Microcystis virus Ma-LMM01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Fukuivirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7J5|A0A7J5_9CAUD Uncharacterized protein OS=Microcystis virus Ma-LMM01 OX=340435 PE=4 SV=1
MM1 pKa = 8.1IYY3 pKa = 10.33ISITFSQHH11 pKa = 6.33QSLDD15 pKa = 3.23WSDD18 pKa = 3.09WSHH21 pKa = 5.9TVGEE25 pKa = 4.85DD26 pKa = 3.31AFGWLQTAGEE36 pKa = 4.28DD37 pKa = 3.67NIIVEE42 pKa = 4.19KK43 pKa = 10.83LEE45 pKa = 4.27PYY47 pKa = 10.0EE48 pKa = 4.05AVLAIEE54 pKa = 5.82DD55 pKa = 5.22DD56 pKa = 3.45IADD59 pKa = 4.19NIGYY63 pKa = 8.49ICAMLIEE70 pKa = 4.72HH71 pKa = 6.39GVVEE75 pKa = 4.98FNVHH79 pKa = 5.92VSPTQLAWW87 pKa = 3.55

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H1|A0A7H1_9CAUD Uncharacterized protein OS=Microcystis virus Ma-LMM01 OX=340435 PE=4 SV=1
MM1 pKa = 7.5SNYY4 pKa = 9.94PIYY7 pKa = 10.85LNSLEE12 pKa = 4.42IIPRR16 pKa = 11.84HH17 pKa = 5.77RR18 pKa = 11.84EE19 pKa = 3.4LVVRR23 pKa = 11.84VRR25 pKa = 11.84QARR28 pKa = 11.84PHH30 pKa = 5.86VKK32 pKa = 9.78KK33 pKa = 10.68APAGGTSHH41 pKa = 6.35QVWPYY46 pKa = 8.97HH47 pKa = 4.53GARR50 pKa = 11.84RR51 pKa = 11.84IEE53 pKa = 4.2EE54 pKa = 4.25WPSTT58 pKa = 3.78

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

184

0

184

50219

40

3562

272.9

30.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.686 ± 0.229

0.956 ± 0.103

5.807 ± 0.173

5.536 ± 0.135

3.071 ± 0.108

6.818 ± 0.186

1.663 ± 0.112

6.93 ± 0.15

3.771 ± 0.197

9.863 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.131

5.213 ± 0.14

4.761 ± 0.168

3.449 ± 0.11

6.267 ± 0.202

7.087 ± 0.175

6.587 ± 0.201

6.464 ± 0.148

1.38 ± 0.093

4.347 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski