Hubei mosquito virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFE1|A0A1L3KFE1_9VIRU Uncharacterized protein OS=Hubei mosquito virus 4 OX=1922928 PE=4 SV=1
MM1 pKa = 7.79KK2 pKa = 10.13GRR4 pKa = 11.84AISSSQLPGSSLRR17 pKa = 11.84TGEE20 pKa = 4.77CGHH23 pKa = 5.42SCKK26 pKa = 10.43RR27 pKa = 11.84YY28 pKa = 6.36TRR30 pKa = 11.84KK31 pKa = 9.2MFDD34 pKa = 3.12YY35 pKa = 9.18DD36 pKa = 3.39TDD38 pKa = 4.03FGGSGSVVKK47 pKa = 8.78THH49 pKa = 6.91LSCVCNEE56 pKa = 3.83VLALMNRR63 pKa = 11.84HH64 pKa = 5.58QLDD67 pKa = 3.43DD68 pKa = 3.69GARR71 pKa = 11.84YY72 pKa = 7.66TFTGDD77 pKa = 2.76LTKK80 pKa = 9.92WVRR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84VRR87 pKa = 11.84PMYY90 pKa = 9.36PWSEE94 pKa = 4.3SMVIEE99 pKa = 4.47HH100 pKa = 7.78AIPSKK105 pKa = 10.76KK106 pKa = 9.96KK107 pKa = 10.21LLISAKK113 pKa = 9.76EE114 pKa = 4.06SLSKK118 pKa = 11.28YY119 pKa = 9.88PLTASDD125 pKa = 4.12GAIKK129 pKa = 10.18MFLKK133 pKa = 10.34DD134 pKa = 3.72DD135 pKa = 4.18KK136 pKa = 11.71YY137 pKa = 10.79HH138 pKa = 7.37GEE140 pKa = 4.22LKK142 pKa = 10.53DD143 pKa = 3.65PRR145 pKa = 11.84CIQYY149 pKa = 10.24RR150 pKa = 11.84NKK152 pKa = 10.62RR153 pKa = 11.84YY154 pKa = 9.68GLRR157 pKa = 11.84LATYY161 pKa = 7.44LHH163 pKa = 6.68PLEE166 pKa = 5.23KK167 pKa = 10.73YY168 pKa = 9.5VMSWTRR174 pKa = 11.84NGTHH178 pKa = 6.79IFAKK182 pKa = 9.08GRR184 pKa = 11.84NMRR187 pKa = 11.84QRR189 pKa = 11.84GRR191 pKa = 11.84DD192 pKa = 3.06IAHH195 pKa = 6.92KK196 pKa = 9.78FNRR199 pKa = 11.84YY200 pKa = 9.03DD201 pKa = 3.15EE202 pKa = 4.62CAVVSLDD209 pKa = 3.44HH210 pKa = 7.25SKK212 pKa = 10.71FDD214 pKa = 3.54CHH216 pKa = 7.29VNEE219 pKa = 4.09SLLRR223 pKa = 11.84AEE225 pKa = 3.93HH226 pKa = 6.41WFYY229 pKa = 10.27NQCYY233 pKa = 9.52GSEE236 pKa = 3.98EE237 pKa = 4.51LEE239 pKa = 4.32FLLEE243 pKa = 3.89LQVRR247 pKa = 11.84NKK249 pKa = 10.53GMTKK253 pKa = 10.41NGTKK257 pKa = 8.92YY258 pKa = 5.78TTRR261 pKa = 11.84ATRR264 pKa = 11.84MSGDD268 pKa = 3.33QNTGLGNCLINYY280 pKa = 7.48MMTKK284 pKa = 10.0QLLSKK289 pKa = 10.84LGIGYY294 pKa = 9.68DD295 pKa = 3.83LYY297 pKa = 10.84IDD299 pKa = 4.76GDD301 pKa = 3.9DD302 pKa = 4.12FLIFMEE308 pKa = 4.68KK309 pKa = 9.78RR310 pKa = 11.84HH311 pKa = 5.94VPLIDD316 pKa = 3.64PSAYY320 pKa = 10.33SVFGMSTKK328 pKa = 10.51LEE330 pKa = 4.27SVAYY334 pKa = 8.82TIEE337 pKa = 4.49HH338 pKa = 6.67VDD340 pKa = 4.23FCQCRR345 pKa = 11.84PVFDD349 pKa = 3.97GVGYY353 pKa = 9.2TMVRR357 pKa = 11.84NPEE360 pKa = 3.78RR361 pKa = 11.84MLARR365 pKa = 11.84LPWIVGPVEE374 pKa = 4.04DD375 pKa = 4.98SRR377 pKa = 11.84ANDD380 pKa = 3.13IVFSTGQCEE389 pKa = 3.96IAQGLGLPIGQYY401 pKa = 9.48IGQRR405 pKa = 11.84MCEE408 pKa = 3.79RR409 pKa = 11.84GGKK412 pKa = 6.99MVRR415 pKa = 11.84IRR417 pKa = 11.84HH418 pKa = 5.37RR419 pKa = 11.84AWLEE423 pKa = 3.52KK424 pKa = 9.79MKK426 pKa = 10.25PGKK429 pKa = 9.45LQPIEE434 pKa = 3.79PMGGVRR440 pKa = 11.84EE441 pKa = 4.26SYY443 pKa = 9.9EE444 pKa = 4.21RR445 pKa = 11.84AWGLSIADD453 pKa = 3.66QLLIEE458 pKa = 4.3QTSLVEE464 pKa = 4.2PEE466 pKa = 3.81EE467 pKa = 4.23HH468 pKa = 6.38FAPGILQWW476 pKa = 3.74

Molecular weight: 54.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFL5|A0A1L3KFL5_9VIRU Uncharacterized protein OS=Hubei mosquito virus 4 OX=1922928 PE=4 SV=1
MM1 pKa = 7.0SQHH4 pKa = 6.3GARR7 pKa = 11.84GQPRR11 pKa = 11.84GAGRR15 pKa = 11.84PTPTSGHH22 pKa = 6.2RR23 pKa = 11.84GKK25 pKa = 10.49DD26 pKa = 3.34GSKK29 pKa = 10.29EE30 pKa = 3.93PKK32 pKa = 10.31GDD34 pKa = 3.44DD35 pKa = 3.18RR36 pKa = 11.84QRR38 pKa = 11.84NPRR41 pKa = 11.84SAKK44 pKa = 10.06GKK46 pKa = 8.7PSGNARR52 pKa = 11.84GPQWQTVGRR61 pKa = 11.84KK62 pKa = 8.51PRR64 pKa = 11.84AANPEE69 pKa = 3.78ALLRR73 pKa = 11.84RR74 pKa = 11.84IHH76 pKa = 7.0ALEE79 pKa = 3.7QANRR83 pKa = 11.84NLLLQLGRR91 pKa = 11.84VNAGASGSGSTSRR104 pKa = 11.84GSTSGRR110 pKa = 11.84TNPASPARR118 pKa = 11.84PKK120 pKa = 9.87PAPRR124 pKa = 11.84TKK126 pKa = 10.7APAPAPRR133 pKa = 11.84TKK135 pKa = 10.77APAPPQRR142 pKa = 11.84PKK144 pKa = 11.07GPRR147 pKa = 11.84KK148 pKa = 9.31PLVRR152 pKa = 11.84GPGNRR157 pKa = 11.84TVSSSHH163 pKa = 6.13LPAVGTKK170 pKa = 10.47NSFQLLGEE178 pKa = 4.39EE179 pKa = 4.8FPHH182 pKa = 7.31LSRR185 pKa = 11.84GTLALAVSAGLSGVCTPTATIANGVQAEE213 pKa = 4.21ASTSGRR219 pKa = 11.84SPRR222 pKa = 11.84STRR225 pKa = 11.84RR226 pKa = 11.84ANSSPEE232 pKa = 3.9LNVRR236 pKa = 11.84RR237 pKa = 11.84PAPRR241 pKa = 11.84AEE243 pKa = 4.11GQRR246 pKa = 11.84RR247 pKa = 11.84SPASRR252 pKa = 11.84TNSIPTQGSSSQHH265 pKa = 5.44RR266 pKa = 11.84AATAEE271 pKa = 4.36PKK273 pKa = 9.99VAPNPAGSDD282 pKa = 3.16RR283 pKa = 11.84VPQPRR288 pKa = 11.84LRR290 pKa = 11.84MKK292 pKa = 10.55PGDD295 pKa = 4.32HH296 pKa = 6.19GWKK299 pKa = 10.09GAGPPGVFHH308 pKa = 7.06SPTVVSPRR316 pKa = 11.84SAGVCLEE323 pKa = 3.92VSAKK327 pKa = 10.32KK328 pKa = 10.9GEE330 pKa = 4.22LVEE333 pKa = 4.13NTNPVFVHH341 pKa = 6.57KK342 pKa = 10.19ATVRR346 pKa = 11.84KK347 pKa = 9.84DD348 pKa = 3.43VEE350 pKa = 4.64CGVGTTRR357 pKa = 11.84ANLAKK362 pKa = 10.75LLTTKK367 pKa = 9.73EE368 pKa = 3.79VSMAEE373 pKa = 4.59LYY375 pKa = 10.57RR376 pKa = 11.84DD377 pKa = 3.29ATVPMSSWKK386 pKa = 9.1DD387 pKa = 3.36TFGITIDD394 pKa = 3.34AAKK397 pKa = 10.54ALVVDD402 pKa = 4.03EE403 pKa = 4.22FLYY406 pKa = 11.17YY407 pKa = 10.52EE408 pKa = 4.19LVSRR412 pKa = 11.84FPFAVRR418 pKa = 11.84TADD421 pKa = 3.66LARR424 pKa = 11.84KK425 pKa = 7.97MFHH428 pKa = 6.87HH429 pKa = 7.23LNTVLAQYY437 pKa = 10.66DD438 pKa = 3.91CRR440 pKa = 11.84LYY442 pKa = 9.74TAKK445 pKa = 10.35EE446 pKa = 3.94LYY448 pKa = 9.76KK449 pKa = 10.69LKK451 pKa = 10.5QATVRR456 pKa = 11.84AALLPPAEE464 pKa = 4.24EE465 pKa = 4.01LLTRR469 pKa = 11.84KK470 pKa = 10.16LIQQEE475 pKa = 3.67ASQMEE480 pKa = 5.01KK481 pKa = 10.74YY482 pKa = 10.92NKK484 pKa = 9.95FADD487 pKa = 3.72KK488 pKa = 11.0GDD490 pKa = 3.94AGSVCRR496 pKa = 11.84PGSATASAFSSLLQSRR512 pKa = 11.84VGLRR516 pKa = 11.84PTPKK520 pKa = 10.41

Molecular weight: 55.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1396

400

520

465.3

51.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.166 ± 2.07

1.504 ± 0.549

4.155 ± 0.75

5.014 ± 0.528

3.51 ± 0.747

8.453 ± 0.098

2.077 ± 0.516

3.08 ± 1.096

5.946 ± 0.23

7.951 ± 0.579

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.865

3.725 ± 0.287

7.02 ± 1.635

3.653 ± 0.093

8.309 ± 0.723

8.453 ± 0.767

6.375 ± 0.685

6.304 ± 0.53

1.146 ± 0.313

2.722 ± 0.929
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski