Diachasmimorpha longicaudata rhabdovirus
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0X9K747|A0A0X9K747_9RHAB Putative glycoprotein B OS=Diachasmimorpha longicaudata rhabdovirus OX=1585246 GN=G PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 6.3 LYY4 pKa = 9.21 NTIINGAKK12 pKa = 9.89 EE13 pKa = 3.61 IDD15 pKa = 3.45 IGGSNPMTGALSGWTDD31 pKa = 3.38 DD32 pKa = 5.05 SYY34 pKa = 11.73 KK35 pKa = 10.41 QLYY38 pKa = 7.86 GHH40 pKa = 6.46 VRR42 pKa = 11.84 ITQLEE47 pKa = 4.31 VSFIPRR53 pKa = 11.84 ILKK56 pKa = 9.9 AWFSTYY62 pKa = 10.54 YY63 pKa = 9.65 NHH65 pKa = 7.61 NIGNIDD71 pKa = 3.97 LSSLMMSTLSLITVSSVSKK90 pKa = 10.38 GQTSQTPNIQEE101 pKa = 4.17 MISIAEE107 pKa = 3.94 NLGKK111 pKa = 10.3 VEE113 pKa = 3.99 SQLRR117 pKa = 11.84 KK118 pKa = 9.53 NSPLIDD124 pKa = 4.01 HH125 pKa = 7.39 DD126 pKa = 4.53 SEE128 pKa = 5.86 KK129 pKa = 11.13 EE130 pKa = 3.85 DD131 pKa = 3.68 ILAGDD136 pKa = 4.26 HH137 pKa = 6.58 EE138 pKa = 4.84 EE139 pKa = 3.93 QHH141 pKa = 6.96 SIDD144 pKa = 4.17 SSDD147 pKa = 3.36 VMYY150 pKa = 11.07 LSMISDD156 pKa = 4.36 PDD158 pKa = 3.79 YY159 pKa = 11.24 QDD161 pKa = 3.12 TLINIASFQALVMLRR176 pKa = 11.84 HH177 pKa = 5.16 ITKK180 pKa = 9.92 TNTSVDD186 pKa = 3.06 KK187 pKa = 10.58 YY188 pKa = 10.55 QRR190 pKa = 11.84 EE191 pKa = 4.31 SLTRR195 pKa = 11.84 TLKK198 pKa = 10.64 SLCPNTLLTADD209 pKa = 4.63 IPPPSAQFVTYY220 pKa = 9.95 FKK222 pKa = 10.6 LNCMKK227 pKa = 10.7 GNDD230 pKa = 3.55 VATSLLAYY238 pKa = 10.38 VMNLYY243 pKa = 9.53 MEE245 pKa = 5.13 ARR247 pKa = 11.84 QSGATPLVDD256 pKa = 3.86 YY257 pKa = 11.24 LNAGCLTHH265 pKa = 7.25 LKK267 pKa = 10.86 GNGLALLEE275 pKa = 5.51 LIFQVHH281 pKa = 6.89 LCTKK285 pKa = 10.44 LKK287 pKa = 10.87 LSDD290 pKa = 4.47 LFDD293 pKa = 3.47 MTYY296 pKa = 10.88 SSQCAQSIDD305 pKa = 3.29 RR306 pKa = 11.84 AYY308 pKa = 11.02 HH309 pKa = 5.48 HH310 pKa = 6.52 FKK312 pKa = 10.57 EE313 pKa = 4.22 YY314 pKa = 11.4 AEE316 pKa = 4.6 LKK318 pKa = 10.84 SNDD321 pKa = 3.17 GKK323 pKa = 10.64 IIRR326 pKa = 11.84 PAQVTWWWARR336 pKa = 11.84 LFNDD340 pKa = 4.37 KK341 pKa = 11.04 YY342 pKa = 11.15 FMDD345 pKa = 3.58 MSIRR349 pKa = 11.84 NNYY352 pKa = 8.21 QYY354 pKa = 11.04 CLRR357 pKa = 11.84 LACILTCYY365 pKa = 8.33 QQQGSPLEE373 pKa = 4.01 AAGFGTVSAKK383 pKa = 10.16 EE384 pKa = 3.93 LSKK387 pKa = 11.42 HH388 pKa = 5.91 NDD390 pKa = 2.9 FATKK394 pKa = 9.9 FVAHH398 pKa = 6.61 LTPDD402 pKa = 3.2 EE403 pKa = 4.42 DD404 pKa = 4.09 KK405 pKa = 11.42 FMGGALKK412 pKa = 10.25 ILNTAVPGPSGTSRR426 pKa = 11.84 PPLDD430 pKa = 3.91 IEE432 pKa = 4.72 SEE434 pKa = 4.19
Molecular weight: 48.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.378
IPC2_protein 5.397
IPC_protein 5.41
Toseland 5.728
ProMoST 5.614
Dawson 5.601
Bjellqvist 5.664
Wikipedia 5.588
Rodwell 5.563
Grimsley 5.842
Solomon 5.601
Lehninger 5.588
Nozaki 5.842
DTASelect 6.033
Thurlkill 5.957
EMBOSS 5.931
Sillero 5.931
Patrickios 3.668
IPC_peptide 5.614
IPC2_peptide 5.931
IPC2.peptide.svr19 5.918
Protein with the highest isoelectric point:
>tr|A0A0X9JET7|A0A0X9JET7_9RHAB Uncharacterized protein OS=Diachasmimorpha longicaudata rhabdovirus OX=1585246 GN=RS08_02 PE=4 SV=1
MM1 pKa = 6.88 QQDD4 pKa = 2.97 KK5 pKa = 10.13 MGYY8 pKa = 9.07 SVSFTLKK15 pKa = 10.15 GHH17 pKa = 6.85 LKK19 pKa = 10.28 IINCPKK25 pKa = 9.66 KK26 pKa = 9.45 WLDD29 pKa = 3.47 FKK31 pKa = 11.85 YY32 pKa = 10.49 EE33 pKa = 4.17 LSCLILSHH41 pKa = 6.77 WEE43 pKa = 3.7 NDD45 pKa = 4.01 FPCLLINEE53 pKa = 5.18 EE54 pKa = 4.31 IRR56 pKa = 11.84 GLYY59 pKa = 10.29 YY60 pKa = 10.73 NVLVNLLEE68 pKa = 4.69 KK69 pKa = 9.79 KK70 pKa = 10.0 HH71 pKa = 6.33 VMIRR75 pKa = 11.84 DD76 pKa = 3.54 GVGGYY81 pKa = 10.04 DD82 pKa = 4.19 CYY84 pKa = 11.11 IDD86 pKa = 5.54 LISKK90 pKa = 9.96 GFLHH94 pKa = 6.99 KK95 pKa = 10.78 AMRR98 pKa = 11.84 QPVMTSVKK106 pKa = 9.49 QEE108 pKa = 3.73 SRR110 pKa = 11.84 ITSVSFPLTEE120 pKa = 4.1 DD121 pKa = 3.04 TTILVKK127 pKa = 10.78 GIIQLIQCPFKK138 pKa = 10.48 IDD140 pKa = 3.16 NWEE143 pKa = 3.85 KK144 pKa = 10.92 AVAQGYY150 pKa = 9.54 RR151 pKa = 11.84 PFNNMIKK158 pKa = 9.96 KK159 pKa = 10.57 DD160 pKa = 3.65 SGGKK164 pKa = 9.41 KK165 pKa = 9.49 NQVEE169 pKa = 4.19 LRR171 pKa = 11.84 SISSGPSTAVPEE183 pKa = 4.32 EE184 pKa = 3.96 IKK186 pKa = 10.65 IKK188 pKa = 10.53 NLLYY192 pKa = 10.83 NWIKK196 pKa = 10.71 KK197 pKa = 8.84 HH198 pKa = 5.6
Molecular weight: 22.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.583
IPC2_protein 8.448
IPC_protein 8.331
Toseland 9.004
ProMoST 8.858
Dawson 9.326
Bjellqvist 9.18
Wikipedia 9.443
Rodwell 9.575
Grimsley 9.297
Solomon 9.399
Lehninger 9.37
Nozaki 9.37
DTASelect 9.063
Thurlkill 9.224
EMBOSS 9.487
Sillero 9.385
Patrickios 4.711
IPC_peptide 9.399
IPC2_peptide 7.849
IPC2.peptide.svr19 7.715
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3971
198
2110
661.8
75.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.651 ± 0.6
1.939 ± 0.089
4.684 ± 0.357
5.691 ± 0.645
4.18 ± 0.231
5.162 ± 0.139
2.871 ± 0.256
7.63 ± 0.21
6.573 ± 0.728
10.677 ± 1.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.846 ± 0.226
4.81 ± 0.217
4.911 ± 0.216
3.803 ± 0.221
4.13 ± 0.273
9.62 ± 0.47
5.993 ± 0.64
5.288 ± 0.55
1.637 ± 0.278
3.903 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here