Listeria floridensis FSL S10-1187
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2904 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W7CAD2|W7CAD2_9LIST Uncharacterized protein OS=Listeria floridensis FSL S10-1187 OX=1265817 GN=MFLO_01215 PE=4 SV=1
MM1 pKa = 7.86 IDD3 pKa = 3.19 GKK5 pKa = 7.93 TNRR8 pKa = 11.84 FDD10 pKa = 3.93 YY11 pKa = 10.7 SGCVYY16 pKa = 10.63 PIGLVPDD23 pKa = 3.4 EE24 pKa = 3.7 VLYY27 pKa = 11.0 FNEE30 pKa = 4.03 EE31 pKa = 4.15 NIDD34 pKa = 3.64 EE35 pKa = 4.26 VLFAGFYY42 pKa = 10.83 DD43 pKa = 4.12 DD44 pKa = 6.37 DD45 pKa = 3.9 EE46 pKa = 6.33 ARR48 pKa = 11.84 FQTLYY53 pKa = 11.25 SDD55 pKa = 3.17 WSEE58 pKa = 4.1 NNASGIEE65 pKa = 3.98 KK66 pKa = 10.56 GVVDD70 pKa = 5.13 KK71 pKa = 11.01 PLL73 pKa = 4.56
Molecular weight: 8.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|W7C958|W7C958_9LIST 30S ribosomal protein S2 OS=Listeria floridensis FSL S10-1187 OX=1265817 GN=rpsB PE=3 SV=1
MM1 pKa = 7.12 HH2 pKa = 7.55 RR3 pKa = 11.84 CLFFLLYY10 pKa = 10.07 FKK12 pKa = 10.55 VVKK15 pKa = 10.07 RR16 pKa = 11.84 GSIALFIVFLTSCGIEE32 pKa = 4.17 VVQLIQTTLVTGFSGMTLPSGRR54 pKa = 11.84 STDD57 pKa = 3.12 IDD59 pKa = 4.84 DD60 pKa = 5.44 IILNTLSGSLGVLIIYY76 pKa = 7.8 LIPNFRR82 pKa = 11.84 KK83 pKa = 10.11 RR84 pKa = 11.84 IGRR87 pKa = 11.84 KK88 pKa = 8.56 KK89 pKa = 10.31 RR90 pKa = 11.84 RR91 pKa = 3.46
Molecular weight: 10.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.78
IPC_protein 10.599
Toseland 10.847
ProMoST 10.965
Dawson 10.921
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.169
Grimsley 10.965
Solomon 11.067
Lehninger 11.038
Nozaki 10.847
DTASelect 10.643
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.862
Patrickios 10.95
IPC_peptide 11.082
IPC2_peptide 9.78
IPC2.peptide.svr19 8.68
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2904
0
2904
737768
22
2800
254.1
28.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.9 ± 0.055
0.583 ± 0.013
5.384 ± 0.045
7.376 ± 0.067
4.754 ± 0.054
6.897 ± 0.042
1.79 ± 0.024
7.271 ± 0.049
6.994 ± 0.044
9.747 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.608 ± 0.027
4.296 ± 0.04
3.421 ± 0.028
3.529 ± 0.03
4.241 ± 0.033
5.976 ± 0.033
5.784 ± 0.064
7.086 ± 0.045
0.902 ± 0.019
3.463 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here