Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S45-C4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.015AlaAla: 10.015 ± 0.855
0.693AlaCys: 0.693 ± 0.276
5.701AlaAsp: 5.701 ± 0.786
6.702AlaGlu: 6.702 ± 1.102
4.083AlaPhe: 4.083 ± 0.461
7.935AlaGly: 7.935 ± 0.858
0.77AlaHis: 0.77 ± 0.261
4.622AlaIle: 4.622 ± 0.626
4.083AlaLys: 4.083 ± 0.827
7.087AlaLeu: 7.087 ± 0.78
2.157AlaMet: 2.157 ± 0.359
3.929AlaAsn: 3.929 ± 0.531
3.313AlaPro: 3.313 ± 0.505
4.853AlaGln: 4.853 ± 0.691
3.929AlaArg: 3.929 ± 0.721
5.624AlaSer: 5.624 ± 0.553
6.086AlaThr: 6.086 ± 1.284
5.084AlaVal: 5.084 ± 0.466
0.77AlaTrp: 0.77 ± 0.212
2.619AlaTyr: 2.619 ± 0.394
0.0AlaXaa: 0.0 ± 0.0
Cys
0.539CysAla: 0.539 ± 0.249
0.077CysCys: 0.077 ± 0.077
0.385CysAsp: 0.385 ± 0.167
0.539CysGlu: 0.539 ± 0.259
0.231CysPhe: 0.231 ± 0.119
0.462CysGly: 0.462 ± 0.248
0.154CysHis: 0.154 ± 0.112
0.462CysIle: 0.462 ± 0.191
0.924CysLys: 0.924 ± 0.329
0.462CysLeu: 0.462 ± 0.247
0.308CysMet: 0.308 ± 0.167
0.308CysAsn: 0.308 ± 0.161
0.154CysPro: 0.154 ± 0.106
0.462CysGln: 0.462 ± 0.179
0.077CysArg: 0.077 ± 0.077
0.616CysSer: 0.616 ± 0.265
0.924CysThr: 0.924 ± 0.296
0.308CysVal: 0.308 ± 0.172
0.154CysTrp: 0.154 ± 0.121
0.231CysTyr: 0.231 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
6.24AspAla: 6.24 ± 0.648
0.539AspCys: 0.539 ± 0.207
4.699AspAsp: 4.699 ± 0.891
4.314AspGlu: 4.314 ± 0.677
1.926AspPhe: 1.926 ± 0.344
5.007AspGly: 5.007 ± 0.6
0.924AspHis: 0.924 ± 0.351
3.775AspIle: 3.775 ± 0.454
3.158AspLys: 3.158 ± 0.483
5.855AspLeu: 5.855 ± 0.608
1.926AspMet: 1.926 ± 0.457
2.927AspAsn: 2.927 ± 0.514
3.852AspPro: 3.852 ± 0.755
2.157AspGln: 2.157 ± 0.39
2.542AspArg: 2.542 ± 0.551
3.544AspSer: 3.544 ± 0.518
4.006AspThr: 4.006 ± 0.619
4.776AspVal: 4.776 ± 0.576
1.233AspTrp: 1.233 ± 0.375
2.85AspTyr: 2.85 ± 0.445
0.0AspXaa: 0.0 ± 0.0
Glu
6.086GluAla: 6.086 ± 1.018
0.462GluCys: 0.462 ± 0.191
2.234GluAsp: 2.234 ± 0.373
4.16GluGlu: 4.16 ± 0.685
2.542GluPhe: 2.542 ± 0.422
3.929GluGly: 3.929 ± 0.522
1.001GluHis: 1.001 ± 0.341
3.158GluIle: 3.158 ± 0.506
2.388GluLys: 2.388 ± 0.429
7.164GluLeu: 7.164 ± 0.758
1.387GluMet: 1.387 ± 0.329
2.157GluAsn: 2.157 ± 0.278
1.464GluPro: 1.464 ± 0.395
2.85GluGln: 2.85 ± 0.581
2.234GluArg: 2.234 ± 0.586
3.158GluSer: 3.158 ± 0.624
3.313GluThr: 3.313 ± 0.473
4.083GluVal: 4.083 ± 0.431
