Mesorhizobium alhagi CCNWXJ12-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; Mesorhizobium alhagi

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0HTG2|H0HTG2_9RHIZ Peptidase M24 OS=Mesorhizobium alhagi CCNWXJ12-2 OX=1107882 GN=MAXJ12_17268 PE=3 SV=1
MM1 pKa = 6.81TTTITSNLTTQFNITAPNDD20 pKa = 3.06NWVLANGVSINVANGHH36 pKa = 6.45GIFNGGAYY44 pKa = 9.38HH45 pKa = 7.32DD46 pKa = 4.3SVVEE50 pKa = 4.05VRR52 pKa = 11.84GHH54 pKa = 4.82ITANATYY61 pKa = 10.16KK62 pKa = 10.78AGVYY66 pKa = 10.23SDD68 pKa = 4.24GLDD71 pKa = 4.19AILSVADD78 pKa = 4.4LGTIHH83 pKa = 7.68AYY85 pKa = 10.74NGMAFYY91 pKa = 11.22GDD93 pKa = 3.22GTAADD98 pKa = 3.42NHH100 pKa = 6.59GIITASNIGMYY111 pKa = 10.99GNGNDD116 pKa = 3.54IKK118 pKa = 10.54ISNFGSIVAEE128 pKa = 3.65AGIYY132 pKa = 9.92VEE134 pKa = 4.57AASNHH139 pKa = 4.84SQIEE143 pKa = 4.26NNGKK147 pKa = 9.04IEE149 pKa = 4.56GYY151 pKa = 10.26SGIISVNSRR160 pKa = 11.84LDD162 pKa = 3.12IKK164 pKa = 10.89LGSQSVIDD172 pKa = 3.75VQGIGIISQGQLTTYY187 pKa = 9.88VEE189 pKa = 4.16NYY191 pKa = 10.62GFIKK195 pKa = 10.36SANLAIKK202 pKa = 10.45GGDD205 pKa = 3.6GEE207 pKa = 5.67DD208 pKa = 3.14IVINYY213 pKa = 9.8GNIQGGIFLGGGNDD227 pKa = 3.5FFDD230 pKa = 4.02NRR232 pKa = 11.84TGTIDD237 pKa = 3.39HH238 pKa = 6.76AVDD241 pKa = 3.53GGLGDD246 pKa = 4.06DD247 pKa = 4.36YY248 pKa = 11.6YY249 pKa = 12.06VFGAKK254 pKa = 9.83PFIIIDD260 pKa = 3.45SGGNDD265 pKa = 3.75TIGTEE270 pKa = 4.05ITRR273 pKa = 11.84SLASYY278 pKa = 11.08GNIEE282 pKa = 4.24NLHH285 pKa = 6.18LWGSANINGTGNQLANVISGNSGNNILDD313 pKa = 3.8GGADD317 pKa = 3.62NIVDD321 pKa = 3.55QLEE324 pKa = 4.15GRR326 pKa = 11.84EE327 pKa = 4.24GNDD330 pKa = 3.24TYY332 pKa = 11.93VLGAGFDD339 pKa = 3.61IVIEE343 pKa = 4.54SIFPSAAFVGTVYY356 pKa = 10.9GGVDD360 pKa = 4.31TITSTISRR368 pKa = 11.84SLGDD372 pKa = 3.55YY373 pKa = 11.07AHH375 pKa = 6.61VEE377 pKa = 3.99NLKK380 pKa = 10.9LLGSANINGTGDD392 pKa = 3.1EE393 pKa = 4.28AANVITGNSGNNILNGGGGNDD414 pKa = 3.93TLNGGAGADD423 pKa = 3.41AMFGGTGDD431 pKa = 4.79DD432 pKa = 3.41IYY434 pKa = 11.01IVAAAGDD441 pKa = 4.0TTIEE445 pKa = 4.12NAGEE449 pKa = 4.08GTDD452 pKa = 3.6TVRR455 pKa = 11.84SYY457 pKa = 11.42IDD459 pKa = 3.02WVLANNVEE467 pKa = 4.08RR468 pKa = 11.84LEE470 pKa = 4.15LQGSGNLNGTGNALNNTLVGNSGNNLLNGGAGNDD504 pKa = 3.64YY505 pKa = 10.05MVGGGGDD512 pKa = 3.42DD513 pKa = 3.72TFIVAAAGDD522 pKa = 3.86ITVEE526 pKa = 4.21SAGGGTDD533 pKa = 3.27TVRR536 pKa = 11.84SYY538 pKa = 11.69INWTLGANVEE548 pKa = 4.09RR549 pKa = 11.84LEE551 pKa = 4.21LQGSSNLNGTGNSLNNTLVGNSGNNSLSGGDD582 pKa = 3.49GNDD585 pKa = 3.95YY586 pKa = 9.73ITGGAGNDD594 pKa = 3.61TLTGGVGNDD603 pKa = 3.67TLIGGAGSDD612 pKa = 4.11SLNGGAGNDD621 pKa = 3.17RR622 pKa = 11.84FDD624 pKa = 4.56FDD626 pKa = 5.06LVSHH630 pKa = 6.42SPVGPALRR638 pKa = 11.84DD639 pKa = 3.76SIVGGFSHH647 pKa = 6.99GFDD650 pKa = 5.2LIDD653 pKa = 5.12LATIDD658 pKa = 4.92ANTLVAGNQAFSFIGSAAFSGAAGQLRR685 pKa = 11.84YY686 pKa = 8.45TNYY689 pKa = 10.23NGTVIIDD696 pKa = 3.49ADD698 pKa = 3.99VNGDD702 pKa = 4.38SIADD706 pKa = 3.77MQILVAGTSFMTGTDD721 pKa = 3.75FVLL724 pKa = 4.53

Molecular weight: 73.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0HNS7|H0HNS7_9RHIZ Uncharacterized protein OS=Mesorhizobium alhagi CCNWXJ12-2 OX=1107882 GN=MAXJ12_09011 PE=4 SV=1
MM1 pKa = 7.48IAPVRR6 pKa = 11.84LPLRR10 pKa = 11.84VAPGHH15 pKa = 5.71RR16 pKa = 11.84QAVTAPQQAKK26 pKa = 8.75TIWLVRR32 pKa = 11.84MFSPNFATFPLVGRR46 pKa = 11.84RR47 pKa = 11.84QALRR51 pKa = 3.46

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7195

0

7195

1987422

19

5515

276.2

30.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.38 ± 0.039

0.83 ± 0.011

5.67 ± 0.026

5.996 ± 0.03

3.919 ± 0.017

8.603 ± 0.043

2.011 ± 0.016

5.421 ± 0.024

3.465 ± 0.03

9.789 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.016

2.712 ± 0.023

5.071 ± 0.025

2.958 ± 0.019

7.068 ± 0.033

5.553 ± 0.022

5.156 ± 0.033

7.317 ± 0.022

1.353 ± 0.012

2.192 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski