uncultured phage_MedDCM-OCT-S45-C4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Ashivirus; Ashivirus S45C4

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4P7M7|A0A6S4P7M7_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S45-C4 OX=2740801 PE=4 SV=1
MM1 pKa = 7.57TDD3 pKa = 4.59LYY5 pKa = 11.47NALCDD10 pKa = 4.04RR11 pKa = 11.84FDD13 pKa = 5.61DD14 pKa = 3.64MDD16 pKa = 4.46EE17 pKa = 4.34IKK19 pKa = 10.81DD20 pKa = 3.65VAEE23 pKa = 4.22YY24 pKa = 10.79GCAAGVSGFIYY35 pKa = 9.48STEE38 pKa = 3.74LAEE41 pKa = 5.62FFDD44 pKa = 3.63KK45 pKa = 11.14HH46 pKa = 5.79EE47 pKa = 4.27EE48 pKa = 4.14EE49 pKa = 5.87IEE51 pKa = 4.45DD52 pKa = 3.72EE53 pKa = 4.6LDD55 pKa = 3.3ALGYY59 pKa = 10.27RR60 pKa = 11.84FQDD63 pKa = 4.01LVDD66 pKa = 3.41TDD68 pKa = 3.81EE69 pKa = 5.19FYY71 pKa = 11.14TMQEE75 pKa = 3.96IKK77 pKa = 10.53EE78 pKa = 4.12KK79 pKa = 9.81AVWVIVEE86 pKa = 4.34TFCHH90 pKa = 5.74NKK92 pKa = 9.21VDD94 pKa = 3.86AAYY97 pKa = 10.14DD98 pKa = 3.5AAA100 pKa = 6.11

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4P9K2|A0A6S4P9K2_9CAUD T7-like capsid assembly protein OS=uncultured phage_MedDCM-OCT-S45-C4 OX=2740801 PE=4 SV=1
MM1 pKa = 7.82TYY3 pKa = 10.1RR4 pKa = 11.84PHH6 pKa = 7.41RR7 pKa = 11.84RR8 pKa = 11.84TLTSTIKK15 pKa = 10.51VGKK18 pKa = 8.79HH19 pKa = 3.96LCKK22 pKa = 10.72VFIKK26 pKa = 10.3PWDD29 pKa = 3.59KK30 pKa = 10.44MPNGAVVWKK39 pKa = 10.57VGFGVGKK46 pKa = 9.62SRR48 pKa = 11.84RR49 pKa = 11.84QINDD53 pKa = 2.93WYY55 pKa = 10.62HH56 pKa = 5.43VKK58 pKa = 10.27QNRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84SLHH67 pKa = 4.65KK68 pKa = 10.36HH69 pKa = 3.44MTGTEE74 pKa = 4.16GFKK77 pKa = 10.33TIPRR81 pKa = 11.84GFNEE85 pKa = 3.85VLRR88 pKa = 11.84LRR90 pKa = 11.84WLIPAGDD97 pKa = 4.38TIFIDD102 pKa = 4.11CTSADD107 pKa = 4.36PEE109 pKa = 4.5KK110 pKa = 10.45QWKK113 pKa = 7.45TFSRR117 pKa = 11.84WRR119 pKa = 11.84RR120 pKa = 11.84WHH122 pKa = 6.71PDD124 pKa = 2.24WFVNEE129 pKa = 4.25HH130 pKa = 6.03LKK132 pKa = 10.37EE133 pKa = 4.5FYY135 pKa = 7.55WTRR138 pKa = 11.84PP139 pKa = 3.32

Molecular weight: 16.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12982

33

1326

244.9

27.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.005 ± 0.348

0.778 ± 0.193

6.625 ± 0.333

5.723 ± 0.432

3.667 ± 0.198

7.664 ± 0.448

1.641 ± 0.283

5.33 ± 0.28

5.4 ± 0.38

7.834 ± 0.433

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.207

5.115 ± 0.352

4.006 ± 0.239

4.398 ± 0.379

4.876 ± 0.336

6.871 ± 0.342

7.433 ± 0.655

6.178 ± 0.231

1.279 ± 0.143

3.628 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski