Amino acid dipepetide frequency for Flavobacteriaceae bacterium R33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.25AlaAla: 5.25 ± 0.098	0.612AlaCys: 0.612 ± 0.025	4.062AlaAsp: 4.062 ± 0.065	4.559AlaGlu: 4.559 ± 0.072	3.586AlaPhe: 3.586 ± 0.067	4.811AlaGly: 4.811 ± 0.085	1.204AlaHis: 1.204 ± 0.035	5.005AlaIle: 5.005 ± 0.067	4.056AlaLys: 4.056 ± 0.072	7.015AlaLeu: 7.015 ± 0.09	1.658AlaMet: 1.658 ± 0.037	3.328AlaAsn: 3.328 ± 0.054	2.237AlaPro: 2.237 ± 0.06	2.538AlaGln: 2.538 ± 0.044	2.789AlaArg: 2.789 ± 0.046	4.66AlaSer: 4.66 ± 0.102	3.891AlaThr: 3.891 ± 0.104	4.771AlaVal: 4.771 ± 0.061	0.772AlaTrp: 0.772 ± 0.025	2.626AlaTyr: 2.626 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.486CysAla: 0.486 ± 0.018	0.127CysCys: 0.127 ± 0.009	0.392CysAsp: 0.392 ± 0.018	0.457CysGlu: 0.457 ± 0.021	0.387CysPhe: 0.387 ± 0.019	0.682CysGly: 0.682 ± 0.028	0.184CysHis: 0.184 ± 0.013	0.511CysIle: 0.511 ± 0.021	0.435CysLys: 0.435 ± 0.02	0.7CysLeu: 0.7 ± 0.025	0.157CysMet: 0.157 ± 0.01	0.352CysAsn: 0.352 ± 0.019	0.307CysPro: 0.307 ± 0.018	0.26CysGln: 0.26 ± 0.016	0.269CysArg: 0.269 ± 0.013	0.609CysSer: 0.609 ± 0.032	0.571CysThr: 0.571 ± 0.051	0.404CysVal: 0.404 ± 0.021	0.079CysTrp: 0.079 ± 0.008	0.292CysTyr: 0.292 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.892AspAla: 3.892 ± 0.077	0.407AspCys: 0.407 ± 0.017	3.015AspAsp: 3.015 ± 0.096	3.829AspGlu: 3.829 ± 0.069	3.626AspPhe: 3.626 ± 0.061	4.627AspGly: 4.627 ± 0.11	1.18AspHis: 1.18 ± 0.034	4.326AspIle: 4.326 ± 0.077	3.404AspLys: 3.404 ± 0.061	5.885AspLeu: 5.885 ± 0.093	1.301AspMet: 1.301 ± 0.044	2.922AspAsn: 2.922 ± 0.081	2.594AspPro: 2.594 ± 0.056	2.332AspGln: 2.332 ± 0.107	2.393AspArg: 2.393 ± 0.046	3.597AspSer: 3.597 ± 0.053	2.904AspThr: 2.904 ± 0.077	3.481AspVal: 3.481 ± 0.088	0.848AspTrp: 0.848 ± 0.026	2.704AspTyr: 2.704 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.02GluAla: 5.02 ± 0.075	0.326GluCys: 0.326 ± 0.017	4.107GluAsp: 4.107 ± 0.062	5.498GluGlu: 5.498 ± 0.096	2.877GluPhe: 2.877 ± 0.048	4.289GluGly: 4.289 ± 0.067	1.207GluHis: 1.207 ± 0.037	5.146GluIle: 5.146 ± 0.063	4.977GluLys: 4.977 ± 0.084	6.777GluLeu: 6.777 ± 0.1	1.582GluMet: 1.582 ± 0.042	4.018GluAsn: 4.018 ± 0.06	1.849GluPro: 1.849 ± 0.044	2.522GluGln: 2.522 ± 0.05	3.01GluArg: 3.01 ± 0.056	3.403GluSer: 3.403 ± 0.056	3.519GluThr: 3.519 ± 0.059	4.905GluVal: 4.905 ± 0.072	0.697GluTrp: 0.697 ± 0.026	2.276GluTyr: 2.276 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.122PheAla: 3.122 ± 0.048	0.429PheCys: 0.429 ± 0.019	3.288PheAsp: 3.288 ± 0.061	3.421PheGlu: 3.421 ± 0.058	2.631PhePhe: 2.631 ± 0.052	3.728PheGly: 3.728 ± 0.062	0.849PheHis: 0.849 ± 0.026	3.158PheIle: 3.158 ± 0.056	2.947PheLys: 2.947 ± 0.068	4.819PheLeu: 4.819 ± 0.089	1.105PheMet: 1.105 ± 0.028	2.888PheAsn: 2.888 ± 0.077	1.824PhePro: 1.824 ± 0.036	1.622PheGln: 1.622 ± 0.03	2.1PheArg: 2.1 ± 0.038	3.824PheSer: 3.824 ± 0.062	2.825PheThr: 2.825 ± 0.046	3.078PheVal: 3.078 ± 0.051	0.653PheTrp: 0.653 ± 0.023	2.053PheTyr: 2.053 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.641GlyAla: 4.641 ± 0.086	0.735GlyCys: 0.735 ± 0.043	4.089GlyAsp: 4.089 ± 0.105	4.185GlyGlu: 4.185 ± 0.06	3.833GlyPhe: 3.833 ± 0.06	4.99GlyGly: 4.99 ± 0.097	1.252GlyHis: 1.252 ± 0.032	5.518GlyIle: 5.518 ± 0.082	4.744GlyLys: 4.744 ± 0.077	6.743GlyLeu: 6.743 ± 0.094	1.809GlyMet: 1.809 ± 0.043	3.937GlyAsn: 3.937 ± 0.1	1.826GlyPro: 1.826 ± 0.054	2.271GlyGln: 2.271 ± 0.04	2.644GlyArg: 2.644 ± 0.053	4.967GlySer: 4.967 ± 0.105	4.512GlyThr: 4.512 ± 0.197	4.436GlyVal: 4.436 ± 0.064	0.95GlyTrp: 0.95 ± 0.03	2.867GlyTyr: 2.867 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.03HisAla: 1.03 ± 0.033	0.173HisCys: 0.173 ± 0.011	0.762HisAsp: 0.762 ± 0.026	0.964HisGlu: 0.964 ± 0.032	1.137HisPhe: 1.137 ± 0.028	1.238HisGly: 1.238 ± 0.032	0.513HisHis: 0.513 ± 0.023	1.282HisIle: 1.282 ± 0.036	1.091HisLys: 1.091 ± 0.03	1.909HisLeu: 1.909 ± 0.05	0.413HisMet: 0.413 ± 0.016	0.787HisAsn: 0.787 ± 0.027	0.966HisPro: 0.966 ± 0.028	0.753HisGln: 0.753 ± 0.023	0.842HisArg: 0.842 ± 0.025	1.135HisSer: 1.135 ± 0.028	0.97HisThr: 0.97 ± 0.025	0.888HisVal: 0.888 ± 0.024	0.289HisTrp: 0.289 ± 0.015	0.838HisTyr: 0.838 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.283IleAla: 5.283 ± 0.071	0.578IleCys: 0.578 ± 0.019	4.186IleAsp: 4.186 ± 0.069	4.429IleGlu: 4.429 ± 0.075	3.108IlePhe: 3.108 ± 0.049	4.95IleGly: 4.95 ± 0.075	1.236IleHis: 1.236 ± 0.037	4.244IleIle: 4.244 ± 0.073	3.98IleLys: 3.98 ± 0.075	6.391IleLeu: 6.391 ± 0.106	1.216IleMet: 1.216 ± 0.031	3.521IleAsn: 3.521 ± 0.048	3.234IlePro: 3.234 ± 0.052	2.365IleGln: 2.365 ± 0.056	3.225IleArg: 3.225 ± 0.054	5.49IleSer: 5.49 ± 0.065	4.372IleThr: 4.372 ± 0.119	4.01IleVal: 4.01 ± 0.065	0.766IleTrp: 0.766 ± 0.027	2.465IleTyr: 2.465 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.773LysAla: 4.773 ± 0.076	0.26LysCys: 0.26 ± 0.015	3.843LysAsp: 3.843 ± 0.075	5.245LysGlu: 5.245 ± 0.097	2.214LysPhe: 2.214 ± 0.047	4.222LysGly: 4.222 ± 0.083	1.116LysHis: 1.116 ± 0.033	4.32LysIle: 4.32 ± 0.089	5.068LysLys: 5.068 ± 0.109	5.679LysLeu: 5.679 ± 0.096	1.537LysMet: 1.537 ± 0.035	3.342LysAsn: 3.342 ± 0.065	2.219LysPro: 2.219 ± 0.046	2.169LysGln: 2.169 ± 0.048	2.891LysArg: 2.891 ± 0.06	3.733LysSer: 3.733 ± 0.053	3.571LysThr: 3.571 ± 0.056	4.001LysVal: 4.001 ± 0.073	0.743LysTrp: 0.743 ± 0.022	2.317LysTyr: 2.317 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	6.596LeuAla: 6.596 ± 0.086	0.756LeuCys: 0.756 ± 0.026	5.59LeuAsp: 5.59 ± 0.063	6.782LeuGlu: 6.782 ± 0.099	4.785LeuPhe: 4.785 ± 0.078	6.697LeuGly: 6.697 ± 0.089	1.599LeuHis: 1.599 ± 0.044	6.437LeuIle: 6.437 ± 0.092	6.869LeuLys: 6.869 ± 0.126	9.606LeuLeu: 9.606 ± 0.145	2.153LeuMet: 2.153 ± 0.052	5.076LeuAsn: 5.076 ± 0.064	3.803LeuPro: 3.803 ± 0.063	3.387LeuGln: 3.387 ± 0.051	4.102LeuArg: 4.102 ± 0.057	7.302LeuSer: 7.302 ± 0.098	5.05LeuThr: 5.05 ± 0.092	5.837LeuVal: 5.837 ± 0.077	1.03LeuTrp: 1.03 ± 0.03	3.146LeuTyr: 3.146 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.848MetAla: 1.848 ± 0.041	0.126MetCys: 0.126 ± 0.01	1.363MetAsp: 1.363 ± 0.031	1.674MetGlu: 1.674 ± 0.036	0.983MetPhe: 0.983 ± 0.084	1.645MetGly: 1.645 ± 0.04	0.401MetHis: 0.401 ± 0.015	1.314MetIle: 1.314 ± 0.035	1.822MetLys: 1.822 ± 0.043	1.985MetLeu: 1.985 ± 0.051	0.482MetMet: 0.482 ± 0.021	1.144MetAsn: 1.144 ± 0.032	0.861MetPro: 0.861 ± 0.028	0.832MetGln: 0.832 ± 0.021	1.063MetArg: 1.063 ± 0.03	1.364MetSer: 1.364 ± 0.034	1.076MetThr: 1.076 ± 0.027	1.432MetVal: 1.432 ± 0.032	0.18MetTrp: 0.18 ± 0.012	0.629MetTyr: 0.629 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.532AsnAla: 3.532 ± 0.057	0.491AsnCys: 0.491 ± 0.035	2.663AsnAsp: 2.663 ± 0.055	3.001AsnGlu: 3.001 ± 0.054	2.679AsnPhe: 2.679 ± 0.046	4.124AsnGly: 4.124 ± 0.107	0.886AsnHis: 0.886 ± 0.026	3.609AsnIle: 3.609 ± 0.067	2.807AsnLys: 2.807 ± 0.052	4.608AsnLeu: 4.608 ± 0.064	1.23AsnMet: 1.23 ± 0.049	2.693AsnAsn: 2.693 ± 0.062	2.762AsnPro: 2.762 ± 0.072	1.883AsnGln: 1.883 ± 0.044	2.284AsnArg: 2.284 ± 0.039	3.677AsnSer: 3.677 ± 0.089	3.491AsnThr: 3.491 ± 0.13	2.941AsnVal: 2.941 ± 0.064	0.777AsnTrp: 0.777 ± 0.03	2.294AsnTyr: 2.294 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	2.439ProAla: 2.439 ± 0.06	0.196ProCys: 0.196 ± 0.011	2.695ProAsp: 2.695 ± 0.051	3.673ProGlu: 3.673 ± 0.057	1.934ProPhe: 1.934 ± 0.042	2.616ProGly: 2.616 ± 0.048	0.653ProHis: 0.653 ± 0.024	2.42ProIle: 2.42 ± 0.051	2.26ProLys: 2.26 ± 0.048	3.356ProLeu: 3.356 ± 0.059	0.845ProMet: 0.845 ± 0.025	1.961ProAsn: 1.961 ± 0.039	1.183ProPro: 1.183 ± 0.043	1.238ProGln: 1.238 ± 0.042	1.274ProArg: 1.274 ± 0.034	2.222ProSer: 2.222 ± 0.042	2.086ProThr: 2.086 ± 0.087	2.57ProVal: 2.57 ± 0.045	0.458ProTrp: 0.458 ± 0.019	1.423ProTyr: 1.423 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.368GlnAla: 2.368 ± 0.047	0.27GlnCys: 0.27 ± 0.03	1.917GlnAsp: 1.917 ± 0.048	2.455GlnGlu: 2.455 ± 0.049	1.624GlnPhe: 1.624 ± 0.037	2.423GlnGly: 2.423 ± 0.151	0.612GlnHis: 0.612 ± 0.023	2.411GlnIle: 2.411 ± 0.04	2.507GlnLys: 2.507 ± 0.058	3.697GlnLeu: 3.697 ± 0.059	0.884GlnMet: 0.884 ± 0.025	1.977GlnAsn: 1.977 ± 0.058	1.167GlnPro: 1.167 ± 0.027	1.59GlnGln: 1.59 ± 0.037	1.533GlnArg: 1.533 ± 0.031	1.988GlnSer: 1.988 ± 0.035	1.909GlnThr: 1.909 ± 0.063	1.974GlnVal: 1.974 ± 0.035	0.479GlnTrp: 0.479 ± 0.022	1.301GlnTyr: 1.301 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.634ArgAla: 2.634 ± 0.049	0.223ArgCys: 0.223 ± 0.012	2.341ArgAsp: 2.341 ± 0.039	2.947ArgGlu: 2.947 ± 0.061	2.294ArgPhe: 2.294 ± 0.041	2.381ArgGly: 2.381 ± 0.045	0.758ArgHis: 0.758 ± 0.024	3.447ArgIle: 3.447 ± 0.058	3.124ArgLys: 3.124 ± 0.064	4.101ArgLeu: 4.101 ± 0.066	1.034ArgMet: 1.034 ± 0.028	2.317ArgAsn: 2.317 ± 0.04	1.487ArgPro: 1.487 ± 0.038	1.477ArgGln: 1.477 ± 0.033	1.894ArgArg: 1.894 ± 0.042	2.695ArgSer: 2.695 ± 0.05	2.255ArgThr: 2.255 ± 0.042	2.564ArgVal: 2.564 ± 0.046	0.551ArgTrp: 0.551 ± 0.02	1.834ArgTyr: 1.834 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.308SerAla: 4.308 ± 0.067	0.718SerCys: 0.718 ± 0.027	3.939SerAsp: 3.939 ± 0.096	4.339SerGlu: 4.339 ± 0.067	3.67SerPhe: 3.67 ± 0.053	5.736SerGly: 5.736 ± 0.139	1.108SerHis: 1.108 ± 0.033	4.507SerIle: 4.507 ± 0.059	3.893SerLys: 3.893 ± 0.065	6.533SerLeu: 6.533 ± 0.086	1.45SerMet: 1.45 ± 0.042	3.412SerAsn: 3.412 ± 0.111	2.406SerPro: 2.406 ± 0.046	2.1SerGln: 2.1 ± 0.039	2.872SerArg: 2.872 ± 0.054	4.576SerSer: 4.576 ± 0.098	3.491SerThr: 3.491 ± 0.066	4.169SerVal: 4.169 ± 0.08	1.091SerTrp: 1.091 ± 0.072	2.722SerTyr: 2.722 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	4.612ThrAla: 4.612 ± 0.166	0.307ThrCys: 0.307 ± 0.027	3.871ThrAsp: 3.871 ± 0.184	3.459ThrGlu: 3.459 ± 0.054	2.829ThrPhe: 2.829 ± 0.059	4.134ThrGly: 4.134 ± 0.083	0.971ThrHis: 0.971 ± 0.025	4.094ThrIle: 4.094 ± 0.113	2.726ThrLys: 2.726 ± 0.06	5.351ThrLeu: 5.351 ± 0.085	0.958ThrMet: 0.958 ± 0.028	2.794ThrAsn: 2.794 ± 0.12	2.397ThrPro: 2.397 ± 0.05	1.76ThrGln: 1.76 ± 0.043	2.115ThrArg: 2.115 ± 0.041	3.872ThrSer: 3.872 ± 0.129	3.498ThrThr: 3.498 ± 0.142	4.272ThrVal: 4.272 ± 0.164	0.56ThrTrp: 0.56 ± 0.026	2.345ThrTyr: 2.345 ± 0.083	0.0ThrXaa: 0.0 ± 0.0
Val	4.364ValAla: 4.364 ± 0.058	0.486ValCys: 0.486 ± 0.019	3.771ValAsp: 3.771 ± 0.087	3.795ValGlu: 3.795 ± 0.058	3.341ValPhe: 3.341 ± 0.053	4.043ValGly: 4.043 ± 0.066	1.112ValHis: 1.112 ± 0.031	4.257ValIle: 4.257 ± 0.062	3.548ValLys: 3.548 ± 0.069	6.485ValLeu: 6.485 ± 0.08	1.357ValMet: 1.357 ± 0.036	3.134ValAsn: 3.134 ± 0.056	2.507ValPro: 2.507 ± 0.042	1.978ValGln: 1.978 ± 0.04	2.541ValArg: 2.541 ± 0.046	4.699ValSer: 4.699 ± 0.084	4.147ValThr: 4.147 ± 0.239	4.397ValVal: 4.397 ± 0.066	0.651ValTrp: 0.651 ± 0.021	2.32ValTyr: 2.32 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.701TrpAla: 0.701 ± 0.026	0.091TrpCys: 0.091 ± 0.008	0.916TrpAsp: 0.916 ± 0.057	0.841TrpGlu: 0.841 ± 0.027	0.592TrpPhe: 0.592 ± 0.023	0.874TrpGly: 0.874 ± 0.031	0.285TrpHis: 0.285 ± 0.017	0.804TrpIle: 0.804 ± 0.023	0.797TrpLys: 0.797 ± 0.028	1.113TrpLeu: 1.113 ± 0.03	0.352TrpMet: 0.352 ± 0.017	0.819TrpAsn: 0.819 ± 0.068	0.329TrpPro: 0.329 ± 0.016	0.431TrpGln: 0.431 ± 0.017	0.545TrpArg: 0.545 ± 0.02	0.736TrpSer: 0.736 ± 0.025	0.631TrpThr: 0.631 ± 0.021	0.722TrpVal: 0.722 ± 0.024	0.185TrpTrp: 0.185 ± 0.012	0.511TrpTyr: 0.511 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.531TyrAla: 2.531 ± 0.05	0.351TyrCys: 0.351 ± 0.017	2.458TyrAsp: 2.458 ± 0.049	2.452TyrGlu: 2.452 ± 0.042	2.233TyrPhe: 2.233 ± 0.04	2.738TyrGly: 2.738 ± 0.049	0.853TyrHis: 0.853 ± 0.025	2.237TyrIle: 2.237 ± 0.043	2.206TyrLys: 2.206 ± 0.046	3.848TyrLeu: 3.848 ± 0.065	0.706TyrMet: 0.706 ± 0.024	2.011TyrAsn: 2.011 ± 0.039	1.545TyrPro: 1.545 ± 0.036	1.578TyrGln: 1.578 ± 0.041	1.973TyrArg: 1.973 ± 0.043	2.494TyrSer: 2.494 ± 0.045	2.154TyrThr: 2.154 ± 0.056	2.082TyrVal: 2.082 ± 0.041	0.515TyrTrp: 0.515 ± 0.02	1.684TyrTyr: 1.684 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4113 proteins (1474473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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