Aeromonas phage phiARM81ld

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9KF71|A0A1I9KF71_9CAUD Rz lysis protein OS=Aeromonas phage phiARM81ld OX=1748773 GN=ARM81ld_p59 PE=4 SV=1
MM1 pKa = 7.77WFPDD5 pKa = 2.94GRR7 pKa = 11.84NRR9 pKa = 11.84QGGFTQNDD17 pKa = 4.47DD18 pKa = 3.72YY19 pKa = 11.95DD20 pKa = 3.74VDD22 pKa = 4.3YY23 pKa = 11.24INYY26 pKa = 7.3EE27 pKa = 4.28CPSCGYY33 pKa = 9.73KK34 pKa = 10.28FCDD37 pKa = 3.01II38 pKa = 4.6

Molecular weight: 4.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9KF37|A0A1I9KF37_9CAUD Uncharacterized protein OS=Aeromonas phage phiARM81ld OX=1748773 GN=ARM81ld_p17 PE=4 SV=1
MM1 pKa = 8.08LGMDD5 pKa = 3.3RR6 pKa = 11.84RR7 pKa = 11.84TGRR10 pKa = 11.84FISGTEE16 pKa = 3.78QLASRR21 pKa = 11.84LLQVFTTQLSSRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84LFGSNVPDD45 pKa = 3.61ALARR49 pKa = 11.84LTNQALVLQVKK60 pKa = 10.71AEE62 pKa = 4.16MFDD65 pKa = 4.68AMLNPANGVLDD76 pKa = 4.61FKK78 pKa = 9.78PTRR81 pKa = 11.84IQFEE85 pKa = 4.52VVTDD89 pKa = 4.14GLHH92 pKa = 5.2AHH94 pKa = 7.05IDD96 pKa = 3.88GRR98 pKa = 11.84WRR100 pKa = 11.84GSDD103 pKa = 2.57ITVRR107 pKa = 11.84VPIYY111 pKa = 10.4DD112 pKa = 3.3QQ113 pKa = 3.56

Molecular weight: 12.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

14706

38

1202

241.1

26.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.649 ± 0.58

1.149 ± 0.143

6.147 ± 0.18

6.378 ± 0.307

2.965 ± 0.182

8.228 ± 0.32

2.06 ± 0.188

4.971 ± 0.195

5.318 ± 0.296

9.044 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.012 ± 0.154

3.264 ± 0.252

4.556 ± 0.254

4.168 ± 0.212

6.324 ± 0.28

5.664 ± 0.277

5.46 ± 0.248

6.474 ± 0.252

1.632 ± 0.132

2.536 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski