Hubei zhaovirus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KLF6|A0A1L3KLF6_9VIRU RNA-dependent RNA polymerase OS=Hubei zhaovirus-like virus 1 OX=1923344 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.6KK3 pKa = 10.47SVGLRR8 pKa = 11.84KK9 pKa = 9.13QQPKK13 pKa = 7.33MTTVLPATLRR23 pKa = 11.84MKK25 pKa = 10.66NRR27 pKa = 11.84EE28 pKa = 4.14LLSSLSSVSYY38 pKa = 11.51DD39 pKa = 3.08NFEE42 pKa = 4.19LLKK45 pKa = 10.02TYY47 pKa = 10.62KK48 pKa = 10.0LQPGFPLTCPWFASLAQKK66 pKa = 10.04FDD68 pKa = 2.94KK69 pKa = 11.28YY70 pKa = 10.71KK71 pKa = 9.61IHH73 pKa = 6.42NLKK76 pKa = 10.05FCYY79 pKa = 10.02EE80 pKa = 4.36STCPTTTPGRR90 pKa = 11.84IIFLAYY96 pKa = 10.3SDD98 pKa = 5.04PNQQAPVTLYY108 pKa = 9.42EE109 pKa = 4.48AQVSMTNYY117 pKa = 10.47KK118 pKa = 10.21SIQVYY123 pKa = 10.22NYY125 pKa = 10.63GEE127 pKa = 4.1FRR129 pKa = 11.84PDD131 pKa = 2.64QHH133 pKa = 7.46IQMRR137 pKa = 11.84QTEE140 pKa = 4.42FFVRR144 pKa = 11.84SPTTQVVDD152 pKa = 3.32VMKK155 pKa = 10.71YY156 pKa = 10.6DD157 pKa = 3.23IGIMYY162 pKa = 9.76IYY164 pKa = 10.5ADD166 pKa = 4.12TVTQGAVMGNIYY178 pKa = 10.0IEE180 pKa = 3.97YY181 pKa = 9.86DD182 pKa = 3.13ITLMNPVPQRR192 pKa = 11.84DD193 pKa = 3.54TGFSLGTDD201 pKa = 3.78LPKK204 pKa = 10.9LNPFTALDD212 pKa = 3.93YY213 pKa = 11.23NYY215 pKa = 9.17MPSVVQITDD224 pKa = 3.09GNTINLKK231 pKa = 9.89QAGTYY236 pKa = 9.16MLTTAGNVGSGNVITPTFTNVTDD259 pKa = 4.14SVVQAVANSTTGTASGVYY277 pKa = 8.37QVIVTAAQAALAQIVLKK294 pKa = 10.96NYY296 pKa = 8.8AQVMNLTKK304 pKa = 10.58VAA306 pKa = 3.84

