Spiroplasma virus SpV1-R8A2 B (SpV1) (Spiroplasma virus 1)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Plectroviridae; Vespertiliovirus; Spiroplasma virus R8A2B

Average proteome isoelectric point is 8.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P15902|ORF11_SPV1R Uncharacterized protein ORF11 OS=Spiroplasma virus SpV1-R8A2 B OX=10854 GN=ORF11 PE=3 SV=2
MM1 pKa = 6.88QNDD4 pKa = 4.29WIKK7 pKa = 10.93LKK9 pKa = 10.81EE10 pKa = 3.75FFIYY14 pKa = 10.24IFLFIDD20 pKa = 3.32KK21 pKa = 9.79TNVEE25 pKa = 5.3SITMWNLTQNEE36 pKa = 4.89YY37 pKa = 9.22LTLMVGVWIVILFLTWFLLWMVFKK61 pKa = 10.78IVGYY65 pKa = 9.59FKK67 pKa = 11.09

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15897|ORF6_SPV1R Uncharacterized protein ORF6 OS=Spiroplasma virus SpV1-R8A2 B OX=10854 GN=ORF6 PE=4 SV=2
MM1 pKa = 7.81INLLVEE7 pKa = 4.5NNNSNWDD14 pKa = 3.76KK15 pKa = 10.97IFSFVFDD22 pKa = 3.42IFLFIFDD29 pKa = 4.91VIWNTKK35 pKa = 10.34LPMTNTSIAYY45 pKa = 8.28FLIFFMVIKK54 pKa = 10.38LSIYY58 pKa = 9.85AIHH61 pKa = 6.33GTSTQYY67 pKa = 11.67NNLGSTVNNGVSQVYY82 pKa = 9.55SSTVRR87 pKa = 11.84GVSDD91 pKa = 4.12TKK93 pKa = 11.03QGMQKK98 pKa = 10.15HH99 pKa = 4.97IKK101 pKa = 8.77EE102 pKa = 3.99RR103 pKa = 11.84KK104 pKa = 6.71QFKK107 pKa = 9.92INRR110 pKa = 11.84NKK112 pKa = 10.34KK113 pKa = 9.24QLSSLAKK120 pKa = 8.57QAKK123 pKa = 7.99TRR125 pKa = 11.84EE126 pKa = 3.66QGYY129 pKa = 9.75RR130 pKa = 11.84RR131 pKa = 11.84VHH133 pKa = 5.84KK134 pKa = 10.71

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

2176

49

715

181.3

21.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.39 ± 0.283

0.643 ± 0.326

4.55 ± 0.634

4.917 ± 0.449

8.594 ± 0.507

4.412 ± 0.377

1.287 ± 0.363

9.926 ± 1.298

10.432 ± 0.987

9.972 ± 0.721

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.471

7.721 ± 1.149

2.068 ± 0.229

3.401 ± 0.573

3.079 ± 0.526

5.928 ± 0.372

4.504 ± 0.496

5.882 ± 0.243

2.482 ± 0.213

5.285 ± 0.78
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski