Streptococcus satellite phage Javan293
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIV7|A0A4D5ZIV7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan293 OX=2558612 GN=JavanS293_0013 PE=4 SV=1
MM1 pKa = 7.39 LVTYY5 pKa = 8.27 PALFYY10 pKa = 11.3 YY11 pKa = 10.68 DD12 pKa = 4.09 DD13 pKa = 4.06 TNGTNVPYY21 pKa = 9.91 FVHH24 pKa = 7.0 FPDD27 pKa = 3.95 IDD29 pKa = 3.78 GSGTQGTSLSDD40 pKa = 3.47 AMAMASEE47 pKa = 4.29 YY48 pKa = 10.57 LGIMVAYY55 pKa = 9.47 YY56 pKa = 9.98 IEE58 pKa = 4.7 SNRR61 pKa = 11.84 EE62 pKa = 3.61 LPKK65 pKa = 10.48 PSEE68 pKa = 4.16 INNLSLMEE76 pKa = 4.53 NNPFKK81 pKa = 10.86 DD82 pKa = 3.62 EE83 pKa = 4.59 PDD85 pKa = 3.58 FLSYY89 pKa = 11.23 DD90 pKa = 3.51 PGKK93 pKa = 10.83 SFVSLVMVDD102 pKa = 3.19 VAEE105 pKa = 4.16 YY106 pKa = 10.54 LGSQEE111 pKa = 4.64 PIKK114 pKa = 10.26 KK115 pKa = 8.15 TLTIPRR121 pKa = 11.84 WADD124 pKa = 3.04 KK125 pKa = 10.79 LGRR128 pKa = 11.84 EE129 pKa = 4.06 LGLNFSQTLTEE140 pKa = 5.14 AIADD144 pKa = 3.94 KK145 pKa = 10.63 KK146 pKa = 10.1 IQAA149 pKa = 4.07
Molecular weight: 16.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.304
IPC2_protein 4.355
IPC_protein 4.266
Toseland 4.088
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.126
Rodwell 4.101
Grimsley 3.999
Solomon 4.228
Lehninger 4.177
Nozaki 4.342
DTASelect 4.533
Thurlkill 4.113
EMBOSS 4.139
Sillero 4.38
Patrickios 3.401
IPC_peptide 4.228
IPC2_peptide 4.368
IPC2.peptide.svr19 4.312
Protein with the highest isoelectric point:
>tr|A0A4D5ZJD1|A0A4D5ZJD1_9VIRU Mobile element protein OS=Streptococcus satellite phage Javan293 OX=2558612 GN=JavanS293_0009 PE=4 SV=1
MM1 pKa = 7.38 IMKK4 pKa = 8.9 ITEE7 pKa = 4.09 VKK9 pKa = 10.48 KK10 pKa = 10.14 KK11 pKa = 10.37 DD12 pKa = 3.24 GSIVYY17 pKa = 9.61 RR18 pKa = 11.84 ANIYY22 pKa = 10.71 LGVDD26 pKa = 3.6 NITGKK31 pKa = 10.25 KK32 pKa = 9.88 VKK34 pKa = 9.83 TSVTGRR40 pKa = 11.84 TKK42 pKa = 10.86 KK43 pKa = 10.07 EE44 pKa = 3.87 VSRR47 pKa = 11.84 KK48 pKa = 9.1 AQNAQLDD55 pKa = 3.98 FKK57 pKa = 11.24 INGSTVYY64 pKa = 9.03 KK65 pKa = 9.41 TVQIEE70 pKa = 4.63 SYY72 pKa = 10.83 KK73 pKa = 10.62 EE74 pKa = 3.74 LAEE77 pKa = 4.3 LWLEE81 pKa = 4.29 SYY83 pKa = 10.96 KK84 pKa = 10.44 LTVKK88 pKa = 10.09 PQTYY92 pKa = 10.46 RR93 pKa = 11.84 NTTLFLKK100 pKa = 10.7 NHH102 pKa = 7.14 LLPMFGDD109 pKa = 3.45 MRR111 pKa = 11.84 LEE113 pKa = 4.46 KK114 pKa = 9.33 ITPHH118 pKa = 5.27 IVQRR122 pKa = 11.84 FINRR126 pKa = 11.84 LAVTLANFRR135 pKa = 11.84 VPVSINSRR143 pKa = 11.84 ILQYY147 pKa = 11.38 AVTLQLIPYY156 pKa = 8.72 NPARR160 pKa = 11.84 DD161 pKa = 3.78 VILPPKK167 pKa = 8.79 PAKK170 pKa = 10.29 DD171 pKa = 3.41 PQKK174 pKa = 9.74 ITFIDD179 pKa = 3.62 KK180 pKa = 10.63 QDD182 pKa = 3.55 LKK184 pKa = 11.4 KK185 pKa = 11.04 LLDD188 pKa = 3.84 YY189 pKa = 11.1 VEE191 pKa = 5.15 KK192 pKa = 10.71 ISIQHH197 pKa = 4.0 YY198 pKa = 8.32 TGYY201 pKa = 10.97 FDD203 pKa = 3.53 MVLYY207 pKa = 8.48 KK208 pKa = 10.31 TLLATGCRR216 pKa = 11.84 IGEE219 pKa = 4.05 LLALNWSDD227 pKa = 3.85 IDD229 pKa = 4.27 FKK231 pKa = 11.51 EE232 pKa = 3.89 KK233 pKa = 9.84 TLNISKK239 pKa = 9.19 TYY241 pKa = 9.02 NGEE244 pKa = 3.65 MGLIGTTKK252 pKa = 10.33 SKK254 pKa = 10.91 AGNRR258 pKa = 11.84 TISVDD263 pKa = 3.53 TNTLNMLRR271 pKa = 11.84 LYY273 pKa = 10.34 NARR276 pKa = 11.84 QRR278 pKa = 11.84 QMFSEE283 pKa = 4.43 IGAPRR288 pKa = 11.84 PSVVFSAPTRR298 pKa = 11.84 LYY300 pKa = 10.41 QLRR303 pKa = 11.84 TTIQRR308 pKa = 11.84 RR309 pKa = 11.84 LDD311 pKa = 4.24 DD312 pKa = 3.92 YY313 pKa = 11.32 CHH315 pKa = 6.78 TIDD318 pKa = 3.84 IPRR321 pKa = 11.84 FTLHH325 pKa = 7.29 AFRR328 pKa = 11.84 HH329 pKa = 4.72 THH331 pKa = 7.12 ASLLLNAGISYY342 pKa = 10.68 KK343 pKa = 10.23 EE344 pKa = 3.61 LQYY347 pKa = 11.51 RR348 pKa = 11.84 LGHH351 pKa = 6.41 ANISMTLDD359 pKa = 3.58 VYY361 pKa = 11.5 SHH363 pKa = 7.06 LSKK366 pKa = 11.04 DD367 pKa = 3.52 KK368 pKa = 10.43 EE369 pKa = 4.37 KK370 pKa = 11.05 EE371 pKa = 3.74 AVTYY375 pKa = 8.83 YY376 pKa = 10.86 EE377 pKa = 4.2 KK378 pKa = 10.84 AINNLL383 pKa = 3.4
Molecular weight: 44.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.136
IPC2_protein 9.297
IPC_protein 9.224
Toseland 9.867
ProMoST 9.589
Dawson 10.116
Bjellqvist 9.78
Wikipedia 10.277
Rodwell 10.526
Grimsley 10.189
Solomon 10.131
Lehninger 10.087
Nozaki 9.867
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.292
Sillero 10.014
Patrickios 9.443
IPC_peptide 10.116
IPC2_peptide 8.317
IPC2.peptide.svr19 8.267
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22
0
22
3805
59
474
173.0
19.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.938 ± 0.416
0.42 ± 0.139
5.834 ± 0.368
7.963 ± 0.698
4.074 ± 0.382
4.625 ± 0.471
2.208 ± 0.242
7.201 ± 0.324
8.673 ± 0.407
10.145 ± 0.567
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.024 ± 0.263
4.967 ± 0.361
3.259 ± 0.337
4.625 ± 0.272
5.151 ± 0.384
5.02 ± 0.316
5.887 ± 0.515
5.466 ± 0.334
0.841 ± 0.156
4.678 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here