Molecular weight:
34.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KLF6|A0A1L3KLF6_9VIRU RNA-dependent RNA polymerase OS=Hubei zhaovirus-like virus 1 OX=1923344 PE=4 SV=1
MM1 pKa = 7.29NSWLIIAWCSLLLLQLAVLIQQRR24 pKa = 11.84TQRR27 pKa = 11.84RR28 pKa = 11.84IVSQTKK34 pKa = 9.54RR35 pKa = 11.84LYY37 pKa = 10.78KK38 pKa = 10.46SGAKK42 pKa = 10.03KK43 pKa = 10.19GVQAPKK49 pKa = 8.3HH50 pKa = 5.27TKK52 pKa = 10.46GSVSKK57 pKa = 10.73DD58 pKa = 2.86KK59 pKa = 10.72PQVRR63 pKa = 11.84KK64 pKa = 7.66TQNQTPVYY72 pKa = 9.09KK73 pKa = 10.45KK74 pKa = 10.02EE75 pKa = 4.44SKK77 pKa = 10.68KK78 pKa = 9.27PTQQQDD84 pKa = 3.08NVYY87 pKa = 10.3KK88 pKa = 10.38KK89 pKa = 9.92EE90 pKa = 4.19SYY92 pKa = 10.43IKK94 pKa = 10.69GPKK97 pKa = 9.14QNVASVIKK105 pKa = 10.55AVIDD109 pKa = 3.43SSKK112 pKa = 11.0YY113 pKa = 9.31EE114 pKa = 3.97EE115 pKa = 4.33TFPTVKK121 pKa = 9.73TNKK124 pKa = 9.52TIEE127 pKa = 4.18LLSNLDD133 pKa = 3.44KK134 pKa = 11.26NINDD138 pKa = 4.35FVLQMTGKK146 pKa = 8.82PVSFKK151 pKa = 10.91VRR153 pKa = 11.84IDD155 pKa = 3.12IDD157 pKa = 3.65GLLDD161 pKa = 3.68YY162 pKa = 10.88DD163 pKa = 3.85NKK165 pKa = 11.13SFDD168 pKa = 4.52GYY170 pKa = 9.86CQPIALYY177 pKa = 9.71IACNWDD183 pKa = 3.72MIQKK187 pKa = 10.0NNSTVITDD195 pKa = 4.38HH196 pKa = 5.19FVKK199 pKa = 10.28WIVNVMTNYY208 pKa = 10.92ANIHH212 pKa = 6.14EE213 pKa = 5.17GFDD216 pKa = 3.51SDD218 pKa = 3.94YY219 pKa = 11.53KK220 pKa = 9.87EE221 pKa = 4.01YY222 pKa = 10.57FYY224 pKa = 11.12FGYY227 pKa = 9.21TEE229 pKa = 3.95IFEE232 pKa = 4.53EE233 pKa = 4.22LATYY237 pKa = 10.17CKK239 pKa = 10.45VSLVRR244 pKa = 11.84LVGVKK249 pKa = 10.08EE250 pKa = 4.18EE251 pKa = 4.67PNQSMFVSGLRR262 pKa = 11.84QCGVQHH268 pKa = 6.21EE269 pKa = 4.49HH270 pKa = 5.64TKK272 pKa = 10.4RR273 pKa = 11.84AIIFMSTDD281 pKa = 2.78KK282 pKa = 10.76NASVGHH288 pKa = 4.96MRR290 pKa = 11.84AYY292 pKa = 10.46VPLIGQGCQPNSLMTDD308 pKa = 2.92KK309 pKa = 10.95DD310 pKa = 3.81FQPPQIKK317 pKa = 9.7IEE319 pKa = 4.18EE320 pKa = 4.41KK321 pKa = 9.4PVEE324 pKa = 4.12QIVEE328 pKa = 4.27KK329 pKa = 10.73IEE331 pKa = 5.32TINQWIGEE339 pKa = 4.37EE340 pKa = 3.81KK341 pKa = 9.88TAFQKK346 pKa = 10.44FLKK349 pKa = 9.91EE350 pKa = 3.7FNIPQVEE357 pKa = 4.3CAKK360 pKa = 10.65LYY362 pKa = 7.94TQAGIDD368 pKa = 3.75YY369 pKa = 9.39AWFFVVPAVVGDD381 pKa = 3.86YY382 pKa = 10.86ASKK385 pKa = 11.27DD386 pKa = 3.16EE387 pKa = 5.79DD388 pKa = 3.31ITVEE392 pKa = 4.0NDD394 pKa = 2.99HH395 pKa = 6.72TSLNIQSLYY404 pKa = 10.97FKK406 pKa = 11.19VNVNQIHH413 pKa = 6.96WDD415 pKa = 3.52NFCPDD420 pKa = 3.24EE421 pKa = 4.85RR422 pKa = 11.84NISVSDD428 pKa = 3.28IYY430 pKa = 11.06YY431 pKa = 9.75TMKK434 pKa = 10.49FAQEE438 pKa = 3.76QPLTRR443 pKa = 11.84RR444 pKa = 11.84RR445 pKa = 11.84LGPNLSVVRR454 pKa = 11.84VQLSNYY460 pKa = 9.09RR461 pKa = 11.84YY462 pKa = 9.35GFPRR466 pKa = 11.84CGSVPSTDD474 pKa = 3.02VVQLVTTFNCHH485 pKa = 5.35ACSVLSRR492 pKa = 11.84AIGYY496 pKa = 9.12KK497 pKa = 7.91RR498 pKa = 11.84TKK500 pKa = 7.73IQRR503 pKa = 11.84YY504 pKa = 5.53TGSRR508 pKa = 11.84YY509 pKa = 10.38DD510 pKa = 3.69FSLKK514 pKa = 9.91TFDD517 pKa = 5.46GKK519 pKa = 10.35NHH521 pKa = 6.64DD522 pKa = 4.03CHH524 pKa = 6.97RR525 pKa = 11.84LSDD528 pKa = 3.53KK529 pKa = 10.09YY530 pKa = 9.52TIIKK534 pKa = 10.16EE535 pKa = 3.79EE536 pKa = 3.86EE537 pKa = 4.3AYY539 pKa = 9.78MKK541 pKa = 10.56QYY543 pKa = 10.95LDD545 pKa = 3.41KK546 pKa = 11.18DD547 pKa = 3.56KK548 pKa = 11.46SEE550 pKa = 5.19KK551 pKa = 10.49SLQSNIGYY559 pKa = 9.37IEE561 pKa = 4.39SGMAYY566 pKa = 10.08YY567 pKa = 10.36RR568 pKa = 11.84YY569 pKa = 9.07LFQDD573 pKa = 2.91IGHH576 pKa = 6.46NNSIRR581 pKa = 11.84PLGLLEE587 pKa = 4.0RR588 pKa = 11.84QRR590 pKa = 11.84FCQLYY595 pKa = 7.85YY596 pKa = 10.65HH597 pKa = 6.08QFVLNIDD604 pKa = 3.7NKK606 pKa = 9.51TLYY609 pKa = 10.64SDD611 pKa = 3.9IMDD614 pKa = 4.23SKK616 pKa = 11.54VKK618 pKa = 9.83TLDD621 pKa = 3.05RR622 pKa = 11.84FPYY625 pKa = 7.59IWKK628 pKa = 9.64VAARR632 pKa = 11.84TINIWLQRR640 pKa = 11.84LFGVLSLLYY649 pKa = 10.15VIYY652 pKa = 10.51GLFVMSFPGFAVQAMSNIGDD672 pKa = 4.1SEE674 pKa = 4.24WYY676 pKa = 9.8AWYY679 pKa = 9.82EE680 pKa = 3.89DD681 pKa = 3.38QFRR684 pKa = 11.84DD685 pKa = 4.08VFHH688 pKa = 7.5PEE690 pKa = 3.79NNNGAVVYY698 pKa = 9.91DD699 pKa = 3.51EE700 pKa = 4.39WEE702 pKa = 3.95QLFKK706 pKa = 11.33GQGTRR711 pKa = 11.84EE712 pKa = 3.91QLLKK716 pKa = 10.35QDD718 pKa = 4.8LYY720 pKa = 11.61TMILTFFGWLSPFIDD735 pKa = 3.78KK736 pKa = 10.86DD737 pKa = 3.72SARR740 pKa = 11.84MLHH743 pKa = 5.87SLAYY747 pKa = 10.46NILNFLLNSNNLFFQAIFGLLKK769 