Ralstonia phage RpY1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7JF02|A0A7D7JF02_9CAUD Structural protein OS=Ralstonia phage RpY1 OX=2759121 GN=RpY1_046 PE=4 SV=1
MM1 pKa = 7.36ATTAQILAFIKK12 pKa = 10.25EE13 pKa = 4.18HH14 pKa = 6.7GFVCSDD20 pKa = 3.61NNDD23 pKa = 2.91GTLRR27 pKa = 11.84VVDD30 pKa = 4.4VYY32 pKa = 11.73YY33 pKa = 10.81EE34 pKa = 4.33DD35 pKa = 3.69GNAYY39 pKa = 9.97EE40 pKa = 4.79EE41 pKa = 4.8IEE43 pKa = 4.89HH44 pKa = 6.86IPATVKK50 pKa = 10.52AARR53 pKa = 11.84DD54 pKa = 3.5WLGYY58 pKa = 10.28

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7ITC4|A0A7D7ITC4_9CAUD Uncharacterized protein OS=Ralstonia phage RpY1 OX=2759121 GN=RpY1_037 PE=4 SV=1
MM1 pKa = 7.45QIQIKK6 pKa = 10.36HH7 pKa = 6.17RR8 pKa = 11.84FTGAASFPLSNNAPARR24 pKa = 11.84VGRR27 pKa = 11.84LRR29 pKa = 11.84AYY31 pKa = 10.44GNAINAIQAQIWCEE45 pKa = 3.74TVMAALSIRR54 pKa = 11.84AAAAIGDD61 pKa = 4.23SNEE64 pKa = 3.73

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12409

41

1069

234.1

25.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.886 ± 0.471

0.854 ± 0.154

5.762 ± 0.244

5.149 ± 0.526

3.086 ± 0.22

8.542 ± 0.451

1.684 ± 0.163

4.368 ± 0.281

4.215 ± 0.341

8.026 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.189

4.086 ± 0.363

4.747 ± 0.265

4.843 ± 0.405

6.165 ± 0.422

5.528 ± 0.345

6.278 ± 0.494

6.665 ± 0.345

1.692 ± 0.165

2.796 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski