Mycoplana dimorpha
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4217 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T5BB28|A0A2T5BB28_MYCDI 2-oxoisovalerate dehydrogenase subunit alpha OS=Mycoplana dimorpha OX=28320 GN=C7449_103191 PE=3 SV=1
MM1 pKa = 7.48 SMKK4 pKa = 10.41 FLTSTALLALVAAPALAADD23 pKa = 3.74 VVSYY27 pKa = 9.41 QEE29 pKa = 4.11 PAPQVEE35 pKa = 4.35 ASPAFSWTGGYY46 pKa = 10.0 VGLQGGGAWLNGDD59 pKa = 3.97 FSIPGASASEE69 pKa = 4.17 DD70 pKa = 3.69 FNGGLIGGFAGYY82 pKa = 10.53 NFQQGNWVFGVEE94 pKa = 4.59 GDD96 pKa = 4.75 VSYY99 pKa = 11.55 NWNDD103 pKa = 2.96 NNYY106 pKa = 10.61 DD107 pKa = 3.32 IFGTTAEE114 pKa = 4.28 VGTDD118 pKa = 3.03 VSGSVRR124 pKa = 11.84 GRR126 pKa = 11.84 AGYY129 pKa = 10.84 AFDD132 pKa = 4.03 SALIYY137 pKa = 9.43 ATAGWTATRR146 pKa = 11.84 GFVDD150 pKa = 3.65 VPGFDD155 pKa = 4.23 KK156 pKa = 10.38 EE157 pKa = 4.7 TEE159 pKa = 4.26 VFNGWTIGAGADD171 pKa = 3.31 YY172 pKa = 11.21 AFTNNIFGRR181 pKa = 11.84 AEE183 pKa = 3.47 YY184 pKa = 9.99 RR185 pKa = 11.84 YY186 pKa = 10.58 NDD188 pKa = 4.14 FGDD191 pKa = 3.8 KK192 pKa = 10.41 DD193 pKa = 3.62 IQGVNVDD200 pKa = 4.41 LDD202 pKa = 3.57 QHH204 pKa = 5.84 QVTLGIGVKK213 pKa = 9.91 FF214 pKa = 3.75
Molecular weight: 22.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.732
EMBOSS 3.846
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A2T5AXF0|A0A2T5AXF0_MYCDI Molybdenum import ATP-binding protein ModC OS=Mycoplana dimorpha OX=28320 GN=modC PE=3 SV=1
MM1 pKa = 7.8 RR2 pKa = 11.84 PFALSKK8 pKa = 10.53 AIRR11 pKa = 11.84 PLALAATASLVAACQSSAPAPQASTAALPTMEE43 pKa = 5.7 RR44 pKa = 11.84 IALGANACWFKK55 pKa = 11.33 SGDD58 pKa = 3.66 AAFQAYY64 pKa = 9.5 RR65 pKa = 11.84 LAPEE69 pKa = 4.69 LNSFSGKK76 pKa = 9.23 PRR78 pKa = 11.84 ILVVPRR84 pKa = 11.84 RR85 pKa = 11.84 SPEE88 pKa = 3.69 ARR90 pKa = 11.84 PLLVVQAEE98 pKa = 4.12 GHH100 pKa = 5.96 PARR103 pKa = 11.84 LSAFGPLMDD112 pKa = 4.63 EE113 pKa = 4.29 PVSGRR118 pKa = 11.84 IAADD122 pKa = 3.38 VKK124 pKa = 10.16 RR125 pKa = 11.84 WAAGGAGCRR134 pKa = 3.99
Molecular weight: 13.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.428
IPC_protein 10.131
Toseland 10.687
ProMoST 10.833
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.891
Grimsley 10.774
Solomon 10.906
Lehninger 10.877
Nozaki 10.701
DTASelect 10.452
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.701
Patrickios 10.687
IPC_peptide 10.921
IPC2_peptide 9.794
IPC2.peptide.svr19 8.834
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4217
0
4217
1319512
26
2827
312.9
33.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.453 ± 0.05
0.832 ± 0.014
5.633 ± 0.029
5.832 ± 0.034
3.843 ± 0.02
8.524 ± 0.037
2.032 ± 0.019
5.379 ± 0.027
3.4 ± 0.031
10.004 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.017
2.662 ± 0.022
4.999 ± 0.027
3.024 ± 0.023
7.07 ± 0.041
5.425 ± 0.023
5.236 ± 0.028
7.514 ± 0.03
1.28 ± 0.016
2.306 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here