Steroidobacter denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Steroidobacteraceae; Steroidobacter

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127FEE6|A0A127FEE6_STEDE Antitoxin ParD OS=Steroidobacter denitrificans OX=465721 GN=ACG33_14680 PE=3 SV=1
MM1 pKa = 7.35SGAAGEE7 pKa = 4.33EE8 pKa = 4.26TVQRR12 pKa = 11.84VWEE15 pKa = 4.05ALRR18 pKa = 11.84VVIDD22 pKa = 4.02PEE24 pKa = 4.08LGYY27 pKa = 11.13NIVDD31 pKa = 4.13LGLIYY36 pKa = 10.82DD37 pKa = 3.63VDD39 pKa = 4.27VNAEE43 pKa = 4.01GAAHH47 pKa = 7.27IMMTTTTRR55 pKa = 11.84GCPATHH61 pKa = 6.34YY62 pKa = 9.4LQTGAYY68 pKa = 7.13EE69 pKa = 4.37AALSTDD75 pKa = 4.89DD76 pKa = 3.35ITSAEE81 pKa = 4.08VEE83 pKa = 4.28MTYY86 pKa = 10.84DD87 pKa = 3.83PPWNPGMMRR96 pKa = 11.84PEE98 pKa = 4.0AKK100 pKa = 10.18AHH102 pKa = 6.44FGIPDD107 pKa = 3.55DD108 pKa = 3.92

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127F5I7|A0A127F5I7_STEDE Uncharacterized protein OS=Steroidobacter denitrificans OX=465721 GN=ACG33_01000 PE=4 SV=1
MM1 pKa = 6.38ATKK4 pKa = 10.41RR5 pKa = 11.84KK6 pKa = 9.4VSKK9 pKa = 10.37KK10 pKa = 9.58KK11 pKa = 10.33AGAKK15 pKa = 9.82RR16 pKa = 11.84KK17 pKa = 8.31VAKK20 pKa = 9.28TAVKK24 pKa = 10.33KK25 pKa = 10.55KK26 pKa = 8.81VARR29 pKa = 11.84TAAKK33 pKa = 10.03KK34 pKa = 10.1KK35 pKa = 8.91VAKK38 pKa = 10.32APRR41 pKa = 11.84KK42 pKa = 8.14VAKK45 pKa = 10.38KK46 pKa = 9.77KK47 pKa = 8.93VAKK50 pKa = 9.19TAVKK54 pKa = 10.33KK55 pKa = 10.55KK56 pKa = 8.81VARR59 pKa = 11.84TAAKK63 pKa = 10.03KK64 pKa = 10.1KK65 pKa = 8.91VAKK68 pKa = 10.32APRR71 pKa = 11.84KK72 pKa = 8.33VAKK75 pKa = 10.07KK76 pKa = 10.0KK77 pKa = 10.76AGAKK81 pKa = 9.77KK82 pKa = 9.77SAAKK86 pKa = 9.85RR87 pKa = 11.84RR88 pKa = 11.84SASVVRR94 pKa = 11.84APQQ97 pKa = 3.08

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2956

0

2956

988673

41

2117

334.5

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.818 ± 0.051

0.972 ± 0.017

5.498 ± 0.026

5.923 ± 0.042

3.444 ± 0.03

8.08 ± 0.035

2.303 ± 0.021

5.108 ± 0.032

2.771 ± 0.032

10.738 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.021

2.496 ± 0.024

5.014 ± 0.027

3.864 ± 0.03

8.166 ± 0.038

5.496 ± 0.03

5.015 ± 0.026

7.143 ± 0.035

1.338 ± 0.021

2.437 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski