Commensalibacter sp. MX01
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W7DYL5|W7DYL5_9PROT AFG1 family ATPase OS=Commensalibacter sp. MX01 OX=1208583 GN=COMX_07290 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 5.36 NNDD5 pKa = 3.36 ANEE8 pKa = 4.29 NYY10 pKa = 10.08 VFDD13 pKa = 5.64 PDD15 pKa = 4.33 SEE17 pKa = 4.86 SVDD20 pKa = 3.18 PDD22 pKa = 3.31 QPFEE26 pKa = 4.19 VGSDD30 pKa = 3.59 FFDD33 pKa = 3.77 NPITKK38 pKa = 9.54 EE39 pKa = 3.76 ASVKK43 pKa = 10.46 FFGDD47 pKa = 2.97 LRR49 pKa = 11.84 DD50 pKa = 4.18 YY51 pKa = 9.9 ITSGNVKK58 pKa = 10.39 DD59 pKa = 5.1 VVLEE63 pKa = 4.2 PNTHH67 pKa = 5.93 IDD69 pKa = 4.03 ANEE72 pKa = 3.92 LDD74 pKa = 3.93 YY75 pKa = 11.52 TPIVTMYY82 pKa = 10.68 SFSDD86 pKa = 3.63 SQNIFYY92 pKa = 10.76 LSTGKK97 pKa = 10.24 FPQDD101 pKa = 2.75 TEE103 pKa = 4.31 YY104 pKa = 11.65 AEE106 pKa = 4.34 VTLGPGQTTDD116 pKa = 4.06 NINGIPKK123 pKa = 9.76 IQGDD127 pKa = 4.52 GNNPNYY133 pKa = 10.27 VRR135 pKa = 11.84 IIGGVDD141 pKa = 3.52 FNYY144 pKa = 10.3 IADD147 pKa = 4.21 GEE149 pKa = 4.43 SGEE152 pKa = 5.05 LISNNPLYY160 pKa = 10.54 FYY162 pKa = 11.37 GRR164 pKa = 11.84 GLDD167 pKa = 3.67 GNTSHH172 pKa = 6.6 MGNWTIYY179 pKa = 9.98 TDD181 pKa = 4.74 DD182 pKa = 4.69 FVDD185 pKa = 4.28 PDD187 pKa = 3.5 TGEE190 pKa = 4.37 IYY192 pKa = 10.85 DD193 pKa = 4.59 DD194 pKa = 5.35 DD195 pKa = 6.29 RR196 pKa = 11.84 DD197 pKa = 4.01 TMIQTTDD204 pKa = 3.28 GNNKK208 pKa = 7.94 ITTGYY213 pKa = 10.49 ARR215 pKa = 11.84 STIDD219 pKa = 4.11 LGSGNNIVYY228 pKa = 10.24 SQGHH232 pKa = 5.08 DD233 pKa = 3.52 TITSSRR239 pKa = 11.84 GGYY242 pKa = 7.18 QTVTLTGSQSKK253 pKa = 8.33 LTMGGNTTILDD264 pKa = 3.34 IGNNNNISVGKK275 pKa = 9.9 NSSVYY280 pKa = 10.26 GAASDD285 pKa = 3.66 IVQFDD290 pKa = 3.49 TGDD293 pKa = 3.61 MNEE296 pKa = 4.06 YY297 pKa = 9.16 TAGNNSTISATNGTDD312 pKa = 3.04 LKK314 pKa = 10.97 VIHH317 pKa = 6.59 GLDD320 pKa = 2.92 NTYY323 pKa = 10.93 NINHH327 pKa = 6.76 NLTFLNGSGNTVINIKK343 pKa = 9.05 GTLAAFGAGSSQYY356 pKa = 10.08 TLNAEE361 pKa = 4.71 NIDD364 pKa = 3.98 STSGSIFVANEE375 pKa = 3.63 GNEE378 pKa = 4.23 TLDD381 pKa = 4.04 AASSSANLQIWANTVAGANNLLAKK405 pKa = 10.31 AGQGDD410 pKa = 3.94 DD411 pKa = 3.43 TLAAGVGNATFTGGVGNNLFMFTKK435 pKa = 10.41 EE436 pKa = 4.12 SVQDD440 pKa = 3.46 GTTIITDD447 pKa = 3.94 FTNNDD452 pKa = 3.27 KK453 pKa = 10.8 IALYY457 pKa = 10.58 NYY459 pKa = 10.17 GLGSNGLTQILQQSQNDD476 pKa = 3.77 ANGNAVLKK484 pKa = 8.64 LTNNAAIVIEE494 pKa = 4.32 GVSVSSLSTKK504 pKa = 10.36 QFDD507 pKa = 3.56 IADD510 pKa = 4.34 FSHH513 pKa = 6.85
Molecular weight: 54.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.63
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.821
Sillero 3.986
Patrickios 2.435
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|W7DVK7|W7DVK7_9PROT Methionine aminopeptidase OS=Commensalibacter sp. MX01 OX=1208583 GN=map PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPSKK9 pKa = 9.06 IVRR12 pKa = 11.84 KK13 pKa = 9.03 HH14 pKa = 4.06 RR15 pKa = 11.84 HH16 pKa = 4.08 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.49 VITNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.87 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2060
0
2060
686148
41
4383
333.1
37.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.028 ± 0.065
1.114 ± 0.02
5.163 ± 0.041
5.422 ± 0.059
4.262 ± 0.044
6.91 ± 0.071
2.27 ± 0.027
7.752 ± 0.051
5.968 ± 0.045
9.948 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.026
4.763 ± 0.076
4.38 ± 0.038
4.592 ± 0.049
4.366 ± 0.044
6.389 ± 0.046
5.428 ± 0.048
6.262 ± 0.04
1.292 ± 0.022
3.234 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here