Salmonella phage SAP012

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4EFD2|A0A6J4EFD2_9CAUD Uncharacterized protein OS=Salmonella phage SAP012 OX=2742114 PE=4 SV=1
MM1 pKa = 7.21LTIKK5 pKa = 10.39DD6 pKa = 3.91LHH8 pKa = 6.24NAWKK12 pKa = 10.48SNTAAVIDD20 pKa = 4.3NPADD24 pKa = 2.81SWGRR28 pKa = 11.84YY29 pKa = 6.89TEE31 pKa = 4.18IACYY35 pKa = 9.56EE36 pKa = 4.14YY37 pKa = 11.62NDD39 pKa = 3.53ITKK42 pKa = 9.81LQYY45 pKa = 10.05HH46 pKa = 6.9HH47 pKa = 7.24CPDD50 pKa = 3.64YY51 pKa = 11.62NDD53 pKa = 3.72IEE55 pKa = 4.23EE56 pKa = 4.48KK57 pKa = 10.88GFNEE61 pKa = 4.16NMSNEE66 pKa = 3.88IAVLLGLMGVTFEE79 pKa = 5.08CIRR82 pKa = 11.84DD83 pKa = 3.12IDD85 pKa = 3.63RR86 pKa = 11.84RR87 pKa = 11.84MTTVIFRR94 pKa = 11.84RR95 pKa = 11.84GGVFYY100 pKa = 10.78AHH102 pKa = 7.74DD103 pKa = 4.77YY104 pKa = 10.79INVVNDD110 pKa = 3.08ISISDD115 pKa = 3.98ALGDD119 pKa = 3.64ALSRR123 pKa = 11.84CEE125 pKa = 4.13SFVVGDD131 pKa = 4.45PKK133 pKa = 10.94RR134 pKa = 11.84EE135 pKa = 4.08DD136 pKa = 3.6YY137 pKa = 9.17EE138 pKa = 4.12TEE140 pKa = 3.97YY141 pKa = 10.95QFKK144 pKa = 10.65KK145 pKa = 10.38EE146 pKa = 3.75YY147 pKa = 10.3RR148 pKa = 11.84EE149 pKa = 3.89YY150 pKa = 10.56CLLYY154 pKa = 10.71AVFTPGEE161 pKa = 4.15MLDD164 pKa = 4.43LLALVQSEE172 pKa = 4.2

Molecular weight: 19.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4EGZ9|A0A6J4EGZ9_9CAUD Uncharacterized protein OS=Salmonella phage SAP012 OX=2742114 PE=4 SV=1
MM1 pKa = 7.4ASKK4 pKa = 10.44RR5 pKa = 11.84RR6 pKa = 11.84IRR8 pKa = 11.84FKK10 pKa = 10.59EE11 pKa = 3.91CGRR14 pKa = 11.84KK15 pKa = 8.39QRR17 pKa = 11.84FPDD20 pKa = 3.38KK21 pKa = 10.07EE22 pKa = 4.01AASTAIQTLHH32 pKa = 6.76RR33 pKa = 11.84RR34 pKa = 11.84LGYY37 pKa = 10.18QGFICPYY44 pKa = 7.79PCRR47 pKa = 11.84WCNGWHH53 pKa = 6.31FGHH56 pKa = 6.95VKK58 pKa = 10.6GQDD61 pKa = 3.35TRR63 pKa = 11.84NKK65 pKa = 10.21

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

18347

47

1454

254.8

28.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.582 ± 0.569

0.981 ± 0.126

6.628 ± 0.238

6.17 ± 0.255

3.407 ± 0.157

7.364 ± 0.244

1.755 ± 0.135

5.559 ± 0.162

5.521 ± 0.294

7.304 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.178 ± 0.13

4.404 ± 0.19

4.578 ± 0.354

3.652 ± 0.298

5.663 ± 0.206

5.701 ± 0.273

6.192 ± 0.301

7.233 ± 0.365

1.728 ± 0.146

3.401 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski