Sanxia permutotetra-like virus 1
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHX9|A0A1L3KHX9_9VIRU Capsid protein OS=Sanxia permutotetra-like virus 1 OX=1923365 PE=3 SV=1
MM1 pKa = 7.05 TANALDD7 pKa = 3.86 GAAAKK12 pKa = 10.57 SKK14 pKa = 10.85 GPDD17 pKa = 3.99 PIQTLHH23 pKa = 7.2 LGDD26 pKa = 3.68 NTLSLEE32 pKa = 4.26 SRR34 pKa = 11.84 NTPAFDD40 pKa = 4.14 NISRR44 pKa = 11.84 INLGLDD50 pKa = 2.99 TFLTIRR56 pKa = 11.84 TNFTLNAGDD65 pKa = 3.58 ITSVVCSLKK74 pKa = 10.96 SSDD77 pKa = 4.07 GIPWNIPWTGVPVVDD92 pKa = 5.51 KK93 pKa = 8.92 FVCEE97 pKa = 4.15 SGKK100 pKa = 10.4 KK101 pKa = 9.78 FYY103 pKa = 11.14 SVTTNLLLRR112 pKa = 11.84 CDD114 pKa = 3.96 KK115 pKa = 10.93 ARR117 pKa = 11.84 SANFYY122 pKa = 10.46 FEE124 pKa = 4.41 WEE126 pKa = 4.04 VDD128 pKa = 3.78 FTTGFRR134 pKa = 11.84 AGWNFVMQYY143 pKa = 9.19 TLDD146 pKa = 5.07 LIASPYY152 pKa = 10.54 LNLSILPAIGYY163 pKa = 7.61 GTQQYY168 pKa = 8.85 QAILDD173 pKa = 4.11 GLHH176 pKa = 6.72 HH177 pKa = 7.11 EE178 pKa = 4.5 EE179 pKa = 4.94 AQDD182 pKa = 3.68 QAKK185 pKa = 8.55 STQPVISNLYY195 pKa = 8.0 PQLPTAPQVDD205 pKa = 3.7 TRR207 pKa = 11.84 MEE209 pKa = 4.55 ADD211 pKa = 3.74 LQQPPIKK218 pKa = 9.67 TKK220 pKa = 10.9 AKK222 pKa = 10.3 LLTRR226 pKa = 11.84 LFKK229 pKa = 10.83 RR230 pKa = 11.84 KK231 pKa = 9.2 ISKK234 pKa = 9.57 PP235 pKa = 3.42
Molecular weight: 26.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.746
IPC2_protein 6.898
IPC_protein 7.19
Toseland 6.927
ProMoST 7.761
Dawson 7.79
Bjellqvist 7.688
Wikipedia 7.732
Rodwell 7.761
Grimsley 6.956
Solomon 7.951
Lehninger 7.966
Nozaki 7.98
DTASelect 7.863
Thurlkill 7.907
EMBOSS 8.039
Sillero 8.17
Patrickios 4.393
IPC_peptide 7.951
IPC2_peptide 7.161
IPC2.peptide.svr19 7.453
Protein with the highest isoelectric point:
>tr|A0A1L3KHX9|A0A1L3KHX9_9VIRU Capsid protein OS=Sanxia permutotetra-like virus 1 OX=1923365 PE=3 SV=1
MM1 pKa = 7.86 PNNKK5 pKa = 9.22 NMNKK9 pKa = 10.07 RR10 pKa = 11.84 GQKK13 pKa = 9.39 PQAKK17 pKa = 9.07 KK18 pKa = 10.3 QKK20 pKa = 10.17 LPRR23 pKa = 11.84 KK24 pKa = 7.25 TVAGQPKK31 pKa = 9.94 AVNLLMGVPVTGRR44 pKa = 11.84 EE45 pKa = 3.81 VTAILTASANKK56 pKa = 9.88 TSVSYY61 pKa = 11.71 NNTFNIASASPMLKK75 pKa = 9.86 KK76 pKa = 10.39 YY77 pKa = 10.14 AAIYY81 pKa = 8.69 EE82 pKa = 4.37 SYY84 pKa = 10.24 RR85 pKa = 11.84 IKK87 pKa = 10.61 SVSYY91 pKa = 10.37 RR92 pKa = 11.84 FIPDD96 pKa = 3.16 EE97 pKa = 4.6 SGLSSGNISLGIDD110 pKa = 3.36 YY111 pKa = 10.94 GKK113 pKa = 10.1 MSGTLTRR120 pKa = 11.84 EE121 pKa = 3.96 QISRR125 pKa = 11.84 LNPHH129 pKa = 6.05 YY130 pKa = 10.5 SGPIRR135 pKa = 11.84 KK136 pKa = 7.79 ITPWVTIHH144 pKa = 6.64 PKK146 pKa = 9.97 FVNKK150 pKa = 9.27 DD151 pKa = 3.36 TVRR154 pKa = 11.84 YY155 pKa = 10.26 SGDD158 pKa = 3.71 DD159 pKa = 3.5 SLLSAPFTLALVATCEE175 pKa = 4.28 GKK177 pKa = 10.49 SVDD180 pKa = 3.65 RR181 pKa = 11.84 VLGCVEE187 pKa = 3.6 IQYY190 pKa = 9.03 TLEE193 pKa = 4.02 FQGILPP199 pKa = 4.34
Molecular weight: 21.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.127
IPC2_protein 9.37
IPC_protein 9.326
Toseland 10.014
ProMoST 9.692
Dawson 10.218
Bjellqvist 9.882
Wikipedia 10.379
Rodwell 10.701
Grimsley 10.277
Solomon 10.233
Lehninger 10.204
Nozaki 10.014
DTASelect 9.867
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.131
Patrickios 10.277
IPC_peptide 10.233
IPC2_peptide 8.478
IPC2.peptide.svr19 8.341
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1461
199
1027
487.0
54.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.666 ± 0.323
0.821 ± 0.176
5.818 ± 0.731
5.133 ± 0.991
3.217 ± 0.415
5.749 ± 0.339
1.574 ± 0.223
5.065 ± 0.606
7.255 ± 0.406
9.514 ± 0.477
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.468
3.696 ± 1.072
5.886 ± 0.294
4.997 ± 0.406
5.133 ± 0.432
6.639 ± 0.854
7.666 ± 0.36
6.845 ± 0.669
1.506 ± 0.262
3.149 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here