Xanthomonas phage vB_XveM_DIBBI
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3PGT6|I3PGT6_9CAUD Terminase small subunit OS=Xanthomonas phage vB_XveM_DIBBI OX=1129194 GN=DIBBI_003 PE=4 SV=1
MM1 pKa = 8.31 DD2 pKa = 6.12 DD3 pKa = 4.1 FDD5 pKa = 6.17 LMHH8 pKa = 7.51 LDD10 pKa = 3.38 TEE12 pKa = 4.22 EE13 pKa = 4.73 AEE15 pKa = 4.2 RR16 pKa = 11.84 KK17 pKa = 8.87 AAEE20 pKa = 4.06 VADD23 pKa = 4.61 RR24 pKa = 11.84 KK25 pKa = 10.74 PEE27 pKa = 3.96 VEE29 pKa = 5.09 DD30 pKa = 5.8 DD31 pKa = 3.73 EE32 pKa = 5.59 GDD34 pKa = 4.27 CEE36 pKa = 4.46 GCKK39 pKa = 9.58 II40 pKa = 4.48
Molecular weight: 4.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.285
IPC2_protein 3.999
IPC_protein 3.91
Toseland 3.719
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.024
Patrickios 3.694
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|I3PGY9|I3PGY9_9CAUD Putative membrane protein OS=Xanthomonas phage vB_XveM_DIBBI OX=1129194 GN=DIBBI_056 PE=4 SV=1
MM1 pKa = 7.47 SKK3 pKa = 11.07 ASIAVSNQLAATAVPVITEE22 pKa = 4.57 GGRR25 pKa = 11.84 QTWRR29 pKa = 11.84 KK30 pKa = 8.13 KK31 pKa = 10.79 LVMIPMLHH39 pKa = 6.72 HH40 pKa = 5.99 MAQYY44 pKa = 11.08 AEE46 pKa = 4.68 LMDD49 pKa = 3.8 KK50 pKa = 9.83 FHH52 pKa = 7.52 RR53 pKa = 11.84 LGSVPSHH60 pKa = 6.39 YY61 pKa = 9.98 YY62 pKa = 9.28 RR63 pKa = 11.84 HH64 pKa = 6.29 RR65 pKa = 11.84 LVITGEE71 pKa = 4.04 RR72 pKa = 11.84 LVEE75 pKa = 4.17 GAGEE79 pKa = 4.22 HH80 pKa = 6.8 GDD82 pKa = 3.76 PKK84 pKa = 11.17 VMPVYY89 pKa = 10.31 RR90 pKa = 11.84 FEE92 pKa = 4.02 HH93 pKa = 6.42 RR94 pKa = 11.84 KK95 pKa = 7.8 MKK97 pKa = 10.48 SRR99 pKa = 11.84 AKK101 pKa = 10.28 LDD103 pKa = 3.3 RR104 pKa = 11.84 CFPRR108 pKa = 11.84 GKK110 pKa = 10.11 AYY112 pKa = 10.38 RR113 pKa = 11.84 RR114 pKa = 11.84 IMDD117 pKa = 4.52 LARR120 pKa = 11.84 IRR122 pKa = 11.84 AAQVKK127 pKa = 10.17 AGVVV131 pKa = 3.23
Molecular weight: 14.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.882
IPC_protein 10.73
Toseland 10.862
ProMoST 10.584
Dawson 10.95
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 11.199
Grimsley 10.994
Solomon 11.082
Lehninger 11.052
Nozaki 10.847
DTASelect 10.672
Thurlkill 10.862
EMBOSS 11.272
Sillero 10.877
Patrickios 10.921
IPC_peptide 11.096
IPC2_peptide 9.575
IPC2.peptide.svr19 8.477
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
81
0
81
15012
24
852
185.3
20.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.512 ± 0.375
0.926 ± 0.116
6.295 ± 0.216
5.995 ± 0.337
3.69 ± 0.2
7.947 ± 0.353
1.972 ± 0.179
6.322 ± 0.211
5.755 ± 0.256
7.248 ± 0.207
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.858 ± 0.144
4.723 ± 0.193
3.491 ± 0.141
4.05 ± 0.162
5.642 ± 0.24
6.188 ± 0.243
5.842 ± 0.233
6.881 ± 0.221
1.359 ± 0.118
3.304 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here