Amino acid dipepetide frequency for Streptomyces virus phiBT1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.338AlaAla: 15.338 ± 1.512
0.984AlaCys: 0.984 ± 0.321
9.432AlaAsp: 9.432 ± 0.856
7.956AlaGlu: 7.956 ± 1.01
3.281AlaPhe: 3.281 ± 0.383
8.448AlaGly: 8.448 ± 0.921
2.297AlaHis: 2.297 ± 0.448
4.429AlaIle: 4.429 ± 0.6
5.823AlaLys: 5.823 ± 0.818
10.417AlaLeu: 10.417 ± 0.837
3.117AlaMet: 3.117 ± 0.527
3.035AlaAsn: 3.035 ± 0.618
6.07AlaPro: 6.07 ± 0.823
4.265AlaGln: 4.265 ± 0.467
7.792AlaArg: 7.792 ± 1.065
5.413AlaSer: 5.413 ± 0.661
8.284AlaThr: 8.284 ± 0.886
9.268AlaVal: 9.268 ± 1.033
2.461AlaTrp: 2.461 ± 0.474
3.773AlaTyr: 3.773 ± 0.706
0.082AlaXaa: 0.082 ± 0.072
Cys
1.148CysAla: 1.148 ± 0.373
0.0CysCys: 0.0 ± 0.0
0.82CysAsp: 0.82 ± 0.264
0.574CysGlu: 0.574 ± 0.202
0.164CysPhe: 0.164 ± 0.099
0.902CysGly: 0.902 ± 0.245
0.164CysHis: 0.164 ± 0.109
0.246CysIle: 0.246 ± 0.154
0.328CysLys: 0.328 ± 0.141
0.41CysLeu: 0.41 ± 0.2
0.246CysMet: 0.246 ± 0.136
0.41CysAsn: 0.41 ± 0.205
0.656CysPro: 0.656 ± 0.231
0.328CysGln: 0.328 ± 0.163
1.066CysArg: 1.066 ± 0.356
0.574CysSer: 0.574 ± 0.225
0.902CysThr: 0.902 ± 0.308
0.82CysVal: 0.82 ± 0.207
0.164CysTrp: 0.164 ± 0.115
0.246CysTyr: 0.246 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
10.171AspAla: 10.171 ± 1.048
0.574AspCys: 0.574 ± 0.246
5.003AspAsp: 5.003 ± 1.088
4.265AspGlu: 4.265 ± 0.749
2.133AspPhe: 2.133 ± 0.342
5.988AspGly: 5.988 ± 0.612
1.394AspHis: 1.394 ± 0.304
1.23AspIle: 1.23 ± 0.36
1.722AspLys: 1.722 ± 0.352
5.741AspLeu: 5.741 ± 0.662
1.64AspMet: 1.64 ± 0.33
1.64AspAsn: 1.64 ± 0.477
4.429AspPro: 4.429 ± 0.52
0.656AspGln: 0.656 ± 0.206
3.527AspArg: 3.527 ± 0.651
2.707AspSer: 2.707 ± 0.596
4.101AspThr: 4.101 ± 0.451
6.808AspVal: 6.808 ± 0.718
1.886AspTrp: 1.886 ± 0.411
1.558AspTyr: 1.558 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
7.3GluAla: 7.3 ± 0.722
0.574GluCys: 0.574 ± 0.209
2.625GluAsp: 2.625 ± 0.516
3.281GluGlu: 3.281 ± 0.527
2.789GluPhe: 2.789 ± 0.411
5.413GluGly: 5.413 ± 0.643
1.312GluHis: 1.312 ± 0.358
2.707GluIle: 2.707 ± 0.537
2.133GluLys: 2.133 ± 0.654
5.906GluLeu: 5.906 ± 1.084
0.984GluMet: 0.984 ± 0.248
1.148GluAsn: 1.148 ± 0.345
3.527GluPro: 3.527 ± 0.77
0.902GluGln: 0.902 ± 0.225
5.906GluArg: 5.906 ± 0.808
2.953GluSer: 2.953 ± 0.466
4.101GluThr: 4.101 ± 0.531
3.855GluVal: 3.855 ± 0.529
0.902GluTrp: 0.902 ± 0.265
1.64GluTyr: 1.64 ± 0.401
0.0GluXaa: 0.0 ± 0.0
Phe
4.429PheAla: 4.429 ± 0.602
0.164PheCys: 0.164 ± 0.113
2.215PheAsp: 2.215 ± 0.535
1.886PheGlu: 1.886 ± 0.537
0.492PhePhe: 0.492 ± 0.19
2.707PheGly: 2.707 ± 0.464
0.246PheHis: 0.246 ± 0.132
1.23PheIle: 1.23 ± 0.321
1.64PheLys: 1.64 ± 0.318
3.035PheLeu: 3.035 ± 0.447
0.738PheMet: 0.738 ± 0.321
1.148PheAsn: 1.148 ± 0.296
1.886PhePro: 1.886 ± 0.437
0.82PheGln: 0.82 ± 0.227
2.625PheArg: 2.625 ± 0.529
1.558PheSer: 1.558 ± 0.317
2.707PheThr: 2.707 ± 0.433
1.969PheVal: 1.969 ± 0.409
0.246PheTrp: 0.246 ± 0.146
0.738PheTyr: 0.738 ± 0.262
0.0PheXaa: 0.0 ± 0.0
Gly
11.073GlyAla: 11.073 ± 1.031
1.066GlyCys: 1.066 ± 0.259
4.757GlyAsp: 4.757 ± 0.666
5.331GlyGlu: 5.331 ± 0.718
3.445GlyPhe: 3.445 ± 0.555
6.316GlyGly: 6.316 ± 0.856
1.148GlyHis: 1.148 ± 0.292
4.019GlyIle: 4.019 ± 0.644
4.511GlyLys: 4.511 ± 0.602
6.972GlyLeu: 6.972 ± 1.061
1.64GlyMet: 1.64 ± 0.368
2.707GlyAsn: 2.707 ± 0.49
4.675GlyPro: 4.675 ± 1.095
2.215GlyGln: 2.215 ± 0.376
6.316GlyArg: 6.316 ± 0.569
4.757GlySer: 4.757 ± 0.775
5.167GlyThr: 5.167 ± 0.986
5.331GlyVal: 5.331 ± 0.735
2.379GlyTrp: 2.379 ± 0.464
3.117GlyTyr: 3.117 ± 0.592
0.0GlyXaa: 0.0 ± 0.0
His
1.722HisAla: 1.722 ± 0.425
0.164HisCys: 0.164 ± 0.107
1.64HisAsp: 1.64 ± 0.324
1.066HisGlu: 1.066 ± 0.387
0.492HisPhe: 0.492 ± 0.188
1.64HisGly: 1.64 ± 0.506
0.328HisHis: 0.328 ± 0.16
0.984HisIle: 0.984 ± 0.242
0.574HisLys: 0.574 ± 0.214
1.23HisLeu: 1.23 ± 0.326
0.328HisMet: 0.328 ± 0.152
0.574HisAsn: 0.574 ± 0.221
1.394HisPro: 1.394 ± 0.382
0.082HisGln: 0.082 ± 0.082
0.738HisArg: 0.738 ± 0.215
1.394HisSer: 1.394 ± 0.297
1.148HisThr: 1.148 ± 0.317
1.394HisVal: 1.394 ± 0.33
0.574HisTrp: 0.574 ± 0.241
0.164HisTyr: 0.164 ± 0.111
0.0HisXaa: 0.0 ± 0.0
Ile
4.019IleAla: 4.019 ± 0.533
0.246IleCys: 0.246 ± 0.128
3.937IleAsp: 3.937 ± 0.602
1.722IleGlu: 1.722 ± 0.338
0.902IlePhe: 0.902 ± 0.25
3.199IleGly: 3.199 ± 0.507
0.984IleHis: 0.984 ± 0.394
1.394IleIle: 1.394 ± 0.32
1.886IleLys: 1.886 ± 0.357
2.215IleLeu: 2.215 ± 0.392
0.82IleMet: 0.82 ± 0.21
0.902IleAsn: 0.902 ± 0.3
2.215IlePro: 2.215 ± 0.339
0.656IleGln: 0.656 ± 0.207
2.625IleArg: 2.625 ± 0.502
1.722IleSer: 1.722 ± 0.255
2.625IleThr: 2.625 ± 0.475
2.297IleVal: 2.297 ± 0.504
0.41IleTrp: 0.41 ± 0.146
0.82IleTyr: 0.82 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
5.659LysAla: 5.659 ± 0.817
0.492LysCys: 0.492 ± 0.204
2.707LysAsp: 2.707 ± 0.43
2.051LysGlu: 2.051 ± 0.468
1.312LysPhe: 1.312 ± 0.32
3.937LysGly: 3.937 ± 0.67
1.066LysHis: 1.066 ± 0.245
1.148LysIle: 1.148 ± 0.362
1.969LysLys: 1.969 ± 0.412
3.117LysLeu: 3.117 ± 0.589
0.902LysMet: 0.902 ± 0.25
0.902LysAsn: 0.902 ± 0.285
2.953LysPro: 2.953 ± 0.575
0.82LysGln: 0.82 ± 0.235
3.445LysArg: 3.445 ± 0.481
2.543LysSer: 2.543 ± 0.563
2.789LysThr: 2.789 ± 0.572
3.691LysVal: 3.691 ± 0.456
0.82LysTrp: 0.82 ± 0.266
1.394LysTyr: 1.394 ± 0.392
0.0LysXaa: 0.0 ± 0.0
Leu
7.3LeuAla: 7.3 ± 0.835
0.574LeuCys: 0.574 ± 0.223
7.546LeuAsp: 7.546 ± 0.849
3.937LeuGlu: 3.937 ± 0.578
2.379LeuPhe: 2.379 ± 0.413
6.152LeuGly: 6.152 ± 0.579
0.984LeuHis: 0.984 ± 0.292
3.773LeuIle: 3.773 ± 0.59
4.593LeuLys: 4.593 ± 0.69
4.511LeuLeu: 4.511 ± 0.635
0.984LeuMet: 0.984 ± 0.234
2.707LeuAsn: 2.707 ± 0.458
5.167LeuPro: 5.167 ± 0.644
2.215LeuGln: 2.215 ± 0.46
5.906LeuArg: 5.906 ± 1.069
5.988LeuSer: 5.988 ± 0.616
5.085LeuThr: 5.085 ± 0.689
5.085LeuVal: 5.085 ± 0.555
0.984LeuTrp: 0.984 ± 0.287
1.394LeuTyr: 1.394 ± 0.338
0.0LeuXaa: 0.0 ± 0.0
Met
2.953MetAla: 2.953 ± 0.475
0.328MetCys: 0.328 ± 0.123
0.82MetAsp: 0.82 ± 0.227
0.328MetGlu: 0.328 ± 0.148
0.246MetPhe: 0.246 ± 0.132
1.969MetGly: 1.969 ± 0.455
0.492MetHis: 0.492 ± 0.208
0.574MetIle: 0.574 ± 0.264
0.984MetLys: 0.984 ± 0.234
1.722MetLeu: 1.722 ± 0.307
0.574MetMet: 0.574 ± 0.207
0.656MetAsn: 0.656 ± 0.223
1.394MetPro: 1.394 ± 0.356
0.574MetGln: 0.574 ± 0.192
1.64MetArg: 1.64 ± 0.395
1.886MetSer: 1.886 ± 0.366
1.558MetThr: 1.558 ± 0.356
1.312MetVal: 1.312 ± 0.381
0.574MetTrp: 0.574 ± 0.196
0.328MetTyr: 0.328 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
4.839AsnAla: 4.839 ± 0.565
0.328AsnCys: 0.328 ± 0.152
1.476AsnAsp: 1.476 ± 0.364
1.804AsnGlu: 1.804 ± 0.344
0.902AsnPhe: 0.902 ± 0.251
3.855AsnGly: 3.855 ± 0.461
0.656AsnHis: 0.656 ± 0.171
0.984AsnIle: 0.984 ± 0.378
1.23AsnLys: 1.23 ± 0.311
2.133AsnLeu: 2.133 ± 0.376
0.492AsnMet: 0.492 ± 0.177
1.148AsnAsn: 1.148 ± 0.305
2.707AsnPro: 2.707 ± 0.423
0.656AsnGln: 0.656 ± 0.301
0.984AsnArg: 0.984 ± 0.255
1.148AsnSer: 1.148 ± 0.356
1.23AsnThr: 1.23 ± 0.275
2.953AsnVal: 2.953 ± 0.46
0.41AsnTrp: 0.41 ± 0.209
0.41AsnTyr: 0.41 ± 0.159
0.0AsnXaa: 0.0 ± 0.0
Pro
7.054ProAla: 7.054 ± 0.957
0.574ProCys: 0.574 ± 0.24
4.757ProAsp: 4.757 ± 0.712
4.921ProGlu: 4.921 ± 0.725
2.461ProPhe: 2.461 ± 0.383
5.906ProGly: 5.906 ± 0.813
0.82ProHis: 0.82 ± 0.217
1.394ProIle: 1.394 ± 0.336
3.035ProLys: 3.035 ± 0.584
3.199ProLeu: 3.199 ± 0.486
1.312ProMet: 1.312 ± 0.347
2.133ProAsn: 2.133 ± 0.399
0.574ProPro: 0.574 ± 0.294
0.902ProGln: 0.902 ± 0.4
1.804ProArg: 1.804 ± 0.342
3.855ProSer: 3.855 ± 0.484
4.265ProThr: 4.265 ± 0.571
4.101ProVal: 4.101 ± 0.663
0.656ProTrp: 0.656 ± 0.228
0.902ProTyr: 0.902 ± 0.223
0.0ProXaa: 0.0 ± 0.0
Gln
2.871GlnAla: 2.871 ± 0.533
0.246GlnCys: 0.246 ± 0.13
0.984GlnAsp: 0.984 ± 0.218
0.902GlnGlu: 0.902 ± 0.283
0.82GlnPhe: 0.82 ± 0.215
2.707GlnGly: 2.707 ± 0.472
0.656GlnHis: 0.656 ± 0.256
0.738GlnIle: 0.738 ± 0.298
0.41GlnLys: 0.41 ± 0.177
1.476GlnLeu: 1.476 ± 0.307
0.738GlnMet: 0.738 ± 0.243
1.066GlnAsn: 1.066 ± 0.339
1.476GlnPro: 1.476 ± 0.342
0.41GlnGln: 0.41 ± 0.162
2.953GlnArg: 2.953 ± 0.578
1.23GlnSer: 1.23 ± 0.333
1.722GlnThr: 1.722 ± 0.369
1.64GlnVal: 1.64 ± 0.329
0.41GlnTrp: 0.41 ± 0.207
0.82GlnTyr: 0.82 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
8.694ArgAla: 8.694 ± 1.151
0.902ArgCys: 0.902 ± 0.323
3.527ArgAsp: 3.527 ± 0.693
4.511ArgGlu: 4.511 ± 0.723
3.281ArgPhe: 3.281 ± 0.474
4.757ArgGly: 4.757 ± 0.499
1.722ArgHis: 1.722 ± 0.388
2.707ArgIle: 2.707 ± 0.506
3.855ArgLys: 3.855 ± 0.527
5.167ArgLeu: 5.167 ± 0.62
1.804ArgMet: 1.804 ± 0.421
2.543ArgAsn: 2.543 ± 0.469
2.051ArgPro: 2.051 ± 0.423
1.394ArgGln: 1.394 ± 0.305
5.167ArgArg: 5.167 ± 1.018
4.429ArgSer: 4.429 ± 0.706
3.691ArgThr: 3.691 ± 0.573
5.823ArgVal: 5.823 ± 0.748
1.64ArgTrp: 1.64 ± 0.44
1.804ArgTyr: 1.804 ± 0.546
0.0ArgXaa: 0.0 ± 0.0
Ser
7.054SerAla: 7.054 ± 1.073
0.574SerCys: 0.574 ± 0.291
3.527SerAsp: 3.527 ± 0.537
3.609SerGlu: 3.609 ± 0.479
1.886SerPhe: 1.886 ± 0.396
5.741SerGly: 5.741 ± 0.923
0.902SerHis: 0.902 ± 0.277
1.969SerIle: 1.969 ± 0.319
2.215SerLys: 2.215 ± 0.389
4.593SerLeu: 4.593 ± 0.686
0.902SerMet: 0.902 ± 0.212
1.558SerAsn: 1.558 ± 0.435
2.707SerPro: 2.707 ± 0.521
1.394SerGln: 1.394 ± 0.257
2.543SerArg: 2.543 ± 0.374
3.117SerSer: 3.117 ± 0.583
4.675SerThr: 4.675 ± 0.685
4.593SerVal: 4.593 ± 0.569
0.984SerTrp: 0.984 ± 0.295
1.394SerTyr: 1.394 ± 0.351
0.0SerXaa: 0.0 ± 0.0
Thr
6.398ThrAla: 6.398 ± 0.502
0.738ThrCys: 0.738 ± 0.225
4.429ThrAsp: 4.429 ± 0.735
3.773ThrGlu: 3.773 ± 0.503
2.215ThrPhe: 2.215 ± 0.394
6.808ThrGly: 6.808 ± 0.946
0.738ThrHis: 0.738 ± 0.231
2.133ThrIle: 2.133 ± 0.526
2.625ThrLys: 2.625 ± 0.422
6.48ThrLeu: 6.48 ± 0.815
0.984ThrMet: 0.984 ± 0.256
2.543ThrAsn: 2.543 ± 0.382
4.183ThrPro: 4.183 ± 0.852
2.379ThrGln: 2.379 ± 0.564
3.527ThrArg: 3.527 ± 0.694
3.199ThrSer: 3.199 ± 0.472
5.249ThrThr: 5.249 ± 0.806
6.644ThrVal: 6.644 ± 0.94
1.066ThrTrp: 1.066 ± 0.335
2.297ThrTyr: 2.297 ± 0.487
0.0ThrXaa: 0.0 ± 0.0
Val
7.464ValAla: 7.464 ± 0.814
0.984ValCys: 0.984 ± 0.298
3.773ValAsp: 3.773 ± 0.464
4.839ValGlu: 4.839 ± 0.631
1.969ValPhe: 1.969 ± 0.42
5.988ValGly: 5.988 ± 0.789
1.312ValHis: 1.312 ± 0.324
2.953ValIle: 2.953 ± 0.411
3.035ValLys: 3.035 ± 0.66
6.316ValLeu: 6.316 ± 0.582
1.64ValMet: 1.64 ± 0.377
2.379ValAsn: 2.379 ± 0.41
4.675ValPro: 4.675 ± 0.547
2.133ValGln: 2.133 ± 0.369
6.808ValArg: 6.808 ± 0.612
5.249ValSer: 5.249 ± 0.703
5.988ValThr: 5.988 ± 0.718
6.07ValVal: 6.07 ± 0.636
1.804ValTrp: 1.804 ± 0.372
2.625ValTyr: 2.625 ± 0.323
0.0ValXaa: 0.0 ± 0.0
Trp
2.707TrpAla: 2.707 ± 0.589
0.246TrpCys: 0.246 ± 0.143
1.476TrpAsp: 1.476 ± 0.367
0.902TrpGlu: 0.902 ± 0.314
0.984TrpPhe: 0.984 ± 0.325
1.23TrpGly: 1.23 ± 0.293
0.164TrpHis: 0.164 ± 0.122
0.574TrpIle: 0.574 ± 0.214
0.492TrpLys: 0.492 ± 0.185
1.722TrpLeu: 1.722 ± 0.367
0.082TrpMet: 0.082 ± 0.075
0.328TrpAsn: 0.328 ± 0.145
0.328TrpPro: 0.328 ± 0.205
0.902TrpGln: 0.902 ± 0.228
1.804TrpArg: 1.804 ± 0.448
1.066TrpSer: 1.066 ± 0.263
1.394TrpThr: 1.394 ± 0.303
1.722TrpVal: 1.722 ± 0.373
0.082TrpTrp: 0.082 ± 0.088
0.492TrpTyr: 0.492 ± 0.263
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.773TyrAla: 3.773 ± 0.578
0.41TyrCys: 0.41 ± 0.151
1.722TyrAsp: 1.722 ± 0.286
2.297TyrGlu: 2.297 ± 0.471
0.41TyrPhe: 0.41 ± 0.205
3.281TyrGly: 3.281 ± 0.516
0.246TyrHis: 0.246 ± 0.16
0.328TyrIle: 0.328 ± 0.15
0.656TyrLys: 0.656 ± 0.237
0.902TyrLeu: 0.902 ± 0.268
0.902TyrMet: 0.902 ± 0.255
0.82TyrAsn: 0.82 ± 0.234
1.558TyrPro: 1.558 ± 0.465
0.574TyrGln: 0.574 ± 0.207
2.297TyrArg: 2.297 ± 0.602
1.148TyrSer: 1.148 ± 0.348
1.722TyrThr: 1.722 ± 0.362
2.625TyrVal: 2.625 ± 0.495
0.246TyrTrp: 0.246 ± 0.127
0.41TyrTyr: 0.41 ± 0.178
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.082XaaIle: 0.082 ± 0.072
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12193 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski