Corynebacterium sp. ATCC 6931
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A088QJ25|A0A088QJ25_9CORY OsmC-like family protein OS=Corynebacterium sp. ATCC 6931 OX=1487956 GN=DR71_1732 PE=4 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 KK3 pKa = 9.53 LPTAHH8 pKa = 6.98 CVTPTNTHH16 pKa = 5.21 HH17 pKa = 6.58 TFASRR22 pKa = 11.84 RR23 pKa = 11.84 ALIAGILATTALTFTACSQNDD44 pKa = 4.09 GAVTSQSAADD54 pKa = 3.94 TATMTQTDD62 pKa = 4.19 GNASNANSEE71 pKa = 4.07 NRR73 pKa = 11.84 PNPTGSNSDD82 pKa = 3.14 AGGSNNGGAAGNSDD96 pKa = 4.29 DD97 pKa = 4.8 TSDD100 pKa = 4.32 DD101 pKa = 3.89 ANSSEE106 pKa = 4.46 TAPSQNGGQTNGDD119 pKa = 3.81 TEE121 pKa = 4.75 TTSSSSSEE129 pKa = 3.94 NADD132 pKa = 2.48 IGQAMRR138 pKa = 11.84 DD139 pKa = 3.45 IIYY142 pKa = 10.47 SNSSPGTTFTGGEE155 pKa = 4.12 EE156 pKa = 4.05 MTVCVYY162 pKa = 11.38 GDD164 pKa = 4.01 GYY166 pKa = 9.72 RR167 pKa = 11.84 TNLVVAGPNTSCEE180 pKa = 3.81 FATEE184 pKa = 4.22 VFHH187 pKa = 6.97 QQTDD191 pKa = 3.95 GLNATADD198 pKa = 3.93 NIRR201 pKa = 11.84 DD202 pKa = 3.71 NLKK205 pKa = 10.64 PNIQATSPATGQTYY219 pKa = 10.42 DD220 pKa = 3.98 VSCGTQGDD228 pKa = 4.35 GVVACSGGNDD238 pKa = 2.79 ARR240 pKa = 11.84 IYY242 pKa = 10.78 INN244 pKa = 3.6
Molecular weight: 24.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.859
IPC2_protein 4.113
IPC_protein 4.101
Toseland 3.884
ProMoST 4.266
Dawson 4.101
Bjellqvist 4.253
Wikipedia 4.037
Rodwell 3.923
Grimsley 3.795
Solomon 4.088
Lehninger 4.05
Nozaki 4.202
DTASelect 4.469
Thurlkill 3.935
EMBOSS 4.05
Sillero 4.228
Patrickios 1.977
IPC_peptide 4.088
IPC2_peptide 4.202
IPC2.peptide.svr19 4.102
Protein with the highest isoelectric point:
>tr|A0A088QHD9|A0A088QHD9_9CORY Putative membrane protein OS=Corynebacterium sp. ATCC 6931 OX=1487956 GN=DR71_1210 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 VHH17 pKa = 5.99 GFRR20 pKa = 11.84 TRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 SGRR29 pKa = 11.84 AVVAARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.67 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.676
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.398
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2105
0
2105
715380
30
3034
339.8
36.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.684 ± 0.062
0.731 ± 0.014
6.189 ± 0.042
6.491 ± 0.055
3.25 ± 0.029
8.426 ± 0.05
2.027 ± 0.021
5.159 ± 0.036
3.456 ± 0.044
9.25 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.27 ± 0.025
2.967 ± 0.028
4.997 ± 0.034
3.189 ± 0.032
6.238 ± 0.048
6.102 ± 0.043
5.92 ± 0.037
8.164 ± 0.043
1.358 ± 0.022
2.133 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here