Hubei zhaovirus-like virus 1
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLF6|A0A1L3KLF6_9VIRU RNA-dependent RNA polymerase OS=Hubei zhaovirus-like virus 1 OX=1923344 PE=4 SV=1
MM1 pKa = 7.39 EE2 pKa = 5.6 KK3 pKa = 10.47 SVGLRR8 pKa = 11.84 KK9 pKa = 9.13 QQPKK13 pKa = 7.33 MTTVLPATLRR23 pKa = 11.84 MKK25 pKa = 10.66 NRR27 pKa = 11.84 EE28 pKa = 4.14 LLSSLSSVSYY38 pKa = 11.51 DD39 pKa = 3.08 NFEE42 pKa = 4.19 LLKK45 pKa = 10.02 TYY47 pKa = 10.62 KK48 pKa = 10.0 LQPGFPLTCPWFASLAQKK66 pKa = 10.04 FDD68 pKa = 2.94 KK69 pKa = 11.28 YY70 pKa = 10.71 KK71 pKa = 9.61 IHH73 pKa = 6.42 NLKK76 pKa = 10.05 FCYY79 pKa = 10.02 EE80 pKa = 4.36 STCPTTTPGRR90 pKa = 11.84 IIFLAYY96 pKa = 10.3 SDD98 pKa = 5.04 PNQQAPVTLYY108 pKa = 9.42 EE109 pKa = 4.48 AQVSMTNYY117 pKa = 10.47 KK118 pKa = 10.21 SIQVYY123 pKa = 10.22 NYY125 pKa = 10.63 GEE127 pKa = 4.1 FRR129 pKa = 11.84 PDD131 pKa = 2.64 QHH133 pKa = 7.46 IQMRR137 pKa = 11.84 QTEE140 pKa = 4.42 FFVRR144 pKa = 11.84 SPTTQVVDD152 pKa = 3.32 VMKK155 pKa = 10.71 YY156 pKa = 10.6 DD157 pKa = 3.23 IGIMYY162 pKa = 9.76 IYY164 pKa = 10.5 ADD166 pKa = 4.12 TVTQGAVMGNIYY178 pKa = 10.0 IEE180 pKa = 3.97 YY181 pKa = 9.86 DD182 pKa = 3.13 ITLMNPVPQRR192 pKa = 11.84 DD193 pKa = 3.54 TGFSLGTDD201 pKa = 3.78 LPKK204 pKa = 10.9 LNPFTALDD212 pKa = 3.93 YY213 pKa = 11.23 NYY215 pKa = 9.17 MPSVVQITDD224 pKa = 3.09 GNTINLKK231 pKa = 9.89 QAGTYY236 pKa = 9.16 MLTTAGNVGSGNVITPTFTNVTDD259 pKa = 4.14 SVVQAVANSTTGTASGVYY277 pKa = 8.37 QVIVTAAQAALAQIVLKK294 pKa = 10.96 NYY296 pKa = 8.8 AQVMNLTKK304 pKa = 10.58 VAA306 pKa = 3.84
Molecular weight: 34.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.736
IPC2_protein 7.834
IPC_protein 7.849
Toseland 7.775
ProMoST 8.214
Dawson 8.463
Bjellqvist 8.565
Wikipedia 8.492
Rodwell 8.478
Grimsley 7.658
Solomon 8.639
Lehninger 8.609
Nozaki 8.551
DTASelect 8.434
Thurlkill 8.463
EMBOSS 8.639
Sillero 8.712
Patrickios 3.401
IPC_peptide 8.624
IPC2_peptide 7.439
IPC2.peptide.svr19 7.756
Protein with the highest isoelectric point:
>tr|A0A1L3KLF6|A0A1L3KLF6_9VIRU RNA-dependent RNA polymerase OS=Hubei zhaovirus-like virus 1 OX=1923344 PE=4 SV=1
MM1 pKa = 7.29 NSWLIIAWCSLLLLQLAVLIQQRR24 pKa = 11.84 TQRR27 pKa = 11.84 RR28 pKa = 11.84 IVSQTKK34 pKa = 9.54 RR35 pKa = 11.84 LYY37 pKa = 10.78 KK38 pKa = 10.46 SGAKK42 pKa = 10.03 KK43 pKa = 10.19 GVQAPKK49 pKa = 8.3 HH50 pKa = 5.27 TKK52 pKa = 10.46 GSVSKK57 pKa = 10.73 DD58 pKa = 2.86 KK59 pKa = 10.72 PQVRR63 pKa = 11.84 KK64 pKa = 7.66 TQNQTPVYY72 pKa = 9.09 KK73 pKa = 10.45 KK74 pKa = 10.02 EE75 pKa = 4.44 SKK77 pKa = 10.68 KK78 pKa = 9.27 PTQQQDD84 pKa = 3.08 NVYY87 pKa = 10.3 KK88 pKa = 10.38 KK89 pKa = 9.92 EE90 pKa = 4.19 SYY92 pKa = 10.43 IKK94 pKa = 10.69 GPKK97 pKa = 9.14 QNVASVIKK105 pKa = 10.55 AVIDD109 pKa = 3.43 SSKK112 pKa = 11.0 YY113 pKa = 9.31 EE114 pKa = 3.97 EE115 pKa = 4.33 TFPTVKK121 pKa = 9.73 TNKK124 pKa = 9.52 TIEE127 pKa = 4.18 LLSNLDD133 pKa = 3.44 KK134 pKa = 11.26 NINDD138 pKa = 4.35 FVLQMTGKK146 pKa = 8.82 PVSFKK151 pKa = 10.91 VRR153 pKa = 11.84 IDD155 pKa = 3.12 IDD157 pKa = 3.65 GLLDD161 pKa = 3.68 YY162 pKa = 10.88 DD163 pKa = 3.85 NKK165 pKa = 11.13 SFDD168 pKa = 4.52 GYY170 pKa = 9.86 CQPIALYY177 pKa = 9.71 IACNWDD183 pKa = 3.72 MIQKK187 pKa = 10.0 NNSTVITDD195 pKa = 4.38 HH196 pKa = 5.19 FVKK199 pKa = 10.28 WIVNVMTNYY208 pKa = 10.92 ANIHH212 pKa = 6.14 EE213 pKa = 5.17 GFDD216 pKa = 3.51 SDD218 pKa = 3.94 YY219 pKa = 11.53 KK220 pKa = 9.87 EE221 pKa = 4.01 YY222 pKa = 10.57 FYY224 pKa = 11.12 FGYY227 pKa = 9.21 TEE229 pKa = 3.95 IFEE232 pKa = 4.53 EE233 pKa = 4.22 LATYY237 pKa = 10.17 CKK239 pKa = 10.45 VSLVRR244 pKa = 11.84 LVGVKK249 pKa = 10.08 EE250 pKa = 4.18 EE251 pKa = 4.67 PNQSMFVSGLRR262 pKa = 11.84 QCGVQHH268 pKa = 6.21 EE269 pKa = 4.49 HH270 pKa = 5.64 TKK272 pKa = 10.4 RR273 pKa = 11.84 AIIFMSTDD281 pKa = 2.78 KK282 pKa = 10.76 NASVGHH288 pKa = 4.96 MRR290 pKa = 11.84 AYY292 pKa = 10.46 VPLIGQGCQPNSLMTDD308 pKa = 2.92 KK309 pKa = 10.95 DD310 pKa = 3.81 FQPPQIKK317 pKa = 9.7 IEE319 pKa = 4.18 EE320 pKa = 4.41 KK321 pKa = 9.4 PVEE324 pKa = 4.12 QIVEE328 pKa = 4.27 KK329 pKa = 10.73 IEE331 pKa = 5.32 TINQWIGEE339 pKa = 4.37 EE340 pKa = 3.81 KK341 pKa = 9.88 TAFQKK346 pKa = 10.44 FLKK349 pKa = 9.91 EE350 pKa = 3.7 FNIPQVEE357 pKa = 4.3 CAKK360 pKa = 10.65 LYY362 pKa = 7.94 TQAGIDD368 pKa = 3.75 YY369 pKa = 9.39 AWFFVVPAVVGDD381 pKa = 3.86 YY382 pKa = 10.86 ASKK385 pKa = 11.27 DD386 pKa = 3.16 EE387 pKa = 5.79 DD388 pKa = 3.31 ITVEE392 pKa = 4.0 NDD394 pKa = 2.99 HH395 pKa = 6.72 TSLNIQSLYY404 pKa = 10.97 FKK406 pKa = 11.19 VNVNQIHH413 pKa = 6.96 WDD415 pKa = 3.52 NFCPDD420 pKa = 3.24 EE421 pKa = 4.85 RR422 pKa = 11.84 NISVSDD428 pKa = 3.28 IYY430 pKa = 11.06 YY431 pKa = 9.75 TMKK434 pKa = 10.49 FAQEE438 pKa = 3.76 QPLTRR443 pKa = 11.84 RR444 pKa = 11.84 RR445 pKa = 11.84 LGPNLSVVRR454 pKa = 11.84 VQLSNYY460 pKa = 9.09 RR461 pKa = 11.84 YY462 pKa = 9.35 GFPRR466 pKa = 11.84 CGSVPSTDD474 pKa = 3.02 VVQLVTTFNCHH485 pKa = 5.35 ACSVLSRR492 pKa = 11.84 AIGYY496 pKa = 9.12 KK497 pKa = 7.91 RR498 pKa = 11.84 TKK500 pKa = 7.73 IQRR503 pKa = 11.84 YY504 pKa = 5.53 TGSRR508 pKa = 11.84 YY509 pKa = 10.38 DD510 pKa = 3.69 FSLKK514 pKa = 9.91 TFDD517 pKa = 5.46 GKK519 pKa = 10.35 NHH521 pKa = 6.64 DD522 pKa = 4.03 CHH524 pKa = 6.97 RR525 pKa = 11.84 LSDD528 pKa = 3.53 KK529 pKa = 10.09 YY530 pKa = 9.52 TIIKK534 pKa = 10.16 EE535 pKa = 3.79 EE536 pKa = 3.86 EE537 pKa = 4.3 AYY539 pKa = 9.78 MKK541 pKa = 10.56 QYY543 pKa = 10.95 LDD545 pKa = 3.41 KK546 pKa = 11.18 DD547 pKa = 3.56 KK548 pKa = 11.46 SEE550 pKa = 5.19 KK551 pKa = 10.49 SLQSNIGYY559 pKa = 9.37 IEE561 pKa = 4.39 SGMAYY566 pKa = 10.08 YY567 pKa = 10.36 RR568 pKa = 11.84 YY569 pKa = 9.07 LFQDD573 pKa = 2.91 IGHH576 pKa = 6.46 NNSIRR581 pKa = 11.84 PLGLLEE587 pKa = 4.0 RR588 pKa = 11.84 QRR590 pKa = 11.84 FCQLYY595 pKa = 7.85 YY596 pKa = 10.65 HH597 pKa = 6.08 QFVLNIDD604 pKa = 3.7 NKK606 pKa = 9.51 TLYY609 pKa = 10.64 SDD611 pKa = 3.9 IMDD614 pKa = 4.23 SKK616 pKa = 11.54 VKK618 pKa = 9.83 TLDD621 pKa = 3.05 RR622 pKa = 11.84 FPYY625 pKa = 7.59 IWKK628 pKa = 9.64 VAARR632 pKa = 11.84 TINIWLQRR640 pKa = 11.84 LFGVLSLLYY649 pKa = 10.15 VIYY652 pKa = 10.51 GLFVMSFPGFAVQAMSNIGDD672 pKa = 4.1 SEE674 pKa = 4.24 WYY676 pKa = 9.8 AWYY679 pKa = 9.82 EE680 pKa = 3.89 DD681 pKa = 3.38 QFRR684 pKa = 11.84 DD685 pKa = 4.08 VFHH688 pKa = 7.5 PEE690 pKa = 3.79 NNNGAVVYY698 pKa = 9.91 DD699 pKa = 3.51 EE700 pKa = 4.39 WEE702 pKa = 3.95 QLFKK706 pKa = 11.33 GQGTRR711 pKa = 11.84 EE712 pKa = 3.91 QLLKK716 pKa = 10.35 QDD718 pKa = 4.8 LYY720 pKa = 11.61 TMILTFFGWLSPFIDD735 pKa = 3.78 KK736 pKa = 10.86 DD737 pKa = 3.72 SARR740 pKa = 11.84 MLHH743 pKa = 5.87 SLAYY747 pKa = 10.46 NILNFLLNSNNLFFQAIFGLLKK769 pKa = 9.87 IYY771 pKa = 10.36 QLLVVIVNVLCFGVDD786 pKa = 3.14 PMGPYY791 pKa = 10.03 VSSLVLIASHH801 pKa = 6.5 VLTILLIRR809 pKa = 11.84 YY810 pKa = 8.47 IFRR813 pKa = 11.84 AFYY816 pKa = 10.79 NIMIKK821 pKa = 10.2 RR822 pKa = 11.84 QFYY825 pKa = 10.82 KK826 pKa = 10.27 MLVCIFKK833 pKa = 10.94 DD834 pKa = 4.7 FVAFFFDD841 pKa = 5.4 RR842 pKa = 11.84 DD843 pKa = 3.71 FDD845 pKa = 6.4 DD846 pKa = 6.14 DD847 pKa = 6.08 DD848 pKa = 5.64 NDD850 pKa = 3.61 LHH852 pKa = 7.89 GGAKK856 pKa = 9.64 EE857 pKa = 3.87 KK858 pKa = 10.96 HH859 pKa = 5.93 SFNYY863 pKa = 9.89 PKK865 pKa = 10.3 FNNKK869 pKa = 7.33 ITQSVSKK876 pKa = 10.64 LNNYY880 pKa = 8.11 QNQIIGNDD888 pKa = 2.99 VDD890 pKa = 4.29 VVHH893 pKa = 6.28 TEE895 pKa = 3.81 KK896 pKa = 10.33 TRR898 pKa = 11.84 FEE900 pKa = 3.85 QDD902 pKa = 2.68 LSKK905 pKa = 10.68 KK906 pKa = 10.27 GKK908 pKa = 10.19 SLVEE912 pKa = 3.96 ASKK915 pKa = 9.83 DD916 pKa = 3.35 TSLKK920 pKa = 10.14 RR921 pKa = 11.84 DD922 pKa = 3.25 EE923 pKa = 4.8 KK924 pKa = 10.99 LWAKK928 pKa = 10.48 LMSVCNCEE936 pKa = 4.14 HH937 pKa = 7.29 KK938 pKa = 10.24 NTLKK942 pKa = 10.04 QWKK945 pKa = 8.56 LTGQVAYY952 pKa = 10.27 EE953 pKa = 4.14 NGQKK957 pKa = 9.51 IQSCTFSNCLSNLYY971 pKa = 9.85 YY972 pKa = 10.47 AVHH975 pKa = 6.41 ARR977 pKa = 11.84 QGGSRR982 pKa = 11.84 SSPDD986 pKa = 3.28 PYY988 pKa = 10.2 VLKK991 pKa = 10.41 MFQKK995 pKa = 10.63 YY996 pKa = 7.21 SRR998 pKa = 11.84 KK999 pKa = 9.74 QIAIMNEE1006 pKa = 4.11 KK1007 pKa = 9.61 VKK1009 pKa = 10.55 KK1010 pKa = 9.72 RR1011 pKa = 11.84 YY1012 pKa = 9.22 PSFTFNLKK1020 pKa = 10.43 SFLDD1024 pKa = 3.54 NLPAQKK1030 pKa = 10.09 RR1031 pKa = 11.84 ALYY1034 pKa = 10.36 LRR1036 pKa = 11.84 GYY1038 pKa = 10.15 NKK1040 pKa = 10.54 ALNEE1044 pKa = 4.06 HH1045 pKa = 6.56 KK1046 pKa = 10.17 IEE1048 pKa = 4.11 HH1049 pKa = 6.32 TMTAMVKK1056 pKa = 9.1 QGEE1059 pKa = 4.44 VHH1061 pKa = 6.64 RR1062 pKa = 11.84 VDD1064 pKa = 3.96 NNGEE1068 pKa = 3.72 ASDD1071 pKa = 3.91 RR1072 pKa = 11.84 PRR1074 pKa = 11.84 VIWNPSEE1081 pKa = 4.06 TVQLFVTAFNSVLIQMLKK1099 pKa = 7.45 TQIPGFVHH1107 pKa = 6.86 GLNKK1111 pKa = 10.33 GDD1113 pKa = 4.39 LQDD1116 pKa = 4.12 KK1117 pKa = 10.17 FNAEE1121 pKa = 3.72 IPKK1124 pKa = 10.08 NRR1126 pKa = 11.84 VFLSCDD1132 pKa = 3.23 TSGHH1136 pKa = 6.74 DD1137 pKa = 3.3 ASQYY1141 pKa = 11.37 DD1142 pKa = 4.93 SLIQCCDD1149 pKa = 2.68 NYY1151 pKa = 10.75 FIEE1154 pKa = 4.12 THH1156 pKa = 5.77 YY1157 pKa = 11.54 DD1158 pKa = 3.39 KK1159 pKa = 10.97 IVDD1162 pKa = 3.54 ILRR1165 pKa = 11.84 DD1166 pKa = 3.65 EE1167 pKa = 4.95 GNGLDD1172 pKa = 4.23 IDD1174 pKa = 4.59 HH1175 pKa = 6.77 NMSKK1179 pKa = 9.63 QLKK1182 pKa = 10.02 KK1183 pKa = 11.03 YY1184 pKa = 10.14 ILSLKK1189 pKa = 7.97 TNYY1192 pKa = 8.34 MMKK1195 pKa = 10.44 YY1196 pKa = 8.06 MNTIIEE1202 pKa = 4.38 RR1203 pKa = 11.84 GTIKK1207 pKa = 9.92 GTVFSGHH1214 pKa = 5.5 ATRR1217 pKa = 11.84 TTFGNSLRR1225 pKa = 11.84 VYY1227 pKa = 9.24 MYY1229 pKa = 10.49 ISFALSFLTDD1239 pKa = 2.99 IQGYY1243 pKa = 8.31 FNIYY1247 pKa = 10.16 VSGDD1251 pKa = 3.55 DD1252 pKa = 3.5 VLIACRR1258 pKa = 11.84 EE1259 pKa = 3.74 QDD1261 pKa = 3.42 VQKK1264 pKa = 10.81 IQDD1267 pKa = 3.2 QLYY1270 pKa = 9.54 RR1271 pKa = 11.84 YY1272 pKa = 9.88 VYY1274 pKa = 10.2 LKK1276 pKa = 10.88 KK1277 pKa = 10.59 EE1278 pKa = 4.1 GVGALGQVCKK1288 pKa = 10.74 SVIYY1292 pKa = 10.24 QKK1294 pKa = 10.86 SSYY1297 pKa = 11.09 SFLSCCGVVSSSGHH1311 pKa = 6.17 SITRR1315 pKa = 11.84 QPKK1318 pKa = 8.54 RR1319 pKa = 11.84 ALLSGLNTRR1328 pKa = 11.84 KK1329 pKa = 10.3 SISVKK1334 pKa = 9.7 DD1335 pKa = 3.76 HH1336 pKa = 5.71 YY1337 pKa = 11.18 NSRR1340 pKa = 11.84 VYY1342 pKa = 10.55 QILSWCKK1349 pKa = 10.29 DD1350 pKa = 3.4 LLPFYY1355 pKa = 10.53 MLTLKK1360 pKa = 10.82 NQDD1363 pKa = 3.54 SEE1365 pKa = 4.65 DD1366 pKa = 4.99 DD1367 pKa = 3.4 IVKK1370 pKa = 9.83 TMDD1373 pKa = 3.87 YY1374 pKa = 9.55 SWKK1377 pKa = 7.82 MHH1379 pKa = 5.0 QNKK1382 pKa = 9.54 RR1383 pKa = 11.84 PIDD1386 pKa = 3.68 KK1387 pKa = 10.8 YY1388 pKa = 11.44 AVFQQWIVEE1397 pKa = 4.32 LQDD1400 pKa = 3.12 QAYY1403 pKa = 7.81 TFFDD1407 pKa = 3.24 IDD1409 pKa = 3.81 FNQPTFNLVAGSVCILRR1426 pKa = 11.84 FPNNEE1431 pKa = 3.67 QCVVGG1436 pKa = 4.43
Molecular weight: 166.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.006
IPC2_protein 8.156
IPC_protein 7.936
Toseland 8.009
ProMoST 8.521
Dawson 8.799
Bjellqvist 8.916
Wikipedia 8.858
Rodwell 8.887
Grimsley 8.185
Solomon 8.829
Lehninger 8.814
Nozaki 9.004
DTASelect 8.726
Thurlkill 8.799
EMBOSS 8.946
Sillero 9.048
Patrickios 4.139
IPC_peptide 8.814
IPC2_peptide 7.658
IPC2.peptide.svr19 7.855
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1742
306
1436
871.0
100.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.592 ± 1.027
1.837 ± 0.388
5.798 ± 0.701
3.904 ± 0.583
5.224 ± 0.589
4.937 ± 0.132
1.952 ± 0.587
6.429 ± 0.543
7.635 ± 1.089
8.324 ± 0.217
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.583 ± 0.606
6.028 ± 0.066
3.387 ± 0.981
6.257 ± 0.274
3.904 ± 0.583
6.889 ± 0.455
5.913 ± 2.352
7.577 ± 0.564
1.148 ± 0.372
5.683 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here