Rhizobium album
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2DY48|A0A2U2DY48_9RHIZ DUF3563 domain-containing protein OS=Rhizobium album OX=2182425 GN=DEM27_03525 PE=4 SV=1
MM1 pKa = 7.49 NIKK4 pKa = 10.39 SLLLGSAAALAVVSGAQAADD24 pKa = 4.21 AIVAAEE30 pKa = 4.47 PEE32 pKa = 3.93 PMEE35 pKa = 4.1 YY36 pKa = 10.8 VRR38 pKa = 11.84 VCDD41 pKa = 4.42 AFGTGYY47 pKa = 10.47 FYY49 pKa = 10.57 IPGTEE54 pKa = 3.81 TCLKK58 pKa = 9.41 VGGRR62 pKa = 11.84 VRR64 pKa = 11.84 FDD66 pKa = 3.06 VQAADD71 pKa = 3.78 SYY73 pKa = 11.85 SPNSDD78 pKa = 3.37 NGWNTRR84 pKa = 11.84 SRR86 pKa = 11.84 AEE88 pKa = 4.51 LYY90 pKa = 10.68 LDD92 pKa = 3.62 SASDD96 pKa = 3.65 TEE98 pKa = 4.38 YY99 pKa = 11.35 GALKK103 pKa = 8.16 TQIVARR109 pKa = 11.84 WDD111 pKa = 3.24 FDD113 pKa = 3.64 GDD115 pKa = 4.15 YY116 pKa = 11.32 NDD118 pKa = 4.64 GQHH121 pKa = 5.47 TTSKK125 pKa = 10.77 LIAANITLAGFLVGLADD142 pKa = 3.52 SQYY145 pKa = 11.16 TSFTGYY151 pKa = 10.87 AGDD154 pKa = 4.93 IINDD158 pKa = 3.53 DD159 pKa = 3.83 VIAYY163 pKa = 10.22 GEE165 pKa = 4.32 FEE167 pKa = 4.53 VNQISYY173 pKa = 9.12 TYY175 pKa = 10.7 DD176 pKa = 3.14 GGNGFTAVISLEE188 pKa = 4.18 DD189 pKa = 3.87 DD190 pKa = 3.31 SRR192 pKa = 11.84 TGDD195 pKa = 3.51 DD196 pKa = 3.62 YY197 pKa = 11.53 MVDD200 pKa = 3.46 VVGGIGYY207 pKa = 7.79 STGTFGFKK215 pKa = 10.49 VVGGYY220 pKa = 10.12 DD221 pKa = 3.36 EE222 pKa = 4.6 SAEE225 pKa = 4.02 EE226 pKa = 4.13 GAIKK230 pKa = 10.53 ARR232 pKa = 11.84 LDD234 pKa = 3.72 GNFGALSAFLMAGWNSDD251 pKa = 2.61 GDD253 pKa = 4.37 VVHH256 pKa = 7.01 KK257 pKa = 8.36 FAPSNFGGAAAGIGWGDD274 pKa = 3.33 WAVWGGLGYY283 pKa = 10.66 SFSDD287 pKa = 3.2 TLAANLQLAYY297 pKa = 9.65 TDD299 pKa = 4.09 SEE301 pKa = 4.78 VFAAAANVKK310 pKa = 8.52 WNPVPGLLIQPEE322 pKa = 4.04 ITYY325 pKa = 8.19 TNWDD329 pKa = 4.52 SIDD332 pKa = 3.5 EE333 pKa = 4.3 DD334 pKa = 3.35 QWNGMVRR341 pKa = 11.84 FQRR344 pKa = 11.84 TFF346 pKa = 2.79
Molecular weight: 36.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.897
IPC_protein 3.923
Toseland 3.694
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.605
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.745
EMBOSS 3.859
Sillero 4.037
Patrickios 1.291
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A2U2DRW5|A0A2U2DRW5_9RHIZ Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE OS=Rhizobium album OX=2182425 GN=ubiE PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATNGGQKK29 pKa = 9.78 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6427
0
6427
1993445
23
2835
310.2
33.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.965 ± 0.038
0.829 ± 0.008
5.789 ± 0.025
5.832 ± 0.024
3.899 ± 0.02
8.412 ± 0.023
2.003 ± 0.015
5.636 ± 0.023
3.688 ± 0.023
9.869 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.583 ± 0.016
2.816 ± 0.016
4.781 ± 0.02
3.132 ± 0.019
6.766 ± 0.032
5.718 ± 0.02
5.393 ± 0.018
7.316 ± 0.026
1.272 ± 0.012
2.303 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here