 pKa = 5.77 QFISVYY63 pKa = 10.2 QKK65 pKa = 10.67 PGDD68 pKa = 3.89 DD69 pKa = 3.48 EE70 pKa = 6.0 DD71 pKa = 4.46 YY72 pKa = 11.76 VFIEE76 pKa = 4.26 NSGKK80 pKa = 9.57 MKK82 pKa = 10.38 LSEE85 pKa = 4.62 YY86 pKa = 9.38 NFSWAFFHH94 pKa = 7.41 SGVDD98 pKa = 3.22 QMIIYY103 pKa = 10.1 FPDD106 pKa = 3.05 WFAKK110 pKa = 9.94 LFKK113 pKa = 10.52 EE114 pKa = 4.96 FIYY117 pKa = 10.49 HH118 pKa = 7.26 KK119 pKa = 10.33 FRR121 pKa = 11.84 NDD123 pKa = 2.88 TEE125 pKa = 4.02 LQYY128 pKa = 10.84 QIEE131 pKa = 4.44 SEE133 pKa = 4.16 DD134 pKa = 4.5 GITKK138 pKa = 10.03 ILFTDD143 pKa = 3.47 SRR145 pKa = 11.84 SWPPVLACFVWQCSANGLDD164 pKa = 3.95 FFEE167 pKa = 4.49 LVSWDD172 pKa = 3.54 STDD175 pKa = 5.06 AEE177 pKa = 4.09 IQKK180 pKa = 8.56 TVFAA184 pKa = 5.47
Molecular weight: 21.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.221
IPC2_protein 4.355
IPC_protein 4.291
Toseland 4.126
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.139
Rodwell 4.139
Grimsley 4.037
Solomon 4.253
Lehninger 4.202
Nozaki 4.368
DTASelect 4.546
Thurlkill 4.139
EMBOSS 4.164
Sillero 4.418
Patrickios 3.032
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.32
Protein with the highest isoelectric point:
>tr|Q9IIH8|Q9IIH8_ADEF1 PVII OS=Frog adenovirus 1 (strain ATCC VR-896) OX=114102 PE=3 SV=1
MM1 pKa = 7.55 FEE3 pKa = 3.97 NLAPRR8 pKa = 11.84 KK9 pKa = 9.92 GITQIRR15 pKa = 11.84 PKK17 pKa = 10.25 ISYY20 pKa = 9.95 NKK22 pKa = 8.83 EE23 pKa = 3.55 LRR25 pKa = 11.84 GGFLPLLVPIIAAAISAAPGIAGAVLAAKK54 pKa = 9.74 NHH56 pKa = 5.2 NN57 pKa = 3.69
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.809
IPC_protein 10.277
Toseland 10.847
ProMoST 10.394
Dawson 10.921
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.389
Grimsley 10.95
Solomon 11.008
Lehninger 10.994
Nozaki 10.804
DTASelect 10.555
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 11.199
IPC_peptide 11.023
IPC2_peptide 9.107
IPC2.peptide.svr19 8.655
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
8125
57
1108
353.3
39.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.785 ± 0.476
1.908 ± 0.365
4.812 ± 0.246
5.858 ± 0.559
4.985 ± 0.27
5.354 ± 0.559
1.883 ± 0.225
5.969 ± 0.39
5.932 ± 0.729
9.772 ± 0.661
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.363 ± 0.184
6.486 ± 0.421
5.342 ± 0.27
4.086 ± 0.299
4.517 ± 0.439
7.569 ± 0.405
6.178 ± 0.374
6.166 ± 0.491
1.268 ± 0.151
3.766 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here