Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 152 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O10335|TLP20_NPVOP Telokin-like protein 20 homolog OS=Orgyia pseudotsugata multicapsid polyhedrosis virus OX=262177 GN=ORF85 PE=4 SV=1
MM1 pKa = 7.14 NLDD4 pKa = 3.13 VPYY7 pKa = 10.42 YY8 pKa = 10.41 RR9 pKa = 11.84 LGAHH13 pKa = 6.0 EE14 pKa = 4.31 RR15 pKa = 11.84 VEE17 pKa = 4.27 YY18 pKa = 10.52 IPLKK22 pKa = 10.66 LALNDD27 pKa = 4.21 DD28 pKa = 4.57 APPPAAHH35 pKa = 7.2 EE36 pKa = 4.15 YY37 pKa = 11.07 AEE39 pKa = 4.07 ARR41 pKa = 11.84 AAGGEE46 pKa = 3.98 AAPPAEE52 pKa = 4.15 QWSAGVIVLIGLVAFVALFLLLYY75 pKa = 9.49 VIYY78 pKa = 10.48 YY79 pKa = 8.06 FVILRR84 pKa = 11.84 DD85 pKa = 3.55 QPQYY89 pKa = 11.65 SDD91 pKa = 6.01 DD92 pKa = 4.14 IDD94 pKa = 4.1 NDD96 pKa = 3.75 PPFAFNKK103 pKa = 9.81 FDD105 pKa = 3.29
Molecular weight: 11.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.958
IPC2_protein 4.24
IPC_protein 4.151
Toseland 3.961
ProMoST 4.202
Dawson 4.126
Bjellqvist 4.342
Wikipedia 4.062
Rodwell 3.986
Grimsley 3.872
Solomon 4.113
Lehninger 4.075
Nozaki 4.253
DTASelect 4.457
Thurlkill 3.999
EMBOSS 4.062
Sillero 4.266
Patrickios 0.947
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.198
Protein with the highest isoelectric point:
>sp|O10333|VP41_NPVOP Structural glycoprotein gp41 OS=Orgyia pseudotsugata multicapsid polyhedrosis virus OX=262177 GN=GP41 PE=2 SV=1
MM1 pKa = 7.32 SLYY4 pKa = 10.09 RR5 pKa = 11.84 SKK7 pKa = 10.62 VWCVYY12 pKa = 9.61 IVRR15 pKa = 11.84 RR16 pKa = 11.84 DD17 pKa = 3.59 DD18 pKa = 3.55 GQLYY22 pKa = 9.53 TGITSDD28 pKa = 3.33 LSRR31 pKa = 11.84 RR32 pKa = 11.84 LGEE35 pKa = 3.82 HH36 pKa = 5.75 ARR38 pKa = 11.84 GVGARR43 pKa = 11.84 CLRR46 pKa = 11.84 GAKK49 pKa = 9.65 RR50 pKa = 11.84 LQLLYY55 pKa = 10.38 CSASAYY61 pKa = 8.86 DD62 pKa = 4.32 HH63 pKa = 6.26 KK64 pKa = 9.85 TAAQMEE70 pKa = 4.47 YY71 pKa = 10.08 HH72 pKa = 7.24 LKK74 pKa = 10.29 RR75 pKa = 11.84 KK76 pKa = 8.71 RR77 pKa = 11.84 GKK79 pKa = 9.25 YY80 pKa = 9.82 FKK82 pKa = 10.73 LRR84 pKa = 11.84 LIKK87 pKa = 10.01 AQPRR91 pKa = 11.84 FLHH94 pKa = 6.15 QYY96 pKa = 10.63 LSADD100 pKa = 3.22 KK101 pKa = 10.33 PLRR104 pKa = 3.97
Molecular weight: 12.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.897
IPC_protein 10.54
Toseland 10.467
ProMoST 10.321
Dawson 10.643
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 10.935
Grimsley 10.716
Solomon 10.701
Lehninger 10.657
Nozaki 10.467
DTASelect 10.35
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.555
Patrickios 10.613
IPC_peptide 10.701
IPC2_peptide 9.385
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
152
0
152
39323
51
1223
258.7
29.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.567 ± 0.244
2.546 ± 0.146
6.017 ± 0.152
4.987 ± 0.137
4.72 ± 0.141
4.394 ± 0.165
2.566 ± 0.113
4.12 ± 0.119
4.979 ± 0.219
9.643 ± 0.178
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.077
5.544 ± 0.189
4.56 ± 0.258
3.843 ± 0.208
6.632 ± 0.222
5.676 ± 0.141
5.501 ± 0.161
7.53 ± 0.182
0.829 ± 0.067
3.883 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here