Amino acid dipepetide frequency for Ralstonia phage RpY1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.02AlaAla: 19.02 ± 2.189
1.128AlaCys: 1.128 ± 0.329
7.737AlaAsp: 7.737 ± 1.268
6.044AlaGlu: 6.044 ± 1.06
3.788AlaPhe: 3.788 ± 0.508
9.752AlaGly: 9.752 ± 0.97
2.257AlaHis: 2.257 ± 0.429
5.803AlaIle: 5.803 ± 0.769
6.044AlaLys: 6.044 ± 0.711
9.752AlaLeu: 9.752 ± 0.961
3.304AlaMet: 3.304 ± 0.685
5.803AlaAsn: 5.803 ± 0.697
6.931AlaPro: 6.931 ± 0.85
6.447AlaGln: 6.447 ± 0.841
6.125AlaArg: 6.125 ± 0.762
6.528AlaSer: 6.528 ± 0.618
8.382AlaThr: 8.382 ± 0.999
8.543AlaVal: 8.543 ± 0.767
1.209AlaTrp: 1.209 ± 0.307
4.271AlaTyr: 4.271 ± 0.49
0.0AlaXaa: 0.0 ± 0.0
Cys
0.725CysAla: 0.725 ± 0.217
0.0CysCys: 0.0 ± 0.0
0.564CysAsp: 0.564 ± 0.186
0.403CysGlu: 0.403 ± 0.159
0.484CysPhe: 0.484 ± 0.154
0.403CysGly: 0.403 ± 0.222
0.242CysHis: 0.242 ± 0.146
0.322CysIle: 0.322 ± 0.16
0.242CysLys: 0.242 ± 0.117
0.967CysLeu: 0.967 ± 0.272
0.0CysMet: 0.0 ± 0.0
0.484CysAsn: 0.484 ± 0.221
0.564CysPro: 0.564 ± 0.312
0.322CysGln: 0.322 ± 0.176
0.645CysArg: 0.645 ± 0.229
0.564CysSer: 0.564 ± 0.198
0.484CysThr: 0.484 ± 0.216
0.725CysVal: 0.725 ± 0.251
0.161CysTrp: 0.161 ± 0.116
0.242CysTyr: 0.242 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
6.77AspAla: 6.77 ± 0.912
0.645AspCys: 0.645 ± 0.286
3.063AspAsp: 3.063 ± 0.646
3.063AspGlu: 3.063 ± 0.608
1.854AspPhe: 1.854 ± 0.418
5.077AspGly: 5.077 ± 0.678
0.645AspHis: 0.645 ± 0.255
2.74AspIle: 2.74 ± 0.343
2.901AspLys: 2.901 ± 0.523
4.836AspLeu: 4.836 ± 0.887
1.531AspMet: 1.531 ± 0.349
2.015AspAsn: 2.015 ± 0.49
3.788AspPro: 3.788 ± 0.631
2.66AspGln: 2.66 ± 0.505
3.304AspArg: 3.304 ± 0.489
3.224AspSer: 3.224 ± 0.487
2.74AspThr: 2.74 ± 0.469
3.627AspVal: 3.627 ± 0.474
1.37AspTrp: 1.37 ± 0.432
1.773AspTyr: 1.773 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
6.931GluAla: 6.931 ± 1.087
0.564GluCys: 0.564 ± 0.189
2.095GluAsp: 2.095 ± 0.464
2.579GluGlu: 2.579 ± 0.421
1.289GluPhe: 1.289 ± 0.37
3.466GluGly: 3.466 ± 0.503
1.451GluHis: 1.451 ± 0.364
2.257GluIle: 2.257 ± 0.453
2.498GluLys: 2.498 ± 0.479
4.997GluLeu: 4.997 ± 0.756
2.015GluMet: 2.015 ± 0.396
1.773GluAsn: 1.773 ± 0.335
2.176GluPro: 2.176 ± 0.541
3.466GluGln: 3.466 ± 0.627
4.513GluArg: 4.513 ± 0.767
2.337GluSer: 2.337 ± 0.469
2.176GluThr: 2.176 ± 0.255
2.821GluVal: 2.821 ± 0.572
0.806GluTrp: 0.806 ± 0.306
1.289GluTyr: 1.289 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
2.821PheAla: 2.821 ± 0.544
0.161PheCys: 0.161 ± 0.119
1.934PheAsp: 1.934 ± 0.421
1.612PheGlu: 1.612 ± 0.279
1.128PhePhe: 1.128 ± 0.259
3.304PheGly: 3.304 ± 0.432
0.403PheHis: 0.403 ± 0.165
1.209PheIle: 1.209 ± 0.332
1.289PheLys: 1.289 ± 0.277
1.692PheLeu: 1.692 ± 0.22
0.645PheMet: 0.645 ± 0.194
1.37PheAsn: 1.37 ± 0.403
1.37PhePro: 1.37 ± 0.391
1.934PheGln: 1.934 ± 0.453
1.854PheArg: 1.854 ± 0.338
2.579PheSer: 2.579 ± 0.451
1.773PheThr: 1.773 ± 0.449
2.015PheVal: 2.015 ± 0.448
0.725PheTrp: 0.725 ± 0.205
1.048PheTyr: 1.048 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
11.847GlyAla: 11.847 ± 1.085
0.322GlyCys: 0.322 ± 0.137
4.11GlyAsp: 4.11 ± 0.876
4.191GlyGlu: 4.191 ± 0.58
2.579GlyPhe: 2.579 ± 0.42
8.946GlyGly: 8.946 ± 1.374
1.531GlyHis: 1.531 ± 0.441
3.788GlyIle: 3.788 ± 0.456
4.11GlyLys: 4.11 ± 0.539
6.044GlyLeu: 6.044 ± 0.807
2.418GlyMet: 2.418 ± 0.394
3.949GlyAsn: 3.949 ± 0.728
2.176GlyPro: 2.176 ± 0.413
3.466GlyGln: 3.466 ± 0.576
4.594GlyArg: 4.594 ± 0.556
5.4GlySer: 5.4 ± 0.769
5.803GlyThr: 5.803 ± 1.211
5.642GlyVal: 5.642 ± 0.699
1.289GlyTrp: 1.289 ± 0.247
3.143GlyTyr: 3.143 ± 0.5
0.0GlyXaa: 0.0 ± 0.0
His
1.531HisAla: 1.531 ± 0.401
0.242HisCys: 0.242 ± 0.143
0.806HisAsp: 0.806 ± 0.232
0.887HisGlu: 0.887 ± 0.283
0.161HisPhe: 0.161 ± 0.125
1.37HisGly: 1.37 ± 0.382
0.322HisHis: 0.322 ± 0.2
1.209HisIle: 1.209 ± 0.318
0.725HisLys: 0.725 ± 0.263
1.692HisLeu: 1.692 ± 0.561
0.403HisMet: 0.403 ± 0.228
0.484HisAsn: 0.484 ± 0.237
0.967HisPro: 0.967 ± 0.287
0.645HisGln: 0.645 ± 0.186
2.015HisArg: 2.015 ± 0.471
0.725HisSer: 0.725 ± 0.239
0.725HisThr: 0.725 ± 0.233
1.128HisVal: 1.128 ± 0.273
0.484HisTrp: 0.484 ± 0.207
0.322HisTyr: 0.322 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
4.513IleAla: 4.513 ± 0.487
0.322IleCys: 0.322 ± 0.16
3.788IleAsp: 3.788 ± 0.462
2.579IleGlu: 2.579 ± 0.498
1.209IlePhe: 1.209 ± 0.375
4.433IleGly: 4.433 ± 0.686
0.725IleHis: 0.725 ± 0.238
1.531IleIle: 1.531 ± 0.534
2.498IleLys: 2.498 ± 0.38
3.224IleLeu: 3.224 ± 0.506
0.403IleMet: 0.403 ± 0.204
2.337IleAsn: 2.337 ± 0.391
2.176IlePro: 2.176 ± 0.504
1.128IleGln: 1.128 ± 0.268
2.176IleArg: 2.176 ± 0.462
2.74IleSer: 2.74 ± 0.805
3.627IleThr: 3.627 ± 0.887
2.579IleVal: 2.579 ± 0.424
0.806IleTrp: 0.806 ± 0.239
0.887IleTyr: 0.887 ± 0.288
0.0IleXaa: 0.0 ± 0.0
Lys
4.594LysAla: 4.594 ± 0.76
0.484LysCys: 0.484 ± 0.173
2.498LysAsp: 2.498 ± 0.673
2.095LysGlu: 2.095 ± 0.425
1.289LysPhe: 1.289 ± 0.31
2.498LysGly: 2.498 ± 0.431
1.128LysHis: 1.128 ± 0.329
2.015LysIle: 2.015 ± 0.346
1.289LysLys: 1.289 ± 0.328
4.594LysLeu: 4.594 ± 0.525
0.967LysMet: 0.967 ± 0.274
1.773LysAsn: 1.773 ± 0.309
1.531LysPro: 1.531 ± 0.277
2.982LysGln: 2.982 ± 0.604
3.466LysArg: 3.466 ± 0.709
2.257LysSer: 2.257 ± 0.419
2.257LysThr: 2.257 ± 0.414
2.095LysVal: 2.095 ± 0.41
1.128LysTrp: 1.128 ± 0.246
1.209LysTyr: 1.209 ± 0.332
0.0LysXaa: 0.0 ± 0.0
Leu
9.429LeuAla: 9.429 ± 0.87
0.725LeuCys: 0.725 ± 0.235
5.158LeuAsp: 5.158 ± 0.587
3.868LeuGlu: 3.868 ± 0.763
2.579LeuPhe: 2.579 ± 0.594
6.125LeuGly: 6.125 ± 0.646
1.37LeuHis: 1.37 ± 0.385
2.74LeuIle: 2.74 ± 0.503
3.466LeuLys: 3.466 ± 0.601
6.689LeuLeu: 6.689 ± 1.03
2.337LeuMet: 2.337 ± 0.419
2.498LeuAsn: 2.498 ± 0.392
4.271LeuPro: 4.271 ± 0.612
4.836LeuGln: 4.836 ± 0.637
6.447LeuArg: 6.447 ± 0.77
4.594LeuSer: 4.594 ± 0.81
5.642LeuThr: 5.642 ± 0.726
4.836LeuVal: 4.836 ± 0.623
1.37LeuTrp: 1.37 ± 0.373
1.289LeuTyr: 1.289 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
4.191MetAla: 4.191 ± 0.642
0.081MetCys: 0.081 ± 0.098
0.887MetAsp: 0.887 ± 0.194
1.854MetGlu: 1.854 ± 0.35
0.484MetPhe: 0.484 ± 0.187
2.498MetGly: 2.498 ± 0.533
0.242MetHis: 0.242 ± 0.134
0.645MetIle: 0.645 ± 0.239
1.289MetLys: 1.289 ± 0.322
2.257MetLeu: 2.257 ± 0.408
0.887MetMet: 0.887 ± 0.312
0.967MetAsn: 0.967 ± 0.309
1.048MetPro: 1.048 ± 0.291
0.967MetGln: 0.967 ± 0.405
1.451MetArg: 1.451 ± 0.381
1.531MetSer: 1.531 ± 0.305
2.498MetThr: 2.498 ± 0.352
1.612MetVal: 1.612 ± 0.377
0.484MetTrp: 0.484 ± 0.186
0.403MetTyr: 0.403 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
5.722AsnAla: 5.722 ± 0.824
0.403AsnCys: 0.403 ± 0.22
2.176AsnAsp: 2.176 ± 0.375
2.176AsnGlu: 2.176 ± 0.52
1.612AsnPhe: 1.612 ± 0.349
4.352AsnGly: 4.352 ± 1.017
0.887AsnHis: 0.887 ± 0.324
1.854AsnIle: 1.854 ± 0.516
1.612AsnLys: 1.612 ± 0.381
2.901AsnLeu: 2.901 ± 0.574
0.967AsnMet: 0.967 ± 0.274
1.692AsnAsn: 1.692 ± 0.408
1.934AsnPro: 1.934 ± 0.4
1.531AsnGln: 1.531 ± 0.447
1.773AsnArg: 1.773 ± 0.363
2.418AsnSer: 2.418 ± 0.508
2.095AsnThr: 2.095 ± 0.492
2.821AsnVal: 2.821 ± 0.591
0.645AsnTrp: 0.645 ± 0.193
1.289AsnTyr: 1.289 ± 0.341
0.0AsnXaa: 0.0 ± 0.0
Pro
7.092ProAla: 7.092 ± 1.038
0.242ProCys: 0.242 ± 0.133
3.466ProAsp: 3.466 ± 0.616
3.063ProGlu: 3.063 ± 0.583
0.967ProPhe: 0.967 ± 0.318
4.513ProGly: 4.513 ± 0.627
0.564ProHis: 0.564 ± 0.325
1.612ProIle: 1.612 ± 0.408
1.209ProLys: 1.209 ± 0.29
3.546ProLeu: 3.546 ± 0.65
1.37ProMet: 1.37 ± 0.256
1.934ProAsn: 1.934 ± 0.398
2.498ProPro: 2.498 ± 0.471
2.095ProGln: 2.095 ± 0.584
2.337ProArg: 2.337 ± 0.527
2.579ProSer: 2.579 ± 0.521
2.66ProThr: 2.66 ± 0.527
3.224ProVal: 3.224 ± 0.62
0.887ProTrp: 0.887 ± 0.315
1.612ProTyr: 1.612 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
7.495GlnAla: 7.495 ± 0.675
0.161GlnCys: 0.161 ± 0.107
3.143GlnAsp: 3.143 ± 0.499
1.854GlnGlu: 1.854 ± 0.385
1.692GlnPhe: 1.692 ± 0.403
2.821GlnGly: 2.821 ± 0.587
0.725GlnHis: 0.725 ± 0.178
2.498GlnIle: 2.498 ± 0.555
2.015GlnLys: 2.015 ± 0.389
4.836GlnLeu: 4.836 ± 0.685
1.128GlnMet: 1.128 ± 0.282
1.209GlnAsn: 1.209 ± 0.332
1.451GlnPro: 1.451 ± 0.335
2.982GlnGln: 2.982 ± 0.521
4.191GlnArg: 4.191 ± 0.918
2.498GlnSer: 2.498 ± 0.491
2.337GlnThr: 2.337 ± 0.703
2.901GlnVal: 2.901 ± 0.413
1.128GlnTrp: 1.128 ± 0.374
1.37GlnTyr: 1.37 ± 0.34
0.0GlnXaa: 0.0 ± 0.0
Arg
7.656ArgAla: 7.656 ± 1.101
0.564ArgCys: 0.564 ± 0.187
2.418ArgAsp: 2.418 ± 0.441
3.385ArgGlu: 3.385 ± 0.738
2.579ArgPhe: 2.579 ± 0.55
4.916ArgGly: 4.916 ± 0.801
0.887ArgHis: 0.887 ± 0.253
3.546ArgIle: 3.546 ± 0.633
2.579ArgLys: 2.579 ± 0.577
3.627ArgLeu: 3.627 ± 0.544
1.612ArgMet: 1.612 ± 0.329
2.498ArgAsn: 2.498 ± 0.505
2.66ArgPro: 2.66 ± 0.356
2.498ArgGln: 2.498 ± 0.666
4.997ArgArg: 4.997 ± 0.872
2.821ArgSer: 2.821 ± 0.568
3.385ArgThr: 3.385 ± 0.619
5.561ArgVal: 5.561 ± 0.84
1.128ArgTrp: 1.128 ± 0.341
2.337ArgTyr: 2.337 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
7.898SerAla: 7.898 ± 0.815
0.564SerCys: 0.564 ± 0.266
3.063SerAsp: 3.063 ± 0.601
1.692SerGlu: 1.692 ± 0.305
1.451SerPhe: 1.451 ± 0.3
5.964SerGly: 5.964 ± 0.963
0.564SerHis: 0.564 ± 0.184
3.304SerIle: 3.304 ± 0.631
2.176SerLys: 2.176 ± 0.431
3.627SerLeu: 3.627 ± 0.495
2.015SerMet: 2.015 ± 0.309
2.579SerAsn: 2.579 ± 0.523
2.579SerPro: 2.579 ± 0.687
2.74SerGln: 2.74 ± 0.642
2.74SerArg: 2.74 ± 0.592
2.579SerSer: 2.579 ± 0.6
3.385SerThr: 3.385 ± 0.827
4.03SerVal: 4.03 ± 0.709
0.725SerTrp: 0.725 ± 0.256
1.612SerTyr: 1.612 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
7.092ThrAla: 7.092 ± 0.803
0.322ThrCys: 0.322 ± 0.2
3.707ThrAsp: 3.707 ± 0.652
2.982ThrGlu: 2.982 ± 0.499
2.257ThrPhe: 2.257 ± 0.433
5.561ThrGly: 5.561 ± 0.935
0.806ThrHis: 0.806 ± 0.215
2.821ThrIle: 2.821 ± 0.773
2.095ThrLys: 2.095 ± 0.524
5.803ThrLeu: 5.803 ± 0.627
1.451ThrMet: 1.451 ± 0.352
2.095ThrAsn: 2.095 ± 0.666
5.158ThrPro: 5.158 ± 0.588
2.901ThrGln: 2.901 ± 0.624
2.74ThrArg: 2.74 ± 0.5
2.821ThrSer: 2.821 ± 0.499
4.916ThrThr: 4.916 ± 1.193
5.077ThrVal: 5.077 ± 0.894
0.725ThrTrp: 0.725 ± 0.275
1.451ThrTyr: 1.451 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
8.623ValAla: 8.623 ± 0.81
0.725ValCys: 0.725 ± 0.287
4.997ValAsp: 4.997 ± 0.755
4.513ValGlu: 4.513 ± 0.668
2.257ValPhe: 2.257 ± 0.445
4.594ValGly: 4.594 ± 0.721
1.289ValHis: 1.289 ± 0.378
2.498ValIle: 2.498 ± 0.483
2.418ValLys: 2.418 ± 0.387
4.271ValLeu: 4.271 ± 0.79
1.854ValMet: 1.854 ± 0.338
3.466ValAsn: 3.466 ± 0.843
3.063ValPro: 3.063 ± 0.503
2.982ValGln: 2.982 ± 0.514
3.707ValArg: 3.707 ± 0.587
4.191ValSer: 4.191 ± 0.711
4.997ValThr: 4.997 ± 0.603
4.271ValVal: 4.271 ± 0.528
0.725ValTrp: 0.725 ± 0.369
1.209ValTyr: 1.209 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
2.015TrpAla: 2.015 ± 0.39
0.403TrpCys: 0.403 ± 0.185
0.484TrpAsp: 0.484 ± 0.146
1.128TrpGlu: 1.128 ± 0.37
0.725TrpPhe: 0.725 ± 0.289
1.289TrpGly: 1.289 ± 0.328
0.242TrpHis: 0.242 ± 0.128
0.564TrpIle: 0.564 ± 0.175
0.806TrpLys: 0.806 ± 0.234
2.337TrpLeu: 2.337 ± 0.358
0.484TrpMet: 0.484 ± 0.214
0.484TrpAsn: 0.484 ± 0.221
0.161TrpPro: 0.161 ± 0.107
0.887TrpGln: 0.887 ± 0.256
1.048TrpArg: 1.048 ± 0.241
0.967TrpSer: 0.967 ± 0.237
0.806TrpThr: 0.806 ± 0.218
1.048TrpVal: 1.048 ± 0.294
0.564TrpTrp: 0.564 ± 0.183
0.484TrpTyr: 0.484 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.901TyrAla: 2.901 ± 0.488
0.484TyrCys: 0.484 ± 0.186
1.531TyrAsp: 1.531 ± 0.329
1.531TyrGlu: 1.531 ± 0.374
0.484TyrPhe: 0.484 ± 0.193
3.546TyrGly: 3.546 ± 0.458
0.564TyrHis: 0.564 ± 0.214
0.725TyrIle: 0.725 ± 0.231
0.887TyrLys: 0.887 ± 0.215
2.579TyrLeu: 2.579 ± 0.464
0.403TyrMet: 0.403 ± 0.151
1.451TyrAsn: 1.451 ± 0.331
0.967TyrPro: 0.967 ± 0.342
0.967TyrGln: 0.967 ± 0.263
1.531TyrArg: 1.531 ± 0.434
1.934TyrSer: 1.934 ± 0.321
2.095TyrThr: 2.095 ± 0.498
2.095TyrVal: 2.095 ± 0.493
0.564TyrTrp: 0.564 ± 0.188
0.725TyrTyr: 0.725 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12409 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski