Sphingomonas ginsenosidivorax

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3625 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6UDW7|A0A5C6UDW7_9SPHN 30S ribosomal protein S2 OS=Sphingomonas ginsenosidivorax OX=862135 GN=rpsB PE=3 SV=1
MM1 pKa = 7.22KK2 pKa = 10.34VCVNADD8 pKa = 2.77ICAGFGLCIGIAPEE22 pKa = 4.1VFEE25 pKa = 5.13LHH27 pKa = 7.19DD28 pKa = 4.47DD29 pKa = 4.53GYY31 pKa = 10.99AVVMVSEE38 pKa = 4.27VQPEE42 pKa = 4.19DD43 pKa = 3.5EE44 pKa = 5.04DD45 pKa = 4.23MIRR48 pKa = 11.84SAARR52 pKa = 11.84QCPAQAITLGDD63 pKa = 4.17DD64 pKa = 3.59PTVSS68 pKa = 3.23

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6UJZ3|A0A5C6UJZ3_9SPHN Precorrin-2 dehydrogenase OS=Sphingomonas ginsenosidivorax OX=862135 GN=FSB78_16820 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIHH32 pKa = 6.17ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3625

0

3625

1204017

29

2699

332.1

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.855 ± 0.063

0.721 ± 0.012

6.236 ± 0.031

4.779 ± 0.042

3.446 ± 0.024

9.107 ± 0.045

1.907 ± 0.02

4.912 ± 0.025

2.722 ± 0.036

9.63 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.021

2.423 ± 0.031

5.371 ± 0.029

2.92 ± 0.021

7.428 ± 0.043

5.028 ± 0.03

5.954 ± 0.042

7.621 ± 0.032

1.406 ± 0.019

2.239 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski