Candidatus Synechococcus spongiarum LMB bulk10E

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; Candidatus Synechococcus spongiarum

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 427 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N4ARF1|A0A6N4ARF1_9SYNE Uncharacterized protein OS=Candidatus Synechococcus spongiarum LMB bulk10E OX=1449122 GN=BO91_00140 PE=4 SV=1
MM1 pKa = 6.84YY2 pKa = 10.22QNKK5 pKa = 9.65SLSHH9 pKa = 6.81NYY11 pKa = 8.18TAITNHH17 pKa = 6.29CLDD20 pKa = 3.02TGMYY24 pKa = 8.87FGCIKK29 pKa = 10.33GFPGAHH35 pKa = 5.56SQGEE39 pKa = 4.41TLDD42 pKa = 3.77EE43 pKa = 4.98LNTNLQEE50 pKa = 4.44VISMLNDD57 pKa = 3.33DD58 pKa = 4.77NVPVFIDD65 pKa = 3.13NNEE68 pKa = 4.01YY69 pKa = 10.7VDD71 pKa = 3.8RR72 pKa = 11.84QTFISII78 pKa = 3.85

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N4APX0|A0A6N4APX0_9SYNE Uncharacterized protein OS=Candidatus Synechococcus spongiarum LMB bulk10E OX=1449122 GN=BO91_02025 PE=4 SV=1
MM1 pKa = 7.65ALLNGSPLGQRR12 pKa = 11.84PTGIGVVTLALAKK25 pKa = 9.97ALCHH29 pKa = 5.83EE30 pKa = 4.78RR31 pKa = 11.84VTFLDD36 pKa = 3.91PVATGRR42 pKa = 11.84PNSLAIPGNLSMDD55 pKa = 3.94HH56 pKa = 5.92GRR58 pKa = 11.84KK59 pKa = 8.71GHH61 pKa = 6.01LRR63 pKa = 11.84RR64 pKa = 11.84LLWTQNQLPGLLKK77 pKa = 9.96KK78 pKa = 9.26TGTSLLLSPLPEE90 pKa = 5.54APMLRR95 pKa = 11.84RR96 pKa = 11.84GVRR99 pKa = 11.84SIVLAHH105 pKa = 6.74DD106 pKa = 5.56LIPLRR111 pKa = 11.84FPRR114 pKa = 11.84PGPLLAYY121 pKa = 9.75HH122 pKa = 7.06LAYY125 pKa = 10.52VPLVLHH131 pKa = 4.88QAEE134 pKa = 3.87RR135 pKa = 11.84VLCNSEE141 pKa = 3.43ATARR145 pKa = 11.84EE146 pKa = 3.58IHH148 pKa = 5.93HH149 pKa = 6.73WLRR152 pKa = 11.84VPLRR156 pKa = 11.84KK157 pKa = 9.97LITVPLGFDD166 pKa = 3.64PGQLRR171 pKa = 11.84PLDD174 pKa = 3.86VPRR177 pKa = 11.84QPFFLVLGRR186 pKa = 11.84HH187 pKa = 6.66DD188 pKa = 3.63YY189 pKa = 10.92HH190 pKa = 7.67KK191 pKa = 10.7NLPRR195 pKa = 11.84ILRR198 pKa = 11.84AFARR202 pKa = 11.84IADD205 pKa = 3.84PDD207 pKa = 3.74MTLKK211 pKa = 11.01LVGSPHH217 pKa = 7.22AYY219 pKa = 8.44LTAKK223 pKa = 10.2LKK225 pKa = 10.75HH226 pKa = 5.35LAARR230 pKa = 11.84LRR232 pKa = 11.84IAHH235 pKa = 4.98QCQWIHH241 pKa = 5.15WVSDD245 pKa = 3.48AEE247 pKa = 4.45RR248 pKa = 11.84LRR250 pKa = 11.84LLNTAKK256 pKa = 10.77ALVMPSLWEE265 pKa = 4.25GFGLPALEE273 pKa = 5.22AMACGTPVIAGNAGALSEE291 pKa = 4.48VVGSVGLLVNPTNTSHH307 pKa = 7.18IAEE310 pKa = 4.39AMADD314 pKa = 3.55VLSDD318 pKa = 3.94NKK320 pKa = 10.23IQRR323 pKa = 11.84QARR326 pKa = 11.84LGGPSRR332 pKa = 11.84AARR335 pKa = 11.84FRR337 pKa = 11.84WDD339 pKa = 2.88NTARR343 pKa = 11.84QVEE346 pKa = 4.46SLLEE350 pKa = 4.04NFF352 pKa = 4.8

Molecular weight: 38.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

427

0

427

116829

17

2453

273.6

30.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.147 ± 0.172

1.399 ± 0.048

5.094 ± 0.089

5.452 ± 0.131

3.117 ± 0.079

8.232 ± 0.114

2.54 ± 0.079

3.955 ± 0.102

2.747 ± 0.125

11.964 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.054

2.628 ± 0.074

5.791 ± 0.102

4.779 ± 0.091

7.663 ± 0.119

5.903 ± 0.109

5.387 ± 0.125

7.451 ± 0.108

1.631 ± 0.055

2.022 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski