Mycolicibacterium anyangense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5421 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N4W9W8|A0A6N4W9W8_9MYCO ESX-2 secretion system ATPase EccB2 OS=Mycolicibacterium anyangense OX=1431246 GN=eccB2 PE=3 SV=1
MM1 pKa = 7.09VRR3 pKa = 11.84SLHH6 pKa = 6.43AAPQASISLPTIPSGVAGLALTSAVEE32 pKa = 3.93LALAATVSVINALAPSPTRR51 pKa = 11.84VSSTLDD57 pKa = 3.47LNGDD61 pKa = 3.89TVIASSPEE69 pKa = 3.94QVTSFYY75 pKa = 11.21GRR77 pKa = 11.84WTYY80 pKa = 11.29LPGAPSVVQGEE91 pKa = 4.24QQFAVVDD98 pKa = 4.43PKK100 pKa = 10.83TGTSAGSFDD109 pKa = 3.97ALVSRR114 pKa = 11.84GNGYY118 pKa = 10.59NYY120 pKa = 10.34TEE122 pKa = 4.63LLVTSDD128 pKa = 3.31EE129 pKa = 4.32GSNVGTGAGQLPPVGSLISSFGIGPVTLSYY159 pKa = 10.07TAIPTGSGDD168 pKa = 3.39KK169 pKa = 10.97VSFRR173 pKa = 11.84LVTPLGSLALPMPFDD188 pKa = 3.88AAAGIADD195 pKa = 3.85YY196 pKa = 11.1SVDD199 pKa = 3.52SRR201 pKa = 11.84PVQLTNGYY209 pKa = 9.46SIAPAVSTPEE219 pKa = 4.25TYY221 pKa = 10.15TGTSGILPFFTTVQGRR237 pKa = 11.84QVFTIDD243 pKa = 3.3NPAGVPVGSFEE254 pKa = 4.75GVVTTTADD262 pKa = 2.9ILGTYY267 pKa = 7.26TQAILVTANDD277 pKa = 4.14GTNVGLDD284 pKa = 3.6PGQVPPVGSVYY295 pKa = 10.65NVVYY299 pKa = 10.61SGSDD303 pKa = 3.14QNYY306 pKa = 8.44VLYY309 pKa = 10.96SSMPAQSGDD318 pKa = 3.46PVAVSQVSSGAVATSDD334 pKa = 3.62LTLIDD339 pKa = 4.84ASEE342 pKa = 4.2QPATQPFTGPNGTTFVPVSTLQPTGVNGLPPRR374 pKa = 11.84EE375 pKa = 3.98VQIQGYY381 pKa = 7.14QQFAVYY387 pKa = 10.03DD388 pKa = 3.77SAGTRR393 pKa = 11.84IGSVDD398 pKa = 3.64ADD400 pKa = 4.37VYY402 pKa = 10.17SQWDD406 pKa = 3.49LLGIRR411 pKa = 11.84SQAILVTDD419 pKa = 3.83VTAGTTGTSAGQVPAVGSEE438 pKa = 3.96FNVVDD443 pKa = 4.62FGTSGFATADD453 pKa = 3.5SVTPTPTGDD462 pKa = 3.36VNSFSILTPVGNSPTLSIYY481 pKa = 10.59TKK483 pKa = 9.01VADD486 pKa = 4.03RR487 pKa = 11.84AGVTFADD494 pKa = 4.31PFTTVV499 pKa = 2.52

Molecular weight: 50.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N4W950|A0A6N4W950_9MYCO HTH tetR-type domain-containing protein OS=Mycolicibacterium anyangense OX=1431246 GN=MANY_20140 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.38GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5421

0

5421

1769607

40

4157

326.4

34.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.115 ± 0.05

0.803 ± 0.009

6.189 ± 0.025

4.971 ± 0.033

3.065 ± 0.02

9.021 ± 0.058

2.163 ± 0.013

4.399 ± 0.022

2.148 ± 0.021

9.872 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.013

2.294 ± 0.02

5.834 ± 0.031

3.074 ± 0.019

6.881 ± 0.033

5.532 ± 0.023

6.187 ± 0.022

8.781 ± 0.033

1.506 ± 0.013

2.158 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski