Amino acid dipepetide frequency for Aphanothece sacrum FPU1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.108AlaAla: 5.108 ± 0.084	0.688AlaCys: 0.688 ± 0.028	3.128AlaAsp: 3.128 ± 0.057	4.057AlaGlu: 4.057 ± 0.062	2.584AlaPhe: 2.584 ± 0.052	4.346AlaGly: 4.346 ± 0.075	1.055AlaHis: 1.055 ± 0.032	6.004AlaIle: 6.004 ± 0.077	4.054AlaLys: 4.054 ± 0.072	7.185AlaLeu: 7.185 ± 0.085	1.5AlaMet: 1.5 ± 0.042	3.096AlaAsn: 3.096 ± 0.073	2.216AlaPro: 2.216 ± 0.048	3.761AlaGln: 3.761 ± 0.063	2.678AlaArg: 2.678 ± 0.051	3.796AlaSer: 3.796 ± 0.063	3.856AlaThr: 3.856 ± 0.068	4.151AlaVal: 4.151 ± 0.069	0.828AlaTrp: 0.828 ± 0.022	2.255AlaTyr: 2.255 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.517CysAla: 0.517 ± 0.023	0.154CysCys: 0.154 ± 0.011	0.53CysAsp: 0.53 ± 0.023	0.5CysGlu: 0.5 ± 0.021	0.434CysPhe: 0.434 ± 0.021	0.775CysGly: 0.775 ± 0.028	0.288CysHis: 0.288 ± 0.015	0.593CysIle: 0.593 ± 0.021	0.325CysLys: 0.325 ± 0.017	1.296CysLeu: 1.296 ± 0.036	0.152CysMet: 0.152 ± 0.011	0.347CysAsn: 0.347 ± 0.016	0.591CysPro: 0.591 ± 0.024	0.738CysGln: 0.738 ± 0.022	0.497CysArg: 0.497 ± 0.021	0.665CysSer: 0.665 ± 0.024	0.448CysThr: 0.448 ± 0.021	0.522CysVal: 0.522 ± 0.021	0.151CysTrp: 0.151 ± 0.012	0.396CysTyr: 0.396 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	2.941AspAla: 2.941 ± 0.069	0.548AspCys: 0.548 ± 0.021	2.215AspAsp: 2.215 ± 0.049	3.015AspGlu: 3.015 ± 0.06	2.335AspPhe: 2.335 ± 0.047	3.183AspGly: 3.183 ± 0.082	0.881AspHis: 0.881 ± 0.025	3.97AspIle: 3.97 ± 0.056	2.865AspLys: 2.865 ± 0.05	5.81AspLeu: 5.81 ± 0.07	0.799AspMet: 0.799 ± 0.028	2.644AspAsn: 2.644 ± 0.053	2.371AspPro: 2.371 ± 0.047	1.959AspGln: 1.959 ± 0.041	2.615AspArg: 2.615 ± 0.054	2.966AspSer: 2.966 ± 0.056	2.553AspThr: 2.553 ± 0.059	2.773AspVal: 2.773 ± 0.056	0.891AspTrp: 0.891 ± 0.03	2.154AspTyr: 2.154 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	4.808GluAla: 4.808 ± 0.079	0.472GluCys: 0.472 ± 0.021	3.115GluAsp: 3.115 ± 0.054	4.594GluGlu: 4.594 ± 0.09	2.489GluPhe: 2.489 ± 0.044	3.568GluGly: 3.568 ± 0.063	0.924GluHis: 0.924 ± 0.028	5.74GluIle: 5.74 ± 0.074	4.48GluLys: 4.48 ± 0.069	7.073GluLeu: 7.073 ± 0.09	1.415GluMet: 1.415 ± 0.034	3.248GluAsn: 3.248 ± 0.054	2.24GluPro: 2.24 ± 0.044	3.646GluGln: 3.646 ± 0.055	3.097GluArg: 3.097 ± 0.062	3.546GluSer: 3.546 ± 0.064	4.111GluThr: 4.111 ± 0.075	4.114GluVal: 4.114 ± 0.076	0.756GluTrp: 0.756 ± 0.027	1.869GluTyr: 1.869 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	2.677PheAla: 2.677 ± 0.056	0.556PheCys: 0.556 ± 0.021	2.34PheAsp: 2.34 ± 0.053	2.371PheGlu: 2.371 ± 0.05	1.858PhePhe: 1.858 ± 0.044	2.735PheGly: 2.735 ± 0.046	0.71PheHis: 0.71 ± 0.025	2.811PheIle: 2.811 ± 0.058	1.954PheLys: 1.954 ± 0.041	4.354PheLeu: 4.354 ± 0.078	0.768PheMet: 0.768 ± 0.029	2.103PheAsn: 2.103 ± 0.048	1.952PhePro: 1.952 ± 0.042	1.671PheGln: 1.671 ± 0.037	1.755PheArg: 1.755 ± 0.042	3.101PheSer: 3.101 ± 0.059	2.291PheThr: 2.291 ± 0.059	2.289PheVal: 2.289 ± 0.053	0.695PheTrp: 0.695 ± 0.026	1.584PheTyr: 1.584 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	3.973GlyAla: 3.973 ± 0.083	0.768GlyCys: 0.768 ± 0.027	3.318GlyAsp: 3.318 ± 0.075	4.083GlyGlu: 4.083 ± 0.067	2.953GlyPhe: 2.953 ± 0.049	4.708GlyGly: 4.708 ± 0.092	1.2GlyHis: 1.2 ± 0.032	5.646GlyIle: 5.646 ± 0.072	4.295GlyLys: 4.295 ± 0.063	7.029GlyLeu: 7.029 ± 0.103	1.518GlyMet: 1.518 ± 0.037	3.314GlyAsn: 3.314 ± 0.111	1.35GlyPro: 1.35 ± 0.034	3.11GlyGln: 3.11 ± 0.058	2.778GlyArg: 2.778 ± 0.06	3.73GlySer: 3.73 ± 0.063	3.962GlyThr: 3.962 ± 0.088	4.451GlyVal: 4.451 ± 0.08	1.075GlyTrp: 1.075 ± 0.034	2.442GlyTyr: 2.442 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	0.839HisAla: 0.839 ± 0.025	0.289HisCys: 0.289 ± 0.016	0.753HisAsp: 0.753 ± 0.032	0.88HisGlu: 0.88 ± 0.026	0.812HisPhe: 0.812 ± 0.027	1.065HisGly: 1.065 ± 0.03	0.668HisHis: 0.668 ± 0.03	1.098HisIle: 1.098 ± 0.032	0.802HisLys: 0.802 ± 0.026	2.231HisLeu: 2.231 ± 0.048	0.224HisMet: 0.224 ± 0.016	0.8HisAsn: 0.8 ± 0.024	1.266HisPro: 1.266 ± 0.031	1.153HisGln: 1.153 ± 0.029	1.0HisArg: 1.0 ± 0.03	1.103HisSer: 1.103 ± 0.036	0.831HisThr: 0.831 ± 0.026	0.68HisVal: 0.68 ± 0.025	0.347HisTrp: 0.347 ± 0.017	0.731HisTyr: 0.731 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.137IleAla: 6.137 ± 0.079	0.809IleCys: 0.809 ± 0.027	4.121IleAsp: 4.121 ± 0.069	5.376IleGlu: 5.376 ± 0.077	3.1IlePhe: 3.1 ± 0.058	4.807IleGly: 4.807 ± 0.07	1.249IleHis: 1.249 ± 0.038	6.022IleIle: 6.022 ± 0.095	4.401IleLys: 4.401 ± 0.072	8.162IleLeu: 8.162 ± 0.096	1.207IleMet: 1.207 ± 0.035	4.224IleAsn: 4.224 ± 0.072	4.132IlePro: 4.132 ± 0.059	3.022IleGln: 3.022 ± 0.051	3.275IleArg: 3.275 ± 0.052	5.171IleSer: 5.171 ± 0.068	4.509IleThr: 4.509 ± 0.075	4.463IleVal: 4.463 ± 0.055	0.975IleTrp: 0.975 ± 0.031	2.472IleTyr: 2.472 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	3.901LysAla: 3.901 ± 0.065	0.337LysCys: 0.337 ± 0.017	2.631LysAsp: 2.631 ± 0.043	3.833LysGlu: 3.833 ± 0.066	1.976LysPhe: 1.976 ± 0.048	3.158LysGly: 3.158 ± 0.056	0.83LysHis: 0.83 ± 0.025	4.906LysIle: 4.906 ± 0.078	3.214LysLys: 3.214 ± 0.062	6.037LysLeu: 6.037 ± 0.092	1.248LysMet: 1.248 ± 0.035	2.851LysAsn: 2.851 ± 0.059	2.514LysPro: 2.514 ± 0.053	3.096LysGln: 3.096 ± 0.052	2.577LysArg: 2.577 ± 0.051	3.317LysSer: 3.317 ± 0.055	3.512LysThr: 3.512 ± 0.06	3.448LysVal: 3.448 ± 0.053	0.627LysTrp: 0.627 ± 0.022	1.702LysTyr: 1.702 ± 0.044	0.0LysXaa: 0.0 ± 0.0
Leu	8.17LeuAla: 8.17 ± 0.095	1.049LeuCys: 1.049 ± 0.029	5.704LeuAsp: 5.704 ± 0.077	8.163LeuGlu: 8.163 ± 0.102	3.92LeuPhe: 3.92 ± 0.073	7.95LeuGly: 7.95 ± 0.093	1.667LeuHis: 1.667 ± 0.041	8.313LeuIle: 8.313 ± 0.105	6.74LeuLys: 6.74 ± 0.09	11.076LeuLeu: 11.076 ± 0.141	2.264LeuMet: 2.264 ± 0.044	5.254LeuAsn: 5.254 ± 0.074	5.275LeuPro: 5.275 ± 0.078	5.017LeuGln: 5.017 ± 0.086	4.798LeuArg: 4.798 ± 0.059	8.013LeuSer: 8.013 ± 0.098	7.043LeuThr: 7.043 ± 0.097	6.61LeuVal: 6.61 ± 0.086	1.395LeuTrp: 1.395 ± 0.04	2.863LeuTyr: 2.863 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.679MetAla: 1.679 ± 0.037	0.128MetCys: 0.128 ± 0.009	0.764MetAsp: 0.764 ± 0.025	1.069MetGlu: 1.069 ± 0.033	0.588MetPhe: 0.588 ± 0.029	1.548MetGly: 1.548 ± 0.038	0.231MetHis: 0.231 ± 0.016	1.506MetIle: 1.506 ± 0.031	1.161MetLys: 1.161 ± 0.032	1.833MetLeu: 1.833 ± 0.05	0.436MetMet: 0.436 ± 0.018	0.96MetAsn: 0.96 ± 0.033	0.812MetPro: 0.812 ± 0.027	0.668MetGln: 0.668 ± 0.022	0.839MetArg: 0.839 ± 0.025	1.484MetSer: 1.484 ± 0.041	1.578MetThr: 1.578 ± 0.036	1.345MetVal: 1.345 ± 0.037	0.147MetTrp: 0.147 ± 0.012	0.393MetTyr: 0.393 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.693AsnAla: 2.693 ± 0.058	0.575AsnCys: 0.575 ± 0.023	2.117AsnAsp: 2.117 ± 0.076	2.278AsnGlu: 2.278 ± 0.045	2.206AsnPhe: 2.206 ± 0.045	2.892AsnGly: 2.892 ± 0.086	0.99AsnHis: 0.99 ± 0.031	3.538AsnIle: 3.538 ± 0.073	2.436AsnLys: 2.436 ± 0.049	6.233AsnLeu: 6.233 ± 0.086	0.735AsnMet: 0.735 ± 0.023	2.982AsnAsn: 2.982 ± 0.082	3.159AsnPro: 3.159 ± 0.058	2.888AsnGln: 2.888 ± 0.054	2.333AsnArg: 2.333 ± 0.044	3.306AsnSer: 3.306 ± 0.062	2.657AsnThr: 2.657 ± 0.061	2.251AsnVal: 2.251 ± 0.045	0.85AsnTrp: 0.85 ± 0.025	2.015AsnTyr: 2.015 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	2.217ProAla: 2.217 ± 0.047	0.378ProCys: 0.378 ± 0.017	2.862ProAsp: 2.862 ± 0.051	3.615ProGlu: 3.615 ± 0.061	1.88ProPhe: 1.88 ± 0.039	2.724ProGly: 2.724 ± 0.049	0.917ProHis: 0.917 ± 0.028	3.578ProIle: 3.578 ± 0.061	2.296ProLys: 2.296 ± 0.052	4.949ProLeu: 4.949 ± 0.067	0.784ProMet: 0.784 ± 0.024	2.507ProAsn: 2.507 ± 0.046	2.13ProPro: 2.13 ± 0.059	2.597ProGln: 2.597 ± 0.05	1.615ProArg: 1.615 ± 0.035	3.033ProSer: 3.033 ± 0.054	2.668ProThr: 2.668 ± 0.056	2.831ProVal: 2.831 ± 0.047	0.584ProTrp: 0.584 ± 0.026	1.465ProTyr: 1.465 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	3.781GlnAla: 3.781 ± 0.065	0.365GlnCys: 0.365 ± 0.019	2.192GlnAsp: 2.192 ± 0.041	4.262GlnGlu: 4.262 ± 0.08	1.867GlnPhe: 1.867 ± 0.038	3.599GlnGly: 3.599 ± 0.056	0.682GlnHis: 0.682 ± 0.024	3.982GlnIle: 3.982 ± 0.05	3.344GlnLys: 3.344 ± 0.06	5.974GlnLeu: 5.974 ± 0.09	1.023GlnMet: 1.023 ± 0.025	2.15GlnAsn: 2.15 ± 0.04	2.162GlnPro: 2.162 ± 0.044	3.572GlnGln: 3.572 ± 0.067	2.538GlnArg: 2.538 ± 0.059	2.84GlnSer: 2.84 ± 0.057	3.056GlnThr: 3.056 ± 0.057	3.427GlnVal: 3.427 ± 0.052	0.779GlnTrp: 0.779 ± 0.025	1.339GlnTyr: 1.339 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.484ArgAla: 2.484 ± 0.051	0.453ArgCys: 0.453 ± 0.02	2.226ArgAsp: 2.226 ± 0.039	3.004ArgGlu: 3.004 ± 0.053	2.082ArgPhe: 2.082 ± 0.044	2.73ArgGly: 2.73 ± 0.051	0.908ArgHis: 0.908 ± 0.027	3.214ArgIle: 3.214 ± 0.058	2.258ArgLys: 2.258 ± 0.048	5.504ArgLeu: 5.504 ± 0.083	0.883ArgMet: 0.883 ± 0.027	1.918ArgAsn: 1.918 ± 0.039	1.832ArgPro: 1.832 ± 0.043	3.122ArgGln: 3.122 ± 0.063	2.528ArgArg: 2.528 ± 0.058	2.693ArgSer: 2.693 ± 0.047	2.051ArgThr: 2.051 ± 0.044	2.842ArgVal: 2.842 ± 0.056	0.732ArgTrp: 0.732 ± 0.027	1.742ArgTyr: 1.742 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	3.436SerAla: 3.436 ± 0.056	0.665SerCys: 0.665 ± 0.024	3.255SerAsp: 3.255 ± 0.054	3.721SerGlu: 3.721 ± 0.057	2.689SerPhe: 2.689 ± 0.056	4.284SerGly: 4.284 ± 0.071	1.378SerHis: 1.378 ± 0.034	4.361SerIle: 4.361 ± 0.067	2.827SerLys: 2.827 ± 0.053	7.99SerLeu: 7.99 ± 0.108	1.266SerMet: 1.266 ± 0.033	2.861SerAsn: 2.861 ± 0.061	3.608SerPro: 3.608 ± 0.061	4.044SerGln: 4.044 ± 0.067	2.86SerArg: 2.86 ± 0.051	4.514SerSer: 4.514 ± 0.074	3.164SerThr: 3.164 ± 0.054	3.714SerVal: 3.714 ± 0.054	0.958SerTrp: 0.958 ± 0.029	2.0SerTyr: 2.0 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	4.043ThrAla: 4.043 ± 0.08	0.481ThrCys: 0.481 ± 0.023	2.796ThrAsp: 2.796 ± 0.056	3.504ThrGlu: 3.504 ± 0.062	2.29ThrPhe: 2.29 ± 0.057	4.247ThrGly: 4.247 ± 0.089	1.01ThrHis: 1.01 ± 0.031	4.614ThrIle: 4.614 ± 0.086	2.579ThrLys: 2.579 ± 0.045	6.718ThrLeu: 6.718 ± 0.092	0.887ThrMet: 0.887 ± 0.027	2.576ThrAsn: 2.576 ± 0.061	3.421ThrPro: 3.421 ± 0.068	3.075ThrGln: 3.075 ± 0.056	2.134ThrArg: 2.134 ± 0.047	3.401ThrSer: 3.401 ± 0.069	3.323ThrThr: 3.323 ± 0.066	3.967ThrVal: 3.967 ± 0.075	0.721ThrTrp: 0.721 ± 0.024	1.784ThrTyr: 1.784 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.43ValAla: 4.43 ± 0.075	0.639ValCys: 0.639 ± 0.026	3.146ValAsp: 3.146 ± 0.06	4.038ValGlu: 4.038 ± 0.063	2.348ValPhe: 2.348 ± 0.051	4.112ValGly: 4.112 ± 0.083	0.899ValHis: 0.899 ± 0.03	4.86ValIle: 4.86 ± 0.065	3.619ValLys: 3.619 ± 0.065	5.79ValLeu: 5.79 ± 0.077	1.315ValMet: 1.315 ± 0.032	3.256ValAsn: 3.256 ± 0.075	2.533ValPro: 2.533 ± 0.046	2.261ValGln: 2.261 ± 0.041	2.624ValArg: 2.624 ± 0.054	4.105ValSer: 4.105 ± 0.063	3.763ValThr: 3.763 ± 0.057	3.909ValVal: 3.909 ± 0.061	0.768ValTrp: 0.768 ± 0.029	1.869ValTyr: 1.869 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	0.688TrpAla: 0.688 ± 0.024	0.152TrpCys: 0.152 ± 0.011	0.661TrpAsp: 0.661 ± 0.024	0.968TrpGlu: 0.968 ± 0.033	0.651TrpPhe: 0.651 ± 0.028	1.014TrpGly: 1.014 ± 0.03	0.31TrpHis: 0.31 ± 0.016	0.954TrpIle: 0.954 ± 0.027	0.695TrpLys: 0.695 ± 0.023	1.978TrpLeu: 1.978 ± 0.05	0.306TrpMet: 0.306 ± 0.016	0.577TrpAsn: 0.577 ± 0.025	0.302TrpPro: 0.302 ± 0.016	1.171TrpGln: 1.171 ± 0.037	0.713TrpArg: 0.713 ± 0.024	0.782TrpSer: 0.782 ± 0.028	0.646TrpThr: 0.646 ± 0.024	0.884TrpVal: 0.884 ± 0.027	0.238TrpTrp: 0.238 ± 0.014	0.442TrpTyr: 0.442 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.82TyrAla: 1.82 ± 0.038	0.415TyrCys: 0.415 ± 0.019	1.617TyrAsp: 1.617 ± 0.039	1.974TyrGlu: 1.974 ± 0.045	1.513TyrPhe: 1.513 ± 0.035	2.255TyrGly: 2.255 ± 0.045	0.777TyrHis: 0.777 ± 0.031	1.926TyrIle: 1.926 ± 0.044	1.37TyrLys: 1.37 ± 0.034	4.061TyrLeu: 4.061 ± 0.069	0.438TyrMet: 0.438 ± 0.021	1.465TyrAsn: 1.465 ± 0.04	1.762TyrPro: 1.762 ± 0.039	2.362TyrGln: 2.362 ± 0.05	1.952TyrArg: 1.952 ± 0.042	2.032TyrSer: 2.032 ± 0.041	1.546TyrThr: 1.546 ± 0.04	1.615TyrVal: 1.615 ± 0.04	0.618TyrTrp: 0.618 ± 0.024	1.28TyrTyr: 1.28 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4301 proteins (1220765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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