1.156GluTrp: 1.156 ± 0.332
2.311GluTyr: 2.311 ± 0.391
0.0GluXaa: 0.0 ± 0.0
Phe
2.08PheAla: 2.08 ± 0.356
0.539PheCys: 0.539 ± 0.226
3.158PheAsp: 3.158 ± 0.555
1.541PheGlu: 1.541 ± 0.412
1.772PhePhe: 1.772 ± 0.318
2.542PheGly: 2.542 ± 0.429
0.77PheHis: 0.77 ± 0.276
2.542PheIle: 2.542 ± 0.403
2.157PheLys: 2.157 ± 0.288
2.619PheLeu: 2.619 ± 0.582
1.078PheMet: 1.078 ± 0.312
2.619PheAsn: 2.619 ± 0.51
1.001PhePro: 1.001 ± 0.31
1.618PheGln: 1.618 ± 0.432
1.926PheArg: 1.926 ± 0.316
1.849PheSer: 1.849 ± 0.459
2.773PheThr: 2.773 ± 0.592
2.157PheVal: 2.157 ± 0.24
0.385PheTrp: 0.385 ± 0.173
1.464PheTyr: 1.464 ± 0.376
0.0PheXaa: 0.0 ± 0.0
Gly
5.624GlyAla: 5.624 ± 0.617
0.539GlyCys: 0.539 ± 0.195
5.547GlyAsp: 5.547 ± 0.552
4.083GlyGlu: 4.083 ± 0.732
2.542GlyPhe: 2.542 ± 0.548
6.625GlyGly: 6.625 ± 1.722
1.156GlyHis: 1.156 ± 0.296
3.621GlyIle: 3.621 ± 0.674
5.007GlyLys: 5.007 ± 0.675
5.084GlyLeu: 5.084 ± 0.714
2.234GlyMet: 2.234 ± 0.461
4.16GlyAsn: 4.16 ± 0.698
2.311GlyPro: 2.311 ± 0.473
3.467GlyGln: 3.467 ± 0.502
3.081GlyArg: 3.081 ± 0.54
5.547GlySer: 5.547 ± 1.239
6.086GlyThr: 6.086 ± 0.906
5.778GlyVal: 5.778 ± 0.663
0.924GlyTrp: 0.924 ± 0.263
3.235GlyTyr: 3.235 ± 0.544
0.0GlyXaa: 0.0 ± 0.0
His
0.77HisAla: 0.77 ± 0.279
0.231HisCys: 0.231 ± 0.114
0.924HisAsp: 0.924 ± 0.288
1.001HisGlu: 1.001 ± 0.304
0.77HisPhe: 0.77 ± 0.294
1.001HisGly: 1.001 ± 0.338
0.462HisHis: 0.462 ± 0.239
0.539HisIle: 0.539 ± 0.205
1.233HisLys: 1.233 ± 0.324
1.387HisLeu: 1.387 ± 0.483
0.847HisMet: 0.847 ± 0.245
0.847HisAsn: 0.847 ± 0.288
1.156HisPro: 1.156 ± 0.257
0.77HisGln: 0.77 ± 0.312
1.31HisArg: 1.31 ± 0.398
0.462HisSer: 0.462 ± 0.165
0.924HisThr: 0.924 ± 0.306
0.847HisVal: 0.847 ± 0.233
0.077HisTrp: 0.077 ± 0.077
0.847HisTyr: 0.847 ± 0.298
0.0HisXaa: 0.0 ± 0.0
Ile
5.161IleAla: 5.161 ± 0.618
0.308IleCys: 0.308 ± 0.144
4.545IleAsp: 4.545 ± 0.615
2.542IleGlu: 2.542 ± 0.445
1.541IlePhe: 1.541 ± 0.351
3.467IleGly: 3.467 ± 0.605
0.77IleHis: 0.77 ± 0.313
2.465IleIle: 2.465 ± 0.409
3.621IleLys: 3.621 ± 0.491
4.006IleLeu: 4.006 ± 0.564
0.77IleMet: 0.77 ± 0.301
2.85IleAsn: 2.85 ± 0.457
2.619IlePro: 2.619 ± 0.411
2.234IleGln: 2.234 ± 0.408
2.157IleArg: 2.157 ± 0.338
4.237IleSer: 4.237 ± 0.648
4.545IleThr: 4.545 ± 1.102
3.081IleVal: 3.081 ± 0.471
0.77IleTrp: 0.77 ± 0.241
1.618IleTyr: 1.618 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
4.853LysAla: 4.853 ± 0.848
0.154LysCys: 0.154 ± 0.133
3.158LysAsp: 3.158 ± 0.521
2.85LysGlu: 2.85 ± 0.42
1.31LysPhe: 1.31 ± 0.359
2.619LysGly: 2.619 ± 0.67
1.156LysHis: 1.156 ± 0.351
3.467LysIle: 3.467 ± 0.439
3.081LysLys: 3.081 ± 0.606
5.624LysLeu: 5.624 ± 0.783
1.233LysMet: 1.233 ± 0.307
2.542LysAsn: 2.542 ± 0.445
2.542LysPro: 2.542 ± 0.569
1.926LysGln: 1.926 ± 0.336
3.698LysArg: 3.698 ± 0.664
3.313LysSer: 3.313 ± 0.5
4.545LysThr: 4.545 ± 0.454
2.542LysVal: 2.542 ± 0.385
0.77LysTrp: 0.77 ± 0.211
2.619LysTyr: 2.619 ± 0.43
0.0LysXaa: 0.0 ± 0.0
Leu
6.548LeuAla: 6.548 ± 0.757
0.616LeuCys: 0.616 ± 0.279
4.776LeuAsp: 4.776 ± 0.653
4.237LeuGlu: 4.237 ± 0.568
2.696LeuPhe: 2.696 ± 0.484
5.932LeuGly: 5.932 ± 0.7
1.695LeuHis: 1.695 ± 0.405
3.467LeuIle: 3.467 ± 0.539
4.699LeuLys: 4.699 ± 0.709
5.161LeuLeu: 5.161 ± 0.771
1.849LeuMet: 1.849 ± 0.392
4.237LeuAsn: 4.237 ± 0.554
4.16LeuPro: 4.16 ± 0.592
3.698LeuGln: 3.698 ± 0.758
5.161LeuArg: 5.161 ± 0.751
6.548LeuSer: 6.548 ± 0.619
5.238LeuThr: 5.238 ± 0.675
4.699LeuVal: 4.699 ± 0.815
0.385LeuTrp: 0.385 ± 0.173
2.542LeuTyr: 2.542 ± 0.492
0.0LeuXaa: 0.0 ± 0.0
Met
2.85MetAla: 2.85 ± 0.463
0.154MetCys: 0.154 ± 0.098
1.464MetAsp: 1.464 ± 0.383
1.156MetGlu: 1.156 ± 0.307
1.156MetPhe: 1.156 ± 0.252
1.695MetGly: 1.695 ± 0.394
0.616MetHis: 0.616 ± 0.302
1.156MetIle: 1.156 ± 0.357
1.849MetLys: 1.849 ± 0.499
2.08MetLeu: 2.08 ± 0.372
0.385MetMet: 0.385 ± 0.212
1.078MetAsn: 1.078 ± 0.304
1.31MetPro: 1.31 ± 0.295
1.387MetGln: 1.387 ± 0.308
1.464MetArg: 1.464 ± 0.341
1.541MetSer: 1.541 ± 0.338
1.849MetThr: 1.849 ± 0.349
1.387MetVal: 1.387 ± 0.279
0.231MetTrp: 0.231 ± 0.134
0.693MetTyr: 0.693 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
4.006AsnAla: 4.006 ± 0.626
0.154AsnCys: 0.154 ± 0.098
3.39AsnAsp: 3.39 ± 0.532
2.619AsnGlu: 2.619 ± 0.435
2.465AsnPhe: 2.465 ± 0.431
4.853AsnGly: 4.853 ± 0.705
0.77AsnHis: 0.77 ± 0.264
2.234AsnIle: 2.234 ± 0.539
1.695AsnLys: 1.695 ± 0.337
4.314AsnLeu: 4.314 ± 0.474
1.078AsnMet: 1.078 ± 0.224
2.927AsnAsn: 2.927 ± 0.511
2.542AsnPro: 2.542 ± 0.359
1.695AsnGln: 1.695 ± 0.428
2.696AsnArg: 2.696 ± 0.47
3.929AsnSer: 3.929 ± 0.873
3.39AsnThr: 3.39 ± 0.477
3.621AsnVal: 3.621 ± 0.537
0.616AsnTrp: 0.616 ± 0.197
2.157AsnTyr: 2.157 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
2.927ProAla: 2.927 ± 0.469
