Streptomyces virus phiBT1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lomovskayavirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q858Y4|Q858Y4_9CAUD Gp19 OS=Streptomyces virus phiBT1 OX=225588 GN=19 PE=4 SV=1
MM1 pKa = 7.45QYY3 pKa = 9.1GTASAEE9 pKa = 4.1ATQDD13 pKa = 3.09SAGAHH18 pKa = 6.17TYY20 pKa = 7.14TVRR23 pKa = 11.84ATFSDD28 pKa = 3.12QGYY31 pKa = 10.15SSTEE35 pKa = 3.88QLHH38 pKa = 5.68LTLDD42 pKa = 3.51AALEE46 pKa = 3.87LRR48 pKa = 11.84AALDD52 pKa = 3.63DD53 pKa = 3.95VLPP56 pKa = 4.58

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q858X6|Q858X6_9CAUD Gp25.1 OS=Streptomyces virus phiBT1 OX=225588 GN=25.1 PE=4 SV=1
MM1 pKa = 6.8MTPADD6 pKa = 4.04RR7 pKa = 11.84THH9 pKa = 5.24VRR11 pKa = 11.84RR12 pKa = 11.84MSLCSRR18 pKa = 11.84RR19 pKa = 11.84GLLSRR24 pKa = 11.84VTQRR28 pKa = 11.84PSSYY32 pKa = 10.07PLARR36 pKa = 11.84KK37 pKa = 9.82GDD39 pKa = 4.18FVSLPDD45 pKa = 3.17TDD47 pKa = 4.63ARR49 pKa = 11.84QTEE52 pKa = 4.48YY53 pKa = 10.0STYY56 pKa = 10.01APAGEE61 pKa = 3.94RR62 pKa = 11.84CRR64 pKa = 11.84RR65 pKa = 11.84CRR67 pKa = 11.84KK68 pKa = 9.02PFKK71 pKa = 10.68SLEE74 pKa = 3.97TCRR77 pKa = 11.84RR78 pKa = 11.84VANEE82 pKa = 4.06RR83 pKa = 11.84QSGTASADD91 pKa = 3.46RR92 pKa = 11.84YY93 pKa = 8.03EE94 pKa = 4.27HH95 pKa = 6.1VRR97 pKa = 11.84VLEE100 pKa = 3.98VSAPAKK106 pKa = 9.95RR107 pKa = 11.84LSARR111 pKa = 11.84VSALPEE117 pKa = 4.19PEE119 pKa = 4.86LVSLPMSPP127 pKa = 4.26

Molecular weight: 14.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12193

46

808

221.7

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.196 ± 0.384

1.017 ± 0.127

6.717 ± 0.281

5.995 ± 0.369

3.256 ± 0.143

8.694 ± 0.398

1.763 ± 0.146

3.559 ± 0.25

4.371 ± 0.382

7.726 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.097

3.035 ± 0.203

5.462 ± 0.31

2.428 ± 0.129

6.947 ± 0.511

5.593 ± 0.264

6.84 ± 0.387

7.8 ± 0.204

1.87 ± 0.154

2.633 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski