Sinomicrobium oceani
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4212 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1K1QBR9|A0A1K1QBR9_9FLAO Alpha-L-fucosidase OS=Sinomicrobium oceani OX=1150368 GN=SAMN02927921_02443 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.37 KK3 pKa = 9.66 ILALVLVSALVFVSCEE19 pKa = 3.93 GDD21 pKa = 3.18 QGPPGQDD28 pKa = 3.1 GLDD31 pKa = 3.67 GLNGHH36 pKa = 6.57 IFEE39 pKa = 5.59 AGPVDD44 pKa = 4.99 LSYY47 pKa = 11.61 DD48 pKa = 3.65 PATNLYY54 pKa = 10.23 SAIINFADD62 pKa = 3.55 EE63 pKa = 4.29 TDD65 pKa = 4.37 FEE67 pKa = 4.64 ILDD70 pKa = 3.63 TDD72 pKa = 3.52 AVLVYY77 pKa = 10.08 RR78 pKa = 11.84 LQGSVEE84 pKa = 4.27 FNDD87 pKa = 3.74 GTTADD92 pKa = 3.59 SWALLPLTYY101 pKa = 10.33 TPDD104 pKa = 3.2 EE105 pKa = 4.05 GTFYY109 pKa = 10.83 YY110 pKa = 10.28 TFEE113 pKa = 4.37 HH114 pKa = 6.78 NYY116 pKa = 9.17 IDD118 pKa = 3.58 TEE120 pKa = 4.06 IFIDD124 pKa = 4.52 GNFDD128 pKa = 3.63 LSGLASEE135 pKa = 4.57 YY136 pKa = 10.18 TQDD139 pKa = 3.19 QIFRR143 pKa = 11.84 IVIIPNPDD151 pKa = 3.12 AQGLNVSNFDD161 pKa = 3.77 EE162 pKa = 4.34 VLKK165 pKa = 10.9 ALNPEE170 pKa = 4.02 QIKK173 pKa = 10.56 QIEE176 pKa = 4.37 IKK178 pKa = 10.72 NN179 pKa = 3.68
Molecular weight: 19.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.694
IPC_protein 3.694
Toseland 3.478
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.516
Grimsley 3.389
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.541
EMBOSS 3.643
Sillero 3.821
Patrickios 0.985
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A1K1QG68|A0A1K1QG68_9FLAO Phosphate-selective porin O and P OS=Sinomicrobium oceani OX=1150368 GN=SAMN02927921_02506 PE=4 SV=1
MM1 pKa = 7.0 LTADD5 pKa = 3.87 RR6 pKa = 11.84 VMLTADD12 pKa = 3.71 RR13 pKa = 11.84 LMLTANRR20 pKa = 11.84 TGLTAKK26 pKa = 10.18 RR27 pKa = 11.84 VMSTANRR34 pKa = 11.84 VVLTAKK40 pKa = 10.4 RR41 pKa = 11.84 VGLTANRR48 pKa = 11.84 IGLTANRR55 pKa = 11.84 VVLTANRR62 pKa = 11.84 AMLPANRR69 pKa = 11.84 VMPTAKK75 pKa = 10.05 RR76 pKa = 11.84 VGLTANRR83 pKa = 11.84 IVLTANRR90 pKa = 11.84 VVLTANRR97 pKa = 11.84 VVLTANRR104 pKa = 11.84 VVLTANRR111 pKa = 11.84 VVLTANRR118 pKa = 11.84 VGLTANRR125 pKa = 11.84 VVLTANRR132 pKa = 11.84 IGLTANRR139 pKa = 11.84 VVLTANRR146 pKa = 11.84 VRR148 pKa = 11.84 LTVNRR153 pKa = 11.84 IGLTANRR160 pKa = 11.84 IRR162 pKa = 11.84 LTANRR167 pKa = 11.84 TGLVVNTIPP176 pKa = 4.74
Molecular weight: 19.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.498
IPC2_protein 11.111
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.442
Grimsley 12.925
Solomon 13.378
Lehninger 13.29
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.393
Sillero 12.881
Patrickios 12.149
IPC_peptide 13.393
IPC2_peptide 12.384
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4212
0
4212
1479298
21
3749
351.2
39.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.901 ± 0.033
0.746 ± 0.011
5.704 ± 0.026
6.735 ± 0.039
4.834 ± 0.028
7.162 ± 0.038
2.021 ± 0.02
6.973 ± 0.033
6.531 ± 0.048
9.127 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.354 ± 0.018
5.077 ± 0.037
3.844 ± 0.021
3.3 ± 0.02
4.719 ± 0.029
6.12 ± 0.031
5.846 ± 0.031
6.462 ± 0.029
1.201 ± 0.014
4.343 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here