pKa = 9.87IYY771 pKa = 10.36QLLVVIVNVLCFGVDD786 pKa = 3.14PMGPYY791 pKa = 10.03VSSLVLIASHH801 pKa = 6.5VLTILLIRR809 pKa = 11.84YY810 pKa = 8.47IFRR813 pKa = 11.84AFYY816 pKa = 10.79NIMIKK821 pKa = 10.2RR822 pKa = 11.84QFYY825 pKa = 10.82KK826 pKa = 10.27MLVCIFKK833 pKa = 10.94DD834 pKa = 4.7FVAFFFDD841 pKa = 5.4RR842 pKa = 11.84DD843 pKa = 3.71FDD845 pKa = 6.4DD846 pKa = 6.14DD847 pKa = 6.08DD848 pKa = 5.64NDD850 pKa = 3.61LHH852 pKa = 7.89GGAKK856 pKa = 9.64EE857 pKa = 3.87KK858 pKa = 10.96HH859 pKa = 5.93SFNYY863 pKa = 9.89PKK865 pKa = 10.3FNNKK869 pKa = 7.33ITQSVSKK876 pKa = 10.64LNNYY880 pKa = 8.11QNQIIGNDD888 pKa = 2.99VDD890 pKa = 4.29VVHH893 pKa = 6.28TEE895 pKa = 3.81KK896 pKa = 10.33TRR898 pKa = 11.84FEE900 pKa = 3.85QDD902 pKa = 2.68LSKK905 pKa = 10.68KK906 pKa = 10.27GKK908 pKa = 10.19SLVEE912 pKa = 3.96ASKK915 pKa = 9.83DD916 pKa = 3.35TSLKK920 pKa = 10.14RR921 pKa = 11.84DD922 pKa = 3.25EE923 pKa = 4.8KK924 pKa = 10.99LWAKK928 pKa = 10.48LMSVCNCEE936 pKa = 4.14HH937 pKa = 7.29KK938 pKa = 10.24NTLKK942 pKa = 10.04QWKK945 pKa = 8.56LTGQVAYY952 pKa = 10.27EE953 pKa = 4.14NGQKK957 pKa = 9.51IQSCTFSNCLSNLYY971 pKa = 9.85YY972 pKa = 10.47AVHH975 pKa = 6.41ARR977 pKa = 11.84QGGSRR982 pKa = 11.84SSPDD986 pKa = 3.28PYY988 pKa = 10.2VLKK991 pKa = 10.41MFQKK995 pKa = 10.63YY996 pKa = 7.21SRR998 pKa = 11.84KK999 pKa = 9.74QIAIMNEE1006 pKa = 4.11KK1007 pKa = 9.61VKK1009 pKa = 10.55KK1010 pKa = 9.72RR1011 pKa = 11.84YY1012 pKa = 9.22PSFTFNLKK1020 pKa = 10.43SFLDD1024 pKa = 3.54NLPAQKK1030 pKa = 10.09RR1031 pKa = 11.84ALYY1034 pKa = 10.36LRR1036 pKa = 11.84GYY1038 pKa = 10.15NKK1040 pKa = 10.54ALNEE1044 pKa = 4.06HH1045 pKa = 6.56KK1046 pKa = 10.17IEE1048 pKa = 4.11HH1049 pKa = 6.32TMTAMVKK1056 pKa = 9.1QGEE1059 pKa = 4.44VHH1061 pKa = 6.64RR1062 pKa = 11.84VDD1064 pKa = 3.96NNGEE1068 pKa = 3.72ASDD1071 pKa = 3.91RR1072 pKa = 11.84PRR1074 pKa = 11.84VIWNPSEE1081 pKa = 4.06TVQLFVTAFNSVLIQMLKK1099 pKa = 7.45TQIPGFVHH1107 pKa = 6.86GLNKK1111 pKa = 10.33GDD1113 pKa = 4.39LQDD1116 pKa = 4.12KK1117 pKa = 10.17FNAEE1121 pKa = 3.72IPKK1124 pKa = 10.08NRR1126 pKa = 11.84VFLSCDD1132 pKa = 3.23TSGHH1136 pKa = 6.74DD1137 pKa = 3.3ASQYY1141 pKa = 11.37DD1142 pKa = 4.93SLIQCCDD1149 pKa = 2.68NYY1151 pKa = 10.75FIEE1154 pKa = 4.12THH1156 pKa = 5.77YY1157 pKa = 11.54DD1158 pKa = 3.39KK1159 pKa = 10.97IVDD1162 pKa = 3.54ILRR1165 pKa = 11.84DD1166 pKa = 3.65EE1167 pKa = 4.95GNGLDD1172 pKa = 4.23IDD1174 pKa = 4.59HH1175 pKa = 6.77NMSKK1179 pKa = 9.63QLKK1182 pKa = 10.02KK1183 pKa = 11.03YY1184 pKa = 10.14ILSLKK1189 pKa = 7.97TNYY1192 pKa = 8.34MMKK1195 pKa = 10.44YY1196 pKa = 8.06MNTIIEE1202 pKa = 4.38RR1203 pKa = 11.84GTIKK1207 pKa = 9.92GTVFSGHH1214 pKa = 5.5ATRR1217 pKa = 11.84TTFGNSLRR1225 pKa = 11.84VYY1227 pKa = 9.24MYY1229 pKa = 10.49ISFALSFLTDD1239 pKa = 2.99IQGYY1243 pKa = 8.31FNIYY1247 pKa = 10.16VSGDD1251 pKa = 3.55DD1252 pKa = 3.5VLIACRR1258 pKa = 11.84EE1259 pKa = 3.74QDD1261 pKa = 3.42VQKK1264 pKa = 10.81IQDD1267 pKa = 3.2QLYY1270 pKa = 9.54RR1271 pKa = 11.84YY1272 pKa = 9.88VYY1274 pKa = 10.2LKK1276 pKa = 10.88KK1277 pKa = 10.59EE1278 pKa = 4.1GVGALGQVCKK1288 pKa = 10.74SVIYY1292 pKa = 10.24QKK1294 pKa = 10.86SSYY1297 pKa = 11.09SFLSCCGVVSSSGHH1311 pKa = 6.17SITRR1315 pKa = 11.84QPKK1318 pKa = 8.54RR1319 pKa = 11.84ALLSGLNTRR1328 pKa = 11.84KK1329 pKa = 10.3SISVKK1334 pKa = 9.7DD1335 pKa = 3.76HH1336 pKa = 5.71YY1337 pKa = 11.18NSRR1340 pKa = 11.84VYY1342 pKa = 10.55QILSWCKK1349 pKa = 10.29DD1350 pKa = 3.4LLPFYY1355 pKa = 10.53MLTLKK1360 pKa = 10.82NQDD1363 pKa = 3.54SEE1365 pKa = 4.65DD1366 pKa = 4.99DD1367 pKa = 3.4IVKK1370 pKa = 9.83TMDD1373 pKa = 3.87YY1374 pKa = 9.55SWKK1377 pKa = 7.82MHH1379 pKa = 5.0QNKK1382 pKa = 9.54RR1383 pKa = 11.84PIDD1386 pKa = 3.68KK1387 pKa = 10.8YY1388 pKa = 11.44AVFQQWIVEE1397 pKa = 4.32LQDD1400 pKa = 3.12QAYY1403 pKa = 7.81TFFDD1407 pKa = 3.24IDD1409 pKa = 3.81FNQPTFNLVAGSVCILRR1426 pKa = 11.84FPNNEE1431 pKa = 3.67QCVVGG1436 pKa = 4.43

Molecular weight:
166.48 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1742

306

1436

871.0

100.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.592 ± 1.027

1.837 ± 0.388

5.798 ± 0.701

3.904 ± 0.583

5.224 ± 0.589

4.937 ± 0.132

1.952 ± 0.587

6.429 ± 0.543

7.635 ± 1.089

8.324 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.606

6.028 ± 0.066

3.387 ± 0.981

6.257 ± 0.274

3.904 ± 0.583

6.889 ± 0.455

5.913 ± 2.352

7.577 ± 0.564

1.148 ± 0.372

5.683 ± 0.238

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski