Measles morbillivirus
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q83624|Q83624_9MONO Matrix protein OS=Measles morbillivirus OX=11234 PE=4 SV=1
MM1 pKa = 8.15 AEE3 pKa = 3.63 EE4 pKa = 3.91 QARR7 pKa = 11.84 HH8 pKa = 4.84 VKK10 pKa = 10.61 NGLEE14 pKa = 4.48 CIRR17 pKa = 11.84 ALKK20 pKa = 10.54 AEE22 pKa = 5.1 PIGSLAIEE30 pKa = 4.18 EE31 pKa = 5.21 AMAAWSEE38 pKa = 4.06 ISDD41 pKa = 3.59 NPGQEE46 pKa = 3.43 RR47 pKa = 11.84 ATCRR51 pKa = 11.84 EE52 pKa = 4.01 EE53 pKa = 4.01 KK54 pKa = 10.5 AGSSGLSKK62 pKa = 10.51 PCLSAIGSTEE72 pKa = 3.52 GGAPRR77 pKa = 11.84 IRR79 pKa = 11.84 GQGPGEE85 pKa = 4.18 SDD87 pKa = 4.51 DD88 pKa = 4.25 DD89 pKa = 4.84 AEE91 pKa = 4.54 TLGIPPRR98 pKa = 11.84 NLQASSTGLQCHH110 pKa = 5.42 YY111 pKa = 10.93 VYY113 pKa = 10.86 DD114 pKa = 4.04 HH115 pKa = 6.87 SGEE118 pKa = 4.12 AVKK121 pKa = 10.71 GIQDD125 pKa = 3.6 ADD127 pKa = 3.98 SIMVQSGLDD136 pKa = 3.52 GDD138 pKa = 4.4 STLSGGDD145 pKa = 3.54 NEE147 pKa = 5.15 SEE149 pKa = 4.21 NSDD152 pKa = 3.37 VDD154 pKa = 3.07 IGEE157 pKa = 4.99 PDD159 pKa = 3.41 TEE161 pKa = 4.81 GYY163 pKa = 10.86 AITDD167 pKa = 3.66 RR168 pKa = 11.84 GSAPISMGFRR178 pKa = 11.84 ASDD181 pKa = 3.44 VEE183 pKa = 4.22 TAEE186 pKa = 4.19 GGEE189 pKa = 3.83 IHH191 pKa = 6.91 EE192 pKa = 4.65 LLRR195 pKa = 11.84 LQSRR199 pKa = 11.84 GNNFPKK205 pKa = 10.39 LGKK208 pKa = 7.92 TLNVPPPPDD217 pKa = 4.04 PGRR220 pKa = 11.84 ASTSGTPIKK229 pKa = 10.51 KK230 pKa = 8.78 GTDD233 pKa = 2.83 ARR235 pKa = 11.84 LASFGTEE242 pKa = 3.5 IASSLTGGATQCARR256 pKa = 11.84 KK257 pKa = 9.94 SPSEE261 pKa = 3.79 PSGPGAPAGNVPEE274 pKa = 4.59 CVSNAALIQEE284 pKa = 4.51 WTPEE288 pKa = 4.01 SGTTISPRR296 pKa = 11.84 SQNNEE301 pKa = 3.9 EE302 pKa = 4.74 GGDD305 pKa = 3.69 HH306 pKa = 6.98 YY307 pKa = 11.6 DD308 pKa = 4.83 DD309 pKa = 4.67 EE310 pKa = 5.32 LFSDD314 pKa = 3.92 VQDD317 pKa = 3.6 IKK319 pKa = 10.35 TALAKK324 pKa = 10.19 IHH326 pKa = 7.01 EE327 pKa = 5.15 DD328 pKa = 3.2 NQKK331 pKa = 10.34 IISKK335 pKa = 10.59 LEE337 pKa = 3.76 SLLLLKK343 pKa = 11.16 GEE345 pKa = 4.15 VEE347 pKa = 4.55 SIKK350 pKa = 10.81 KK351 pKa = 9.72 QINRR355 pKa = 11.84 QNISISTLEE364 pKa = 3.97 GHH366 pKa = 7.29 LSSIMIAIPGLGKK379 pKa = 10.47 DD380 pKa = 4.25 PNDD383 pKa = 3.35 PTADD387 pKa = 3.52 VEE389 pKa = 4.53 INPDD393 pKa = 3.86 LKK395 pKa = 10.89 PIIGRR400 pKa = 11.84 DD401 pKa = 3.18 SGRR404 pKa = 11.84 ALAEE408 pKa = 4.04 VLKK411 pKa = 10.88 KK412 pKa = 10.12 PVASRR417 pKa = 11.84 QLQGMTNGRR426 pKa = 11.84 TSSRR430 pKa = 11.84 GQLLKK435 pKa = 10.65 EE436 pKa = 4.19 FQLKK440 pKa = 10.35 PIGKK444 pKa = 9.26 KK445 pKa = 9.07 MSSAVGFVPDD455 pKa = 4.19 TGPASRR461 pKa = 11.84 SVIRR465 pKa = 11.84 SIIKK469 pKa = 10.0 SSRR472 pKa = 11.84 LEE474 pKa = 3.95 EE475 pKa = 3.99 DD476 pKa = 2.97 RR477 pKa = 11.84 KK478 pKa = 10.54 RR479 pKa = 11.84 YY480 pKa = 10.31 LMTLLDD486 pKa = 5.15 DD487 pKa = 4.11 IKK489 pKa = 11.13 GANDD493 pKa = 3.38 LAKK496 pKa = 10.19 FHH498 pKa = 6.04 QMLMKK503 pKa = 10.09 IIMKK507 pKa = 10.21
Molecular weight: 53.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.124
IPC2_protein 5.092
IPC_protein 5.041
Toseland 4.965
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.156
Wikipedia 4.876
Rodwell 4.927
Grimsley 4.876
Solomon 5.016
Lehninger 4.978
Nozaki 5.13
DTASelect 5.283
Thurlkill 4.952
EMBOSS 4.914
Sillero 5.207
Patrickios 4.444
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.185
Protein with the highest isoelectric point:
>tr|Q83623|Q83623_9MONO Phosphoprotein OS=Measles morbillivirus OX=11234 PE=1 SV=1
MM1 pKa = 7.9 SKK3 pKa = 9.54 TDD5 pKa = 3.3 WNASGLSRR13 pKa = 11.84 PSPSAHH19 pKa = 5.35 WPSRR23 pKa = 11.84 KK24 pKa = 9.12 LWQHH28 pKa = 4.13 GQKK31 pKa = 10.36 YY32 pKa = 7.22 QTTQDD37 pKa = 3.24 RR38 pKa = 11.84 SEE40 pKa = 4.24 PPAGKK45 pKa = 9.52 RR46 pKa = 11.84 RR47 pKa = 11.84 QAVRR51 pKa = 11.84 VSANHH56 pKa = 7.14 ASQQLDD62 pKa = 3.29 QLKK65 pKa = 10.12 AVHH68 pKa = 6.38 LASAVRR74 pKa = 11.84 DD75 pKa = 3.66 LEE77 pKa = 4.45 RR78 pKa = 11.84 AMTTLKK84 pKa = 10.51 LWEE87 pKa = 4.53 SPQEE91 pKa = 4.11 ISRR94 pKa = 11.84 HH95 pKa = 4.01 QALGYY100 pKa = 9.1 SVIMFMITAVKK111 pKa = 10.1 RR112 pKa = 11.84 LRR114 pKa = 11.84 EE115 pKa = 4.28 SKK117 pKa = 10.12 MLTLSWFNQALMVIAPSQEE136 pKa = 3.32 EE137 pKa = 4.5 TMNLKK142 pKa = 8.49 TAMWILANLIPRR154 pKa = 11.84 DD155 pKa = 4.0 MLSLTGDD162 pKa = 4.77 LLPSLWGSGLLMLKK176 pKa = 9.76 LQKK179 pKa = 10.13 EE180 pKa = 4.71 GRR182 pKa = 11.84 STSSS186 pKa = 2.66
Molecular weight: 21.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.198
IPC2_protein 9.487
IPC_protein 9.692
Toseland 10.628
ProMoST 10.145
Dawson 10.701
Bjellqvist 10.306
Wikipedia 10.833
Rodwell 11.125
Grimsley 10.73
Solomon 10.774
Lehninger 10.76
Nozaki 10.599
DTASelect 10.306
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.628
Patrickios 10.877
IPC_peptide 10.789
IPC2_peptide 8.726
IPC2.peptide.svr19 8.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3828
186
2183
765.6
85.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.747 ± 0.575
1.698 ± 0.252
5.59 ± 0.306
5.381 ± 0.5
3.448 ± 0.536
6.479 ± 0.904
2.638 ± 0.509
7.027 ± 0.6
5.486 ± 0.246
10.763 ± 0.569
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.612 ± 0.328
4.31 ± 0.22
4.859 ± 0.569
3.344 ± 0.443
5.773 ± 0.293
8.307 ± 0.645
5.512 ± 0.182
6.348 ± 0.676
1.202 ± 0.296
3.474 ± 0.658
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here