Avian infectious bursal disease virus (isolate Chicken/UK/UK661/1989) (IBDV) (Gumboro disease virus)
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q82635|POLS_IBDVU Structural polyprotein OS=Avian infectious bursal disease virus (isolate Chicken/UK/UK661/1989) OX=644440 PE=3 SV=1
MM1 pKa = 7.71 TNLQDD6 pKa = 3.34 QTQQIVPFIRR16 pKa = 11.84 SLLMPTTGPASIPDD30 pKa = 3.64 DD31 pKa = 3.68 TLEE34 pKa = 3.92 KK35 pKa = 9.68 HH36 pKa = 5.47 TLRR39 pKa = 11.84 SEE41 pKa = 3.92 TSTYY45 pKa = 10.97 NLTVGDD51 pKa = 4.14 TGSGLIVFFPGFPGSIVGAHH71 pKa = 5.56 YY72 pKa = 9.29 TLQSNGNYY80 pKa = 10.13 KK81 pKa = 9.79 FDD83 pKa = 4.0 QMLLTAQNLPASYY96 pKa = 10.21 NYY98 pKa = 9.44 CRR100 pKa = 11.84 LVSRR104 pKa = 11.84 SLTVRR109 pKa = 11.84 SSTLPGGVYY118 pKa = 10.67 ALNGTINAVTFQGSLSEE135 pKa = 4.27 LTDD138 pKa = 3.21 VSYY141 pKa = 11.52 NGLMSATANINDD153 pKa = 4.22 KK154 pKa = 10.59 IGNVLVGEE162 pKa = 4.48 GVTVLSLPTSYY173 pKa = 11.5 DD174 pKa = 2.89 LGYY177 pKa = 10.9 VRR179 pKa = 11.84 LGDD182 pKa = 4.85 PIPAIGLDD190 pKa = 3.48 PKK192 pKa = 10.43 MVATCDD198 pKa = 3.2 SSDD201 pKa = 3.38 RR202 pKa = 11.84 PRR204 pKa = 11.84 VYY206 pKa = 10.49 TITAADD212 pKa = 4.51 DD213 pKa = 3.84 YY214 pKa = 11.05 QFSSQYY220 pKa = 9.39 QAGGVTITLFSANIDD235 pKa = 4.18 AITSLSIGGEE245 pKa = 4.04 LVFQTSVQGLILGATIYY262 pKa = 11.01 LIGFDD267 pKa = 3.55 GTAVITRR274 pKa = 11.84 AVAADD279 pKa = 3.49 NGLTAGTDD287 pKa = 2.99 NLMPFNIVIPTSEE300 pKa = 3.82 ITQPITSIKK309 pKa = 10.6 LEE311 pKa = 4.0 IVTSKK316 pKa = 10.91 SGGQAGDD323 pKa = 3.42 QMSWSASGSLAVTIHH338 pKa = 6.06 GGNYY342 pKa = 9.04 PGALRR347 pKa = 11.84 PVTLVAYY354 pKa = 9.1 EE355 pKa = 4.18 RR356 pKa = 11.84 VATGSVVTVAGVSNFEE372 pKa = 5.03 LIPNPEE378 pKa = 3.96 LAKK381 pKa = 11.03 NLVTEE386 pKa = 4.26 YY387 pKa = 11.44 GRR389 pKa = 11.84 FDD391 pKa = 4.12 PGAMNYY397 pKa = 7.66 TKK399 pKa = 10.75 LILSEE404 pKa = 4.11 RR405 pKa = 11.84 DD406 pKa = 2.95 RR407 pKa = 11.84 LGIKK411 pKa = 7.3 TVWPTRR417 pKa = 11.84 EE418 pKa = 3.79 YY419 pKa = 10.7 TDD421 pKa = 3.6 FRR423 pKa = 11.84 EE424 pKa = 4.25 YY425 pKa = 10.92 FMEE428 pKa = 4.29 VADD431 pKa = 4.92 LNSPLKK437 pKa = 10.41 IAGAFGFKK445 pKa = 10.41 DD446 pKa = 3.56 IIRR449 pKa = 11.84 ALRR452 pKa = 11.84 RR453 pKa = 11.84 IAVPVVSTLFPPAAPLAHH471 pKa = 7.26 AIGEE475 pKa = 4.5 GVDD478 pKa = 3.58 YY479 pKa = 11.29 LLGDD483 pKa = 3.95 EE484 pKa = 4.72 AQAASGTARR493 pKa = 11.84 AASGKK498 pKa = 9.96 ARR500 pKa = 11.84 AASGRR505 pKa = 11.84 IRR507 pKa = 11.84 QLTLAADD514 pKa = 3.27 KK515 pKa = 10.88 GYY517 pKa = 10.91 EE518 pKa = 4.07 VVANLFQVPQNPVVDD533 pKa = 5.83 GILASPGILRR543 pKa = 11.84 GAHH546 pKa = 6.41 NLDD549 pKa = 3.45 CVLRR553 pKa = 11.84 EE554 pKa = 4.02 GATLFPVVITTVEE567 pKa = 3.82 DD568 pKa = 3.49 AMTPKK573 pKa = 10.78 ALNSKK578 pKa = 8.69 MFAVIEE584 pKa = 4.28 GVRR587 pKa = 11.84 EE588 pKa = 3.92 DD589 pKa = 4.45 LQPPSQRR596 pKa = 11.84 GSFIRR601 pKa = 11.84 TLSGHH606 pKa = 5.86 RR607 pKa = 11.84 VYY609 pKa = 10.95 GYY611 pKa = 11.08 APDD614 pKa = 3.92 GVLPLEE620 pKa = 4.29 TGRR623 pKa = 11.84 VYY625 pKa = 10.6 TVVPIDD631 pKa = 4.05 GVWDD635 pKa = 4.07 DD636 pKa = 5.89 SIMLSKK642 pKa = 11.0 DD643 pKa = 3.59 PIPPIVGSSGNLAIAYY659 pKa = 8.15 MDD661 pKa = 3.36 VFRR664 pKa = 11.84 PKK666 pKa = 10.6 VPIHH670 pKa = 5.55 VAMTGALNAYY680 pKa = 9.78 GEE682 pKa = 4.32 IEE684 pKa = 4.15 NVSFRR689 pKa = 11.84 STKK692 pKa = 10.2 LATAHH697 pKa = 7.34 RR698 pKa = 11.84 LGLKK702 pKa = 10.02 LAGPGAFDD710 pKa = 4.78 VNTGSNWATFIKK722 pKa = 10.25 RR723 pKa = 11.84 FPHH726 pKa = 6.93 NPRR729 pKa = 11.84 DD730 pKa = 3.86 WDD732 pKa = 3.68 RR733 pKa = 11.84 LPYY736 pKa = 10.78 LNLPYY741 pKa = 10.53 LPPNAGRR748 pKa = 11.84 QYY750 pKa = 10.65 DD751 pKa = 3.84 LAMAASEE758 pKa = 4.11 FKK760 pKa = 9.23 EE761 pKa = 4.5 TPEE764 pKa = 4.24 LEE766 pKa = 4.34 SAVRR770 pKa = 11.84 AMEE773 pKa = 3.97 AAANVDD779 pKa = 3.86 PLFQSALSVFMWLEE793 pKa = 3.76 EE794 pKa = 4.06 NGIVTDD800 pKa = 3.69 MANFALSDD808 pKa = 4.05 PNAHH812 pKa = 7.07 RR813 pKa = 11.84 MRR815 pKa = 11.84 NFLANAPQAGSKK827 pKa = 7.76 SQRR830 pKa = 11.84 AKK832 pKa = 10.94 YY833 pKa = 8.02 GTAGYY838 pKa = 9.76 GVEE841 pKa = 4.38 ARR843 pKa = 11.84 GPTPEE848 pKa = 4.0 GAQRR852 pKa = 11.84 EE853 pKa = 4.16 KK854 pKa = 10.5 DD855 pKa = 3.36 TRR857 pKa = 11.84 ISKK860 pKa = 10.43 KK861 pKa = 9.06 MEE863 pKa = 3.68 TMGIYY868 pKa = 10.18 FATPEE873 pKa = 3.79 WVALNGHH880 pKa = 7.16 RR881 pKa = 11.84 GPSPGQLKK889 pKa = 9.33 YY890 pKa = 9.33 WQNTRR895 pKa = 11.84 EE896 pKa = 4.11 IPDD899 pKa = 3.93 PNEE902 pKa = 4.54 DD903 pKa = 3.6 YY904 pKa = 11.22 LDD906 pKa = 3.79 YY907 pKa = 11.55 VHH909 pKa = 7.37 AEE911 pKa = 3.96 KK912 pKa = 10.86 SRR914 pKa = 11.84 LASEE918 pKa = 4.28 GQILRR923 pKa = 11.84 AATSIYY929 pKa = 9.8 GAPGQAEE936 pKa = 4.26 PPQAFIDD943 pKa = 4.24 EE944 pKa = 4.4 VAKK947 pKa = 10.81 VYY949 pKa = 9.96 EE950 pKa = 4.51 VNHH953 pKa = 5.75 GRR955 pKa = 11.84 GPNQEE960 pKa = 3.86 QMKK963 pKa = 10.54 DD964 pKa = 3.44 LLLTAMEE971 pKa = 4.32 MKK973 pKa = 10.22 HH974 pKa = 5.99 RR975 pKa = 11.84 NPRR978 pKa = 11.84 RR979 pKa = 11.84 APPKK983 pKa = 9.75 PKK985 pKa = 9.82 PKK987 pKa = 10.16 PNVPTQRR994 pKa = 11.84 PPGRR998 pKa = 11.84 LGRR1001 pKa = 11.84 WIRR1004 pKa = 11.84 AVSDD1008 pKa = 3.5 EE1009 pKa = 4.29 DD1010 pKa = 4.85 LEE1012 pKa = 4.34
Molecular weight: 109.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.653
IPC2_protein 5.639
IPC_protein 5.664
Toseland 6.033
ProMoST 5.893
Dawson 5.855
Bjellqvist 5.906
Wikipedia 5.842
Rodwell 5.83
Grimsley 6.198
Solomon 5.855
Lehninger 5.842
Nozaki 6.084
DTASelect 6.275
Thurlkill 6.224
EMBOSS 6.198
Sillero 6.186
Patrickios 4.215
IPC_peptide 5.868
IPC2_peptide 6.173
IPC2.peptide.svr19 6.166
Protein with the highest isoelectric point:
>sp|Q82634|VP5_IBDVU Protein VP5 OS=Avian infectious bursal disease virus (isolate Chicken/UK/UK661/1989) OX=644440 GN=VP5 PE=3 SV=1
MM1 pKa = 7.68 SDD3 pKa = 3.42 VFNSPQARR11 pKa = 11.84 SKK13 pKa = 10.54 ISAAFGIKK21 pKa = 8.03 PTAGQDD27 pKa = 3.57 VEE29 pKa = 4.51 EE30 pKa = 4.15 LLIPKK35 pKa = 9.41 VWVPPEE41 pKa = 4.66 DD42 pKa = 3.98 PLASPSRR49 pKa = 11.84 LAKK52 pKa = 10.1 FLRR55 pKa = 11.84 EE56 pKa = 3.76 NGYY59 pKa = 10.57 KK60 pKa = 9.84 ILQPRR65 pKa = 11.84 SLPEE69 pKa = 3.8 NEE71 pKa = 4.84 EE72 pKa = 4.32 YY73 pKa = 9.31 EE74 pKa = 4.26 TDD76 pKa = 4.37 QILPDD81 pKa = 4.33 LAWMRR86 pKa = 11.84 QIEE89 pKa = 4.66 GPVLKK94 pKa = 8.86 PTLSLPIGDD103 pKa = 3.76 QEE105 pKa = 4.53 YY106 pKa = 10.05 FPKK109 pKa = 10.53 YY110 pKa = 10.12 YY111 pKa = 7.96 PTHH114 pKa = 6.68 RR115 pKa = 11.84 PSKK118 pKa = 8.84 EE119 pKa = 3.82 KK120 pKa = 10.69 PNAYY124 pKa = 9.47 PPDD127 pKa = 3.66 IALLKK132 pKa = 10.02 QMIYY136 pKa = 10.94 LFLQVPEE143 pKa = 4.26 ATDD146 pKa = 3.45 NLKK149 pKa = 11.12 DD150 pKa = 3.76 EE151 pKa = 4.63 VTLLTQNIRR160 pKa = 11.84 DD161 pKa = 3.64 KK162 pKa = 11.21 AYY164 pKa = 10.57 GSGTYY169 pKa = 9.06 MGQATRR175 pKa = 11.84 LVAMKK180 pKa = 10.14 EE181 pKa = 4.18 VATGRR186 pKa = 11.84 NPNKK190 pKa = 10.5 DD191 pKa = 3.73 PLKK194 pKa = 10.6 LGYY197 pKa = 8.52 TFEE200 pKa = 6.44 SIAQLLDD207 pKa = 2.76 ITLPVGPPGEE217 pKa = 4.58 DD218 pKa = 3.24 DD219 pKa = 4.36 KK220 pKa = 11.71 PWVPLTRR227 pKa = 11.84 VPSRR231 pKa = 11.84 MLVLTGDD238 pKa = 3.16 VDD240 pKa = 4.42 GEE242 pKa = 4.52 FEE244 pKa = 4.79 VEE246 pKa = 5.0 DD247 pKa = 4.15 YY248 pKa = 11.12 LPKK251 pKa = 10.38 INLKK255 pKa = 10.38 SSSGLPYY262 pKa = 10.07 VGRR265 pKa = 11.84 TKK267 pKa = 11.16 GEE269 pKa = 4.36 TIGEE273 pKa = 4.19 MIAISNQFLRR283 pKa = 11.84 EE284 pKa = 3.97 LSALLKK290 pKa = 10.64 QGAGTKK296 pKa = 10.27 GSNKK300 pKa = 9.63 KK301 pKa = 10.01 KK302 pKa = 9.94 LLSMLSDD309 pKa = 3.42 YY310 pKa = 10.62 WYY312 pKa = 10.51 LSCGLLFPKK321 pKa = 10.44 AEE323 pKa = 4.42 RR324 pKa = 11.84 YY325 pKa = 8.78 DD326 pKa = 4.02 KK327 pKa = 10.39 STCCTKK333 pKa = 10.12 TRR335 pKa = 11.84 NKK337 pKa = 9.43 WSAQSSTHH345 pKa = 6.99 LMISMITWPVMSNSPNNVLNIEE367 pKa = 4.55 GCPSLYY373 pKa = 10.44 KK374 pKa = 10.41 FNPFRR379 pKa = 11.84 GGLNRR384 pKa = 11.84 IVEE387 pKa = 4.89 WIMAPDD393 pKa = 3.58 EE394 pKa = 4.53 PKK396 pKa = 11.01 ALVYY400 pKa = 10.83 ADD402 pKa = 4.13 NIYY405 pKa = 10.24 IVHH408 pKa = 6.42 SNTWYY413 pKa = 10.94 SIDD416 pKa = 4.29 LEE418 pKa = 4.4 KK419 pKa = 11.59 GEE421 pKa = 4.61 ANCTRR426 pKa = 11.84 QHH428 pKa = 5.49 MQAAMYY434 pKa = 10.0 YY435 pKa = 8.69 ILTRR439 pKa = 11.84 GWSDD443 pKa = 3.09 NGDD446 pKa = 3.46 PMFNQTWATFAMNIAPALVVDD467 pKa = 4.6 SSCLIMNLQIKK478 pKa = 9.15 TYY480 pKa = 10.14 GQGSGNAATFINNHH494 pKa = 5.92 LLSTLVLDD502 pKa = 3.68 QWNLMKK508 pKa = 10.64 QPSPDD513 pKa = 3.39 SEE515 pKa = 4.18 EE516 pKa = 4.04 FKK518 pKa = 11.08 SIEE521 pKa = 4.4 DD522 pKa = 3.33 KK523 pKa = 11.34 LGINFKK529 pKa = 10.15 IEE531 pKa = 3.77 RR532 pKa = 11.84 SIDD535 pKa = 3.93 DD536 pKa = 2.97 IRR538 pKa = 11.84 GKK540 pKa = 10.36 LRR542 pKa = 11.84 QLVPLAQPGYY552 pKa = 10.0 LSGGVEE558 pKa = 4.36 PEE560 pKa = 4.12 QPSPTVEE567 pKa = 4.06 LDD569 pKa = 3.45 LLGWSATYY577 pKa = 10.83 SKK579 pKa = 11.21 DD580 pKa = 3.02 LGIYY584 pKa = 10.27 VPVLDD589 pKa = 5.2 KK590 pKa = 11.27 EE591 pKa = 4.59 RR592 pKa = 11.84 LFCSAAYY599 pKa = 9.04 PKK601 pKa = 10.69 GVEE604 pKa = 4.06 NKK606 pKa = 9.63 SLKK609 pKa = 10.27 SKK611 pKa = 10.93 VGIEE615 pKa = 3.75 QAYY618 pKa = 9.5 KK619 pKa = 8.92 VVRR622 pKa = 11.84 YY623 pKa = 7.56 EE624 pKa = 3.74 ALRR627 pKa = 11.84 LVGGWNYY634 pKa = 10.51 PLLNKK639 pKa = 9.72 ACKK642 pKa = 9.97 NNASAARR649 pKa = 11.84 RR650 pKa = 11.84 HH651 pKa = 5.75 LEE653 pKa = 3.98 AKK655 pKa = 10.1 GFPLDD660 pKa = 3.58 EE661 pKa = 5.47 FLAEE665 pKa = 3.87 WSEE668 pKa = 3.75 LSEE671 pKa = 4.25 FGEE674 pKa = 4.21 AFEE677 pKa = 4.89 GFNIKK682 pKa = 8.98 LTVTPEE688 pKa = 3.73 SLAEE692 pKa = 4.2 LNRR695 pKa = 11.84 PVPPKK700 pKa = 9.68 PPNVNRR706 pKa = 11.84 PVNTGGLKK714 pKa = 10.34 AVSNALKK721 pKa = 9.43 TGRR724 pKa = 11.84 YY725 pKa = 8.86 RR726 pKa = 11.84 NEE728 pKa = 3.92 AGLSGLVLLATARR741 pKa = 11.84 SRR743 pKa = 11.84 LQDD746 pKa = 3.25 AVKK749 pKa = 10.91 AMAEE753 pKa = 3.94 AEE755 pKa = 4.34 KK756 pKa = 10.6 LHH758 pKa = 6.51 KK759 pKa = 10.57 SKK761 pKa = 10.83 PDD763 pKa = 3.84 DD764 pKa = 4.69 PDD766 pKa = 5.73 ADD768 pKa = 3.12 WFEE771 pKa = 4.42 RR772 pKa = 11.84 SEE774 pKa = 4.24 TLSDD778 pKa = 3.95 LLEE781 pKa = 4.43 KK782 pKa = 10.72 ADD784 pKa = 3.58 IASKK788 pKa = 9.84 VAHH791 pKa = 6.17 SALVEE796 pKa = 4.02 TSDD799 pKa = 4.36 ALEE802 pKa = 4.48 AVQSTSVYY810 pKa = 7.57 TPKK813 pKa = 10.61 YY814 pKa = 9.41 PEE816 pKa = 3.92 VKK818 pKa = 10.27 NPQTASHH825 pKa = 6.48 PVVGLHH831 pKa = 6.69 LPAKK835 pKa = 10.0 RR836 pKa = 11.84 ATGVQAALLGAGTSRR851 pKa = 11.84 PMGMEE856 pKa = 3.61 APTRR860 pKa = 11.84 SKK862 pKa = 11.17 NAVKK866 pKa = 9.47 MAKK869 pKa = 9.48 RR870 pKa = 11.84 RR871 pKa = 11.84 QRR873 pKa = 11.84 QKK875 pKa = 10.89 EE876 pKa = 4.04 SRR878 pKa = 11.84 QQ879 pKa = 3.42
Molecular weight: 97.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.954
IPC2_protein 7.454
IPC_protein 7.337
Toseland 7.146
ProMoST 7.863
Dawson 8.097
Bjellqvist 8.287
Wikipedia 8.039
Rodwell 8.126
Grimsley 7.205
Solomon 8.17
Lehninger 8.17
Nozaki 8.404
DTASelect 8.126
Thurlkill 8.2
EMBOSS 8.302
Sillero 8.463
Patrickios 4.469
IPC_peptide 8.156
IPC2_peptide 7.132
IPC2.peptide.svr19 7.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2040
149
1012
680.0
74.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.775 ± 1.134
0.882 ± 0.91
5.196 ± 0.677
5.686 ± 0.653
2.892 ± 0.435
7.304 ± 1.104
1.667 ± 1.179
4.608 ± 1.143
5.049 ± 1.354
9.804 ± 0.676
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.24
4.755 ± 0.208
7.402 ± 0.18
3.676 ± 0.399
5.441 ± 0.967
7.206 ± 0.586
6.078 ± 0.645
6.373 ± 0.634
1.471 ± 0.652
3.382 ± 0.788
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here