0.154ProCys: 0.154 ± 0.108
3.081ProAsp: 3.081 ± 0.411
3.235ProGlu: 3.235 ± 0.794
1.464ProPhe: 1.464 ± 0.291
2.696ProGly: 2.696 ± 0.563
0.539ProHis: 0.539 ± 0.218
2.388ProIle: 2.388 ± 0.542
2.465ProLys: 2.465 ± 0.556
2.003ProLeu: 2.003 ± 0.446
1.001ProMet: 1.001 ± 0.359
2.157ProAsn: 2.157 ± 0.354
1.618ProPro: 1.618 ± 0.519
1.849ProGln: 1.849 ± 0.435
1.464ProArg: 1.464 ± 0.315
2.619ProSer: 2.619 ± 0.384
3.158ProThr: 3.158 ± 0.501
2.773ProVal: 2.773 ± 0.456
1.078ProTrp: 1.078 ± 0.242
1.387ProTyr: 1.387 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
5.161GlnAla: 5.161 ± 0.877
0.385GlnCys: 0.385 ± 0.15
2.465GlnAsp: 2.465 ± 0.484
2.311GlnGlu: 2.311 ± 0.456
1.926GlnPhe: 1.926 ± 0.334
2.542GlnGly: 2.542 ± 0.401
0.924GlnHis: 0.924 ± 0.277
2.311GlnIle: 2.311 ± 0.464
2.696GlnLys: 2.696 ± 0.594
3.852GlnLeu: 3.852 ± 0.636
1.156GlnMet: 1.156 ± 0.379
1.849GlnAsn: 1.849 ± 0.439
1.233GlnPro: 1.233 ± 0.343
2.696GlnGln: 2.696 ± 0.652
2.696GlnArg: 2.696 ± 0.485
2.157GlnSer: 2.157 ± 0.355
3.004GlnThr: 3.004 ± 0.456
3.158GlnVal: 3.158 ± 0.575
0.231GlnTrp: 0.231 ± 0.127
1.233GlnTyr: 1.233 ± 0.327
0.0GlnXaa: 0.0 ± 0.0
Arg
4.468ArgAla: 4.468 ± 0.787
0.539ArgCys: 0.539 ± 0.226
3.081ArgAsp: 3.081 ± 0.401
1.772ArgGlu: 1.772 ± 0.403
1.387ArgPhe: 1.387 ± 0.294
3.004ArgGly: 3.004 ± 0.533
0.693ArgHis: 0.693 ± 0.263
3.313ArgIle: 3.313 ± 0.556
2.85ArgLys: 2.85 ± 0.777
4.237ArgLeu: 4.237 ± 0.554
1.31ArgMet: 1.31 ± 0.34
2.234ArgAsn: 2.234 ± 0.325
1.849ArgPro: 1.849 ± 0.326
1.926ArgGln: 1.926 ± 0.393
3.158ArgArg: 3.158 ± 0.741
4.16ArgSer: 4.16 ± 0.521
3.621ArgThr: 3.621 ± 0.534
2.542ArgVal: 2.542 ± 0.4
0.847ArgTrp: 0.847 ± 0.302
1.772ArgTyr: 1.772 ± 0.341
0.0ArgXaa: 0.0 ± 0.0
Ser
6.856SerAla: 6.856 ± 0.908
0.539SerCys: 0.539 ± 0.174
4.699SerAsp: 4.699 ± 0.582
3.621SerGlu: 3.621 ± 0.585
3.004SerPhe: 3.004 ± 0.604
7.01SerGly: 7.01 ± 1.159
1.001SerHis: 1.001 ± 0.281
3.698SerIle: 3.698 ± 0.497
2.542SerLys: 2.542 ± 0.436
4.776SerLeu: 4.776 ± 0.742
1.926SerMet: 1.926 ± 0.395
3.621SerAsn: 3.621 ± 0.629
2.388SerPro: 2.388 ± 0.381
2.542SerGln: 2.542 ± 0.503
3.235SerArg: 3.235 ± 0.552
5.624SerSer: 5.624 ± 0.992
5.238SerThr: 5.238 ± 0.743
3.467SerVal: 3.467 ± 0.566
0.77SerTrp: 0.77 ± 0.282
2.157SerTyr: 2.157 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
6.317ThrAla: 6.317 ± 0.843
0.462ThrCys: 0.462 ± 0.232
4.468ThrAsp: 4.468 ± 0.661
4.16ThrGlu: 4.16 ± 0.58
2.234ThrPhe: 2.234 ± 0.452
6.548ThrGly: 6.548 ± 1.308
1.156ThrHis: 1.156 ± 0.306
4.853ThrIle: 4.853 ± 1.007
3.544ThrLys: 3.544 ± 0.485
5.238ThrLeu: 5.238 ± 0.562
1.464ThrMet: 1.464 ± 0.331
4.16ThrAsn: 4.16 ± 0.695
3.544ThrPro: 3.544 ± 0.384
2.234ThrGln: 2.234 ± 0.458
2.696ThrArg: 2.696 ± 0.49
6.163ThrSer: 6.163 ± 0.66
6.933ThrThr: 6.933 ± 1.146
4.314ThrVal: 4.314 ± 0.556
1.233ThrTrp: 1.233 ± 0.315
2.619ThrTyr: 2.619 ± 0.451
0.0ThrXaa: 0.0 ± 0.0
Val
5.778ValAla: 5.778 ± 0.532
0.693ValCys: 0.693 ± 0.241
4.468ValAsp: 4.468 ± 0.537
3.698ValGlu: 3.698 ± 0.497
2.08ValPhe: 2.08 ± 0.387
5.624ValGly: 5.624 ± 0.516
1.156ValHis: 1.156 ± 0.326
3.158ValIle: 3.158 ± 0.53
2.696ValLys: 2.696 ± 0.38
3.235ValLeu: 3.235 ± 0.469
1.541ValMet: 1.541 ± 0.28
3.235ValAsn: 3.235 ± 0.42
1.772ValPro: 1.772 ± 0.369
3.081ValGln: 3.081 ± 0.473
2.773ValArg: 2.773 ± 0.415
4.622ValSer: 4.622 ± 0.477
5.315ValThr: 5.315 ± 0.795
3.698ValVal: 3.698 ± 0.509
0.77ValTrp: 0.77 ± 0.242
2.388ValTyr: 2.388 ± 0.545
0.0ValXaa: 0.0 ± 0.0
Trp
0.539TrpAla: 0.539 ± 0.179
0.154TrpCys: 0.154 ± 0.101
0.77TrpAsp: 0.77 ± 0.261
1.001TrpGlu: 1.001 ± 0.288
0.693TrpPhe: 0.693 ± 0.241
0.077TrpGly: 0.077 ± 0.078
0.308TrpHis: 0.308 ± 0.148
0.462TrpIle: 0.462 ± 0.155
0.847TrpLys: 0.847 ± 0.308
1.695TrpLeu: 1.695 ± 0.36
0.77TrpMet: 0.77 ± 0.244
0.924TrpAsn: 0.924 ± 0.266
0.385TrpPro: 0.385 ± 0.131
0.231TrpGln: 0.231 ± 0.127
0.308TrpArg: 0.308 ± 0.154
1.156TrpSer: 1.156 ± 0.288
0.924TrpThr: 0.924 ± 0.27
1.078TrpVal: 1.078 ± 0.238
0.077TrpTrp: 0.077 ± 0.072
0.385TrpTyr: 0.385 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.235TyrAla: 3.235 ± 0.465
0.385TyrCys: 0.385 ± 0.193
3.235TyrAsp: 3.235 ± 0.484
1.695TyrGlu: 1.695 ± 0.345
0.847TyrPhe: 0.847 ± 0.197
3.081TyrGly: 3.081 ± 0.563
0.385TyrHis: 0.385 ± 0.175
1.618TyrIle: 1.618 ± 0.393
2.465TyrLys: 2.465 ± 0.498
2.465TyrLeu: 2.465 ± 0.411
1.001TyrMet: 1.001 ± 0.275
2.388TyrAsn: 2.388 ± 0.451
0.847TyrPro: 0.847 ± 0.238
2.311TyrGln: 2.311 ± 0.439
2.157TyrArg: 2.157 ± 0.469
1.849TyrSer: 1.849 ± 0.32
2.465TyrThr: 2.465 ± 0.411
2.388TyrVal: 2.388 ± 0.445
0.308TyrTrp: 0.308 ± 0.173
1.156TyrTyr: 1.156 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12982 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski