Aphanothece sacrum FPU1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Aphanothece; Aphanothece sacrum

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401IC74|A0A401IC74_APHSA Ferredoxin-nitrite reductase OS=Aphanothece sacrum FPU1 OX=1920663 GN=AsFPU1_0239 PE=4 SV=1
MM1 pKa = 7.53AVLEE5 pKa = 4.19QAFLLSQTTLQNFANNPDD23 pKa = 3.83FAAKK27 pKa = 8.85MALAFNSTSQADD39 pKa = 4.02TLQTAWLNGDD49 pKa = 3.59FAGFPALEE57 pKa = 3.68IRR59 pKa = 11.84YY60 pKa = 9.29QSEE63 pKa = 4.21LNGANGAYY71 pKa = 10.17SADD74 pKa = 3.2TGIIYY79 pKa = 10.38LAYY82 pKa = 9.87EE83 pKa = 4.18YY84 pKa = 10.71LLQLFPPSQGGLGGINALVGLILEE108 pKa = 4.7EE109 pKa = 4.13YY110 pKa = 8.63GHH112 pKa = 6.07YY113 pKa = 10.62VDD115 pKa = 5.83VVLNGSNDD123 pKa = 3.36SDD125 pKa = 3.88GDD127 pKa = 3.47EE128 pKa = 4.21GAIFSALVMGQSLSEE143 pKa = 4.07TDD145 pKa = 3.44LGQLKK150 pKa = 9.08TEE152 pKa = 4.3NDD154 pKa = 3.23HH155 pKa = 7.1GVISFNGVNIAVEE168 pKa = 4.22MQNFAGSAGNDD179 pKa = 3.9TITGTSGDD187 pKa = 4.16DD188 pKa = 3.78VIDD191 pKa = 4.02GLGGNDD197 pKa = 4.15SLNGLAGDD205 pKa = 5.25DD206 pKa = 4.02ILNGGDD212 pKa = 4.55GNDD215 pKa = 3.94TLDD218 pKa = 4.22GGDD221 pKa = 4.54GNDD224 pKa = 3.63ILNPGLGVDD233 pKa = 4.5TIDD236 pKa = 4.52GNTGTDD242 pKa = 3.51TLIADD247 pKa = 3.89YY248 pKa = 7.79TTATVSGIGTNNYY261 pKa = 10.21GITNTSSKK269 pKa = 10.07IYY271 pKa = 10.22SSYY274 pKa = 11.61SSTTLLNYY282 pKa = 10.81SNIEE286 pKa = 3.94LFNITGTQYY295 pKa = 11.54NDD297 pKa = 3.08SFSANTGYY305 pKa = 10.68KK306 pKa = 9.5INAGGGSDD314 pKa = 3.77TLTLSLSNTSTNWVLDD330 pKa = 3.51WANANAQLSGDD341 pKa = 4.06SNTQISNFEE350 pKa = 4.61KK351 pKa = 10.26ISSVTTGSGNDD362 pKa = 3.2TFKK365 pKa = 10.53FTNYY369 pKa = 10.16LLNSSIDD376 pKa = 3.72GNTGTDD382 pKa = 3.48TLIADD387 pKa = 4.29CTTATVSGIGTNNYY401 pKa = 10.21GITNTSSKK409 pKa = 10.07IYY411 pKa = 10.22SSYY414 pKa = 11.61SSTTLLNYY422 pKa = 10.81SNIEE426 pKa = 3.94LFNITGTQYY435 pKa = 11.23NDD437 pKa = 3.16SLTGYY442 pKa = 9.91AGNDD446 pKa = 3.64TLIGAAGDD454 pKa = 3.63DD455 pKa = 4.55TINGTIGDD463 pKa = 4.35SLDD466 pKa = 3.71GGEE469 pKa = 4.55GTDD472 pKa = 3.87TVNLDD477 pKa = 3.78LSSAMTNLTFDD488 pKa = 4.68LDD490 pKa = 4.24LSVTTNLQGISGTEE504 pKa = 3.5VKK506 pKa = 10.61NFEE509 pKa = 4.63KK510 pKa = 10.34IGTITTGSGNDD521 pKa = 3.2TFKK524 pKa = 10.5FTNYY528 pKa = 10.65LLGGTINGNTGTDD541 pKa = 3.56TLIADD546 pKa = 3.92YY547 pKa = 7.79TTATGSGISTNNYY560 pKa = 9.62GITNTSSRR568 pKa = 11.84IYY570 pKa = 10.53SSYY573 pKa = 11.49NSGTFLNYY581 pKa = 10.77SNIEE585 pKa = 4.02LFNITGTQYY594 pKa = 11.23NDD596 pKa = 3.16SLTGYY601 pKa = 9.91AGNDD605 pKa = 3.64TLIGAAGDD613 pKa = 3.63DD614 pKa = 4.55TINGTIGDD622 pKa = 4.43SLDD625 pKa = 3.63GGKK628 pKa = 8.37GTDD631 pKa = 3.56TVNLDD636 pKa = 3.53LSSAITNLTFDD647 pKa = 4.96LDD649 pKa = 4.24LSVTTNLQGISGTEE663 pKa = 3.5VKK665 pKa = 10.61NFEE668 pKa = 4.63KK669 pKa = 10.34IGTITTGSGNDD680 pKa = 3.2TFKK683 pKa = 10.5FTNYY687 pKa = 10.65LLGGTINGNTGTDD700 pKa = 3.56TLIADD705 pKa = 3.92YY706 pKa = 7.79TTATGSGISTNNYY719 pKa = 9.62GITNTSSRR727 pKa = 11.84IYY729 pKa = 10.53SSYY732 pKa = 11.49NSGTFLNYY740 pKa = 10.77SNIEE744 pKa = 4.02LFNITGTQYY753 pKa = 11.23NDD755 pKa = 3.16SLTGYY760 pKa = 9.91AGNDD764 pKa = 3.64TLIGAAGDD772 pKa = 3.63DD773 pKa = 4.55TINGTIGDD781 pKa = 4.43SLDD784 pKa = 3.63GGKK787 pKa = 8.37GTDD790 pKa = 3.56TVNLDD795 pKa = 3.53LSSAITNLTFDD806 pKa = 4.96LDD808 pKa = 4.24LSVTTNLQGISGTEE822 pKa = 3.5VKK824 pKa = 10.61NFEE827 pKa = 4.63KK828 pKa = 10.34IGTITTGSGNDD839 pKa = 3.2TFKK842 pKa = 10.5FTNYY846 pKa = 10.65LLGGTINGNTGTDD859 pKa = 3.56TLIADD864 pKa = 3.92YY865 pKa = 7.79TTATGSGISTNNYY878 pKa = 9.62GITNTSSKK886 pKa = 10.07IYY888 pKa = 10.22SSYY891 pKa = 11.61SSTTLLNYY899 pKa = 10.81SNIEE903 pKa = 3.94LFNITGTQYY912 pKa = 11.53NDD914 pKa = 3.22NLNGSGGNDD923 pKa = 3.3TLIGGEE929 pKa = 4.47GNDD932 pKa = 4.04SLSGGDD938 pKa = 4.31GNDD941 pKa = 3.26KK942 pKa = 11.01LSGVNAKK949 pKa = 10.35KK950 pKa = 9.09LTPGLNEE957 pKa = 4.03IDD959 pKa = 3.8TLSGGNSIDD968 pKa = 3.63TFILGDD974 pKa = 3.67AANIYY979 pKa = 10.81YY980 pKa = 10.08DD981 pKa = 4.16DD982 pKa = 4.79RR983 pKa = 11.84NNTTTGTTDD992 pKa = 3.17YY993 pKa = 11.78ARR995 pKa = 11.84ITDD998 pKa = 4.48FNATQDD1004 pKa = 3.71IIQLNGAKK1012 pKa = 10.02SNYY1015 pKa = 9.71RR1016 pKa = 11.84LTTASFNNISGTAIYY1031 pKa = 10.35VDD1033 pKa = 4.14KK1034 pKa = 10.97PNTEE1038 pKa = 3.95PDD1040 pKa = 3.33EE1041 pKa = 4.77LIGFIEE1047 pKa = 4.93GVTGLDD1053 pKa = 3.05INSNAFVTAKK1063 pKa = 10.97DD1064 pKa = 3.7EE1065 pKa = 4.34IAFSNAQFRR1074 pKa = 11.84VIEE1077 pKa = 4.8DD1078 pKa = 3.57GTPVVAVTVVRR1089 pKa = 11.84SGAVQTEE1096 pKa = 4.06VSATITLNNGTAISPEE1112 pKa = 4.47DD1113 pKa = 3.9YY1114 pKa = 10.63NNTPIIVNFAQGEE1127 pKa = 4.28TSQTVIIPIIDD1138 pKa = 3.8DD1139 pKa = 3.99SEE1141 pKa = 4.7FEE1143 pKa = 4.18LDD1145 pKa = 3.29EE1146 pKa = 4.86TINLTLTNPTNGAVLGSQNTASLTIVDD1173 pKa = 4.59NEE1175 pKa = 4.09IPLPGILAFSATNYY1189 pKa = 10.58SITEE1193 pKa = 4.41DD1194 pKa = 3.38GTPIVTVTIIRR1205 pKa = 11.84TDD1207 pKa = 3.41GSDD1210 pKa = 3.46GAVSATLNLSDD1221 pKa = 5.04DD1222 pKa = 3.93TATAPNDD1229 pKa = 3.59YY1230 pKa = 10.33GNTPIVVNFAHH1241 pKa = 7.46RR1242 pKa = 11.84EE1243 pKa = 3.79TSKK1246 pKa = 9.93TVTIPIVNDD1255 pKa = 3.82SIYY1258 pKa = 11.04EE1259 pKa = 4.0GDD1261 pKa = 3.65EE1262 pKa = 4.05TLKK1265 pKa = 11.15LSLTNPTGGATLGGQNTANLTIVDD1289 pKa = 4.14NDD1291 pKa = 3.94LPTISLVVSPDD1302 pKa = 3.1SVTEE1306 pKa = 4.35DD1307 pKa = 3.13GLTNLVYY1314 pKa = 10.42TFIRR1318 pKa = 11.84TGNTVSPLTVNFNVGGTGIFNNDD1341 pKa = 3.79YY1342 pKa = 9.27IQSGAASFNGTSGSITFAAGSDD1364 pKa = 3.76TAILTLDD1371 pKa = 3.56PTVDD1375 pKa = 3.33SMFEE1379 pKa = 3.69ADD1381 pKa = 3.42EE1382 pKa = 4.48TVNLTLVSSANYY1394 pKa = 10.02NRR1396 pKa = 11.84GTTTAVNSIITNDD1409 pKa = 4.65DD1410 pKa = 3.32INSGTFSFSSPQFTVNEE1427 pKa = 4.57DD1428 pKa = 3.49GTPITAVTINRR1439 pKa = 11.84AGNSNGEE1446 pKa = 4.02VSVTINLNNGTATAPNDD1463 pKa = 3.76YY1464 pKa = 9.79NNSPIVVTFASGQQTKK1480 pKa = 7.92TVNIPIQNDD1489 pKa = 3.3LTRR1492 pKa = 11.84EE1493 pKa = 3.89GDD1495 pKa = 3.29EE1496 pKa = 4.63SINMVLSNPTGGANLGTQTSANLIIADD1523 pKa = 3.91NDD1525 pKa = 3.13IGLNAEE1531 pKa = 4.12YY1532 pKa = 10.65FNGYY1536 pKa = 10.23FNDD1539 pKa = 3.41NLGFFTANQPILKK1552 pKa = 8.95RR1553 pKa = 11.84TDD1555 pKa = 2.93KK1556 pKa = 10.67TVNFLNLAWGHH1567 pKa = 5.35EE1568 pKa = 4.33TLKK1571 pKa = 11.02ALPDD1575 pKa = 3.91DD1576 pKa = 4.77EE1577 pKa = 6.11KK1578 pKa = 11.67SKK1580 pKa = 8.9TWW1582 pKa = 3.13

Molecular weight: 165.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401IKL5|A0A401IKL5_APHSA VWFA domain-containing protein OS=Aphanothece sacrum FPU1 OX=1920663 GN=AsFPU1_3140 PE=4 SV=1
MM1 pKa = 7.78IEE3 pKa = 4.04RR4 pKa = 11.84EE5 pKa = 4.23KK6 pKa = 10.96KK7 pKa = 9.86RR8 pKa = 11.84SRR10 pKa = 11.84LVDD13 pKa = 3.59KK14 pKa = 10.8YY15 pKa = 10.75AEE17 pKa = 3.97KK18 pKa = 10.6RR19 pKa = 11.84ATLKK23 pKa = 11.02EE24 pKa = 4.07EE25 pKa = 3.78FRR27 pKa = 11.84VAEE30 pKa = 4.53DD31 pKa = 4.44YY32 pKa = 10.56EE33 pKa = 5.33DD34 pKa = 3.73KK35 pKa = 11.02MNLQRR40 pKa = 11.84QLQQLPRR47 pKa = 11.84NSSPNRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84NRR57 pKa = 11.84CWVTGRR63 pKa = 11.84SRR65 pKa = 11.84GYY67 pKa = 10.25YY68 pKa = 9.53RR69 pKa = 11.84DD70 pKa = 3.76FGLSRR75 pKa = 11.84NVLRR79 pKa = 11.84EE80 pKa = 3.67WAHH83 pKa = 5.74QGLLPGVVKK92 pKa = 10.78SSWW95 pKa = 2.92

Molecular weight: 11.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4301

0

4301

1220765

39

3314

283.8

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.634 ± 0.041

0.992 ± 0.015

4.949 ± 0.036

6.53 ± 0.044

4.058 ± 0.029

6.57 ± 0.051

1.757 ± 0.021

7.763 ± 0.035

5.572 ± 0.042

11.128 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.917 ± 0.02

4.913 ± 0.044

4.563 ± 0.03

5.293 ± 0.036

4.521 ± 0.036

6.36 ± 0.033

5.757 ± 0.047

6.028 ± 0.032

1.413 ± 0.019

3.28 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski