Arthrobacter sp. SW1
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3661 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E8FNB8|A0A1E8FNB8_9MICC Transcriptional regulator OS=Arthrobacter sp. SW1 OX=1920889 GN=BIU82_10275 PE=4 SV=1
MM1 pKa = 7.2 VFLAALSTLALSTFGLTGASADD23 pKa = 3.71 PDD25 pKa = 4.01 GQHH28 pKa = 6.87 GSDD31 pKa = 3.56 EE32 pKa = 4.14 GHH34 pKa = 7.42 LIGTGATGKK43 pKa = 9.57 IQLLDD48 pKa = 3.71 VVDD51 pKa = 4.11 VTDD54 pKa = 4.19 TPDD57 pKa = 4.39 LIADD61 pKa = 3.8 VTVSPDD67 pKa = 2.82 GNTAFLANWGEE78 pKa = 4.02 PDD80 pKa = 3.55 CAGPEE85 pKa = 4.08 TGGQNSPDD93 pKa = 3.18 AGAYY97 pKa = 10.18 VIDD100 pKa = 3.7 ITNIEE105 pKa = 4.36 TDD107 pKa = 3.6 PEE109 pKa = 4.31 SAKK112 pKa = 10.92 LIGFIPSHH120 pKa = 6.27 QDD122 pKa = 2.95 TRR124 pKa = 11.84 PGEE127 pKa = 4.14 GMQVVNVTTAQFSGDD142 pKa = 3.2 ILVMNNEE149 pKa = 4.18 ACGKK153 pKa = 7.78 NAKK156 pKa = 10.17 GGVSLWDD163 pKa = 3.32 VTDD166 pKa = 3.71 PANPKK171 pKa = 9.96 KK172 pKa = 10.6 LSEE175 pKa = 4.26 HH176 pKa = 6.22 FGDD179 pKa = 4.36 RR180 pKa = 11.84 EE181 pKa = 4.03 PGTRR185 pKa = 11.84 DD186 pKa = 2.99 ANEE189 pKa = 3.72 IHH191 pKa = 7.12 SAFAWDD197 pKa = 4.07 AGNNAYY203 pKa = 10.72 LVMTDD208 pKa = 3.75 NEE210 pKa = 4.25 QFPDD214 pKa = 3.64 VDD216 pKa = 4.4 ILDD219 pKa = 3.53 ITNPHH224 pKa = 6.78 RR225 pKa = 11.84 PRR227 pKa = 11.84 LIAEE231 pKa = 4.03 YY232 pKa = 10.22 NLNDD236 pKa = 3.83 FAVDD240 pKa = 3.74 QPEE243 pKa = 4.22 LGLTEE248 pKa = 4.92 SFLHH252 pKa = 6.65 DD253 pKa = 3.67 MVVKK257 pKa = 10.54 EE258 pKa = 3.6 IDD260 pKa = 3.19 GRR262 pKa = 11.84 FIMLLSYY269 pKa = 10.21 WDD271 pKa = 3.57 GGYY274 pKa = 10.86 VLLDD278 pKa = 3.27 VTDD281 pKa = 3.92 PANAVFIGDD290 pKa = 3.62 TDD292 pKa = 4.29 FAAVDD297 pKa = 3.67 PEE299 pKa = 4.37 LFEE302 pKa = 4.53 SLGVALTPEE311 pKa = 4.76 GNGHH315 pKa = 5.0 QAEE318 pKa = 4.36 FTKK321 pKa = 11.06 DD322 pKa = 2.91 NAFFIGTDD330 pKa = 3.29 EE331 pKa = 5.03 DD332 pKa = 3.87 FAPYY336 pKa = 8.79 RR337 pKa = 11.84 TDD339 pKa = 3.07 ALRR342 pKa = 11.84 ITSGAFQGEE351 pKa = 4.68 YY352 pKa = 10.45 EE353 pKa = 4.41 SVIVPGGQPPAVLEE367 pKa = 4.4 DD368 pKa = 3.84 LTLSGPVVYY377 pKa = 10.22 GGYY380 pKa = 10.05 GCPASAPVPSPEE392 pKa = 4.37 DD393 pKa = 3.33 VPGYY397 pKa = 9.76 VASLQPGDD405 pKa = 3.64 EE406 pKa = 4.63 TILVLQRR413 pKa = 11.84 GPSGDD418 pKa = 3.53 PSATEE423 pKa = 3.87 EE424 pKa = 4.07 ACFPGEE430 pKa = 4.17 KK431 pKa = 9.73 AHH433 pKa = 6.42 EE434 pKa = 4.21 AALAGWDD441 pKa = 3.03 AVLFVQRR448 pKa = 11.84 HH449 pKa = 4.77 LPAGEE454 pKa = 4.41 EE455 pKa = 3.97 DD456 pKa = 3.73 TAFCGSGAFVDD467 pKa = 5.16 SIVAVCTTHH476 pKa = 6.86 EE477 pKa = 4.26 AFHH480 pKa = 6.76 KK481 pKa = 10.56 LFNLEE486 pKa = 4.38 PLDD489 pKa = 4.12 PAQWSYY495 pKa = 11.63 PEE497 pKa = 4.13 NLAIGAIGDD506 pKa = 4.43 RR507 pKa = 11.84 IRR509 pKa = 11.84 VGSIFDD515 pKa = 3.07 GWGYY519 pKa = 10.71 VHH521 pKa = 7.34 LFDD524 pKa = 4.95 TATLQEE530 pKa = 4.75 LDD532 pKa = 3.47 TYY534 pKa = 10.83 AIPEE538 pKa = 3.94 AHH540 pKa = 7.03 DD541 pKa = 3.2 PAFAFGYY548 pKa = 10.52 GDD550 pKa = 3.97 LSVHH554 pKa = 5.78 EE555 pKa = 4.57 VAVDD559 pKa = 3.68 PQDD562 pKa = 3.59 PSLAYY567 pKa = 10.33 LSYY570 pKa = 11.08 YY571 pKa = 10.66 SGGLRR576 pKa = 11.84 AIQIQCGGVPYY587 pKa = 10.76 DD588 pKa = 4.55 ADD590 pKa = 3.93 NPPADD595 pKa = 4.07 KK596 pKa = 10.85 GSCEE600 pKa = 3.93 LVEE603 pKa = 4.28 VGSYY607 pKa = 10.9 LDD609 pKa = 3.49 PAGNDD614 pKa = 3.07 FWGVEE619 pKa = 4.16 TFIGDD624 pKa = 3.83 DD625 pKa = 3.15 GRR627 pKa = 11.84 TYY629 pKa = 11.0 ILGSDD634 pKa = 3.72 RR635 pKa = 11.84 DD636 pKa = 3.64 SGLWIFTDD644 pKa = 3.86 PP645 pKa = 4.44
Molecular weight: 68.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.834
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.986
Patrickios 1.532
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A1E8FVW3|A0A1E8FVW3_9MICC AB hydrolase-1 domain-containing protein OS=Arthrobacter sp. SW1 OX=1920889 GN=BIU82_02175 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.61 KK12 pKa = 9.72 IPGAQVVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GKK24 pKa = 8.59 TFVINKK30 pKa = 8.84 KK31 pKa = 9.15 NPRR34 pKa = 11.84 FKK36 pKa = 10.85 ARR38 pKa = 11.84 QGG40 pKa = 3.28
Molecular weight: 4.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.052
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.618
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.34
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.054
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3661
0
3661
1202471
37
2047
328.5
35.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.352 ± 0.059
0.625 ± 0.009
5.444 ± 0.036
5.87 ± 0.046
3.334 ± 0.03
9.295 ± 0.038
2.009 ± 0.019
4.279 ± 0.033
2.965 ± 0.033
10.381 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.018
2.428 ± 0.024
5.438 ± 0.033
2.96 ± 0.022
6.409 ± 0.042
5.846 ± 0.025
5.55 ± 0.033
8.331 ± 0.037
1.415 ± 0.018
2.089 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here